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The map label for this gene is leuD [H]

Identifier: 114799969

GI number: 114799969

Start: 44200

End: 44829

Strand: Direct

Name: leuD [H]

Synonym: HNE_0052

Alternate gene names: 114799969

Gene position: 44200-44829 (Clockwise)

Preceding gene: 114798233

Following gene: 114798050

Centisome position: 1.19

GC content: 60.79

Gene sequence:

>630_bases
ATGACACCTTTCACACGCCTCGACGGAACCGCCGCGCCGCTGATGGACAAGGGGAAACCCATGTCCAATGTCGATACCGA
CATGATCATCCCCAAACAGTTCCTGAAGACGACCGAGCGGACGGGGCTCGCCAAGGGCCTCTTCTATGAGTTGAAAACGC
GGGCAGACGGTTCAGAAGACCCCGATTTCGTGCTCAACCGGCCGCAATACGCCAAGGCGAGCATTCTCATCGCAGGCGAC
AATTTCGGTTGTGGCTCCTCGCGTGAACATGCGCCGTGGGCCCTGGCCGATCAGGGCATCACCTGCATCATCGCGCCCTC
CTTTGCCGACATCTTCCACAACAATTGCTACAAGAACGGCATTCTTCCCGTGCGCCTGCCAGCCGATGTCTGTGACAAGC
TGGCGGCGGAAGCTGGCGGCCCGAACCATGTCTTCTCGGTGGACCTGCAGGCCCAGACTGTGACGACGCCCTCGGGAGAG
GTGCACGCCTTTGAAGTTGATCCCGGCCGGAAGTCGAATCTGATCGCCGGCCTTGATGAAATTGGCGCGTCCCTCACAGC
CGCGTCCGCCATCGACAATTTCGAGGATCAACGCCGCCTGACAACGCCCTGGCTCGAAGGCGCGCGCTGA

Upstream 100 bases:

>100_bases
AGGGCCGCAAGCCCGGCAGCCGGGTTTTCACGGTCCGGAACGGCACAATGAATGTGCCCACCTTGTTCATTGCCCATGAG
GGGGTTTAAAAGCTCAGCCC

Downstream 100 bases:

>100_bases
ACGTTCGGGCTATCCGTATTCAGGGTCGGCAATTTTATCGACAATCAATTGCAAGGCCCGAACGGCGCTGCCATACATTT
CTGCGGAAACATCTAAATTT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTPFTRLDGTAAPLMDKGKPMSNVDTDMIIPKQFLKTTERTGLAKGLFYELKTRADGSEDPDFVLNRPQYAKASILIAGD
NFGCGSSREHAPWALADQGITCIIAPSFADIFHNNCYKNGILPVRLPADVCDKLAAEAGGPNHVFSVDLQAQTVTTPSGE
VHAFEVDPGRKSNLIAGLDEIGASLTAASAIDNFEDQRRLTTPWLEGAR

Sequences:

>Translated_209_residues
MTPFTRLDGTAAPLMDKGKPMSNVDTDMIIPKQFLKTTERTGLAKGLFYELKTRADGSEDPDFVLNRPQYAKASILIAGD
NFGCGSSREHAPWALADQGITCIIAPSFADIFHNNCYKNGILPVRLPADVCDKLAAEAGGPNHVFSVDLQAQTVTTPSGE
VHAFEVDPGRKSNLIAGLDEIGASLTAASAIDNFEDQRRLTTPWLEGAR
>Mature_208_residues
TPFTRLDGTAAPLMDKGKPMSNVDTDMIIPKQFLKTTERTGLAKGLFYELKTRADGSEDPDFVLNRPQYAKASILIAGDN
FGCGSSREHAPWALADQGITCIIAPSFADIFHNNCYKNGILPVRLPADVCDKLAAEAGGPNHVFSVDLQAQTVTTPSGEV
HAFEVDPGRKSNLIAGLDEIGASLTAASAIDNFEDQRRLTTPWLEGAR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=199, Percent_Identity=46.2311557788945, Blast_Score=161, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321429, Length=217, Percent_Identity=51.6129032258064, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22522; Mature: 22390

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFTRLDGTAAPLMDKGKPMSNVDTDMIIPKQFLKTTERTGLAKGLFYELKTRADGSED
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCHHHHHHHEEHHCCCCCCC
PDFVLNRPQYAKASILIAGDNFGCGSSREHAPWALADQGITCIIAPSFADIFHNNCYKNG
CCEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHCCCHHCC
ILPVRLPADVCDKLAAEAGGPNHVFSVDLQAQTVTTPSGEVHAFEVDPGRKSNLIAGLDE
EEEEEECHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCCCEECHHH
IGASLTAASAIDNFEDQRRLTTPWLEGAR
HCCHHHHHHHHHCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TPFTRLDGTAAPLMDKGKPMSNVDTDMIIPKQFLKTTERTGLAKGLFYELKTRADGSED
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCHHHHHHHEEHHCCCCCCC
PDFVLNRPQYAKASILIAGDNFGCGSSREHAPWALADQGITCIIAPSFADIFHNNCYKNG
CCEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHCCCHHCC
ILPVRLPADVCDKLAAEAGGPNHVFSVDLQAQTVTTPSGEVHAFEVDPGRKSNLIAGLDE
EEEEEECHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCCCEECHHH
IGASLTAASAIDNFEDQRRLTTPWLEGAR
HCCHHHHHHHHHCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA