Click here to switch to the map view.

The map label for this gene is thrA [C]

Identifier: 114800224

GI number: 114800224

Start: 1679469

End: 1680917

Strand: Direct

Name: thrA [C]

Synonym: HNE_1643

Alternate gene names: 114800224

Gene position: 1679469-1680917 (Clockwise)

Preceding gene: 114798545

Following gene: 114798292

Centisome position: 45.33

GC content: 60.59

Gene sequence:

>1449_bases
ATGAAACGCACAGTCGAAAAGATCGGGGGCACCTCGATCTCCAACACGCAGGTCGTGTTGGAAAACGTGCTGATCGGAGG
CCGCAAGGGCGCCGATCTCTATAACCGCATCTTCGTTGTGTCGGCCTATTCTGGCATGACGAACCGCCTGCTGGAACACA
AGAAATCCGGCGAGCCGGGCGTGTTCAGCCTGTTTGCCGGTGCAGGCAACAAATGGGCCTGGACCGAGGCGCTCGCCTCT
GTCGCCGAAGCCATGGAAGCCAAGAACGCTGAAATGTTCTCTGGTGAGCCCGAACAGGCCGTCGCGGACAATTTTGTGCG
GGAACGGATCGAAGACGTTCGCAACTGCCTGATCGATCTCCACCGCCTCTGTTCCTACGGCCAATTCCGTCTGGACGAAA
ACCTCACCACCGTGAAGGAAGTCCTCTCTTCCCTCGGCGAGGCGCACTCCGCCTTCAACACGGCGCTTCTCCTGCAGAAA
CACGGCGTGAACGCTCGCGCTGTCGATCTGACGGCATGGCGCGACGAGCGGGACCTGACCATGGAAGAGCGTATGGAAGA
TGCCCTCGGCGGCATTGACCTGTCCAGCGAACTTCCCATCGTCACCGGCTATGCCAGCAGCCGCGACGGCCTCGTCCGTC
AGTTCGGCCGGGGGTACACAGAGGTTACATTCTCGCGTTTGGCCGCCTTCGTCCAAGCCGATGAAGCGATCATTCACAAG
GAGTTCCACCTCTCCAGCGCCGACCCCAATCTCGTGGGTCAGGACCGGGTCCGCAAGATCGGCCTGACCAACTTTGATGT
GGCCGACCAGCTCTCCAATATGGGCATGGAAGCGGTCCACCCCAAAGCCGCCAAGATCCTGCGCCAGGCGGGCATCCCCC
TGCGCGTCAAGAACACCTTCGACCCTAAGGATGGCGGCACCGTCATCTCGGCAGACTACGCCCCGGAAAACCCCCGCGCC
GAAATCGTCACGGGCCTAAAGGGTGTCTTCGCGCTAGAAGTCTTCGATCAGGACATGGTCGGTGAGAAGGGCTACGATTC
CACCATCCTCGATGCCCTCACGCGCCATTCCATCCGTATCGTGTCGAAATGCTCGAATGCCAACACGATTACCCACTATA
TCGAGGGCAGCCGCAAAGCACTCAAGCGTGCCACCGCGGACATCGAGACAAAGCTCCCCGGCGCGGAAGTCTCCACCAGC
CGCGTTGCCATCGTCTCGGTCATCGGCGCAGACATCAACGTGCCCGGCATCACGGCCAAAGCGCTCACAGCCCTGCATGA
AGCTGAAGTGCCGATCATCGGTCTGCATCAGGTTTCACGGAAAACCGACATTCAGGCCGTCATCCAGGAAGATGATTTCG
ACAAGGCCATCTGCGCCCTGCACGAAGCGCTGGTCGAACAGGTAAGCGTTGCCACGCCGCGCGCTGAGGCCTTGCGCCCG
GCGGCATGA

Upstream 100 bases:

>100_bases
GAGACTAGGCCGCGTCCGGAATTTGTCGCCCACCGTCAGGTGAAGGAGCCGATCCGGCCCCTCAAGGCGCCGCAATCGCG
AACAACCGGGGGTCAATAAC

Downstream 100 bases:

>100_bases
TCCGATTGCTTGGCAGGCCGGGGCTGGTCCTGTCCGGACACGGCGGATTTGCGGTCTACCGGAACATTCTTGTTCTGGTG
GCCGCACTGACGCTCCTTCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKRTVEKIGGTSISNTQVVLENVLIGGRKGADLYNRIFVVSAYSGMTNRLLEHKKSGEPGVFSLFAGAGNKWAWTEALAS
VAEAMEAKNAEMFSGEPEQAVADNFVRERIEDVRNCLIDLHRLCSYGQFRLDENLTTVKEVLSSLGEAHSAFNTALLLQK
HGVNARAVDLTAWRDERDLTMEERMEDALGGIDLSSELPIVTGYASSRDGLVRQFGRGYTEVTFSRLAAFVQADEAIIHK
EFHLSSADPNLVGQDRVRKIGLTNFDVADQLSNMGMEAVHPKAAKILRQAGIPLRVKNTFDPKDGGTVISADYAPENPRA
EIVTGLKGVFALEVFDQDMVGEKGYDSTILDALTRHSIRIVSKCSNANTITHYIEGSRKALKRATADIETKLPGAEVSTS
RVAIVSVIGADINVPGITAKALTALHEAEVPIIGLHQVSRKTDIQAVIQEDDFDKAICALHEALVEQVSVATPRAEALRP
AA

Sequences:

>Translated_482_residues
MKRTVEKIGGTSISNTQVVLENVLIGGRKGADLYNRIFVVSAYSGMTNRLLEHKKSGEPGVFSLFAGAGNKWAWTEALAS
VAEAMEAKNAEMFSGEPEQAVADNFVRERIEDVRNCLIDLHRLCSYGQFRLDENLTTVKEVLSSLGEAHSAFNTALLLQK
HGVNARAVDLTAWRDERDLTMEERMEDALGGIDLSSELPIVTGYASSRDGLVRQFGRGYTEVTFSRLAAFVQADEAIIHK
EFHLSSADPNLVGQDRVRKIGLTNFDVADQLSNMGMEAVHPKAAKILRQAGIPLRVKNTFDPKDGGTVISADYAPENPRA
EIVTGLKGVFALEVFDQDMVGEKGYDSTILDALTRHSIRIVSKCSNANTITHYIEGSRKALKRATADIETKLPGAEVSTS
RVAIVSVIGADINVPGITAKALTALHEAEVPIIGLHQVSRKTDIQAVIQEDDFDKAICALHEALVEQVSVATPRAEALRP
AA
>Mature_482_residues
MKRTVEKIGGTSISNTQVVLENVLIGGRKGADLYNRIFVVSAYSGMTNRLLEHKKSGEPGVFSLFAGAGNKWAWTEALAS
VAEAMEAKNAEMFSGEPEQAVADNFVRERIEDVRNCLIDLHRLCSYGQFRLDENLTTVKEVLSSLGEAHSAFNTALLLQK
HGVNARAVDLTAWRDERDLTMEERMEDALGGIDLSSELPIVTGYASSRDGLVRQFGRGYTEVTFSRLAAFVQADEAIIHK
EFHLSSADPNLVGQDRVRKIGLTNFDVADQLSNMGMEAVHPKAAKILRQAGIPLRVKNTFDPKDGGTVISADYAPENPRA
EIVTGLKGVFALEVFDQDMVGEKGYDSTILDALTRHSIRIVSKCSNANTITHYIEGSRKALKRATADIETKLPGAEVSTS
RVAIVSVIGADINVPGITAKALTALHEAEVPIIGLHQVSRKTDIQAVIQEDDFDKAICALHEALVEQVSVATPRAEALRP
AA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=496, Percent_Identity=24.1935483870968, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=368, Percent_Identity=23.6413043478261, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52394; Mature: 52394

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTVEKIGGTSISNTQVVLENVLIGGRKGADLYNRIFVVSAYSGMTNRLLEHKKSGEPG
CCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHCCCCCC
VFSLFAGAGNKWAWTEALASVAEAMEAKNAEMFSGEPEQAVADNFVRERIEDVRNCLIDL
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HRLCSYGQFRLDENLTTVKEVLSSLGEAHSAFNTALLLQKHGVNARAVDLTAWRDERDLT
HHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
MEERMEDALGGIDLSSELPIVTGYASSRDGLVRQFGRGYTEVTFSRLAAFVQADEAIIHK
HHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EFHLSSADPNLVGQDRVRKIGLTNFDVADQLSNMGMEAVHPKAAKILRQAGIPLRVKNTF
HHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHCCHHHHHHHHHCCCCEEECCCC
DPKDGGTVISADYAPENPRAEIVTGLKGVFALEVFDQDMVGEKGYDSTILDALTRHSIRI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHH
VSKCSNANTITHYIEGSRKALKRATADIETKLPGAEVSTSRVAIVSVIGADINVPGITAK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ALTALHEAEVPIIGLHQVSRKTDIQAVIQEDDFDKAICALHEALVEQVSVATPRAEALRP
HHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCC
AA
CC
>Mature Secondary Structure
MKRTVEKIGGTSISNTQVVLENVLIGGRKGADLYNRIFVVSAYSGMTNRLLEHKKSGEPG
CCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHEEEEEEECCHHHHHHHHHHCCCCCC
VFSLFAGAGNKWAWTEALASVAEAMEAKNAEMFSGEPEQAVADNFVRERIEDVRNCLIDL
EEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HRLCSYGQFRLDENLTTVKEVLSSLGEAHSAFNTALLLQKHGVNARAVDLTAWRDERDLT
HHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
MEERMEDALGGIDLSSELPIVTGYASSRDGLVRQFGRGYTEVTFSRLAAFVQADEAIIHK
HHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EFHLSSADPNLVGQDRVRKIGLTNFDVADQLSNMGMEAVHPKAAKILRQAGIPLRVKNTF
HHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCHHCCHHHHHHHHHCCCCEEECCCC
DPKDGGTVISADYAPENPRAEIVTGLKGVFALEVFDQDMVGEKGYDSTILDALTRHSIRI
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHH
VSKCSNANTITHYIEGSRKALKRATADIETKLPGAEVSTSRVAIVSVIGADINVPGITAK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ALTALHEAEVPIIGLHQVSRKTDIQAVIQEDDFDKAICALHEALVEQVSVATPRAEALRP
HHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]