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The map label for this gene is nemA [C]

Identifier: 114799651

GI number: 114799651

Start: 3102708

End: 3103814

Strand: Reverse

Name: nemA [C]

Synonym: HNE_2942

Alternate gene names: 114799651

Gene position: 3103814-3102708 (Counterclockwise)

Preceding gene: 114800016

Following gene: 114797131

Centisome position: 83.77

GC content: 62.51

Gene sequence:

>1107_bases
ATGACCCGGACCCTGTTCACGCCTTTCCGGCTGAAAAACGTCGAACTTCCCAACCGTATCGTGATGGCGCCGATGACGCG
CTCCAAGTCTCCAGGCGGCGTTCCAGGCGAAGATGTGGCTGATTATTACGCCCGCCGTGCCGCCGCCGATGTGGGCCTCC
TGATCACCGAAGGCACCACGGTGCGCCGGGGTGGCTCGTCCAACGACCCCAATGTTCCCAATTTTTACAAGGCAGACGCG
CTCGATGGCTGGTCGCGGGTTGTCTCCAAGGTTCACGCCAATGGCGGCAAGATTGCCCCCCAGATCTGGCACCAGGGCAT
GGTGCGCAAGACCGGCACCGGGCCGGAGCCTGATGCGCCCACCGACAGCCCCTCGGGCGTCACCCATACCGGCAAACAGA
TTTTGCCAGAGCCGACCAGCGCCGATGTCGACGACATGGTGATGGCGTTTGCAGACGCGGCCGCTGAATCCAAGCGTCTC
GGCTTCGATACGCTGGAAATCCACGGCGCCCATGGCTACCTCGTCGACCAATTCTTCTGGGACGCGATGAACAAGCGCTC
CGACAAATATGGCGGCAGCCTCACGGACCGCGCGAGCTTTGGCGGCGACATCATCCGTGAAATCCGCAAGAAGGTCGGCC
CGGATCATCCGATCATCCTGCGCTATTCGCAGTGGAAGCAGCAGGACTATACCGCCCGCCTGGCGCCCACGCCGCAGGAG
CTGGAAGCGTTCCTCAAGGTGTTCGTGGATGCGGGCGTTGACTGTCTGCACGTTTCCCAGCGCCGCTATTGGGAGCCTGA
ATTCCCTGAGATCGACGGCGAACACGGCCTCAATGGCGCCGGCTGGGCAAAAAAGCTGACCGGCCTGCCCACCATCACGG
TCGGCTCGGTTGGCCTCAACGGCGATTTCGAGAAATCCTTCGCCGGGGAAGGCGCTCCGCGCCGTTCACTCGACGACCTG
GAAGCGCGTCTCGACAAGGGCGAGTTTGATCTCGTCGGCGTTGGCCGCGCGGTCCTTCAGGACCCGGAATGGGCCAAGAA
GGTCAAGGAAGGCCGGTTCAACGAGATTTCCGACTATAACGGCAAGGCGCTGGCGACGCTTTACTAG

Upstream 100 bases:

>100_bases
CGTCAGTTCAGGGGTGCCGCCCCTATACTGTAACGTCAATGGTTGACGCAGACCGGACACTTGCCCACTTCATCACCCGG
TAAACTCAAGGAAACCGCCA

Downstream 100 bases:

>100_bases
GCCGCCCGCCACTGCGAACACATCCAGTCTGGTCCGGCCTGCGTATCGGTTGAATAAGTTCAACCAGCAGACCGGACCAG
ATCTCATGCGTGTTGCCCGT

Product: FAD/FMN-binding oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTRTLFTPFRLKNVELPNRIVMAPMTRSKSPGGVPGEDVADYYARRAAADVGLLITEGTTVRRGGSSNDPNVPNFYKADA
LDGWSRVVSKVHANGGKIAPQIWHQGMVRKTGTGPEPDAPTDSPSGVTHTGKQILPEPTSADVDDMVMAFADAAAESKRL
GFDTLEIHGAHGYLVDQFFWDAMNKRSDKYGGSLTDRASFGGDIIREIRKKVGPDHPIILRYSQWKQQDYTARLAPTPQE
LEAFLKVFVDAGVDCLHVSQRRYWEPEFPEIDGEHGLNGAGWAKKLTGLPTITVGSVGLNGDFEKSFAGEGAPRRSLDDL
EARLDKGEFDLVGVGRAVLQDPEWAKKVKEGRFNEISDYNGKALATLY

Sequences:

>Translated_368_residues
MTRTLFTPFRLKNVELPNRIVMAPMTRSKSPGGVPGEDVADYYARRAAADVGLLITEGTTVRRGGSSNDPNVPNFYKADA
LDGWSRVVSKVHANGGKIAPQIWHQGMVRKTGTGPEPDAPTDSPSGVTHTGKQILPEPTSADVDDMVMAFADAAAESKRL
GFDTLEIHGAHGYLVDQFFWDAMNKRSDKYGGSLTDRASFGGDIIREIRKKVGPDHPIILRYSQWKQQDYTARLAPTPQE
LEAFLKVFVDAGVDCLHVSQRRYWEPEFPEIDGEHGLNGAGWAKKLTGLPTITVGSVGLNGDFEKSFAGEGAPRRSLDDL
EARLDKGEFDLVGVGRAVLQDPEWAKKVKEGRFNEISDYNGKALATLY
>Mature_367_residues
TRTLFTPFRLKNVELPNRIVMAPMTRSKSPGGVPGEDVADYYARRAAADVGLLITEGTTVRRGGSSNDPNVPNFYKADAL
DGWSRVVSKVHANGGKIAPQIWHQGMVRKTGTGPEPDAPTDSPSGVTHTGKQILPEPTSADVDDMVMAFADAAAESKRLG
FDTLEIHGAHGYLVDQFFWDAMNKRSDKYGGSLTDRASFGGDIIREIRKKVGPDHPIILRYSQWKQQDYTARLAPTPQEL
EAFLKVFVDAGVDCLHVSQRRYWEPEFPEIDGEHGLNGAGWAKKLTGLPTITVGSVGLNGDFEKSFAGEGAPRRSLDDLE
ARLDKGEFDLVGVGRAVLQDPEWAKKVKEGRFNEISDYNGKALATLY

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=26.8493150684932, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789463, Length=381, Percent_Identity=27.0341207349081, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=364, Percent_Identity=23.9010989010989, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17559804, Length=362, Percent_Identity=23.4806629834254, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=356, Percent_Identity=23.5955056179775, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17559802, Length=355, Percent_Identity=23.6619718309859, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540738, Length=212, Percent_Identity=26.8867924528302, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565138, Length=354, Percent_Identity=23.4463276836158, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=355, Percent_Identity=27.6056338028169, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325086, Length=370, Percent_Identity=24.3243243243243, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40348; Mature: 40217

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTLFTPFRLKNVELPNRIVMAPMTRSKSPGGVPGEDVADYYARRAAADVGLLITEGTT
CCCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCE
VRRGGSSNDPNVPNFYKADALDGWSRVVSKVHANGGKIAPQIWHQGMVRKTGTGPEPDAP
EECCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCC
TDSPSGVTHTGKQILPEPTSADVDDMVMAFADAAAESKRLGFDTLEIHGAHGYLVDQFFW
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
DAMNKRSDKYGGSLTDRASFGGDIIREIRKKVGPDHPIILRYSQWKQQDYTARLAPTPQE
HHHHHCCHHCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEECCCCHHH
LEAFLKVFVDAGVDCLHVSQRRYWEPEFPEIDGEHGLNGAGWAKKLTGLPTITVGSVGLN
HHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCC
GDFEKSFAGEGAPRRSLDDLEARLDKGEFDLVGVGRAVLQDPEWAKKVKEGRFNEISDYN
CCCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHCCCCCCCCCCC
GKALATLY
CCEEEEEC
>Mature Secondary Structure 
TRTLFTPFRLKNVELPNRIVMAPMTRSKSPGGVPGEDVADYYARRAAADVGLLITEGTT
CCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCE
VRRGGSSNDPNVPNFYKADALDGWSRVVSKVHANGGKIAPQIWHQGMVRKTGTGPEPDAP
EECCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCC
TDSPSGVTHTGKQILPEPTSADVDDMVMAFADAAAESKRLGFDTLEIHGAHGYLVDQFFW
CCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
DAMNKRSDKYGGSLTDRASFGGDIIREIRKKVGPDHPIILRYSQWKQQDYTARLAPTPQE
HHHHHCCHHCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEECCCCHHH
LEAFLKVFVDAGVDCLHVSQRRYWEPEFPEIDGEHGLNGAGWAKKLTGLPTITVGSVGLN
HHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCC
GDFEKSFAGEGAPRRSLDDLEARLDKGEFDLVGVGRAVLQDPEWAKKVKEGRFNEISDYN
CCCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHHHCCCCCCCCCCC
GKALATLY
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]