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The map label for this gene is tpiA

Identifier: 114799671

GI number: 114799671

Start: 1837029

End: 1837778

Strand: Direct

Name: tpiA

Synonym: HNE_1792

Alternate gene names: 114799671

Gene position: 1837029-1837778 (Clockwise)

Preceding gene: 114799405

Following gene: 114799731

Centisome position: 49.58

GC content: 59.6

Gene sequence:

>750_bases
ATGCCACGCACGCTGATCGCCGGAAACTGGAAAATGAACGGTCTGATGGCGAATCTCGCCGAGGTCGAACGGGTCGCCGC
AGAGGTTCCCGCATCAAGTGAGGGGGCTGAAACGCTGTTATGCCTTCCTGCAACGTTGATTCACGCAGGTTCGGCAAAAT
CTGAAGGATCGGGCCTGAAAATTGGCGGAGAAACCTGCCACGCCAACGAAAAGGGCGCCCATACCGGCGATCTTGCAGCA
GAAATGCTCAAAGATGCCGGCGCCAGCTATGTCATTGTGGGGCACTCAGAGCGCCGCGCGGACCATGGCGAGACAGATGC
CGTAGTGGCTGCGCAGGCCTCGGCTGCATTGAGAGCAGGGATCACCCCAATCATCTGCGTTGGCGAAACCCTTGACCAAC
GTGATGCTGGGGAAGTGCTCACTGTCATCACGACGCAGATGGCAGAATCGATTCCCGAGGGTGCTGAGGCAGCCGCAATC
GTCATTGCTTATGAGCCGGTTTGGGCCATTGGGACCGGCCGTGTCGCCACTTCCGAGCAAATTGCCGAAGTGCACACGTC
GATCCGGAACCTTCTGGTGCGCCGATTTGGCGACGCAGGGCGCACGACCCGTATCCTTTATGGCGGTAGCATGAACCCCG
GAAATGCTGCCGAAATCCTCGCTGTGGCCGAGGTAAATGGCGGCCTGATTGGGGGGGCAAGCTTGAAGGCGGCGGACTTC
CTCGCCATTTACCGGTTGGCGGCGCAGTAG

Upstream 100 bases:

>100_bases
TAAGTGCGAGCATACGGCGCTTCTCTATTTCTGGGGTTTGCCGGGTGGCTCACAGCTTGCTAGGCGACCGGTTTATGACT
TGCAAGAGAGGGAAGGCCGC

Downstream 100 bases:

>100_bases
TTTGTGTGGAACCGCTGACTTGGCGAACGGCGTGATCGGGGTTATGCGGGCGCCTTAAATTCAGGAAAGCTGGTCGGGCC
ATGCAGAACGTCATTCTCGT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPRTLIAGNWKMNGLMANLAEVERVAAEVPASSEGAETLLCLPATLIHAGSAKSEGSGLKIGGETCHANEKGAHTGDLAA
EMLKDAGASYVIVGHSERRADHGETDAVVAAQASAALRAGITPIICVGETLDQRDAGEVLTVITTQMAESIPEGAEAAAI
VIAYEPVWAIGTGRVATSEQIAEVHTSIRNLLVRRFGDAGRTTRILYGGSMNPGNAAEILAVAEVNGGLIGGASLKAADF
LAIYRLAAQ

Sequences:

>Translated_249_residues
MPRTLIAGNWKMNGLMANLAEVERVAAEVPASSEGAETLLCLPATLIHAGSAKSEGSGLKIGGETCHANEKGAHTGDLAA
EMLKDAGASYVIVGHSERRADHGETDAVVAAQASAALRAGITPIICVGETLDQRDAGEVLTVITTQMAESIPEGAEAAAI
VIAYEPVWAIGTGRVATSEQIAEVHTSIRNLLVRRFGDAGRTTRILYGGSMNPGNAAEILAVAEVNGGLIGGASLKAADF
LAIYRLAAQ
>Mature_248_residues
PRTLIAGNWKMNGLMANLAEVERVAAEVPASSEGAETLLCLPATLIHAGSAKSEGSGLKIGGETCHANEKGAHTGDLAAE
MLKDAGASYVIVGHSERRADHGETDAVVAAQASAALRAGITPIICVGETLDQRDAGEVLTVITTQMAESIPEGAEAAAIV
IAYEPVWAIGTGRVATSEQIAEVHTSIRNLLVRRFGDAGRTTRILYGGSMNPGNAAEILAVAEVNGGLIGGASLKAADFL
AIYRLAAQ

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=244, Percent_Identity=38.1147540983607, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI226529917, Length=244, Percent_Identity=38.1147540983607, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=39.2, Blast_Score=171, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17536593, Length=243, Percent_Identity=40.7407407407407, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6320255, Length=240, Percent_Identity=40.8333333333333, Blast_Score=179, Evalue=5e-46,
Organism=Drosophila melanogaster, GI28572004, Length=243, Percent_Identity=40.3292181069959, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28572008, Length=242, Percent_Identity=39.2561983471074, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28572006, Length=242, Percent_Identity=39.2561983471074, Blast_Score=168, Evalue=3e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_HYPNA (Q0C197)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760496.1
- ProteinModelPortal:   Q0C197
- SMR:   Q0C197
- STRING:   Q0C197
- GeneID:   4288693
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1792
- NMPDR:   fig|228405.5.peg.1725
- TIGR:   HNE_1792
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   HSERANE
- BioCyc:   HNEP81032:HNE_1792-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25566; Mature: 25435

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTLIAGNWKMNGLMANLAEVERVAAEVPASSEGAETLLCLPATLIHAGSAKSEGSGLK
CCCCEEECCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCEE
IGGETCHANEKGAHTGDLAAEMLKDAGASYVIVGHSERRADHGETDAVVAAQASAALRAG
ECCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCCEEEEHHHHHHHHCC
ITPIICVGETLDQRDAGEVLTVITTQMAESIPEGAEAAAIVIAYEPVWAIGTGRVATSEQ
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEECCCCCCCHHH
IAEVHTSIRNLLVRRFGDAGRTTRILYGGSMNPGNAAEILAVAEVNGGLIGGASLKAADF
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCHHHH
LAIYRLAAQ
HHHHHHHCC
>Mature Secondary Structure 
PRTLIAGNWKMNGLMANLAEVERVAAEVPASSEGAETLLCLPATLIHAGSAKSEGSGLK
CCCEEECCCEECHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCEE
IGGETCHANEKGAHTGDLAAEMLKDAGASYVIVGHSERRADHGETDAVVAAQASAALRAG
ECCCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCCEEEEHHHHHHHHCC
ITPIICVGETLDQRDAGEVLTVITTQMAESIPEGAEAAAIVIAYEPVWAIGTGRVATSEQ
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEECCCCCCCHHH
IAEVHTSIRNLLVRRFGDAGRTTRILYGGSMNPGNAAEILAVAEVNGGLIGGASLKAADF
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCEECCCCCCHHHH
LAIYRLAAQ
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA