Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 114799841

GI number: 114799841

Start: 3512431

End: 3513702

Strand: Direct

Name: rsmB [H]

Synonym: HNE_3368

Alternate gene names: 114799841

Gene position: 3512431-3513702 (Clockwise)

Preceding gene: 114798607

Following gene: 114798828

Centisome position: 94.8

GC content: 69.34

Gene sequence:

>1272_bases
TTGAGCGGCGCCCCTATCCGGTTGGCCGCGGGCCGCCTTCTGAACCTCGTGCTCGACAAGAAGCGCACGCTCGACGACGC
GCTGGCGACGGACGATGCATATAACAATCTCGACGGTTCTGACCGCGCCTTTGCGCGCGCCATTGCCTCGTCTGCCCTGC
GCCATCTGGGCTGGATCGACCGGGGCGTTTCGCCGTTCCTGTCGCGGCCGCTTTCGGCCACCGGCGCGGAGATCCGCGCA
CTGATCCGGGCGGGCGCGGCGCAGATCTGGTTCATGGATGTGCCCCATCATGCCGCCGTTTCCGAAACGGTGGAGGCCGC
GCGGCTCTGGTCGCCGGCGCGCTCAGGCGGCGGGTTTCTGAATGCCGCGCTGCGCCGGGTGACCGAAAGCGAAAGTCCGG
CTGCCACGATCAGCCCGCTCGCGCTCTGGCCCGACTGGCTGGCGAGCCGGCTGGGCTCTGCCTATGGCCGCGAGGCGGCT
GAAGCCCTCGCCCTCTCTCAACTGGAAGAACCAAGCCTGCACCTCTCCCCCAAGGGCGACGGCGCCCGCGTGGCCGAGGC
GACCGGGGGCACGCTGCTGCCCGATGGCGCCATCGACATCGTAAGTGGCGCGGTGGAGACCCTGCCGGGCTACGCCGCAG
GCGACTGGTGGGTGCAGGACGCGGCCGCGCGCATTCCCGCTCAGCTTCTTTCCCCCCAAACGGGGGAGCGCATTCTCGAC
CTCTGCGCCGCGCCGGGGGGCAAGACGTTGCAGCTCGCCGCCGCGGGCGCTGACGTCACCGCGCTCGACCGGTCGCGCCC
GCGCCTCGAGCGGCTGAAGGAAAACCTCGCCCGCACCGGCCTTCACGCCGAAGTGATCATCGCCGATGGCGAGGCGTGGG
CCCCGCCCGCCCTCTTCCCGAAAATCCTGCTGGACGCGCCCTGCTCGGCCCTTGGCACCCTGCGGCGCCACCCCGAAAGC
GCCTGGATCAAGACCGAAGACGATGTTGCGCGCTTTCCGGGCGTGCAGGCGCGCCTTCTGGCGGCCGCCGCAGGCATGCT
GGCGCCCGGCGGAACGCTGGTTTACTGCGTGTGTACGCCGGTTCCAGCCGAAGGGGTGGACATTGTGGAGGCCGCGATCG
GCAAGGGAATTCTGACCCGGTCGCCGGTAAAGGCCGAAGAAATTCCAGGCTTTGAAGCCGCGCTGACGCCGGCAGGCGAT
GTGCTGACATTGCCCGGAACGGGCGGAAAACACGACGCGTTCTATATTGCCCGGCTGATCAAACCGGCCTGA

Upstream 100 bases:

>100_bases
ATGGCGGGCGAGATGGGCATCTCGGGCGTGGCAGCCCCAACCGCGCGCTCCAGCGGGCCTTGGGGTTGACCCTTACAATT
CTAATCCGGAGGTCCTCGGT

Downstream 100 bases:

>100_bases
CCTGTCCAAACGGTAATCGTTTTCTGAACGGAAAACCGCACGGCAGTTCATATGTCATTCAGCAGATCCTGCGGTATAAG
ACGCCCATCGCAGATATGCA

Product: putative ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSGAPIRLAAGRLLNLVLDKKRTLDDALATDDAYNNLDGSDRAFARAIASSALRHLGWIDRGVSPFLSRPLSATGAEIRA
LIRAGAAQIWFMDVPHHAAVSETVEAARLWSPARSGGGFLNAALRRVTESESPAATISPLALWPDWLASRLGSAYGREAA
EALALSQLEEPSLHLSPKGDGARVAEATGGTLLPDGAIDIVSGAVETLPGYAAGDWWVQDAAARIPAQLLSPQTGERILD
LCAAPGGKTLQLAAAGADVTALDRSRPRLERLKENLARTGLHAEVIIADGEAWAPPALFPKILLDAPCSALGTLRRHPES
AWIKTEDDVARFPGVQARLLAAAAGMLAPGGTLVYCVCTPVPAEGVDIVEAAIGKGILTRSPVKAEEIPGFEAALTPAGD
VLTLPGTGGKHDAFYIARLIKPA

Sequences:

>Translated_423_residues
MSGAPIRLAAGRLLNLVLDKKRTLDDALATDDAYNNLDGSDRAFARAIASSALRHLGWIDRGVSPFLSRPLSATGAEIRA
LIRAGAAQIWFMDVPHHAAVSETVEAARLWSPARSGGGFLNAALRRVTESESPAATISPLALWPDWLASRLGSAYGREAA
EALALSQLEEPSLHLSPKGDGARVAEATGGTLLPDGAIDIVSGAVETLPGYAAGDWWVQDAAARIPAQLLSPQTGERILD
LCAAPGGKTLQLAAAGADVTALDRSRPRLERLKENLARTGLHAEVIIADGEAWAPPALFPKILLDAPCSALGTLRRHPES
AWIKTEDDVARFPGVQARLLAAAAGMLAPGGTLVYCVCTPVPAEGVDIVEAAIGKGILTRSPVKAEEIPGFEAALTPAGD
VLTLPGTGGKHDAFYIARLIKPA
>Mature_422_residues
SGAPIRLAAGRLLNLVLDKKRTLDDALATDDAYNNLDGSDRAFARAIASSALRHLGWIDRGVSPFLSRPLSATGAEIRAL
IRAGAAQIWFMDVPHHAAVSETVEAARLWSPARSGGGFLNAALRRVTESESPAATISPLALWPDWLASRLGSAYGREAAE
ALALSQLEEPSLHLSPKGDGARVAEATGGTLLPDGAIDIVSGAVETLPGYAAGDWWVQDAAARIPAQLLSPQTGERILDL
CAAPGGKTLQLAAAGADVTALDRSRPRLERLKENLARTGLHAEVIIADGEAWAPPALFPKILLDAPCSALGTLRRHPESA
WIKTEDDVARFPGVQARLLAAAAGMLAPGGTLVYCVCTPVPAEGVDIVEAAIGKGILTRSPVKAEEIPGFEAALTPAGDV
LTLPGTGGKHDAFYIARLIKPA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=187, Percent_Identity=36.3636363636364, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI76150625, Length=177, Percent_Identity=36.1581920903955, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI76150623, Length=177, Percent_Identity=36.1581920903955, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI40316918, Length=172, Percent_Identity=32.5581395348837, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI11545785, Length=233, Percent_Identity=29.1845493562232, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI2367212, Length=440, Percent_Identity=31.3636363636364, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87081985, Length=225, Percent_Identity=29.7777777777778, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=207, Percent_Identity=31.8840579710145, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=213, Percent_Identity=30.9859154929577, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI22024126, Length=265, Percent_Identity=29.811320754717, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356513, Length=247, Percent_Identity=34.412955465587, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24668781, Length=247, Percent_Identity=34.412955465587, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 44144; Mature: 44013

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAPIRLAAGRLLNLVLDKKRTLDDALATDDAYNNLDGSDRAFARAIASSALRHLGWID
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGVSPFLSRPLSATGAEIRALIRAGAAQIWFMDVPHHAAVSETVEAARLWSPARSGGGFL
CCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHH
NAALRRVTESESPAATISPLALWPDWLASRLGSAYGREAAEALALSQLEEPSLHLSPKGD
HHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
GARVAEATGGTLLPDGAIDIVSGAVETLPGYAAGDWWVQDAAARIPAQLLSPQTGERILD
CCEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHH
LCAAPGGKTLQLAAAGADVTALDRSRPRLERLKENLARTGLHAEVIIADGEAWAPPALFP
HHCCCCCCEEEEEECCCCEEHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
KILLDAPCSALGTLRRHPESAWIKTEDDVARFPGVQARLLAAAAGMLAPGGTLVYCVCTP
HHHHHCCHHHHHHHHHCCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
VPAEGVDIVEAAIGKGILTRSPVKAEEIPGFEAALTPAGDVLTLPGTGGKHDAFYIARLI
CCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCEECCCCCCEEECCCCCCCCHHHHHHHHH
KPA
CCC
>Mature Secondary Structure 
SGAPIRLAAGRLLNLVLDKKRTLDDALATDDAYNNLDGSDRAFARAIASSALRHLGWID
CCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RGVSPFLSRPLSATGAEIRALIRAGAAQIWFMDVPHHAAVSETVEAARLWSPARSGGGFL
CCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHH
NAALRRVTESESPAATISPLALWPDWLASRLGSAYGREAAEALALSQLEEPSLHLSPKGD
HHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
GARVAEATGGTLLPDGAIDIVSGAVETLPGYAAGDWWVQDAAARIPAQLLSPQTGERILD
CCEEEECCCCEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHCCCCHHHHHH
LCAAPGGKTLQLAAAGADVTALDRSRPRLERLKENLARTGLHAEVIIADGEAWAPPALFP
HHCCCCCCEEEEEECCCCEEHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
KILLDAPCSALGTLRRHPESAWIKTEDDVARFPGVQARLLAAAAGMLAPGGTLVYCVCTP
HHHHHCCHHHHHHHHHCCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
VPAEGVDIVEAAIGKGILTRSPVKAEEIPGFEAALTPAGDVLTLPGTGGKHDAFYIARLI
CCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCEECCCCCCEEECCCCCCCCHHHHHHHHH
KPA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA