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The map label for this gene is hslU

Identifier: 114800330

GI number: 114800330

Start: 2110902

End: 2112206

Strand: Direct

Name: hslU

Synonym: HNE_2034

Alternate gene names: 114800330

Gene position: 2110902-2112206 (Clockwise)

Preceding gene: 114798299

Following gene: 114798888

Centisome position: 56.97

GC content: 63.3

Gene sequence:

>1305_bases
ATGAAACACCTGACGCCCCGCGAAATCGTTGCCGAGCTGGACCGCCACATTGTTGGCCAGAACGCCGCCAAGCGCGCGGT
GGCCATTGCCCTGCGCAACCGGTGGCGGCGCAAGCAGGCCCCGGAAAATCTGCGGGGCGAGATTACGCCGAAGAACATCC
TGATGATCGGGCCGACGGGCGTGGGTAAGACGGAAGTGTCCCGACGCCTCGCGCGACTGGCCAATGCGCCCTTCCTGAAG
GTGGAAGCGACAAAGTTTACTGAAGTTGGCTATGTCGGCCGGGATGTTGAGCAGATCGTGCGCGATCTGGTTGAGGCAGC
CGTCGGCATGATCCGCGAGCAGAAGCGCGCGGGCGTCGACAAGGCTGCACGGGACAAGGCGGAGGAGCGGCTGCTGGACG
CGCTGGTGGGCGCCGAGGCGCAAAGCTCGACCCGTGAAGTGTTCCGCCGCAAGCTGCGCGCGGGCGAGCTGGACGACAAG
GAAGTCGATCTCGACTTTGCCGACACGAACAACCCGATGCAGATGCTAGACCTGCCCGGCCAGGGTGGCTCAATGAGCAT
GATCAATCTGGGCGACATGCTGGGCAAGGCGATGGGCGGGCGCACCCGTCGGGTGCGAACGACAGTGCGCGAAGCCGCCA
AGCCGCTAGTGACCGAGGAAGCCGACAAGCTGGTGGATGAGGAACAGATCGTCCGTGAAGCGATCATGGCCGTGGAAGAA
GATGGCATCGTCTTCCTCGACGAGATCGACAAGATTGCCGCGCGCAAGGACCGGGGCGGCGCGGACGTAAGCCGCGAGGG
CGTGCAGCGCGACCTGCTGCCGCTGATTGAAGGCACGACCGTTTCGACCAAGCGCGGCGCGGTGAAGACCGACCATATTC
TGTTCATTGCGTCTGGCGCGTTCCACGTTGCCAAGCCATCAGACCTGCTGCCCGAGCTTCAGGGCCGCCTGCCGATCCGC
GTGGAGCTGGAGCCGCTGACGCGGGACGATCTGCGCCGCATTCTGGTCGAGCCGCAGGCCAGCCTGATCCGTCAGTATGA
GGCACTTATGGCGGCGGAGAATGTCACGCTGACCTTCGAGGACGGTGCCATCGACCGGATCGCGGATATGGCCGAGGCGG
TAAACAAGTCCATCGAGAATATCGGCGCGCGGCGTTTGCAGACCATTCTGGAGCGGCTGCTGGATGACATCAGCTTTGAC
GCCCCCGACAAGGGCGGCGAGACCTTCACGATCACGGCAAGTTATGTGGACGAGAAAGTTGGTAGCCTGGCCGGGAATGC
GGATCTGTCGAAGTTTATTCTGTAG

Upstream 100 bases:

>100_bases
CCGGCGCCGGAGCGGATAACGCTCCAAGGGCGGCGCCCATAAGCTCCATTCAGAGCGGCCATCATAAGGCCCCCACCAGA
TACAAGATGAGATCGATTAG

Downstream 100 bases:

>100_bases
CCTGGCGGGCTCAGCTCTCGATTGCCGCCCGCCGGATCGGCAGGTTTTTCTTGCCCCATTCCTTCATGATGGTGAGGGCT
GGGCGGAGTTCCTCGCCCTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKHLTPREIVAELDRHIVGQNAAKRAVAIALRNRWRRKQAPENLRGEITPKNILMIGPTGVGKTEVSRRLARLANAPFLK
VEATKFTEVGYVGRDVEQIVRDLVEAAVGMIREQKRAGVDKAARDKAEERLLDALVGAEAQSSTREVFRRKLRAGELDDK
EVDLDFADTNNPMQMLDLPGQGGSMSMINLGDMLGKAMGGRTRRVRTTVREAAKPLVTEEADKLVDEEQIVREAIMAVEE
DGIVFLDEIDKIAARKDRGGADVSREGVQRDLLPLIEGTTVSTKRGAVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELEPLTRDDLRRILVEPQASLIRQYEALMAAENVTLTFEDGAIDRIADMAEAVNKSIENIGARRLQTILERLLDDISFD
APDKGGETFTITASYVDEKVGSLAGNADLSKFIL

Sequences:

>Translated_434_residues
MKHLTPREIVAELDRHIVGQNAAKRAVAIALRNRWRRKQAPENLRGEITPKNILMIGPTGVGKTEVSRRLARLANAPFLK
VEATKFTEVGYVGRDVEQIVRDLVEAAVGMIREQKRAGVDKAARDKAEERLLDALVGAEAQSSTREVFRRKLRAGELDDK
EVDLDFADTNNPMQMLDLPGQGGSMSMINLGDMLGKAMGGRTRRVRTTVREAAKPLVTEEADKLVDEEQIVREAIMAVEE
DGIVFLDEIDKIAARKDRGGADVSREGVQRDLLPLIEGTTVSTKRGAVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELEPLTRDDLRRILVEPQASLIRQYEALMAAENVTLTFEDGAIDRIADMAEAVNKSIENIGARRLQTILERLLDDISFD
APDKGGETFTITASYVDEKVGSLAGNADLSKFIL
>Mature_434_residues
MKHLTPREIVAELDRHIVGQNAAKRAVAIALRNRWRRKQAPENLRGEITPKNILMIGPTGVGKTEVSRRLARLANAPFLK
VEATKFTEVGYVGRDVEQIVRDLVEAAVGMIREQKRAGVDKAARDKAEERLLDALVGAEAQSSTREVFRRKLRAGELDDK
EVDLDFADTNNPMQMLDLPGQGGSMSMINLGDMLGKAMGGRTRRVRTTVREAAKPLVTEEADKLVDEEQIVREAIMAVEE
DGIVFLDEIDKIAARKDRGGADVSREGVQRDLLPLIEGTTVSTKRGAVKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELEPLTRDDLRRILVEPQASLIRQYEALMAAENVTLTFEDGAIDRIADMAEAVNKSIENIGARRLQTILERLLDDISFD
APDKGGETFTITASYVDEKVGSLAGNADLSKFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=57.6233183856502, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1786642, Length=277, Percent_Identity=32.129963898917, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982905, Length=296, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982908, Length=296, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=36.1702127659575, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648291, Length=303, Percent_Identity=29.3729372937294, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648289, Length=303, Percent_Identity=29.3729372937294, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_HYPNA (Q0C0L1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760732.1
- ProteinModelPortal:   Q0C0L1
- STRING:   Q0C0L1
- GeneID:   4289264
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2034
- NMPDR:   fig|228405.5.peg.1959
- TIGR:   HNE_2034
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- PhylomeDB:   Q0C0L1
- ProtClustDB:   PRK05201
- BioCyc:   HNEP81032:HNE_2034-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 47900; Mature: 47900

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 247-247 BINDING 312-312 BINDING 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLTPREIVAELDRHIVGQNAAKRAVAIALRNRWRRKQAPENLRGEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
VGKTEVSRRLARLANAPFLKVEATKFTEVGYVGRDVEQIVRDLVEAAVGMIREQKRAGVD
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KAARDKAEERLLDALVGAEAQSSTREVFRRKLRAGELDDKEVDLDFADTNNPMQMLDLPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECCC
QGGSMSMINLGDMLGKAMGGRTRRVRTTVREAAKPLVTEEADKLVDEEQIVREAIMAVEE
CCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCC
DGIVFLDEIDKIAARKDRGGADVSREGVQRDLLPLIEGTTVSTKRGAVKTDHILFIASGA
CCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
FHVAKPSDLLPELQGRLPIRVELEPLTRDDLRRILVEPQASLIRQYEALMAAENVTLTFE
EECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEC
DGAIDRIADMAEAVNKSIENIGARRLQTILERLLDDISFDAPDKGGETFTITASYVDEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHH
GSLAGNADLSKFIL
HHHCCCCCHHHHCC
>Mature Secondary Structure
MKHLTPREIVAELDRHIVGQNAAKRAVAIALRNRWRRKQAPENLRGEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCC
VGKTEVSRRLARLANAPFLKVEATKFTEVGYVGRDVEQIVRDLVEAAVGMIREQKRAGVD
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KAARDKAEERLLDALVGAEAQSSTREVFRRKLRAGELDDKEVDLDFADTNNPMQMLDLPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEECCC
QGGSMSMINLGDMLGKAMGGRTRRVRTTVREAAKPLVTEEADKLVDEEQIVREAIMAVEE
CCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCC
DGIVFLDEIDKIAARKDRGGADVSREGVQRDLLPLIEGTTVSTKRGAVKTDHILFIASGA
CCEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
FHVAKPSDLLPELQGRLPIRVELEPLTRDDLRRILVEPQASLIRQYEALMAAENVTLTFE
EECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEC
DGAIDRIADMAEAVNKSIENIGARRLQTILERLLDDISFDAPDKGGETFTITASYVDEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHH
GSLAGNADLSKFIL
HHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA