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The map label for this gene is tyrS [H]

Identifier: 114799842

GI number: 114799842

Start: 2783188

End: 2784438

Strand: Direct

Name: tyrS [H]

Synonym: HNE_2625

Alternate gene names: 114799842

Gene position: 2783188-2784438 (Clockwise)

Preceding gene: 114798282

Following gene: 114797554

Centisome position: 75.12

GC content: 64.35

Gene sequence:

>1251_bases
ATGAGCGATTACAAGTCGGAATTCCTGCGCGAGCTGAGCTGGCGCGGCTTCATCAAGGACACGACTCACAAGGCTGAGCT
CGACGCCTATTGCAGCAACGGAACGCCGGTCGCTTATGTGGGCTATGACGCCACCGCCGACAGCCTGCATGTGGGCCATC
TGATGACGATCATGATGCTGCGCATTTTCCAGCGCCATGGCGGCAAGCCGATTGCCCTGATGGGCGGAGGCACGACCAAG
GTGGGCGACCCGACCGACAAGGAAAAGTCCCGCCCCCTGCTGACCGATGACCAGATCAACGCCAACATTGCGGGCATCAA
GACCGCGTTCGAGCCGTTCCTGCGCTTTGGCGACGGGGCCAATGACGCCATCATGGTCAACAACAATGACTGGCTGGGCG
AGCTGGGCTATCTCGAAATGCTGCGCGCGATCGGCGTCTATTTCACCATCAATACGATGGTGAAGCAGGACACCGTGCGC
CGCCGCCTGGAGGCCGAGCAGCCCTACACCTTCCTCGAATTCAACTACCTGCTGATGCAGTCCTACGACTTCCTCGAGCT
GTTCCGCCGCCATGGCTGCCGGATGCAGCTGGGCGGGTCTGACCAGTGGGGCAATATCGTGGGCGGGGTGGACCTTGTGC
ACAAGGCCGAGGGCGGGGACGCCTTTGGCGTGACGGCGCACCTTGTGACCACCTCTTCGGGCGTGAAGATGGGCAAGACG
GTGGGCGGGGCCGTGTGGCTGAACGCCGACCGCAAGAGCCCGTATGAATACTGGCAGTTCTGGCGCAACACCGAAGACGC
CGACGTGGGCAAGTTCCTGCGGCTGTTCACCGACCTTGGCCGGGAGGAAATCGAGCGGCTTGAGGCGCTGGAAGGCTCAG
CGATCAACGACGCGAAGATTGCGCTGGCGAACGCGGCGACGACGCTGTTGCATGGGGCGGCCGCTGCGGCGGAGGCTGAG
GCGGCGGCGAAGGCGGTGTTTGCGGGCGGGGCTTCGGCCGATGCGTTGCCGACGGCGCTTGTTCCGCAGGCGGAGCTTGA
GGCCGGAATGCTGGTGGCGGCGGCGTGCGCCGCGGCGGGGCTGTCCAAGTCCAATGGCGAGGCGCGGCGGCTGATCGAGC
AGGGCGCGATCCGGGTGAATGACGCGCTGGTGTCTGATCCCAATGCCAAAGTCTCGATGGCCGACATGCAGGACGGGGCG
ATCAAGCTGTCGGCTGGCAAGAAGCGGCATGCGCTGGTGAAGGCGGGGTAA

Upstream 100 bases:

>100_bases
TGAAGCCGGCGACCCATTGGGGGGCGGTTTTTTCAATGGCGGTGTTCAAGGCAGGCCCTTTCCATGCTAGGCGCGCGCAT
CTCATCGGAGACACGAGAAC

Downstream 100 bases:

>100_bases
GACCCGCCTAGAACTCTTCTTCCTTCAGCGGGGGCAGCTCTTGCGGCAGGGCCTGGTCCGGCGCGCGCGGCGTTGCCTCC
GGGATTTTGGTGTCGGACGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSDYKSEFLRELSWRGFIKDTTHKAELDAYCSNGTPVAYVGYDATADSLHVGHLMTIMMLRIFQRHGGKPIALMGGGTTK
VGDPTDKEKSRPLLTDDQINANIAGIKTAFEPFLRFGDGANDAIMVNNNDWLGELGYLEMLRAIGVYFTINTMVKQDTVR
RRLEAEQPYTFLEFNYLLMQSYDFLELFRRHGCRMQLGGSDQWGNIVGGVDLVHKAEGGDAFGVTAHLVTTSSGVKMGKT
VGGAVWLNADRKSPYEYWQFWRNTEDADVGKFLRLFTDLGREEIERLEALEGSAINDAKIALANAATTLLHGAAAAAEAE
AAAKAVFAGGASADALPTALVPQAELEAGMLVAAACAAAGLSKSNGEARRLIEQGAIRVNDALVSDPNAKVSMADMQDGA
IKLSAGKKRHALVKAG

Sequences:

>Translated_416_residues
MSDYKSEFLRELSWRGFIKDTTHKAELDAYCSNGTPVAYVGYDATADSLHVGHLMTIMMLRIFQRHGGKPIALMGGGTTK
VGDPTDKEKSRPLLTDDQINANIAGIKTAFEPFLRFGDGANDAIMVNNNDWLGELGYLEMLRAIGVYFTINTMVKQDTVR
RRLEAEQPYTFLEFNYLLMQSYDFLELFRRHGCRMQLGGSDQWGNIVGGVDLVHKAEGGDAFGVTAHLVTTSSGVKMGKT
VGGAVWLNADRKSPYEYWQFWRNTEDADVGKFLRLFTDLGREEIERLEALEGSAINDAKIALANAATTLLHGAAAAAEAE
AAAKAVFAGGASADALPTALVPQAELEAGMLVAAACAAAGLSKSNGEARRLIEQGAIRVNDALVSDPNAKVSMADMQDGA
IKLSAGKKRHALVKAG
>Mature_415_residues
SDYKSEFLRELSWRGFIKDTTHKAELDAYCSNGTPVAYVGYDATADSLHVGHLMTIMMLRIFQRHGGKPIALMGGGTTKV
GDPTDKEKSRPLLTDDQINANIAGIKTAFEPFLRFGDGANDAIMVNNNDWLGELGYLEMLRAIGVYFTINTMVKQDTVRR
RLEAEQPYTFLEFNYLLMQSYDFLELFRRHGCRMQLGGSDQWGNIVGGVDLVHKAEGGDAFGVTAHLVTTSSGVKMGKTV
GGAVWLNADRKSPYEYWQFWRNTEDADVGKFLRLFTDLGREEIERLEALEGSAINDAKIALANAATTLLHGAAAAAEAEA
AAKAVFAGGASADALPTALVPQAELEAGMLVAAACAAAGLSKSNGEARRLIEQGAIRVNDALVSDPNAKVSMADMQDGAI
KLSAGKKRHALVKAG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=428, Percent_Identity=32.4766355140187, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI1787925, Length=418, Percent_Identity=38.9952153110048, Blast_Score=300, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI25145802, Length=430, Percent_Identity=30.4651162790698, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=303, Percent_Identity=30.3630363036304, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=29.5045045045045, Blast_Score=206, Evalue=7e-54,
Organism=Drosophila melanogaster, GI20130383, Length=437, Percent_Identity=32.4942791762014, Blast_Score=221, Evalue=6e-58,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44932; Mature: 44800

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYKSEFLRELSWRGFIKDTTHKAELDAYCSNGTPVAYVGYDATADSLHVGHLMTIMML
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
RIFQRHGGKPIALMGGGTTKVGDPTDKEKSRPLLTDDQINANIAGIKTAFEPFLRFGDGA
HHHHHCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NDAIMVNNNDWLGELGYLEMLRAIGVYFTINTMVKQDTVRRRLEAEQPYTFLEFNYLLMQ
CCEEEECCCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHC
SYDFLELFRRHGCRMQLGGSDQWGNIVGGVDLVHKAEGGDAFGVTAHLVTTSSGVKMGKT
CCHHHHHHHHCCCEEEECCCCCCCCHHCCHHHEEECCCCCEECEEEEEEEECCCCCCCCC
VGGAVWLNADRKSPYEYWQFWRNTEDADVGKFLRLFTDLGREEIERLEALEGSAINDAKI
CCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHE
ALANAATTLLHGAAAAAEAEAAAKAVFAGGASADALPTALVPQAELEAGMLVAAACAAAG
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHC
LSKSNGEARRLIEQGAIRVNDALVSDPNAKVSMADMQDGAIKLSAGKKRHALVKAG
CCCCCCHHHHHHHHCCEEECHHEECCCCCEEEEEECCCCCEEEECCCCCCEEEECC
>Mature Secondary Structure 
SDYKSEFLRELSWRGFIKDTTHKAELDAYCSNGTPVAYVGYDATADSLHVGHLMTIMML
CHHHHHHHHHHHCCCCCCCCCCHHHHHHEECCCCEEEEEECCCCCCCCHHHHHHHHHHH
RIFQRHGGKPIALMGGGTTKVGDPTDKEKSRPLLTDDQINANIAGIKTAFEPFLRFGDGA
HHHHHCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
NDAIMVNNNDWLGELGYLEMLRAIGVYFTINTMVKQDTVRRRLEAEQPYTFLEFNYLLMQ
CCEEEECCCCCCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHC
SYDFLELFRRHGCRMQLGGSDQWGNIVGGVDLVHKAEGGDAFGVTAHLVTTSSGVKMGKT
CCHHHHHHHHCCCEEEECCCCCCCCHHCCHHHEEECCCCCEECEEEEEEEECCCCCCCCC
VGGAVWLNADRKSPYEYWQFWRNTEDADVGKFLRLFTDLGREEIERLEALEGSAINDAKI
CCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHE
ALANAATTLLHGAAAAAEAEAAAKAVFAGGASADALPTALVPQAELEAGMLVAAACAAAG
EHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHC
LSKSNGEARRLIEQGAIRVNDALVSDPNAKVSMADMQDGAIKLSAGKKRHALVKAG
CCCCCCHHHHHHHHCCEEECHHEECCCCCEEEEEECCCCCEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA