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The map label for this gene is irlS [H]

Identifier: 114799948

GI number: 114799948

Start: 1343867

End: 1345420

Strand: Reverse

Name: irlS [H]

Synonym: HNE_1311

Alternate gene names: 114799948

Gene position: 1345420-1343867 (Counterclockwise)

Preceding gene: 114797880

Following gene: 114797144

Centisome position: 36.31

GC content: 64.8

Gene sequence:

>1554_bases
ATGAAAACGCCGCTCCAACTTCCCGCATGGCTGGCCCCTCGGGTGCCTGACAGGACCCAGCTCCTGAAACCATTGCGGAT
TCTGGGCAAGGGCATAGGCGCCGGCTGGCGCGGTCTCGGCAAGGCCTGGTCCAGCCTGCCCTTCATGGCGCAGATCAAGA
AGGCGATGCCGGCGTTCGCGCGCACCACCACCTTCAAGCTGACAATCCTCTACTCCGCGATGATTGGCGCCTTCTCCGGC
GCACTGCTCGTTTATCTATATTATTCAACAGTTTACTATATCCGCGCGGAGTCCGAAAACCGCATCACGGTGGAGTTCGA
ACAGCTCGCCAACGCCTATTATACCGGCGGCATGGAACGCCTCAGCCAGTCCGTCTTCGAGCGGATGACGCTCTCCGGCT
CGCAATTCTTCTACTATCTTGAAGACTCCTCCGGCCGGAAGATCGCCGGCCACTTCCCCCGCCTGCCGGCCGATCCCCCT
GCCGCCGGCATGAAGACCGTCTATTTCGATTTCGAGCTGCCCCAGGCCGACGGCACCAACACGCTGCGCCCCGCGGCCGG
CCGCATCGTGCGCCTGCGCGACAATGGCGGCGCGCTGATGGTCGCCTTCGACACCGCCCAGCAGACGGTCATCGTCGGGC
GTATCCGCAACGCCGTTTACGTTGCCATGCCCATCGCGCTCATCCTCTCGCTGGCCGGGGGCCTCGTCATCTCACGCGGC
GCCGCCAAGCGCGCCGATGAACTCGCCAAGACCACCGAAGCCGTCATGGGCGGCGAACTTGGCCGCCGCATGCCCGTGCG
CGGCACAGGCGACGAATTTGACCGCCTCGCCCAACGCCTCAATGCGATGCTCGACCAGATCCAGAAGCTGGTGGAATCCT
CCCGCCATACCGGCAACGCCATCGCCCATGATCTGCGCTCACCGCTCACCCGCCTGCGCAACCGTCTGGAACTTGCGCTC
AGCAAGCCCCTCTCCGAAGAAGCCGCCAACGAAACCCTCGGCACGACGCTCTCGGAAGTCGACCGCGTGCTGGACACCTT
CAACGCCATCCTCCGCCTCGCGCGCCTCGACGCCGGCTCGGAAGGCACGCTCGCCCGCATGGATCTGAGCGGTCTGGCCG
AAGAACTCGCCGAGCTGTTCGATCCGGCCTGCGAGGAAGCCAGCCTCACCTTCAAAAGCCAGATCGCCCGCAACCTGGTC
ATCCTCGGCGACAAGGATTTGATCGGCCAGGCGCTGGCAAACCTCTTGGACAACGCCGTGAAATACACCCCCGCAGGCGG
CGCCATCAGCTTCACCGCCAGCCGCACGCCCGAAGGTATGATCGACCTGACGGTCATCGATTCCGGCCCCGGCATTCCCG
AAGAGGCGCGCGACAAGGTCATCGAACGTTTTCACCGGCTCGACTCTGCGCGCACACAGCCCGGCTCGGGCCTTGGCCTC
GCCCTCGTGGAATCTGTCGCTGAGTTGCATCGCGGCCAGCTGATCCTCGGCACAGGCAACGGCCCGACCGAACAGCCCGG
CCTGAAGGCTACGCTTCGTCTGCCGCGGGCTTGA

Upstream 100 bases:

>100_bases
GGAGTTCGCCGAACCGCTGCTCCAGACCGTTCGCGGCGCCGGATACCGCCTGCAGAGCCAGGGCTAGACCTTTTGGAGCG
GCGCTGGCACCTAGCGCGAG

Downstream 100 bases:

>100_bases
GCCGCCGCCCTGCCGGCCACATCCCGATCAGCCCGCCAAGCCCGATCAGCACGACGCCCAGATACAGCACATCCACCATC
GGATTGTAGTAGACGCGGAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKTPLQLPAWLAPRVPDRTQLLKPLRILGKGIGAGWRGLGKAWSSLPFMAQIKKAMPAFARTTTFKLTILYSAMIGAFSG
ALLVYLYYSTVYYIRAESENRITVEFEQLANAYYTGGMERLSQSVFERMTLSGSQFFYYLEDSSGRKIAGHFPRLPADPP
AAGMKTVYFDFELPQADGTNTLRPAAGRIVRLRDNGGALMVAFDTAQQTVIVGRIRNAVYVAMPIALILSLAGGLVISRG
AAKRADELAKTTEAVMGGELGRRMPVRGTGDEFDRLAQRLNAMLDQIQKLVESSRHTGNAIAHDLRSPLTRLRNRLELAL
SKPLSEEAANETLGTTLSEVDRVLDTFNAILRLARLDAGSEGTLARMDLSGLAEELAELFDPACEEASLTFKSQIARNLV
ILGDKDLIGQALANLLDNAVKYTPAGGAISFTASRTPEGMIDLTVIDSGPGIPEEARDKVIERFHRLDSARTQPGSGLGL
ALVESVAELHRGQLILGTGNGPTEQPGLKATLRLPRA

Sequences:

>Translated_517_residues
MKTPLQLPAWLAPRVPDRTQLLKPLRILGKGIGAGWRGLGKAWSSLPFMAQIKKAMPAFARTTTFKLTILYSAMIGAFSG
ALLVYLYYSTVYYIRAESENRITVEFEQLANAYYTGGMERLSQSVFERMTLSGSQFFYYLEDSSGRKIAGHFPRLPADPP
AAGMKTVYFDFELPQADGTNTLRPAAGRIVRLRDNGGALMVAFDTAQQTVIVGRIRNAVYVAMPIALILSLAGGLVISRG
AAKRADELAKTTEAVMGGELGRRMPVRGTGDEFDRLAQRLNAMLDQIQKLVESSRHTGNAIAHDLRSPLTRLRNRLELAL
SKPLSEEAANETLGTTLSEVDRVLDTFNAILRLARLDAGSEGTLARMDLSGLAEELAELFDPACEEASLTFKSQIARNLV
ILGDKDLIGQALANLLDNAVKYTPAGGAISFTASRTPEGMIDLTVIDSGPGIPEEARDKVIERFHRLDSARTQPGSGLGL
ALVESVAELHRGQLILGTGNGPTEQPGLKATLRLPRA
>Mature_517_residues
MKTPLQLPAWLAPRVPDRTQLLKPLRILGKGIGAGWRGLGKAWSSLPFMAQIKKAMPAFARTTTFKLTILYSAMIGAFSG
ALLVYLYYSTVYYIRAESENRITVEFEQLANAYYTGGMERLSQSVFERMTLSGSQFFYYLEDSSGRKIAGHFPRLPADPP
AAGMKTVYFDFELPQADGTNTLRPAAGRIVRLRDNGGALMVAFDTAQQTVIVGRIRNAVYVAMPIALILSLAGGLVISRG
AAKRADELAKTTEAVMGGELGRRMPVRGTGDEFDRLAQRLNAMLDQIQKLVESSRHTGNAIAHDLRSPLTRLRNRLELAL
SKPLSEEAANETLGTTLSEVDRVLDTFNAILRLARLDAGSEGTLARMDLSGLAEELAELFDPACEEASLTFKSQIARNLV
ILGDKDLIGQALANLLDNAVKYTPAGGAISFTASRTPEGMIDLTVIDSGPGIPEEARDKVIERFHRLDSARTQPGSGLGL
ALVESVAELHRGQLILGTGNGPTEQPGLKATLRLPRA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790861, Length=306, Percent_Identity=30.718954248366, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786783, Length=284, Percent_Identity=27.4647887323944, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788279, Length=279, Percent_Identity=28.3154121863799, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786600, Length=211, Percent_Identity=30.8056872037915, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=28.8461538461538, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787894, Length=344, Percent_Identity=30.8139534883721, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789808, Length=255, Percent_Identity=30.1960784313725, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790346, Length=262, Percent_Identity=27.8625954198473, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789403, Length=296, Percent_Identity=28.7162162162162, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790551, Length=320, Percent_Identity=27.5, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787374, Length=239, Percent_Identity=24.6861924686192, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788549, Length=215, Percent_Identity=27.4418604651163, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=25.3393665158371, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786912, Length=203, Percent_Identity=30.0492610837438, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=28.3842794759825, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56102; Mature: 56102

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPLQLPAWLAPRVPDRTQLLKPLRILGKGIGAGWRGLGKAWSSLPFMAQIKKAMPAFA
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RTTTFKLTILYSAMIGAFSGALLVYLYYSTVYYIRAESENRITVEFEQLANAYYTGGMER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHCCCHHH
LSQSVFERMTLSGSQFFYYLEDSSGRKIAGHFPRLPADPPAAGMKTVYFDFELPQADGTN
HHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
TLRPAAGRIVRLRDNGGALMVAFDTAQQTVIVGRIRNAVYVAMPIALILSLAGGLVISRG
CCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
AAKRADELAKTTEAVMGGELGRRMPVRGTGDEFDRLAQRLNAMLDQIQKLVESSRHTGNA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IAHDLRSPLTRLRNRLELALSKPLSEEAANETLGTTLSEVDRVLDTFNAILRLARLDAGS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGTLARMDLSGLAEELAELFDPACEEASLTFKSQIARNLVILGDKDLIGQALANLLDNAV
CCCEEECCHHHHHHHHHHHHCCCCCHHCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHH
KYTPAGGAISFTASRTPEGMIDLTVIDSGPGIPEEARDKVIERFHRLDSARTQPGSGLGL
EECCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
ALVESVAELHRGQLILGTGNGPTEQPGLKATLRLPRA
HHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MKTPLQLPAWLAPRVPDRTQLLKPLRILGKGIGAGWRGLGKAWSSLPFMAQIKKAMPAFA
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RTTTFKLTILYSAMIGAFSGALLVYLYYSTVYYIRAESENRITVEFEQLANAYYTGGMER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEHHHHHHHHHCCCHHH
LSQSVFERMTLSGSQFFYYLEDSSGRKIAGHFPRLPADPPAAGMKTVYFDFELPQADGTN
HHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
TLRPAAGRIVRLRDNGGALMVAFDTAQQTVIVGRIRNAVYVAMPIALILSLAGGLVISRG
CCCCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
AAKRADELAKTTEAVMGGELGRRMPVRGTGDEFDRLAQRLNAMLDQIQKLVESSRHTGNA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IAHDLRSPLTRLRNRLELALSKPLSEEAANETLGTTLSEVDRVLDTFNAILRLARLDAGS
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGTLARMDLSGLAEELAELFDPACEEASLTFKSQIARNLVILGDKDLIGQALANLLDNAV
CCCEEECCHHHHHHHHHHHHCCCCCHHCHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHH
KYTPAGGAISFTASRTPEGMIDLTVIDSGPGIPEEARDKVIERFHRLDSARTQPGSGLGL
EECCCCCEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHH
ALVESVAELHRGQLILGTGNGPTEQPGLKATLRLPRA
HHHHHHHHHHCCEEEEECCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]