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The map label for this gene is rsmE [H]

Identifier: 114800139

GI number: 114800139

Start: 1399220

End: 1399990

Strand: Direct

Name: rsmE [H]

Synonym: HNE_1369

Alternate gene names: 114800139

Gene position: 1399220-1399990 (Clockwise)

Preceding gene: 114797958

Following gene: 114799792

Centisome position: 37.77

GC content: 68.87

Gene sequence:

>771_bases
ATGGCTACGATTCCCCGTCTCTTTGTGGATGCGCCGCTGGCGGAAGCTGGCGCAGTTGCACTGGATGACGAGCAGTCGAA
CTATCTGCTGCGGGTTCTGAGGCTGGAGGATGGCGCGTCTGTGCGCCTCTTCAACGGGCGGGACGGGGAGTGGACGGCGA
CGGTCCATAAGACGTCAGCCAAGCGCGCGGAAGCAACACCCTCGAGACAAACCCGGACACAACCAGGACAAATCGGGACA
CGTCTGACCCTCCTGTTTGCGCCGGTGAAGAAGGACCAGACCGACCTCATCCTCGAGAAGGCGACCGAGCTGGGCGCCGC
CAGGATCCGCCCCGTGCTGACCGAGCGGACCCAGACGCGGACCGTGCGGGCCGACCGGTTCCGCAAGATTGCGCTGGAGG
CGGCTGAGCAGACCGAGCGGCTGGACCTGCCGGAGATCCTCGACCTGGCAAGCCTCGACGCGGCGCTGGACGCGCTGGAG
CCGGGCACGGCGGTGATCTTCTGCGATGAGGCGGGCGACGAGGCCGAGGCGCCCTGGGGCGGGCAGGCAGGCCGGGCGCG
GCCGATGCTGGAGGTATTGGAGGAGCTGAAGGGCCGATCCGCCGCAATCCTGACAGGGCCGGAGGGCGGCTTCACCCCGG
CCGAGCGCGCGTTCCTGCGCGGGCGGGCGGAGACCTGGCCGGTGAGCCTTGGGCCGCGCATCCTGCGAGCGGAGACGGCG
GCGATTTCAGCGATGACGCTGTGGCAGGCGGTGTGTGGAGATTGGGGCTAG

Upstream 100 bases:

>100_bases
GATCCCGCAGCCACTCGCACGGGGTGTGATTTCAAAACCACAGGACGTTGCGCAAAGGCCACACCTTAACAGCGGCGGCC
TGGCGGCTTATAGGCCGGAG

Downstream 100 bases:

>100_bases
GCGGGTAGGCAGACACCGGCCCGCCACCCTTGAAGTGGCGCAGGATGGCGGGAGTTGGTGATCTCAATCAGGTGTCGGAT
GTATTGAACGTGGCGGCGGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MATIPRLFVDAPLAEAGAVALDDEQSNYLLRVLRLEDGASVRLFNGRDGEWTATVHKTSAKRAEATPSRQTRTQPGQIGT
RLTLLFAPVKKDQTDLILEKATELGAARIRPVLTERTQTRTVRADRFRKIALEAAEQTERLDLPEILDLASLDAALDALE
PGTAVIFCDEAGDEAEAPWGGQAGRARPMLEVLEELKGRSAAILTGPEGGFTPAERAFLRGRAETWPVSLGPRILRAETA
AISAMTLWQAVCGDWG

Sequences:

>Translated_256_residues
MATIPRLFVDAPLAEAGAVALDDEQSNYLLRVLRLEDGASVRLFNGRDGEWTATVHKTSAKRAEATPSRQTRTQPGQIGT
RLTLLFAPVKKDQTDLILEKATELGAARIRPVLTERTQTRTVRADRFRKIALEAAEQTERLDLPEILDLASLDAALDALE
PGTAVIFCDEAGDEAEAPWGGQAGRARPMLEVLEELKGRSAAILTGPEGGFTPAERAFLRGRAETWPVSLGPRILRAETA
AISAMTLWQAVCGDWG
>Mature_255_residues
ATIPRLFVDAPLAEAGAVALDDEQSNYLLRVLRLEDGASVRLFNGRDGEWTATVHKTSAKRAEATPSRQTRTQPGQIGTR
LTLLFAPVKKDQTDLILEKATELGAARIRPVLTERTQTRTVRADRFRKIALEAAEQTERLDLPEILDLASLDAALDALEP
GTAVIFCDEAGDEAEAPWGGQAGRARPMLEVLEELKGRSAAILTGPEGGFTPAERAFLRGRAETWPVSLGPRILRAETAA
ISAMTLWQAVCGDWG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=264, Percent_Identity=28.030303030303, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27782; Mature: 27651

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIPRLFVDAPLAEAGAVALDDEQSNYLLRVLRLEDGASVRLFNGRDGEWTATVHKTSA
CCCCCCHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEEECCCC
KRAEATPSRQTRTQPGQIGTRLTLLFAPVKKDQTDLILEKATELGAARIRPVLTERTQTR
HHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
TVRADRFRKIALEAAEQTERLDLPEILDLASLDAALDALEPGTAVIFCDEAGDEAEAPWG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GQAGRARPMLEVLEELKGRSAAILTGPEGGFTPAERAFLRGRAETWPVSLGPRILRAETA
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AISAMTLWQAVCGDWG
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATIPRLFVDAPLAEAGAVALDDEQSNYLLRVLRLEDGASVRLFNGRDGEWTATVHKTSA
CCCCCHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEEECCCC
KRAEATPSRQTRTQPGQIGTRLTLLFAPVKKDQTDLILEKATELGAARIRPVLTERTQTR
HHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
TVRADRFRKIALEAAEQTERLDLPEILDLASLDAALDALEPGTAVIFCDEAGDEAEAPWG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GQAGRARPMLEVLEELKGRSAAILTGPEGGFTPAERAFLRGRAETWPVSLGPRILRAETA
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AISAMTLWQAVCGDWG
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]