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The map label for this gene is amiC [H]

Identifier: 114800021

GI number: 114800021

Start: 682424

End: 683668

Strand: Direct

Name: amiC [H]

Synonym: HNE_0674

Alternate gene names: 114800021

Gene position: 682424-683668 (Clockwise)

Preceding gene: 114797137

Following gene: 114799492

Centisome position: 18.42

GC content: 63.13

Gene sequence:

>1245_bases
TTGGGCACTCAACGCCCGTCTCATACCGCCGAGCTGATGCTGACACGTATTGTTTCAGTCCTGTTAATACTTTGCTTTGG
CGCCGTTTTTGGCGCCCGCGCCGACGTGTCACAAATCCGCGTTGTCGGTGACGGGGCGCCGACCCGAATCACGATCTGGA
CGGACGCGCCGGAAGAGGCAGAAGCCTTTGTCAGCGAAACGGCGGGTGTGCGGCGCATCATTTTCCCGCTTAGGTCGAAC
GGCTATTCGGCTGAGGGTCTCGGCAGCGGCGGGGTCACGGTCTGGCAGCTGAACCCAGGGCGGCTGGAATTCGAACTCGA
TCGCGCCATGTCCGTCGCGCGCGTCCTGCGCCTGCCGCCGACCGGCAGCGAGATGTCCTATCGCATCATCGTCGATCTCG
ACACGGTCTCCGACGCCCGCTTCTCCAGCGTGGCCCGTCGGGATCAGCGCCGCCTTGCCAAGGCAGAAACAGACGCGGCC
AGGGCCGCTGAAAAACAAGCCACGCTCCTCGCCGGCTCTGCCGCCTCAAGCGCGGGCCGCAAAGCACCGCCCAGATCGTC
CAAAGGACATGTCGTTGTCATTGATGCCGGTCATGGCGGGAAAGATCCCGGCGCCATGGCGATCAATGGCGGTAAAGAGA
AGGACGTGACCCTCAAAGCCGCCCTCGCCCTGAGAGACCTTCTCGAGGCAGATGGCCGCTATGTCGTCAAGCTCGTGCGG
GACACCGACGTCTATGTCGATCACGAAGACCGGGTCACCAAGGCGCGCAACTGGAACGCCGAATTGTTTATCTCGCTGCA
TGCTGACGCCGCAGGCTCCAGCGCCGTCTCGGGGGCTTCGGTCTATACCATCTCAGCCCGCGGGGAGGGCCGGATCGACC
GGGAGGCGAGCAAGAACAACTGGGTTATTCCCATCGAGGATGGCACCCCCCAGCGCCTGACCGGCATTCTCTCCGATCTG
GTCAAACGCGAAACCAAAACGCGTTCGGCCGAGTTTGCCGAACTTCTGCTGCCCGAACTGGAACGCGCAGGCCCCGTCTT
GCGCAATACCCATCGCAGCGCCGGGTTCTATGTCCTGCTTGCGCCCGATGTGCCCGCAGTGCTGCTCGAACTTGGCTTCC
TGACGAATTCCGAAGATGCAAAACGCCTCCAGTCCGAGCGGGGCAGAGCGGCGGCGGCCCTCGCCATCAAGCAGGCCATC
GACACCTATTTCGACCGCCAGGATCTCCGCCTCGCGTCCCAATAG

Upstream 100 bases:

>100_bases
AATTGAAAGAGGCAAATTCCTGCGGCGGGCACTGCAACAGCTAATGCGCGGTCAAGGCGGGGTTAATCTTCCTGTGGGTA
TCCACTGAGATGACCGCATA

Downstream 100 bases:

>100_bases
GCGGCAATTGGCGCAGGTCCCCGCAGCGGGCGCCCACTTGTCGCTGCGGGCAGATACTGCAAGGGTTTGGCCCATGAAAC
GCATCCTTCCGCCGCTCCTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGTQRPSHTAELMLTRIVSVLLILCFGAVFGARADVSQIRVVGDGAPTRITIWTDAPEEAEAFVSETAGVRRIIFPLRSN
GYSAEGLGSGGVTVWQLNPGRLEFELDRAMSVARVLRLPPTGSEMSYRIIVDLDTVSDARFSSVARRDQRRLAKAETDAA
RAAEKQATLLAGSAASSAGRKAPPRSSKGHVVVIDAGHGGKDPGAMAINGGKEKDVTLKAALALRDLLEADGRYVVKLVR
DTDVYVDHEDRVTKARNWNAELFISLHADAAGSSAVSGASVYTISARGEGRIDREASKNNWVIPIEDGTPQRLTGILSDL
VKRETKTRSAEFAELLLPELERAGPVLRNTHRSAGFYVLLAPDVPAVLLELGFLTNSEDAKRLQSERGRAAAALAIKQAI
DTYFDRQDLRLASQ

Sequences:

>Translated_414_residues
MGTQRPSHTAELMLTRIVSVLLILCFGAVFGARADVSQIRVVGDGAPTRITIWTDAPEEAEAFVSETAGVRRIIFPLRSN
GYSAEGLGSGGVTVWQLNPGRLEFELDRAMSVARVLRLPPTGSEMSYRIIVDLDTVSDARFSSVARRDQRRLAKAETDAA
RAAEKQATLLAGSAASSAGRKAPPRSSKGHVVVIDAGHGGKDPGAMAINGGKEKDVTLKAALALRDLLEADGRYVVKLVR
DTDVYVDHEDRVTKARNWNAELFISLHADAAGSSAVSGASVYTISARGEGRIDREASKNNWVIPIEDGTPQRLTGILSDL
VKRETKTRSAEFAELLLPELERAGPVLRNTHRSAGFYVLLAPDVPAVLLELGFLTNSEDAKRLQSERGRAAAALAIKQAI
DTYFDRQDLRLASQ
>Mature_413_residues
GTQRPSHTAELMLTRIVSVLLILCFGAVFGARADVSQIRVVGDGAPTRITIWTDAPEEAEAFVSETAGVRRIIFPLRSNG
YSAEGLGSGGVTVWQLNPGRLEFELDRAMSVARVLRLPPTGSEMSYRIIVDLDTVSDARFSSVARRDQRRLAKAETDAAR
AAEKQATLLAGSAASSAGRKAPPRSSKGHVVVIDAGHGGKDPGAMAINGGKEKDVTLKAALALRDLLEADGRYVVKLVRD
TDVYVDHEDRVTKARNWNAELFISLHADAAGSSAVSGASVYTISARGEGRIDREASKNNWVIPIEDGTPQRLTGILSDLV
KRETKTRSAEFAELLLPELERAGPVLRNTHRSAGFYVLLAPDVPAVLLELGFLTNSEDAKRLQSERGRAAAALAIKQAID
TYFDRQDLRLASQ

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=239, Percent_Identity=31.3807531380753, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1790611, Length=240, Percent_Identity=31.6666666666667, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788776, Length=243, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 44665; Mature: 44534

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTQRPSHTAELMLTRIVSVLLILCFGAVFGARADVSQIRVVGDGAPTRITIWTDAPEEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHH
EAFVSETAGVRRIIFPLRSNGYSAEGLGSGGVTVWQLNPGRLEFELDRAMSVARVLRLPP
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCCC
TGSEMSYRIIVDLDTVSDARFSSVARRDQRRLAKAETDAARAAEKQATLLAGSAASSAGR
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KAPPRSSKGHVVVIDAGHGGKDPGAMAINGGKEKDVTLKAALALRDLLEADGRYVVKLVR
CCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEE
DTDVYVDHEDRVTKARNWNAELFISLHADAAGSSAVSGASVYTISARGEGRIDREASKNN
CCCEEECCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
WVIPIEDGTPQRLTGILSDLVKRETKTRSAEFAELLLPELERAGPVLRNTHRSAGFYVLL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
APDVPAVLLELGFLTNSEDAKRLQSERGRAAAALAIKQAIDTYFDRQDLRLASQ
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure 
GTQRPSHTAELMLTRIVSVLLILCFGAVFGARADVSQIRVVGDGAPTRITIWTDAPEEA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCHHH
EAFVSETAGVRRIIFPLRSNGYSAEGLGSGGVTVWQLNPGRLEFELDRAMSVARVLRLPP
HHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHCCCC
TGSEMSYRIIVDLDTVSDARFSSVARRDQRRLAKAETDAARAAEKQATLLAGSAASSAGR
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KAPPRSSKGHVVVIDAGHGGKDPGAMAINGGKEKDVTLKAALALRDLLEADGRYVVKLVR
CCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEE
DTDVYVDHEDRVTKARNWNAELFISLHADAAGSSAVSGASVYTISARGEGRIDREASKNN
CCCEEECCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
WVIPIEDGTPQRLTGILSDLVKRETKTRSAEFAELLLPELERAGPVLRNTHRSAGFYVLL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEE
APDVPAVLLELGFLTNSEDAKRLQSERGRAAAALAIKQAIDTYFDRQDLRLASQ
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]