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The map label for this gene is nrtB-1

Identifier: 11497706

GI number: 11497706

Start: 83680

End: 84414

Strand: Reverse

Name: nrtB-1

Synonym: AF0086

Alternate gene names: 11497706

Gene position: 84414-83680 (Counterclockwise)

Preceding gene: 11497707

Following gene: 11497704

Centisome position: 3.88

GC content: 50.07

Gene sequence:

>735_bases
ATGCAAGCTTCTAAGGCAGACTATAAGGGCTACCTGATAACCTTAGCTTCCCTGCTTCTCATCTGGGCGGCTGCAAGCTA
CTTACTGCAAAATCCCGCCCTGCCCAATCCACTGGACGTGCTGGCTTTAATCTTAACTCGCAAAGAGCTGATAACGCACA
CAGCCGTGAGCTTGATCAGAGTCGTGTACTCTCTCGCGCTTGCCCTCTCAGTTGCCCTTCCTGCTGGCATTTTAAGCCGC
GAAAGAGTTGTTGATGCAACAATCTCGCCGATAATATACCTTCTCTATCCCATCCCCCACATTGTTCTTCTTCCCCTCTA
CATTCTTCTCTTCGGGATAGGTGACCTGTCAAGAGTTCTGCTCATCGCAACAATCCTTTTTTTCCAGATTGCCGTCACAA
CCAGAGATGCGGCAAAGCAGGTCAGCGATTACTACGTTTACTCAATACTCTCACTCGGAGCGTCAAAAATCGACATTTAC
AGGCACGTAATCATCCCAGCTGTTATGCCGAAAATCCTGACTGCGTTGAGAATAAGCATAGGAACCGCCATAGCAGTCCT
GTTCTTTGCTGAGAGCTTCGCCACAACGAGCGGTCTGGGATACCTCATAATCGACTCCTGGAGCAGGGCCGACTACACGA
CAATGTACGCTGCAATTACAACAATGGCCCTTCTCGGCTTTGCACTGTACGTCATCGTTGAGAGCGCCGAGAGGAGAGTC
TGCAGGTGGCTCTAA

Upstream 100 bases:

>100_bases
TAGTGGTTCTTACCGAGAGACCGGGAAGGGTGAAGGCGGTCGTGGATAACAGAGAGGCTGGGGATGAGAGTTACAGATAC
GAGGAGGTGTTTTTCGAAAG

Downstream 100 bases:

>100_bases
GTCCAAATCCATTATTCACAAGCCAGAGCGACTCCGGAATGTGAATCAGAAACCAGTGATAGCTGTAAAAACAGGTGGGA
TTTCCGAGTCGAAAAGGAGG

Product: nitrate ABC transporter, permease protein (nrtB-1)

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MQASKADYKGYLITLASLLLIWAAASYLLQNPALPNPLDVLALILTRKELITHTAVSLIRVVYSLALALSVALPAGILSR
ERVVDATISPIIYLLYPIPHIVLLPLYILLFGIGDLSRVLLIATILFFQIAVTTRDAAKQVSDYYVYSILSLGASKIDIY
RHVIIPAVMPKILTALRISIGTAIAVLFFAESFATTSGLGYLIIDSWSRADYTTMYAAITTMALLGFALYVIVESAERRV
CRWL

Sequences:

>Translated_244_residues
MQASKADYKGYLITLASLLLIWAAASYLLQNPALPNPLDVLALILTRKELITHTAVSLIRVVYSLALALSVALPAGILSR
ERVVDATISPIIYLLYPIPHIVLLPLYILLFGIGDLSRVLLIATILFFQIAVTTRDAAKQVSDYYVYSILSLGASKIDIY
RHVIIPAVMPKILTALRISIGTAIAVLFFAESFATTSGLGYLIIDSWSRADYTTMYAAITTMALLGFALYVIVESAERRV
CRWL
>Mature_244_residues
MQASKADYKGYLITLASLLLIWAAASYLLQNPALPNPLDVLALILTRKELITHTAVSLIRVVYSLALALSVALPAGILSR
ERVVDATISPIIYLLYPIPHIVLLPLYILLFGIGDLSRVLLIATILFFQIAVTTRDAAKQVSDYYVYSILSLGASKIDIY
RHVIIPAVMPKILTALRISIGTAIAVLFFAESFATTSGLGYLIIDSWSRADYTTMYAAITTMALLGFALYVIVESAERRV
CRWL

Specific function: Part of a binding-protein-dependent transport system for taurine. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=241, Percent_Identity=33.6099585062241, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87081802, Length=163, Percent_Identity=31.9018404907975, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26825; Mature: 26825

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASKADYKGYLITLASLLLIWAAASYLLQNPALPNPLDVLALILTRKELITHTAVSLIR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVYSLALALSVALPAGILSRERVVDATISPIIYLLYPIPHIVLLPLYILLFGIGDLSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIATILFFQIAVTTRDAAKQVSDYYVYSILSLGASKIDIYRHVIIPAVMPKILTALRISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GTAIAVLFFAESFATTSGLGYLIIDSWSRADYTTMYAAITTMALLGFALYVIVESAERRV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRWL
HHCC
>Mature Secondary Structure
MQASKADYKGYLITLASLLLIWAAASYLLQNPALPNPLDVLALILTRKELITHTAVSLIR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VVYSLALALSVALPAGILSRERVVDATISPIIYLLYPIPHIVLLPLYILLFGIGDLSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LIATILFFQIAVTTRDAAKQVSDYYVYSILSLGASKIDIYRHVIIPAVMPKILTALRISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GTAIAVLFFAESFATTSGLGYLIIDSWSRADYTTMYAAITTMALLGFALYVIVESAERRV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CRWL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8808933; 9278503 [H]