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The map label for this gene is iaaA [H]

Identifier: 114800051

GI number: 114800051

Start: 3478012

End: 3478884

Strand: Direct

Name: iaaA [H]

Synonym: HNE_3324

Alternate gene names: 114800051

Gene position: 3478012-3478884 (Clockwise)

Preceding gene: 114798065

Following gene: 114797073

Centisome position: 93.87

GC content: 67.24

Gene sequence:

>873_bases
ATGTCAAAAATCTGGGCCATCGCCATCCATGGCGGCGCCGGACCGATCCCGGGCCGCGATTATGCGCCCGAAGAAGCCCA
CATCGCGGGCCTCCTGAAACGCGGCGCAGACGCGCTCGCGAGCGGCGAAGCCGCGCTCGATGTCGTCGAAGCGATGGTCA
TCGATCTGGAAGCCTCCGGTCTCTACGTGGCCGGCAAAGGCGCGAGCCCCAATACCGCCGGAGACTGGGAACTCGACGCC
GCCATCATGGACGGGCGCACCCGCAACGCCGGCGCCGTCGGCGCCCTGCAGGGCTTTGAAAGCCCCATCCGCGCCGCCCG
GCTGGTGATGGAAAAGACACCCAACGTCCTGATCGTCGGCAAAGGCGCAGAGGCCATTGCCAGCGCCGCTGGTCTCGCCC
GCGTCACCGATCCCGCCAGCTATTATGGCGCCGTCGATGAAGACACCGTCCAGGATGGCAGCGTCCAGATGGGCACCGTC
GGCGCCGTCGCGCTCGACATCTATGGCGACATGGCGGCCGCCACCTCCACCGGCGGCGTCCTCTCCAAAACACCCGGCCG
TATCGGCGACACGCCTATCATCGGCGCAGGCACCTGGGCCGACGAGCGCTGCGCCATCTCCTGCACAGGCCTCGGCGAAT
ATTTCATCCGCGCCAACGCCGCCGCAGACGTCTCCGCCCGCATCCGCTATGGCGGCGATACGCTCGGCCCCGCCCTCAGA
GGCGCGCTGGACGATGTCAAACGCCTGGGCGGCAATGGCGGCATGATCGGCGTCTCGGCAACCGGCGAACTCGCCTCCGG
TTTCAATTCCTCTGGTCTCAAGCGCGGCCTCGCCGACTGGAAAGGCCGTTTCGAAGTGGCAACCTTCCTATGA

Upstream 100 bases:

>100_bases
CATATGCCTCTCCCTTCAAAGCTTGTTGTTTCACCTTCCAGACGGTAGCCATGCCGCGAGCAGGCGCCAAGCCTTACGCG
AGGGGAAAGGAACCATTGCG

Downstream 100 bases:

>100_bases
CCGTACTTTTCTGGGCTCTTTCTGTCATCATCGTCTTCGCCATCCTGCTCGCGGCGCAGATGCGGATGATCATTTCCATG
GCGCTGCGCCGCGCGCTGGC

Product: putative asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSKIWAIAIHGGAGPIPGRDYAPEEAHIAGLLKRGADALASGEAALDVVEAMVIDLEASGLYVAGKGASPNTAGDWELDA
AIMDGRTRNAGAVGALQGFESPIRAARLVMEKTPNVLIVGKGAEAIASAAGLARVTDPASYYGAVDEDTVQDGSVQMGTV
GAVALDIYGDMAAATSTGGVLSKTPGRIGDTPIIGAGTWADERCAISCTGLGEYFIRANAAADVSARIRYGGDTLGPALR
GALDDVKRLGGNGGMIGVSATGELASGFNSSGLKRGLADWKGRFEVATFL

Sequences:

>Translated_290_residues
MSKIWAIAIHGGAGPIPGRDYAPEEAHIAGLLKRGADALASGEAALDVVEAMVIDLEASGLYVAGKGASPNTAGDWELDA
AIMDGRTRNAGAVGALQGFESPIRAARLVMEKTPNVLIVGKGAEAIASAAGLARVTDPASYYGAVDEDTVQDGSVQMGTV
GAVALDIYGDMAAATSTGGVLSKTPGRIGDTPIIGAGTWADERCAISCTGLGEYFIRANAAADVSARIRYGGDTLGPALR
GALDDVKRLGGNGGMIGVSATGELASGFNSSGLKRGLADWKGRFEVATFL
>Mature_289_residues
SKIWAIAIHGGAGPIPGRDYAPEEAHIAGLLKRGADALASGEAALDVVEAMVIDLEASGLYVAGKGASPNTAGDWELDAA
IMDGRTRNAGAVGALQGFESPIRAARLVMEKTPNVLIVGKGAEAIASAAGLARVTDPASYYGAVDEDTVQDGSVQMGTVG
AVALDIYGDMAAATSTGGVLSKTPGRIGDTPIIGAGTWADERCAISCTGLGEYFIRANAAADVSARIRYGGDTLGPALRG
ALDDVKRLGGNGGMIGVSATGELASGFNSSGLKRGLADWKGRFEVATFL

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=287, Percent_Identity=39.0243902439024, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI145275200, Length=287, Percent_Identity=39.0243902439024, Blast_Score=147, Evalue=8e-36,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=31.5985130111524, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI285002251, Length=203, Percent_Identity=35.4679802955665, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI285002253, Length=203, Percent_Identity=33.4975369458128, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787050, Length=293, Percent_Identity=42.6621160409556, Blast_Score=198, Evalue=3e-52,
Organism=Drosophila melanogaster, GI18921183, Length=296, Percent_Identity=36.8243243243243, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21357893, Length=264, Percent_Identity=31.4393939393939, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 29283; Mature: 29152

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIWAIAIHGGAGPIPGRDYAPEEAHIAGLLKRGADALASGEAALDVVEAMVIDLEASG
CCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCHHHHHCCHHHHHHHHHHHEEEECCC
LYVAGKGASPNTAGDWELDAAIMDGRTRNAGAVGALQGFESPIRAARLVMEKTPNVLIVG
EEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KGAEAIASAAGLARVTDPASYYGAVDEDTVQDGSVQMGTVGAVALDIYGDMAAATSTGGV
CCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCHHHHCCCCCC
LSKTPGRIGDTPIIGAGTWADERCAISCTGLGEYFIRANAAADVSARIRYGGDTLGPALR
CCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCCCCCEEEEEECCCHHHHHHH
GALDDVKRLGGNGGMIGVSATGELASGFNSSGLKRGLADWKGRFEVATFL
HHHHHHHHHCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SKIWAIAIHGGAGPIPGRDYAPEEAHIAGLLKRGADALASGEAALDVVEAMVIDLEASG
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCHHHHHCCHHHHHHHHHHHEEEECCC
LYVAGKGASPNTAGDWELDAAIMDGRTRNAGAVGALQGFESPIRAARLVMEKTPNVLIVG
EEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
KGAEAIASAAGLARVTDPASYYGAVDEDTVQDGSVQMGTVGAVALDIYGDMAAATSTGGV
CCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCHHHHCCCCCC
LSKTPGRIGDTPIIGAGTWADERCAISCTGLGEYFIRANAAADVSARIRYGGDTLGPALR
CCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCCCCCEEEEEECCCHHHHHHH
GALDDVKRLGGNGGMIGVSATGELASGFNSSGLKRGLADWKGRFEVATFL
HHHHHHHHHCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]