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The map label for this gene is dosC [H]

Identifier: 114799892

GI number: 114799892

Start: 3640179

End: 3640865

Strand: Reverse

Name: dosC [H]

Synonym: HNE_3504

Alternate gene names: 114799892

Gene position: 3640865-3640179 (Counterclockwise)

Preceding gene: 114799300

Following gene: 114797747

Centisome position: 98.27

GC content: 66.08

Gene sequence:

>687_bases
ATGGCAACTGACACCCCGGAGCTCACCGAAGCTGAAAAGCGCGCCCTGCTGGACGCGCTGGGGGTTGACCTTGCCCGTCT
GGACCTGCGCGCCCGCGCGGCCATCGAGGCGCTCGCCGAAGAAGTCATCGTTCTGCGCGAAGAGGCCGACACGCTACGGG
CCAGCTTGGCGGACGCCGCGCTGCTGGCCGATAATGACGCGCTCTGCCCGGTCTTCAACCGCCGGGCTTTCGAGCGGGAA
GTGCGCCGCGAAATTGCGCTGTCGGGCCGCTTCCAGACGCCTTTGACGCTGATTTTCATCGATCTGGACAATTTCAAGCA
GGTGAACGACGTCTACGGCCACGCCGCCGGGGACAGCGTTCTCCTGAAGATTTCCGACATTCTCCTGGCCCGCACCCGCG
AAACCGACATTGTCGGCCGCCTCGGCGGGGACGAATTCGGCATCGTGCTCGCCCATGCGAGCCTCGAAGACAGCCGGCGC
AAAGCGGCCCAGCTCGCCGCCGCCATTGATGCGCTGGAAGTGCGCGGCGAAGAGGGGGGCGGTCTCGCCCCCGTCCAGAT
CGGCGCATCCTGCGGCATTGTCGAATGGCATGCCGGCCAGAGCCCGGCCGCGCTCATCGCGCGCGCAGATCAGGCAATGT
TTGCCGAAAAATCGCGCCGGAAGGCCGAAGGACGCGCATTAAGGTGA

Upstream 100 bases:

>100_bases
GAGCGCGCTGCTGACCGTCAACCGCTCCTGTGAGGGGAAAGTTGGCGCGCCCCGTGAACGGGCTCTCTACAAGCGAAGCG
AACAAAGTTAGTGTTAACAC

Downstream 100 bases:

>100_bases
AAATGCGGCAAGATAGGTAGTTCTGCCCATGACACCCCTACACGAGTGTGGCATTCTTGCATCACCGGTCAATCCGGGCC
GGGGACGAAAGGAGACTGCC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MATDTPELTEAEKRALLDALGVDLARLDLRARAAIEALAEEVIVLREEADTLRASLADAALLADNDALCPVFNRRAFERE
VRREIALSGRFQTPLTLIFIDLDNFKQVNDVYGHAAGDSVLLKISDILLARTRETDIVGRLGGDEFGIVLAHASLEDSRR
KAAQLAAAIDALEVRGEEGGGLAPVQIGASCGIVEWHAGQSPAALIARADQAMFAEKSRRKAEGRALR

Sequences:

>Translated_228_residues
MATDTPELTEAEKRALLDALGVDLARLDLRARAAIEALAEEVIVLREEADTLRASLADAALLADNDALCPVFNRRAFERE
VRREIALSGRFQTPLTLIFIDLDNFKQVNDVYGHAAGDSVLLKISDILLARTRETDIVGRLGGDEFGIVLAHASLEDSRR
KAAQLAAAIDALEVRGEEGGGLAPVQIGASCGIVEWHAGQSPAALIARADQAMFAEKSRRKAEGRALR
>Mature_227_residues
ATDTPELTEAEKRALLDALGVDLARLDLRARAAIEALAEEVIVLREEADTLRASLADAALLADNDALCPVFNRRAFEREV
RREIALSGRFQTPLTLIFIDLDNFKQVNDVYGHAAGDSVLLKISDILLARTRETDIVGRLGGDEFGIVLAHASLEDSRRK
AAQLAAAIDALEVRGEEGGGLAPVQIGASCGIVEWHAGQSPAALIARADQAMFAEKSRRKAEGRALR

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=154, Percent_Identity=35.0649350649351, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=34.2696629213483, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=34.9112426035503, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=29.3413173652695, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=98, Percent_Identity=33.6734693877551, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082007, Length=150, Percent_Identity=30, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787816, Length=143, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=29.7619047619048, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1787541, Length=158, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788085, Length=133, Percent_Identity=30.8270676691729, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 24578; Mature: 24447

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDTPELTEAEKRALLDALGVDLARLDLRARAAIEALAEEVIVLREEADTLRASLADAA
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLADNDALCPVFNRRAFEREVRREIALSGRFQTPLTLIFIDLDNFKQVNDVYGHAAGDSV
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCE
LLKISDILLARTRETDIVGRLGGDEFGIVLAHASLEDSRRKAAQLAAAIDALEVRGEEGG
EEEHHHHHHHHCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
GLAPVQIGASCGIVEWHAGQSPAALIARADQAMFAEKSRRKAEGRALR
CCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATDTPELTEAEKRALLDALGVDLARLDLRARAAIEALAEEVIVLREEADTLRASLADAA
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLADNDALCPVFNRRAFEREVRREIALSGRFQTPLTLIFIDLDNFKQVNDVYGHAAGDSV
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCCE
LLKISDILLARTRETDIVGRLGGDEFGIVLAHASLEDSRRKAAQLAAAIDALEVRGEEGG
EEEHHHHHHHHCCCCCEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
GLAPVQIGASCGIVEWHAGQSPAALIARADQAMFAEKSRRKAEGRALR
CCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]