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The map label for this gene is amiD [H]

Identifier: 114800083

GI number: 114800083

Start: 473062

End: 473796

Strand: Reverse

Name: amiD [H]

Synonym: HNE_0480

Alternate gene names: 114800083

Gene position: 473796-473062 (Counterclockwise)

Preceding gene: 114798186

Following gene: 114798469

Centisome position: 12.79

GC content: 67.76

Gene sequence:

>735_bases
ATGGAAATCGAAGACACCCCCAGCCCGAACTTTGACGACCGCAAACACCCCGTCACCCTGCTCGTGCTCCATTATACGGG
CATGGAAAGCGGCGAGGCCGCCCTCACCCGCATGCGCGATCCAGAGGCGAAGGTGTCGGCCCATTACATGGTCTGGGAAG
ACGGCCGCATCGCCCGCCTCGTGGCTGAAAATGACCGCGCCTGGCATGCCGGTGTCTCGACATGGCAGAGCCTTGAGGAT
TTGAATTCCCGCTCTGTCGGCATCGAGATCGTCAATGGCGGCCATGACTACCCCGCCCCCGGCGGCGCGCTCCCGCCCTA
TCCCGACGCGCAGATCACCGCCGTCATCGCGCTCGCCCGCGAGATCATGGCCCATCACGACATCCCGGCCACCGGCCTCG
TCGCCCATTCGGATATTGCGCCCGCCCGCAAGATCGACCCCGGCGAACATTTCCCCTGGCAGCGCCTGGCAGAGGCCGGC
CTCGGCCTCTGGCCCCCCGTCGAGACAGGCAGCGCCTGCGTCTGGTCAGGCGGCGACGCATCGCGCCTCAACGCCCATCT
CGCCCAGATCGGTTACGACACCTCCGACATCCCCGCCGCCCTGCGCGCCTTCCAGCGCCGCTGGATGCCCACCGCCATTA
CCGGCCTTGCAGATGCAGACACCCTGCGCCGCCTCGCCCAGATCGCCGAGGCCTACGCGTTAGGCCCCGTCGGCTCGTCC
TCGCCCCAGGCATGA

Upstream 100 bases:

>100_bases
CCATCGTTTCGCTGTCGGGCGTGCTTGTCATCGCGCTCCGCCCGAACGAGAAAATGCCTGAAGCGGCCCTCGGCAAGAAG
GCCGGCGCCGGAGACTCGTG

Downstream 100 bases:

>100_bases
GCCCACTCGGTCGCCAGCGCCGCCCCCAGCAGCAATGACGACACCGACGCCGACAGCCAGATAAAGCCGAAAATCAGCGA
CGCAATCGCGCCATAGATCG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MEIEDTPSPNFDDRKHPVTLLVLHYTGMESGEAALTRMRDPEAKVSAHYMVWEDGRIARLVAENDRAWHAGVSTWQSLED
LNSRSVGIEIVNGGHDYPAPGGALPPYPDAQITAVIALAREIMAHHDIPATGLVAHSDIAPARKIDPGEHFPWQRLAEAG
LGLWPPVETGSACVWSGGDASRLNAHLAQIGYDTSDIPAALRAFQRRWMPTAITGLADADTLRRLAQIAEAYALGPVGSS
SPQA

Sequences:

>Translated_244_residues
MEIEDTPSPNFDDRKHPVTLLVLHYTGMESGEAALTRMRDPEAKVSAHYMVWEDGRIARLVAENDRAWHAGVSTWQSLED
LNSRSVGIEIVNGGHDYPAPGGALPPYPDAQITAVIALAREIMAHHDIPATGLVAHSDIAPARKIDPGEHFPWQRLAEAG
LGLWPPVETGSACVWSGGDASRLNAHLAQIGYDTSDIPAALRAFQRRWMPTAITGLADADTLRRLAQIAEAYALGPVGSS
SPQA
>Mature_244_residues
MEIEDTPSPNFDDRKHPVTLLVLHYTGMESGEAALTRMRDPEAKVSAHYMVWEDGRIARLVAENDRAWHAGVSTWQSLED
LNSRSVGIEIVNGGHDYPAPGGALPPYPDAQITAVIALAREIMAHHDIPATGLVAHSDIAPARKIDPGEHFPWQRLAEAG
LGLWPPVETGSACVWSGGDASRLNAHLAQIGYDTSDIPAALRAFQRRWMPTAITGLADADTLRRLAQIAEAYALGPVGSS
SPQA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=235, Percent_Identity=37.4468085106383, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786300, Length=170, Percent_Identity=37.0588235294118, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26320; Mature: 26320

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEDTPSPNFDDRKHPVTLLVLHYTGMESGEAALTRMRDPEAKVSAHYMVWEDGRIARL
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEE
VAENDRAWHAGVSTWQSLEDLNSRSVGIEIVNGGHDYPAPGGALPPYPDAQITAVIALAR
EECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
EIMAHHDIPATGLVAHSDIAPARKIDPGEHFPWQRLAEAGLGLWPPVETGSACVWSGGDA
HHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCCCH
SRLNAHLAQIGYDTSDIPAALRAFQRRWMPTAITGLADADTLRRLAQIAEAYALGPVGSS
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
SPQA
CCCC
>Mature Secondary Structure
MEIEDTPSPNFDDRKHPVTLLVLHYTGMESGEAALTRMRDPEAKVSAHYMVWEDGRIARL
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCEEEE
VAENDRAWHAGVSTWQSLEDLNSRSVGIEIVNGGHDYPAPGGALPPYPDAQITAVIALAR
EECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
EIMAHHDIPATGLVAHSDIAPARKIDPGEHFPWQRLAEAGLGLWPPVETGSACVWSGGDA
HHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCCCH
SRLNAHLAQIGYDTSDIPAALRAFQRRWMPTAITGLADADTLRRLAQIAEAYALGPVGSS
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
SPQA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]