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The map label for this gene is merA [H]

Identifier: 114799977

GI number: 114799977

Start: 1731485

End: 1732909

Strand: Direct

Name: merA [H]

Synonym: HNE_1691

Alternate gene names: 114799977

Gene position: 1731485-1732909 (Clockwise)

Preceding gene: 114797310

Following gene: 114799808

Centisome position: 46.73

GC content: 61.4

Gene sequence:

>1425_bases
ATGAAACACGAATGCTGCGCGCCGAACTGTGAGTACGACCTCGCCGTGATTGGTGCCGGATCGGCTGGATTCTCCGCCGC
TATTACGGCCGCAGAGGGCGGTGTGCGCGTGGCTCTTATAGGGCACGGTACGATTGGCGGTACCTGCGTGAACGTCGGAT
GCGTCCCCTCCAAGACGATGATCCGCGCCGCTGAGGCGGTGTTTAGTGCGCAGTCGGCGCATCGGTTCCCGGGACTCAGG
GGAGAAGCGCAGGTTTCTGACTGGGCGGCGCTGATCGCAGCAAAGGAAGATCTCGTCGCGTCACTGCGGCAGAAAAAATA
CGCCAACTTGCTACCAGGCTATCATGGTGTGACTTACATCGATGAGGGGCCAGCGCGGCTCGTACTTGGCGGTGTGGAGG
TCGGCGGACGGAAGATCAAGGTATCCAAGGTCATCGTCGCCACTGGCGGCCGCTCGGCCGTTCCTGACGTCGCCGGTATT
TCGGAGGTGCCGACGCTCGACAGCACGGCGCTCCTGGAGCTGAAGGCGCTGCCCGAGAGCCTGATCTTCCTCGGCGGCGG
CTATATCGGTGTGGAACTCGCACAGATGATGGCGAGGCTGGGTACCCGCGTCACGATCGTGTGCCGCTCGCGGTTGTTGC
CGCGCGCTGAACCAGAGGTGTCCGAGGCGCTCGCGAAGGCACTGCGCGCCGAGGGCATCTCGGTTGTGGAAAAGGTAACC
TATGACGCCGCACGGCTGGACGGCAGCCGCACAGTCGTGACGGTAACGGTGAACGGTGCCGCCAGAGACCTGGCGGCTGA
TCACCTTGTCCTGTCGACTGGGCGCATTCCCAACACGGATGGGCTGGGGCTCAAAGAGATGGGTGTCGAGACTGATCAGC
GGGGCGCGATCATCGTTGGCGATGATATGGCCACCACACGTTCCGGTGTATACGCAGCAGGCGACGTAACTGACCGGGAT
CAGTTCGTATACATGGCCGCTTATGGCGCCAAGCTGGCGGCGCGAAATGCGGTACTGGGAGGGAAGGAATGCTACGACAA
CGCCGCGATGCCGTGGGTGGTTTTCTGCGATCCGCAGATTGCAGGTGTCGGTCTGACCGAGGCGCAGGCTTACGCCGCAG
GCTATGATGTCAAGACCAGTGTGCTTCCGCTTGAGAATGTTCCTCGGGCGCTCGCGGCACGCAATACCACAGGGCTGATC
AAGTTGGTCGCAGACCGGGCTAATGACCGACTGCTCGGCGGTGTGATCATGGCGCAGGAAGGCGCTGATAGTATCCAGAC
ACTTTCACTGGCACTGAAGTTCGGCATGACGACAAAGGCGCTCGGCGAAACCATTTTCCCTTATTTAACGACAGTCGAGG
GGTTCAAGCTGGCGGCGCAGACTTTCGACAAGGATGTCGCGAAACTCTCCTGTTGCGCGGGCTGA

Upstream 100 bases:

>100_bases
CTGGTGATTTCAGGACCGCTACCCGCAATCAATGATTCAAGGGGCCTCCAAGTTTCGCGTCTCTTGCAACTTCACGAGAC
AAGTCTCGAAGGAAAACTCA

Downstream 100 bases:

>100_bases
GTCGCCGATTGGCACGCATTTCCTTTTTGCCGGTCAGTCACACCGCTCCACAAAGCGGCCGGGAGCATCACCGGCATCAA
GCGCGAAGTCTCAGACCGGC

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKHECCAPNCEYDLAVIGAGSAGFSAAITAAEGGVRVALIGHGTIGGTCVNVGCVPSKTMIRAAEAVFSAQSAHRFPGLR
GEAQVSDWAALIAAKEDLVASLRQKKYANLLPGYHGVTYIDEGPARLVLGGVEVGGRKIKVSKVIVATGGRSAVPDVAGI
SEVPTLDSTALLELKALPESLIFLGGGYIGVELAQMMARLGTRVTIVCRSRLLPRAEPEVSEALAKALRAEGISVVEKVT
YDAARLDGSRTVVTVTVNGAARDLAADHLVLSTGRIPNTDGLGLKEMGVETDQRGAIIVGDDMATTRSGVYAAGDVTDRD
QFVYMAAYGAKLAARNAVLGGKECYDNAAMPWVVFCDPQIAGVGLTEAQAYAAGYDVKTSVLPLENVPRALAARNTTGLI
KLVADRANDRLLGGVIMAQEGADSIQTLSLALKFGMTTKALGETIFPYLTTVEGFKLAAQTFDKDVAKLSCCAG

Sequences:

>Translated_474_residues
MKHECCAPNCEYDLAVIGAGSAGFSAAITAAEGGVRVALIGHGTIGGTCVNVGCVPSKTMIRAAEAVFSAQSAHRFPGLR
GEAQVSDWAALIAAKEDLVASLRQKKYANLLPGYHGVTYIDEGPARLVLGGVEVGGRKIKVSKVIVATGGRSAVPDVAGI
SEVPTLDSTALLELKALPESLIFLGGGYIGVELAQMMARLGTRVTIVCRSRLLPRAEPEVSEALAKALRAEGISVVEKVT
YDAARLDGSRTVVTVTVNGAARDLAADHLVLSTGRIPNTDGLGLKEMGVETDQRGAIIVGDDMATTRSGVYAAGDVTDRD
QFVYMAAYGAKLAARNAVLGGKECYDNAAMPWVVFCDPQIAGVGLTEAQAYAAGYDVKTSVLPLENVPRALAARNTTGLI
KLVADRANDRLLGGVIMAQEGADSIQTLSLALKFGMTTKALGETIFPYLTTVEGFKLAAQTFDKDVAKLSCCAG
>Mature_474_residues
MKHECCAPNCEYDLAVIGAGSAGFSAAITAAEGGVRVALIGHGTIGGTCVNVGCVPSKTMIRAAEAVFSAQSAHRFPGLR
GEAQVSDWAALIAAKEDLVASLRQKKYANLLPGYHGVTYIDEGPARLVLGGVEVGGRKIKVSKVIVATGGRSAVPDVAGI
SEVPTLDSTALLELKALPESLIFLGGGYIGVELAQMMARLGTRVTIVCRSRLLPRAEPEVSEALAKALRAEGISVVEKVT
YDAARLDGSRTVVTVTVNGAARDLAADHLVLSTGRIPNTDGLGLKEMGVETDQRGAIIVGDDMATTRSGVYAAGDVTDRD
QFVYMAAYGAKLAARNAVLGGKECYDNAAMPWVVFCDPQIAGVGLTEAQAYAAGYDVKTSVLPLENVPRALAARNTTGLI
KLVADRANDRLLGGVIMAQEGADSIQTLSLALKFGMTTKALGETIFPYLTTVEGFKLAAQTFDKDVAKLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=28.8421052631579, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=29.8924731182796, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI148277071, Length=426, Percent_Identity=26.056338028169, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI148277065, Length=426, Percent_Identity=26.056338028169, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=426, Percent_Identity=26.056338028169, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=426, Percent_Identity=26.056338028169, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=426, Percent_Identity=26.056338028169, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI22035672, Length=477, Percent_Identity=27.6729559748428, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=25.9259259259259, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=24.5901639344262, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI87081717, Length=465, Percent_Identity=32.258064516129, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=29.2631578947368, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789915, Length=436, Percent_Identity=32.1100917431193, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082354, Length=465, Percent_Identity=25.8064516129032, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=30.1075268817204, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=29.6218487394958, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=30.5494505494506, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=30.5494505494506, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=477, Percent_Identity=25.9958071278826, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=30.1229508196721, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=26.3948497854077, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=27.0833333333333, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21358499, Length=478, Percent_Identity=29.0794979079498, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24640551, Length=506, Percent_Identity=29.4466403162055, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640549, Length=502, Percent_Identity=29.0836653386454, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24640553, Length=502, Percent_Identity=29.0836653386454, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17737741, Length=510, Percent_Identity=27.6470588235294, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 49321; Mature: 49321

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHECCAPNCEYDLAVIGAGSAGFSAAITAAEGGVRVALIGHGTIGGTCVNVGCVPSKTM
CCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCHHH
IRAAEAVFSAQSAHRFPGLRGEAQVSDWAALIAAKEDLVASLRQKKYANLLPGYHGVTYI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DEGPARLVLGGVEVGGRKIKVSKVIVATGGRSAVPDVAGISEVPTLDSTALLELKALPES
ECCCCEEEEEEEEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEEHHCCCE
LIFLGGGYIGVELAQMMARLGTRVTIVCRSRLLPRAEPEVSEALAKALRAEGISVVEKVT
EEEECCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
YDAARLDGSRTVVTVTVNGAARDLAADHLVLSTGRIPNTDGLGLKEMGVETDQRGAIIVG
HHHHHCCCCCEEEEEEECCCHHHHHHCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEC
DDMATTRSGVYAAGDVTDRDQFVYMAAYGAKLAARNAVLGGKECYDNAAMPWVVFCDPQI
CCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHCCCCCHHHHCCCCCCEEEEECCCC
AGVGLTEAQAYAAGYDVKTSVLPLENVPRALAARNTTGLIKLVADRANDRLLGGVIMAQE
CCCCCCHHHHHHCCCCCEEEEECHHHCCHHHHHCCCCCEEEEEECCCCCEEEEEEEEECC
GADSIQTLSLALKFGMTTKALGETIFPYLTTVEGFKLAAQTFDKDVAKLSCCAG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHECCAPNCEYDLAVIGAGSAGFSAAITAAEGGVRVALIGHGTIGGTCVNVGCVPSKTM
CCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCHHH
IRAAEAVFSAQSAHRFPGLRGEAQVSDWAALIAAKEDLVASLRQKKYANLLPGYHGVTYI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
DEGPARLVLGGVEVGGRKIKVSKVIVATGGRSAVPDVAGISEVPTLDSTALLELKALPES
ECCCCEEEEEEEEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHEEEHHCCCE
LIFLGGGYIGVELAQMMARLGTRVTIVCRSRLLPRAEPEVSEALAKALRAEGISVVEKVT
EEEECCCHHHHHHHHHHHHHCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
YDAARLDGSRTVVTVTVNGAARDLAADHLVLSTGRIPNTDGLGLKEMGVETDQRGAIIVG
HHHHHCCCCCEEEEEEECCCHHHHHHCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEC
DDMATTRSGVYAAGDVTDRDQFVYMAAYGAKLAARNAVLGGKECYDNAAMPWVVFCDPQI
CCCCCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHCCCCCHHHHCCCCCCEEEEECCCC
AGVGLTEAQAYAAGYDVKTSVLPLENVPRALAARNTTGLIKLVADRANDRLLGGVIMAQE
CCCCCCHHHHHHCCCCCEEEEECHHHCCHHHHHCCCCCEEEEEECCCCCEEEEEEEEECC
GADSIQTLSLALKFGMTTKALGETIFPYLTTVEGFKLAAQTFDKDVAKLSCCAG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]