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The map label for this gene is glnA [H]

Identifier: 114800384

GI number: 114800384

Start: 2156598

End: 2158001

Strand: Direct

Name: glnA [H]

Synonym: HNE_2084

Alternate gene names: 114800384

Gene position: 2156598-2158001 (Clockwise)

Preceding gene: 114797943

Following gene: 114797373

Centisome position: 58.21

GC content: 59.54

Gene sequence:

>1404_bases
ATGGCAGACGATCTTCTCAAGCTTATGAAGGACCAGGACGTCCAGTATGTGGACCTGCGCTTCACCGACACGCGCGGCAA
GCTGCAGCACGTGTCCTTCCACAAGGACATGGTGGACAAGAGCTTCTTCGAAGATGGCCAGATGTTTGACGGCTCCTCGA
TTGCTGGCTGGAAAGCCATCAACGAGTCCGACATGCTTCTGAAACCGGACGCGAAAGGCGCGTTCATCGACCCGTTCTTC
CAACAGACCACCCTTGTTGTGTTCTGTGACATTCTCGACCCGATCTCGGGCCAGGCTTACAGCCGCGACCCGCGCACGAC
CGCCAAGAAGGCAGAGGCTTACCTCGCCGCCTCCGGCGTTGGCGACACCGCATTCTTCGGCCCGGAAGCTGAGTTCTTCA
TCTTTGACGACGTGCGCTGGTCAACCGACCCGCACAACACGGGCTACTCGTTCGATTCGACCGAACTGCCGGCGAATTCC
GGCCGCGAATATCCGATGGGCAACCTCGGCCACCGCCCCGGCCCGAAGGGCGGCTATTTCCCCGTTCCGCCAATCGACTC
CGAGCAGGACATGCGCGGCGAGATGCTGTCGATCATGGGTGACATCGGCCTTGAGCCAGAGAAGCACCACCATGAAGTGG
CCCCGGCCCAGCACGAGCTTGGCCTGAAATTCTCGACGCTCACGGTCATGGCTGACCGGATGCAGCTCTACAAATACGTG
ATCCACCAGGTCGCCGCCTCTTACGGCAAAACCGCGACCTTCATGCCCAAACCGATGTTCAAGGACAACGGCTCGGGCAT
GCACGTTCACCAGTCGATCTGGAAAGGCGGCAAGCCGCTCTTCGCAGGCGACAAGTATGCTGATCTCTCCGAGATGTGCC
TCTACTATATCGGCGGCATCATCAAACACGCCAAGGCTCTGAACGCGATCACCAACCCATCGACCAACTCGTACAAGCGA
CTGGTCCCCGGCTATGAAGCGCCTGTGATGCTGGCTTATTCGGCACGCAACCGTTCGGCCTCGATCCGTATTCCTTACGG
CTCGAACCCGAAAGCAAAGCGTATCGAAACCCGCTTCCCTGACCCGATGGCGAACCCCTACCTCGCCTTCGCAGCCCTGC
TGATGGCTGGCCTCGACGGGATCGAGAACAAGATCCATCCGGGCGACGCGATGGACAAGGACCTCTACGACCTGCCACCG
GCAGAAGCCAAGCAGATCCCGCAGGTCTGCGGCTCGCTGCGTGAAGCCCTGGCGGCCCTCGATGCCGACCGCGCCTTCCT
CAAGAAAGGCGGCGTGTTCGACGACGACCAGATCGATGCCTACATCGAACTGAAGATGGAAGAGAACATGCGTTACGAGC
TTCATCCGCACCCGGTTGAGTTCGACATGTACTACAAGAACTAG

Upstream 100 bases:

>100_bases
ACAGGCGACTCGGCGCTTTGATCGATCCCTGCGGGGCCGGCGTCTGACGCAGGCCCCGCAAAACCCCGCACCGAAAAACG
CTCATTAGAAGGCAAAAAAA

Downstream 100 bases:

>100_bases
ACCGCGGGGGTCTTGTTCTGGGCAGAGGCCGGCATCTTCGGGTGCCGGCCTTTTCCTTGCCGCAAAGGCGTGTCACGTCA
TCGCGCATGACCTTCCTTAA

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MADDLLKLMKDQDVQYVDLRFTDTRGKLQHVSFHKDMVDKSFFEDGQMFDGSSIAGWKAINESDMLLKPDAKGAFIDPFF
QQTTLVVFCDILDPISGQAYSRDPRTTAKKAEAYLAASGVGDTAFFGPEAEFFIFDDVRWSTDPHNTGYSFDSTELPANS
GREYPMGNLGHRPGPKGGYFPVPPIDSEQDMRGEMLSIMGDIGLEPEKHHHEVAPAQHELGLKFSTLTVMADRMQLYKYV
IHQVAASYGKTATFMPKPMFKDNGSGMHVHQSIWKGGKPLFAGDKYADLSEMCLYYIGGIIKHAKALNAITNPSTNSYKR
LVPGYEAPVMLAYSARNRSASIRIPYGSNPKAKRIETRFPDPMANPYLAFAALLMAGLDGIENKIHPGDAMDKDLYDLPP
AEAKQIPQVCGSLREALAALDADRAFLKKGGVFDDDQIDAYIELKMEENMRYELHPHPVEFDMYYKN

Sequences:

>Translated_467_residues
MADDLLKLMKDQDVQYVDLRFTDTRGKLQHVSFHKDMVDKSFFEDGQMFDGSSIAGWKAINESDMLLKPDAKGAFIDPFF
QQTTLVVFCDILDPISGQAYSRDPRTTAKKAEAYLAASGVGDTAFFGPEAEFFIFDDVRWSTDPHNTGYSFDSTELPANS
GREYPMGNLGHRPGPKGGYFPVPPIDSEQDMRGEMLSIMGDIGLEPEKHHHEVAPAQHELGLKFSTLTVMADRMQLYKYV
IHQVAASYGKTATFMPKPMFKDNGSGMHVHQSIWKGGKPLFAGDKYADLSEMCLYYIGGIIKHAKALNAITNPSTNSYKR
LVPGYEAPVMLAYSARNRSASIRIPYGSNPKAKRIETRFPDPMANPYLAFAALLMAGLDGIENKIHPGDAMDKDLYDLPP
AEAKQIPQVCGSLREALAALDADRAFLKKGGVFDDDQIDAYIELKMEENMRYELHPHPVEFDMYYKN
>Mature_466_residues
ADDLLKLMKDQDVQYVDLRFTDTRGKLQHVSFHKDMVDKSFFEDGQMFDGSSIAGWKAINESDMLLKPDAKGAFIDPFFQ
QTTLVVFCDILDPISGQAYSRDPRTTAKKAEAYLAASGVGDTAFFGPEAEFFIFDDVRWSTDPHNTGYSFDSTELPANSG
REYPMGNLGHRPGPKGGYFPVPPIDSEQDMRGEMLSIMGDIGLEPEKHHHEVAPAQHELGLKFSTLTVMADRMQLYKYVI
HQVAASYGKTATFMPKPMFKDNGSGMHVHQSIWKGGKPLFAGDKYADLSEMCLYYIGGIIKHAKALNAITNPSTNSYKRL
VPGYEAPVMLAYSARNRSASIRIPYGSNPKAKRIETRFPDPMANPYLAFAALLMAGLDGIENKIHPGDAMDKDLYDLPPA
EAKQIPQVCGSLREALAALDADRAFLKKGGVFDDDQIDAYIELKMEENMRYELHPHPVEFDMYYKN

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=379, Percent_Identity=24.0105540897098, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1790301, Length=465, Percent_Identity=64.7311827956989, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=176, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52181; Mature: 52050

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDLLKLMKDQDVQYVDLRFTDTRGKLQHVSFHKDMVDKSFFEDGQMFDGSSIAGWKAI
CHHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEECCCCCCCCEEC
NESDMLLKPDAKGAFIDPFFQQTTLVVFCDILDPISGQAYSRDPRTTAKKAEAYLAASGV
CCCCCEECCCCCCCEECHHHHHCEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHEEECCC
GDTAFFGPEAEFFIFDDVRWSTDPHNTGYSFDSTELPANSGREYPMGNLGHRPGPKGGYF
CCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPPIDSEQDMRGEMLSIMGDIGLEPEKHHHEVAPAQHELGLKFSTLTVMADRMQLYKYV
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCEEHHHHHHHHHHHHHHHH
IHQVAASYGKTATFMPKPMFKDNGSGMHVHQSIWKGGKPLFAGDKYADLSEMCLYYIGGI
HHHHHHHHCCCEECCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
IKHAKALNAITNPSTNSYKRLVPGYEAPVMLAYSARNRSASIRIPYGSNPKAKRIETRFP
HHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC
DPMANPYLAFAALLMAGLDGIENKIHPGDAMDKDLYDLPPAEAKQIPQVCGSLREALAAL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
DADRAFLKKGGVFDDDQIDAYIELKMEENMRYELHPHPVEFDMYYKN
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEEEEECC
>Mature Secondary Structure 
ADDLLKLMKDQDVQYVDLRFTDTRGKLQHVSFHKDMVDKSFFEDGQMFDGSSIAGWKAI
HHHHHHHHHCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCEECCCCCCCCEEC
NESDMLLKPDAKGAFIDPFFQQTTLVVFCDILDPISGQAYSRDPRTTAKKAEAYLAASGV
CCCCCEECCCCCCCEECHHHHHCEEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHEEECCC
GDTAFFGPEAEFFIFDDVRWSTDPHNTGYSFDSTELPANSGREYPMGNLGHRPGPKGGYF
CCCCEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPPIDSEQDMRGEMLSIMGDIGLEPEKHHHEVAPAQHELGLKFSTLTVMADRMQLYKYV
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHCCEEHHHHHHHHHHHHHHHH
IHQVAASYGKTATFMPKPMFKDNGSGMHVHQSIWKGGKPLFAGDKYADLSEMCLYYIGGI
HHHHHHHHCCCEECCCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
IKHAKALNAITNPSTNSYKRLVPGYEAPVMLAYSARNRSASIRIPYGSNPKAKRIETRFP
HHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC
DPMANPYLAFAALLMAGLDGIENKIHPGDAMDKDLYDLPPAEAKQIPQVCGSLREALAAL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHH
DADRAFLKKGGVFDDDQIDAYIELKMEENMRYELHPHPVEFDMYYKN
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]