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The map label for this gene is gcvA [H]

Identifier: 114800130

GI number: 114800130

Start: 1485031

End: 1485963

Strand: Direct

Name: gcvA [H]

Synonym: HNE_1459

Alternate gene names: 114800130

Gene position: 1485031-1485963 (Clockwise)

Preceding gene: 114797069

Following gene: 114798994

Centisome position: 40.08

GC content: 69.02

Gene sequence:

>933_bases
ATGCTGCCGTCTGATGAACCTGATTCCCGCCCCGGACGCCCCCTGCCGCTGAACGCCCTGCGGGCCTTCGAAGCGGCCGC
GCGGCACCTGTCGATCAAGGCGGCCGCGGGCGAGCTGGGCGTGACCCCATCCGCCGTGTCCCACCAGCTCAGAGGGCTTG
AAGAGGCGCTCGGCGTTGACCTGATGCGACGGTCCGGGCCCGCCCTTGTCCTGACGCCCGCCGGCGCTGCGCTTGCGCCG
TCCCTGACCGAGGGATTTGATAAAATCAGCGACGCGGTGGCTCAGCTGGATCAGACCCGCCGGACCGGTCCTCTGCGCCT
CTCTTTGCTGCCGACTTTCGCCGCCCACTGGCTCTCTCCGAGGCTGGCGGCCTGGCCCTTCGGGCGGGAGGGGTTTGACC
TGCTCATATCGGGGAGCCAGGCCCTGGCAGATCTGACCGCCGGGGAAGCGGATGTGGCTGTGCGCCACGGCGCAGGGCGA
TGGCGCGGCCTGGTGTCAGACCTCCTCTTCCGGGAAACCCTGACCCTTGTCGGGACGCCGGGCCCGGAAGGCGAGATGGC
GCGGGACCGTGTTGCGCGCTCGAATCTATTTCTGTCCGCACACAGACACGATGAGTTTGCGCGGTGGAATGCCACGCTGC
CAGGTGGGCCCGTAGCGCCCCGAACGGTCACGCGTGTGGATTCAACCGGACTGGCGCTGCAGGCAGCCGCTGGCGACGCC
GGGCTCGCGCTGGCAGGCTTGGAAATGGCCGACGTCGATCTTGCGGCTGGCCGGCTGGTGACGCTCTTTGATCATTGTGC
CGACGCGGGCAAAGCCTACTGGCTTGTCTACCCGGACGCGCTGGCGCGGGACCGGCGGGTCCGCAACCTGCGACGCTGGC
TGTTCGAAAATCTCTCTCCGAGGGGACCGGCGCCGGTGTCGCCGGCGTCCTGA

Upstream 100 bases:

>100_bases
TTCTTTTGGGCCAGAGTGGCTATGGCGCGTCGGGCCAGAAGCGCTTATCGTGGACAGGACCCGGTCGCCCCTGTCCGGGG
CCGTTGCCTGATAAAGACCG

Downstream 100 bases:

>100_bases
GGCGGGCGTCAGAGCCCCTGAACCAGAAGGACGGGGCCGGACGCCGAGGTCAGACGGTCCTGGGCGATCGCTTTATAATC
TTCCGAGTTCGCCCAGCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLPSDEPDSRPGRPLPLNALRAFEAAARHLSIKAAAGELGVTPSAVSHQLRGLEEALGVDLMRRSGPALVLTPAGAALAP
SLTEGFDKISDAVAQLDQTRRTGPLRLSLLPTFAAHWLSPRLAAWPFGREGFDLLISGSQALADLTAGEADVAVRHGAGR
WRGLVSDLLFRETLTLVGTPGPEGEMARDRVARSNLFLSAHRHDEFARWNATLPGGPVAPRTVTRVDSTGLALQAAAGDA
GLALAGLEMADVDLAAGRLVTLFDHCADAGKAYWLVYPDALARDRRVRNLRRWLFENLSPRGPAPVSPAS

Sequences:

>Translated_310_residues
MLPSDEPDSRPGRPLPLNALRAFEAAARHLSIKAAAGELGVTPSAVSHQLRGLEEALGVDLMRRSGPALVLTPAGAALAP
SLTEGFDKISDAVAQLDQTRRTGPLRLSLLPTFAAHWLSPRLAAWPFGREGFDLLISGSQALADLTAGEADVAVRHGAGR
WRGLVSDLLFRETLTLVGTPGPEGEMARDRVARSNLFLSAHRHDEFARWNATLPGGPVAPRTVTRVDSTGLALQAAAGDA
GLALAGLEMADVDLAAGRLVTLFDHCADAGKAYWLVYPDALARDRRVRNLRRWLFENLSPRGPAPVSPAS
>Mature_310_residues
MLPSDEPDSRPGRPLPLNALRAFEAAARHLSIKAAAGELGVTPSAVSHQLRGLEEALGVDLMRRSGPALVLTPAGAALAP
SLTEGFDKISDAVAQLDQTRRTGPLRLSLLPTFAAHWLSPRLAAWPFGREGFDLLISGSQALADLTAGEADVAVRHGAGR
WRGLVSDLLFRETLTLVGTPGPEGEMARDRVARSNLFLSAHRHDEFARWNATLPGGPVAPRTVTRVDSTGLALQAAAGDA
GLALAGLEMADVDLAAGRLVTLFDHCADAGKAYWLVYPDALARDRRVRNLRRWLFENLSPRGPAPVSPAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=35.6401384083045, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=29.7945205479452, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788706, Length=286, Percent_Identity=26.9230769230769, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=26.7796610169492, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32954; Mature: 32954

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSDEPDSRPGRPLPLNALRAFEAAARHLSIKAAAGELGVTPSAVSHQLRGLEEALGVD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LMRRSGPALVLTPAGAALAPSLTEGFDKISDAVAQLDQTRRTGPLRLSLLPTFAAHWLSP
HHHCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCC
RLAAWPFGREGFDLLISGSQALADLTAGEADVAVRHGAGRWRGLVSDLLFRETLTLVGTP
CHHCCCCCCCCCEEEEECCHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEECC
GPEGEMARDRVARSNLFLSAHRHDEFARWNATLPGGPVAPRTVTRVDSTGLALQAAAGDA
CCCCHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
GLALAGLEMADVDLAAGRLVTLFDHCADAGKAYWLVYPDALARDRRVRNLRRWLFENLSP
CEEEECCCHHCCHHHCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
RGPAPVSPAS
CCCCCCCCCC
>Mature Secondary Structure
MLPSDEPDSRPGRPLPLNALRAFEAAARHLSIKAAAGELGVTPSAVSHQLRGLEEALGVD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
LMRRSGPALVLTPAGAALAPSLTEGFDKISDAVAQLDQTRRTGPLRLSLLPTFAAHWLSP
HHHCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCC
RLAAWPFGREGFDLLISGSQALADLTAGEADVAVRHGAGRWRGLVSDLLFRETLTLVGTP
CHHCCCCCCCCCEEEEECCHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEECC
GPEGEMARDRVARSNLFLSAHRHDEFARWNATLPGGPVAPRTVTRVDSTGLALQAAAGDA
CCCCHHHHHHHHHHHEEEEECCCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
GLALAGLEMADVDLAAGRLVTLFDHCADAGKAYWLVYPDALARDRRVRNLRRWLFENLSP
CEEEECCCHHCCHHHCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCC
RGPAPVSPAS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]