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The map label for this gene is dapE-1

Identifier: 11497671

GI number: 11497671

Start: 58049

End: 59158

Strand: Reverse

Name: dapE-1

Synonym: AF0051

Alternate gene names: 11497671

Gene position: 59158-58049 (Counterclockwise)

Preceding gene: 11497673

Following gene: 11497669

Centisome position: 2.72

GC content: 51.26

Gene sequence:

>1110_bases
ATGGACCCCCTTGAGATAACCAAAGACCTCATAAAAATAGACACCCGCAACCCTCCCGGAGTGACTACTGAGGCTGTCGA
GTATCTTTCACAGCTTTTCTCGTCCTACGAGCAAAGGATTTACGCGAAGGAGGAGGGTAAGGAGAATCTCGTTGTTTACA
TCAGCAGGGGCAAACCCGAAATCATGCTCACCTCCCACCTCGATACCGTTCCTGCTGGAGACGAGCTTCTGAACCCCGTC
ATTGTTGACGGAAAGCTCTACGGGAGGGGGAGTTGTGATGCGAAGGGCTGTGTTGCGGCAATTTGCTCAGCCTCGCAGAT
AGAGCCTGAGTGCGGGCTGAAGCTGGCCTTCACCTCAGATGAAGAGGTTGGAGGGGTTAATGGGCTCGGATATGTTTTTG
AAAGGGAAAAGGCTGATGCAGTCATAATAGGGGAGCCGACGGGGAGTGAGAGCATCGGAGTTTTGCAAGCTGCCGTTTTG
GCGTTGGATATAGAGTTCAAGGGAAATTCGGGGCACACAGCCTCTCACGACGCTAAGGAGGGGGCGATATACAGAGCCTC
CGAGTACATCGTTGAGAAAGTTGAATCCTTCAGAGGGCTTGAGGGGGATTTTGGGAGCTATAGGGAAATTTTCAGCAAGC
TCGGCATGGATTTTGCTGTTAAAAGCTGGCATGCGGTCTTCAATCCTTCTATGATTAGGGGAGGGGTTAAGAGGAATGTT
GTTGCTCCGAAGTGCACGGTTTACGCCGACGTAAGATTCGCCCCGTGGATAAGCGTTGAAGAAGTGAGAAGAGAGCTTTA
CGCCGAGAACATGGAGTTCAGGGTGGAGGGCTTTCTGCAGCCCTACGGTGTTGGCTGCGATGCTGTTAAGCTTGAGGATG
ACTTAAGGCTGCTGAAAATTATGAGCGAGGCAATAAGGGAAGAGGGATTGAGGCCGAAGGCTGTGTTTTCCTTGGGTGTT
GGAGATACGAGGCACGTCAGAAAGCACGGAGTTCCCGCATTCTACTTGGGTCCGGGTGGGGGGAATTTGCACGGTGAGGA
CGAGTTCGTTTACGTGGAGGAGCTTTACAGAACGGCAAAAATTTACAAAAATATTGTCAGAAGGTTCTGA

Upstream 100 bases:

>100_bases
TCTTTCGCATAAAATCATGTAATTCACTATCATTATGATAGTTTTTGGAATCAAAAGGGATAAAGGGCAACAGCTATAAC
AATGCCTACCCACTCAACTC

Downstream 100 bases:

>100_bases
CCTTCCCCTCAATAACCATGTCAGTTATCTTGCCAACGTTGGCAATCATCTCCTCTGCAATGTCCCTTGCGGGCCTTTCG
AGCTTTGAAATGTCGTAGCC

Product: succinyl-diaminopimelate desuccinylase (dapE-1)

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDPLEITKDLIKIDTRNPPGVTTEAVEYLSQLFSSYEQRIYAKEEGKENLVVYISRGKPEIMLTSHLDTVPAGDELLNPV
IVDGKLYGRGSCDAKGCVAAICSASQIEPECGLKLAFTSDEEVGGVNGLGYVFEREKADAVIIGEPTGSESIGVLQAAVL
ALDIEFKGNSGHTASHDAKEGAIYRASEYIVEKVESFRGLEGDFGSYREIFSKLGMDFAVKSWHAVFNPSMIRGGVKRNV
VAPKCTVYADVRFAPWISVEEVRRELYAENMEFRVEGFLQPYGVGCDAVKLEDDLRLLKIMSEAIREEGLRPKAVFSLGV
GDTRHVRKHGVPAFYLGPGGGNLHGEDEFVYVEELYRTAKIYKNIVRRF

Sequences:

>Translated_369_residues
MDPLEITKDLIKIDTRNPPGVTTEAVEYLSQLFSSYEQRIYAKEEGKENLVVYISRGKPEIMLTSHLDTVPAGDELLNPV
IVDGKLYGRGSCDAKGCVAAICSASQIEPECGLKLAFTSDEEVGGVNGLGYVFEREKADAVIIGEPTGSESIGVLQAAVL
ALDIEFKGNSGHTASHDAKEGAIYRASEYIVEKVESFRGLEGDFGSYREIFSKLGMDFAVKSWHAVFNPSMIRGGVKRNV
VAPKCTVYADVRFAPWISVEEVRRELYAENMEFRVEGFLQPYGVGCDAVKLEDDLRLLKIMSEAIREEGLRPKAVFSLGV
GDTRHVRKHGVPAFYLGPGGGNLHGEDEFVYVEELYRTAKIYKNIVRRF
>Mature_369_residues
MDPLEITKDLIKIDTRNPPGVTTEAVEYLSQLFSSYEQRIYAKEEGKENLVVYISRGKPEIMLTSHLDTVPAGDELLNPV
IVDGKLYGRGSCDAKGCVAAICSASQIEPECGLKLAFTSDEEVGGVNGLGYVFEREKADAVIIGEPTGSESIGVLQAAVL
ALDIEFKGNSGHTASHDAKEGAIYRASEYIVEKVESFRGLEGDFGSYREIFSKLGMDFAVKSWHAVFNPSMIRGGVKRNV
VAPKCTVYADVRFAPWISVEEVRRELYAENMEFRVEGFLQPYGVGCDAVKLEDDLRLLKIMSEAIREEGLRPKAVFSLGV
GDTRHVRKHGVPAFYLGPGGGNLHGEDEFVYVEELYRTAKIYKNIVRRF

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=398, Percent_Identity=25.6281407035176, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790395, Length=254, Percent_Identity=26.3779527559055, Blast_Score=62, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24649208, Length=397, Percent_Identity=23.4256926952141, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24649210, Length=400, Percent_Identity=24.5, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24649202, Length=403, Percent_Identity=23.8213399503722, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24649204, Length=403, Percent_Identity=23.8213399503722, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24649212, Length=404, Percent_Identity=21.7821782178218, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24649206, Length=402, Percent_Identity=23.8805970149254, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40781; Mature: 40781

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLEITKDLIKIDTRNPPGVTTEAVEYLSQLFSSYEQRIYAKEEGKENLVVYISRGKPE
CCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCEEEEEECCCCC
IMLTSHLDTVPAGDELLNPVIVDGKLYGRGSCDAKGCVAAICSASQIEPECGLKLAFTSD
EEEEECCCCCCCCHHHCCCEEECCEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCC
EEVGGVNGLGYVFEREKADAVIIGEPTGSESIGVLQAAVLALDIEFKGNSGHTASHDAKE
CCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHEEEEEEEEEECCCCCCCCCCCCC
GAIYRASEYIVEKVESFRGLEGDFGSYREIFSKLGMDFAVKSWHAVFNPSMIRGGVKRNV
CCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCC
VAPKCTVYADVRFAPWISVEEVRRELYAENMEFRVEGFLQPYGVGCDAVKLEDDLRLLKI
CCCCEEEEEECEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHH
MSEAIREEGLRPKAVFSLGVGDTRHVRKHGVPAFYLGPGGGNLHGEDEFVYVEELYRTAK
HHHHHHHCCCCCHHEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHH
IYKNIVRRF
HHHHHHHCC
>Mature Secondary Structure
MDPLEITKDLIKIDTRNPPGVTTEAVEYLSQLFSSYEQRIYAKEEGKENLVVYISRGKPE
CCHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHEEHHCCCCCCEEEEEECCCCC
IMLTSHLDTVPAGDELLNPVIVDGKLYGRGSCDAKGCVAAICSASQIEPECGLKLAFTSD
EEEEECCCCCCCCHHHCCCEEECCEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCC
EEVGGVNGLGYVFEREKADAVIIGEPTGSESIGVLQAAVLALDIEFKGNSGHTASHDAKE
CCCCCCCCCCCEEECCCCCEEEEECCCCCCHHHHHHHEEEEEEEEEECCCCCCCCCCCCC
GAIYRASEYIVEKVESFRGLEGDFGSYREIFSKLGMDFAVKSWHAVFNPSMIRGGVKRNV
CCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCC
VAPKCTVYADVRFAPWISVEEVRRELYAENMEFRVEGFLQPYGVGCDAVKLEDDLRLLKI
CCCCEEEEEECEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHH
MSEAIREEGLRPKAVFSLGVGDTRHVRKHGVPAFYLGPGGGNLHGEDEFVYVEELYRTAK
HHHHHHHCCCCCHHEEEECCCCHHHHHHCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHH
IYKNIVRRF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA