Click here to switch to the map view.

The map label for this gene is lasT [H]

Identifier: 114799978

GI number: 114799978

Start: 1186488

End: 1187234

Strand: Direct

Name: lasT [H]

Synonym: HNE_1178

Alternate gene names: 114799978

Gene position: 1186488-1187234 (Clockwise)

Preceding gene: 114798736

Following gene: 114797218

Centisome position: 32.02

GC content: 67.47

Gene sequence:

>747_bases
ATGCAACGCGCTCCTGCAATCATTCTTCATGCGCCCCAATTGGGCGAAAATATCGGCGCGGCGGCCCGTGTGATGCTGAA
TTTCGGCCTGACGGACCTGCGGCTGGTGAAGCCGCGCGACGGGTGGCCGAGCGCGCCGGCAGAGGCAATGTCAGCGGGTG
CGCTCGCGCAGGGGACGCAGGTTTCAGTGTTCGAGACGCTGGAAGAGGCGATGACGGGACTGACCTACACCATTGCCACG
ACGGCGCGGCCGCGGGGGATGGAGAAGGCGGTGTTCGGGGCGCCCGACGCTGTGGGTGCGCTCAAGCGCCAGGCGGGGCA
GAGCGCGGTGCTGTTCGGGGGGGAGAAATCGGGCCTGCCGAATGAGGCGGTGGCGCTGTGTGACGCGATCCTGACCTATC
CGGTGAACCCGGACTTTTCCTCGCTGAACCTGGCGCAGGCGGTCGGCGTGTTCTGTCACCAGTGGGGGGCGAGCGCGGGG
CTGGAGGGGAAGTTTGACGGGACCACCGAGGGGCTGGGCGCGCCGGCGCTGCGCGAGGAGCTGATCGGTATGTTCGAGCA
TTTCGAAGCGGAGCTGGAGCGGGCGGGCTATTTCTATCCGCCGGAAAAAACGCCGGTGATGGTGACCAATCTGCGCAACG
CACTGGTGCGCGCGCAGTGGACCCAGCAGGAGGTGCGGACTTTTCGCGGGGCCATCAAGGCGCTGGCGCTGGGGCGCGGG
AAGGCGCGGGTTGTGCGGGAGGAGTGA

Upstream 100 bases:

>100_bases
CCTTGATCTTCGCCATTTTTTGGAATCCTTGCGCTGCATACGGTTGGGCGGGGCTATAGCAGACCCACAGGCCGCGTCAA
AACCCGGAAACGAGGCAGTC

Downstream 100 bases:

>100_bases
GGGGGTGGTCACCGGGTTGTTGAATCGCAGATGGGATTGTGAATTATGCGGCTGTGGGAGCCCGGTCGAGGTGGCGGCGG
AACGACCAAGCTCCACGGAG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQRAPAIILHAPQLGENIGAAARVMLNFGLTDLRLVKPRDGWPSAPAEAMSAGALAQGTQVSVFETLEEAMTGLTYTIAT
TARPRGMEKAVFGAPDAVGALKRQAGQSAVLFGGEKSGLPNEAVALCDAILTYPVNPDFSSLNLAQAVGVFCHQWGASAG
LEGKFDGTTEGLGAPALREELIGMFEHFEAELERAGYFYPPEKTPVMVTNLRNALVRAQWTQQEVRTFRGAIKALALGRG
KARVVREE

Sequences:

>Translated_248_residues
MQRAPAIILHAPQLGENIGAAARVMLNFGLTDLRLVKPRDGWPSAPAEAMSAGALAQGTQVSVFETLEEAMTGLTYTIAT
TARPRGMEKAVFGAPDAVGALKRQAGQSAVLFGGEKSGLPNEAVALCDAILTYPVNPDFSSLNLAQAVGVFCHQWGASAG
LEGKFDGTTEGLGAPALREELIGMFEHFEAELERAGYFYPPEKTPVMVTNLRNALVRAQWTQQEVRTFRGAIKALALGRG
KARVVREE
>Mature_248_residues
MQRAPAIILHAPQLGENIGAAARVMLNFGLTDLRLVKPRDGWPSAPAEAMSAGALAQGTQVSVFETLEEAMTGLTYTIAT
TARPRGMEKAVFGAPDAVGALKRQAGQSAVLFGGEKSGLPNEAVALCDAILTYPVNPDFSSLNLAQAVGVFCHQWGASAG
LEGKFDGTTEGLGAPALREELIGMFEHFEAELERAGYFYPPEKTPVMVTNLRNALVRAQWTQQEVRTFRGAIKALALGRG
KARVVREE

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=183, Percent_Identity=36.6120218579235, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=31.5573770491803, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26454; Mature: 26454

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRAPAIILHAPQLGENIGAAARVMLNFGLTDLRLVKPRDGWPSAPAEAMSAGALAQGTQ
CCCCCEEEEECCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCC
VSVFETLEEAMTGLTYTIATTARPRGMEKAVFGAPDAVGALKRQAGQSAVLFGGEKSGLP
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCC
NEAVALCDAILTYPVNPDFSSLNLAQAVGVFCHQWGASAGLEGKFDGTTEGLGAPALREE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LIGMFEHFEAELERAGYFYPPEKTPVMVTNLRNALVRAQWTQQEVRTFRGAIKALALGRG
HHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KARVVREE
CCEEECCC
>Mature Secondary Structure
MQRAPAIILHAPQLGENIGAAARVMLNFGLTDLRLVKPRDGWPSAPAEAMSAGALAQGTQ
CCCCCEEEEECCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCCCCCCC
VSVFETLEEAMTGLTYTIATTARPRGMEKAVFGAPDAVGALKRQAGQSAVLFGGEKSGLP
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCC
NEAVALCDAILTYPVNPDFSSLNLAQAVGVFCHQWGASAGLEGKFDGTTEGLGAPALREE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
LIGMFEHFEAELERAGYFYPPEKTPVMVTNLRNALVRAQWTQQEVRTFRGAIKALALGRG
HHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KARVVREE
CCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]