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The map label for this gene is sppA [H]

Identifier: 114799830

GI number: 114799830

Start: 2797242

End: 2799020

Strand: Direct

Name: sppA [H]

Synonym: HNE_2638

Alternate gene names: 114799830

Gene position: 2797242-2799020 (Clockwise)

Preceding gene: 114799073

Following gene: 114798701

Centisome position: 75.5

GC content: 62.96

Gene sequence:

>1779_bases
ATGAAAACTTTTCTGCTCTCCATGGCAGGCGCCTTCACGGCGATGATCCTTTTCATCATCGTGGGCTTCTTTTTCCTCTT
CACCCTTATCGGCGTCGCGGCGAGCTCCAAGCCGCCCCATCCCGATGACATTATTCTTACGCTTGATCTCAACGCCGCCT
ATCCCGACCAGGCGCCCGCCGGCGGGCTTGCCGCCCTGTCGGGCACGCCGGGCTTCATTGATCTGCTGCTCAAGCTGAAA
GAAGCCGAATCGGACGATTCGGTGAAGGGCCTCTTCATCCGCGGCGCCGATTACGGCTTTGGCACCACCCGCGCCGAAGA
ACTGCGCGAAGCCATTCAGAGCTTCAAGGCGTCAGGCAAATTCGTCATCGCCCATTCGCAGGGCATGTTCGGCTCCAGCG
GCCCGTCGGCCTTTCACTCGATCTCGGCGGCCGATGAAATCTGGATGCAGCCGGGCACCGACCTGATGGTCACCGGCGTG
GTTTTCGAGACCGAATTCTACAAGGGCCTGTTCGAGAACATCGACCTGCAGCCGCAGGTCTATCCCTTCTATGAATACAA
GAACGCGCCCAACTCTTATAATGAGACGAGCTATACCGAGCCCCACCGCATGGCGATGGAAGCCCTGGCCACCTCGATCT
GGACCACGGCGCTGGAAGAGATTGCCGAAGATCGCGGCACCTCTGCCGGGCAGCTGCGCGCCGCGCTGGAAAGCGGCCCC
AAGCCGGCCGAAACCGCGCTTGAGCTGAAGCTGGTCGACAAGCTCGGCTGGCCCGAAGACGCTGAAGAAGCCGCCATTGC
CCGCGCCGGCAATGACGACGCTGAAATGATTGACCTCGCGGTCTATGCCGCGCCCACCAAATATAACAGCAAGGCTCCGC
TGATCGCTGTTGTGGGCGGCGAAGGCCCCATCGTGACCGGCGGCGCCGATGGCGGCTCGCCTTTCTCTGATGCGCCCGCC
TTTGCATCCGACACCGTCGCCCGCGCCATTCTGGACGCAGCCGAAGACGAAGACGTCAAAGCCATCGTCTTCCGTGTCGA
CAGCCCCGGAGGCTCACCGACTGCGTCTGACCAGATCTGGCGCGCGGTGGAACGCGCCAAGGAAGCGGGCAAACCGGTTG
TTGTCTCGATGGGCGCCTATGCGGCTTCGGGCGGGTATTATGTCTCGACCGGCGCAGACGCGATCCTGGCCAACCGCGCG
ACGCTGACCGGCTCGATCGGCATCTTCGGCGGCAAGCTCGCCCTGGATGGCACCTTCAACAAGATCGGCGTCACCTTTGA
CTCGGTAACGGTCGGCGGCGATTTCGCCTCCGCCTGGGGCACCTCGCCCTTTACCGAAACCCAGGAAGCCGAAGTGAAGG
CCAGCCTGAAACGCGGCTATGACCGCTTCCTCAACCATGTCGGCGCCGGCCGCGACATGACCTATGATGAAGTGCATGAA
GTGGCCCGCGGTCGCGTGTGGACGGGTGAAGCCGCCCTGCAGCAAGGCCTGGTCGACGAGATCGGCACCTTCATGGACGC
CATCGAGAAAGCCAAGGAACTGGCCGGCATCGAAGCCGATGTGAAACCGCGCCTTGCCTTCTATCCCTATCGCAAGACGG
GCCTCGAAGCGCTGGAAGACCTGTTTGGCGTTTCCGCAGAAACCGCGCGCGCCGCCGCCGTGATCAGCACGATAGCCGGG
GATGAGCGCACGCAGGCGATGCTTCAGGAGCTGGCCACGGCCGAGGCCATGAATGCCGGACAGGCCATGGCCCTTGGCCC
GCGCATCCGCGAACGCTGA

Upstream 100 bases:

>100_bases
CCACAGCACAGCAGCTCTTTACATTTTATCGATTATCAACAATATCCATTTTGAACATATTCGCGCATCCTGCGCGCCCC
TTATGGAAGGCCTCCCGGAA

Downstream 100 bases:

>100_bases
TCGCGCCCGCAATTTATCTATCGCAGCACCCTCGCCGAACTGGCGGGGGTGTTGCAGTTTAAGGGGAATTGATACCCTGC
CCTTCACTCCGGCCTCCTCC

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MKTFLLSMAGAFTAMILFIIVGFFFLFTLIGVAASSKPPHPDDIILTLDLNAAYPDQAPAGGLAALSGTPGFIDLLLKLK
EAESDDSVKGLFIRGADYGFGTTRAEELREAIQSFKASGKFVIAHSQGMFGSSGPSAFHSISAADEIWMQPGTDLMVTGV
VFETEFYKGLFENIDLQPQVYPFYEYKNAPNSYNETSYTEPHRMAMEALATSIWTTALEEIAEDRGTSAGQLRAALESGP
KPAETALELKLVDKLGWPEDAEEAAIARAGNDDAEMIDLAVYAAPTKYNSKAPLIAVVGGEGPIVTGGADGGSPFSDAPA
FASDTVARAILDAAEDEDVKAIVFRVDSPGGSPTASDQIWRAVERAKEAGKPVVVSMGAYAASGGYYVSTGADAILANRA
TLTGSIGIFGGKLALDGTFNKIGVTFDSVTVGGDFASAWGTSPFTETQEAEVKASLKRGYDRFLNHVGAGRDMTYDEVHE
VARGRVWTGEAALQQGLVDEIGTFMDAIEKAKELAGIEADVKPRLAFYPYRKTGLEALEDLFGVSAETARAAAVISTIAG
DERTQAMLQELATAEAMNAGQAMALGPRIRER

Sequences:

>Translated_592_residues
MKTFLLSMAGAFTAMILFIIVGFFFLFTLIGVAASSKPPHPDDIILTLDLNAAYPDQAPAGGLAALSGTPGFIDLLLKLK
EAESDDSVKGLFIRGADYGFGTTRAEELREAIQSFKASGKFVIAHSQGMFGSSGPSAFHSISAADEIWMQPGTDLMVTGV
VFETEFYKGLFENIDLQPQVYPFYEYKNAPNSYNETSYTEPHRMAMEALATSIWTTALEEIAEDRGTSAGQLRAALESGP
KPAETALELKLVDKLGWPEDAEEAAIARAGNDDAEMIDLAVYAAPTKYNSKAPLIAVVGGEGPIVTGGADGGSPFSDAPA
FASDTVARAILDAAEDEDVKAIVFRVDSPGGSPTASDQIWRAVERAKEAGKPVVVSMGAYAASGGYYVSTGADAILANRA
TLTGSIGIFGGKLALDGTFNKIGVTFDSVTVGGDFASAWGTSPFTETQEAEVKASLKRGYDRFLNHVGAGRDMTYDEVHE
VARGRVWTGEAALQQGLVDEIGTFMDAIEKAKELAGIEADVKPRLAFYPYRKTGLEALEDLFGVSAETARAAAVISTIAG
DERTQAMLQELATAEAMNAGQAMALGPRIRER
>Mature_592_residues
MKTFLLSMAGAFTAMILFIIVGFFFLFTLIGVAASSKPPHPDDIILTLDLNAAYPDQAPAGGLAALSGTPGFIDLLLKLK
EAESDDSVKGLFIRGADYGFGTTRAEELREAIQSFKASGKFVIAHSQGMFGSSGPSAFHSISAADEIWMQPGTDLMVTGV
VFETEFYKGLFENIDLQPQVYPFYEYKNAPNSYNETSYTEPHRMAMEALATSIWTTALEEIAEDRGTSAGQLRAALESGP
KPAETALELKLVDKLGWPEDAEEAAIARAGNDDAEMIDLAVYAAPTKYNSKAPLIAVVGGEGPIVTGGADGGSPFSDAPA
FASDTVARAILDAAEDEDVKAIVFRVDSPGGSPTASDQIWRAVERAKEAGKPVVVSMGAYAASGGYYVSTGADAILANRA
TLTGSIGIFGGKLALDGTFNKIGVTFDSVTVGGDFASAWGTSPFTETQEAEVKASLKRGYDRFLNHVGAGRDMTYDEVHE
VARGRVWTGEAALQQGLVDEIGTFMDAIEKAKELAGIEADVKPRLAFYPYRKTGLEALEDLFGVSAETARAAAVISTIAG
DERTQAMLQELATAEAMNAGQAMALGPRIRER

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=464, Percent_Identity=32.7586206896552, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 62745; Mature: 62745

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFLLSMAGAFTAMILFIIVGFFFLFTLIGVAASSKPPHPDDIILTLDLNAAYPDQAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GGLAALSGTPGFIDLLLKLKEAESDDSVKGLFIRGADYGFGTTRAEELREAIQSFKASGK
CCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FVIAHSQGMFGSSGPSAFHSISAADEIWMQPGTDLMVTGVVFETEFYKGLFENIDLQPQV
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCE
YPFYEYKNAPNSYNETSYTEPHRMAMEALATSIWTTALEEIAEDRGTSAGQLRAALESGP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KPAETALELKLVDKLGWPEDAEEAAIARAGNDDAEMIDLAVYAAPTKYNSKAPLIAVVGG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHEEEEEEEECCCCCCCCCCEEEEECC
EGPIVTGGADGGSPFSDAPAFASDTVARAILDAAEDEDVKAIVFRVDSPGGSPTASDQIW
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHH
RAVERAKEAGKPVVVSMGAYAASGGYYVSTGADAILANRATLTGSIGIFGGKLALDGTFN
HHHHHHHHCCCCEEEEECCEECCCCEEEECCCCCCEECCCEEEECEEEECCEEEECCCCC
KIGVTFDSVTVGGDFASAWGTSPFTETQEAEVKASLKRGYDRFLNHVGAGRDMTYDEVHE
EEEEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VARGRVWTGEAALQQGLVDEIGTFMDAIEKAKELAGIEADVKPRLAFYPYRKTGLEALED
HHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
LFGVSAETARAAAVISTIAGDERTQAMLQELATAEAMNAGQAMALGPRIRER
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHCCC
>Mature Secondary Structure
MKTFLLSMAGAFTAMILFIIVGFFFLFTLIGVAASSKPPHPDDIILTLDLNAAYPDQAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCC
GGLAALSGTPGFIDLLLKLKEAESDDSVKGLFIRGADYGFGTTRAEELREAIQSFKASGK
CCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
FVIAHSQGMFGSSGPSAFHSISAADEIWMQPGTDLMVTGVVFETEFYKGLFENIDLQPQV
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCE
YPFYEYKNAPNSYNETSYTEPHRMAMEALATSIWTTALEEIAEDRGTSAGQLRAALESGP
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KPAETALELKLVDKLGWPEDAEEAAIARAGNDDAEMIDLAVYAAPTKYNSKAPLIAVVGG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHEEEEEEEECCCCCCCCCCEEEEECC
EGPIVTGGADGGSPFSDAPAFASDTVARAILDAAEDEDVKAIVFRVDSPGGSPTASDQIW
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHH
RAVERAKEAGKPVVVSMGAYAASGGYYVSTGADAILANRATLTGSIGIFGGKLALDGTFN
HHHHHHHHCCCCEEEEECCEECCCCEEEECCCCCCEECCCEEEECEEEECCEEEECCCCC
KIGVTFDSVTVGGDFASAWGTSPFTETQEAEVKASLKRGYDRFLNHVGAGRDMTYDEVHE
EEEEEEEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VARGRVWTGEAALQQGLVDEIGTFMDAIEKAKELAGIEADVKPRLAFYPYRKTGLEALED
HHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHH
LFGVSAETARAAAVISTIAGDERTQAMLQELATAEAMNAGQAMALGPRIRER
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]