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The map label for this gene is pmbA [H]

Identifier: 114800402

GI number: 114800402

Start: 1393000

End: 1394346

Strand: Direct

Name: pmbA [H]

Synonym: HNE_1364

Alternate gene names: 114800402

Gene position: 1393000-1394346 (Clockwise)

Preceding gene: 114799093

Following gene: 114799663

Centisome position: 37.6

GC content: 65.92

Gene sequence:

>1347_bases
ATGGCCGATTCACAGATTTCCCCCGAAAATACCCTCTCTGACCTGCTCGACCAGTGCCGCAAGGCCGGGGCCGACGCCGC
CGACGCCCGCCTCGGAATCGCCGATGGCGTCAGCGTTTCCGTGCGTGACGGCAGGCTCGAAAGCATCGAACGCGAGGAGA
GCGCCAGCATCGCCCTGCGCTGCTTCTACGGCAAGCGTCAGGCCAGCGTCTCCGGCGCCGACCTCAGCCGCGAGGGTCTC
AAAGCCCTCGCCGAACGCTGCGCCGCCATGGCAAGGGCCGTGCCCGAAGATAAATTTTGCGGCCTGCCAGATCCCTCCGA
ATTGATCGCTGGGCCCGCAGATCTGGACCTTTCCGGCGAGCCTGAAATCTCCGCCGAAACGCTCGAACGTGAAGCGCTGG
CTGCAGAAGCCGCCGCCCTGGCCGTGCCGGGCGTGAAGACCGTGGCAGGCTGTGGCACCTCCTGGAACCGCTCCACCCGC
TGGGTCGCCGCCACCAACGGCTTCAGCGCCTGGAAGACCGGCACCTCCACCAGCCTCGGCCTCTCCGCTGTGGCCGAAAA
GAACGGCCAGATGGAGCGCGACTATGACAGCTGGTCTGTCCGCTTCACCAAGGACCGCCCCTCGGCTGAAGAGATCGGCC
GCACGGCCGGTGAGCGCACCATCGCCCGCCTCGGCGCCCGCAAGATCGCCACCCAGAAAGCCCCGGTTATCTTCGACCGG
CGCGTGTCTGACAGCCTGATCGGCGCGCTGCTGGGCGCCATCTCTGGCCCCTCCATCGCGCGCGGTGTCAGCTTCCTGAA
GGACCGGATGGACCAGCAGGTGTTCGCCAAGGGCATCTACATCACCGACGACCCGTTCCGCCCGCTGGGCATGGGCAGCC
GCGTGCATGATGGCGAGGGCCTGCCCGTTGCCGAGACCCACCTCATCGAGGACGGCAGGCTGACCACCTGGCTGCTGAAC
CTGCCGTCTGCGCGCCAGCTGGGCCTCAAATCCAACGGCTTTGCTTCGCTCGGCTTTGGTGATCCGCCAGGCGTCTCCAC
GTCCAACGTCTATCTGCGCCCCGGCAAGGACAGTCCCGGCCAGCTCGCCAAGCAGATTGGCAAGGGCCTGCTGGTGACGG
ACATGTTCGGCCCGTCGATCAACCCGAATACGGGCGACTATTCGGTTGGCGTTGCCGGTTTCTGGATTGAGGATGGCGAA
ATTGCCTGGCCTGTCTCCGAAGTGACCGTGGCCGGCGATCTGCCGTCCATGTTTGCCCGGATGGTGCCCGCCAATGACCT
GGAATTACGCTCCACACGCGACGCCCCGTCGATCCTCATCGAGGACATGAACCTTGCAGGCAGCTGA

Upstream 100 bases:

>100_bases
CTGGTTTGTCCAAGTCTGTCCGGGGAATGTCCCGGTTTGTCCTGGAATGGGTGGCACCGTGCCTGTTGCGCGCAGGCCCG
GTGCGGTCCATTTACCGGTG

Downstream 100 bases:

>100_bases
ACGAAGCCTTGATCAGGATCTTCAGCTCATGCTGGCGCTCGCCAAGGAGGCGGGCGACCTTGCTCTATCCTACACGCTGA
CGGGCGGGGCGGAGGCCTGG

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MADSQISPENTLSDLLDQCRKAGADAADARLGIADGVSVSVRDGRLESIEREESASIALRCFYGKRQASVSGADLSREGL
KALAERCAAMARAVPEDKFCGLPDPSELIAGPADLDLSGEPEISAETLEREALAAEAAALAVPGVKTVAGCGTSWNRSTR
WVAATNGFSAWKTGTSTSLGLSAVAEKNGQMERDYDSWSVRFTKDRPSAEEIGRTAGERTIARLGARKIATQKAPVIFDR
RVSDSLIGALLGAISGPSIARGVSFLKDRMDQQVFAKGIYITDDPFRPLGMGSRVHDGEGLPVAETHLIEDGRLTTWLLN
LPSARQLGLKSNGFASLGFGDPPGVSTSNVYLRPGKDSPGQLAKQIGKGLLVTDMFGPSINPNTGDYSVGVAGFWIEDGE
IAWPVSEVTVAGDLPSMFARMVPANDLELRSTRDAPSILIEDMNLAGS

Sequences:

>Translated_448_residues
MADSQISPENTLSDLLDQCRKAGADAADARLGIADGVSVSVRDGRLESIEREESASIALRCFYGKRQASVSGADLSREGL
KALAERCAAMARAVPEDKFCGLPDPSELIAGPADLDLSGEPEISAETLEREALAAEAAALAVPGVKTVAGCGTSWNRSTR
WVAATNGFSAWKTGTSTSLGLSAVAEKNGQMERDYDSWSVRFTKDRPSAEEIGRTAGERTIARLGARKIATQKAPVIFDR
RVSDSLIGALLGAISGPSIARGVSFLKDRMDQQVFAKGIYITDDPFRPLGMGSRVHDGEGLPVAETHLIEDGRLTTWLLN
LPSARQLGLKSNGFASLGFGDPPGVSTSNVYLRPGKDSPGQLAKQIGKGLLVTDMFGPSINPNTGDYSVGVAGFWIEDGE
IAWPVSEVTVAGDLPSMFARMVPANDLELRSTRDAPSILIEDMNLAGS
>Mature_447_residues
ADSQISPENTLSDLLDQCRKAGADAADARLGIADGVSVSVRDGRLESIEREESASIALRCFYGKRQASVSGADLSREGLK
ALAERCAAMARAVPEDKFCGLPDPSELIAGPADLDLSGEPEISAETLEREALAAEAAALAVPGVKTVAGCGTSWNRSTRW
VAATNGFSAWKTGTSTSLGLSAVAEKNGQMERDYDSWSVRFTKDRPSAEEIGRTAGERTIARLGARKIATQKAPVIFDRR
VSDSLIGALLGAISGPSIARGVSFLKDRMDQQVFAKGIYITDDPFRPLGMGSRVHDGEGLPVAETHLIEDGRLTTWLLNL
PSARQLGLKSNGFASLGFGDPPGVSTSNVYLRPGKDSPGQLAKQIGKGLLVTDMFGPSINPNTGDYSVGVAGFWIEDGEI
AWPVSEVTVAGDLPSMFARMVPANDLELRSTRDAPSILIEDMNLAGS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=426, Percent_Identity=34.9765258215962, Blast_Score=237, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47400; Mature: 47269

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSQISPENTLSDLLDQCRKAGADAADARLGIADGVSVSVRDGRLESIEREESASIALR
CCCCCCCCHHHHHHHHHHHHHCCCCHHHCEEEECCCCEEEEECCCHHHHHHHHCCCEEEE
CFYGKRQASVSGADLSREGLKALAERCAAMARAVPEDKFCGLPDPSELIAGPADLDLSGE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCC
PEISAETLEREALAAEAAALAVPGVKTVAGCGTSWNRSTRWVAATNGFSAWKTGTSTSLG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHCCCCCCCCC
LSAVAEKNGQMERDYDSWSVRFTKDRPSAEEIGRTAGERTIARLGARKIATQKAPVIFDR
HHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
RVSDSLIGALLGAISGPSIARGVSFLKDRMDQQVFAKGIYITDDPFRPLGMGSRVHDGEG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCC
LPVAETHLIEDGRLTTWLLNLPSARQLGLKSNGFASLGFGDPPGVSTSNVYLRPGKDSPG
CCCHHHEEECCCCEEEEEEECCCHHHCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCHH
QLAKQIGKGLLVTDMFGPSINPNTGDYSVGVAGFWIEDGEIAWPVSEVTVAGDLPSMFAR
HHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCEECCHHHEEECCCHHHHHHH
MVPANDLELRSTRDAPSILIEDMNLAGS
HCCCCCCEECCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
ADSQISPENTLSDLLDQCRKAGADAADARLGIADGVSVSVRDGRLESIEREESASIALR
CCCCCCCHHHHHHHHHHHHHCCCCHHHCEEEECCCCEEEEECCCHHHHHHHHCCCEEEE
CFYGKRQASVSGADLSREGLKALAERCAAMARAVPEDKFCGLPDPSELIAGPADLDLSGE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCC
PEISAETLEREALAAEAAALAVPGVKTVAGCGTSWNRSTRWVAATNGFSAWKTGTSTSLG
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECCCCCHHCCCCCCCCC
LSAVAEKNGQMERDYDSWSVRFTKDRPSAEEIGRTAGERTIARLGARKIATQKAPVIFDR
HHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
RVSDSLIGALLGAISGPSIARGVSFLKDRMDQQVFAKGIYITDDPFRPLGMGSRVHDGEG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCC
LPVAETHLIEDGRLTTWLLNLPSARQLGLKSNGFASLGFGDPPGVSTSNVYLRPGKDSPG
CCCHHHEEECCCCEEEEEEECCCHHHCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCHH
QLAKQIGKGLLVTDMFGPSINPNTGDYSVGVAGFWIEDGEIAWPVSEVTVAGDLPSMFAR
HHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCEECCHHHEEECCCHHHHHHH
MVPANDLELRSTRDAPSILIEDMNLAGS
HCCCCCCEECCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]