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The map label for this gene is alsS [H]

Identifier: 114799618

GI number: 114799618

Start: 2630752

End: 2632407

Strand: Reverse

Name: alsS [H]

Synonym: HNE_2501

Alternate gene names: 114799618

Gene position: 2632407-2630752 (Counterclockwise)

Preceding gene: 114800001

Following gene: 114799601

Centisome position: 71.05

GC content: 61.35

Gene sequence:

>1656_bases
ATGCCGAAGGGGTCGGACCTGTTCGTTGCCGCGCTCGAAAACGAAGGCGTGGACTATATCTTCGGCGTGCCCGGGGAGGA
AAACCTCGACTTTCTGGAGTCCCTGCGGACCTCCAGAATTCGGCTGATCCTGACCCGTCATGAGCAGGCGGCCGCCTTCA
TGGCGGCGACACATGGGCGCCTGACGGGCAGGCCGGGCGTCTGCCTCGCCACCCTTGGGCCGGGCGCGCTGAATATGGCA
ACGGGGGCCGCCTATGCGCATCTTGGCGCTATGCCGATGATCCTGATCACGGGCCAGAAGCCGGTGAAGAGCGCCCGGCA
GGCGAGATTTCAGATCGTCGATATAGTCGCGTCGATGAAGCCGCTCACCAAGATGTCGCGCCAGATAATCAGTGCGGGGA
GCATTCCGGCAATCGTAAGGGATGCGTTCCGCACGGCCATGGAGGAGCGGCCCGGACCGGTCCACCTTGAACTGCCCGAA
GATGTCGCGTCGGAGGAAACAGGCGGCGCGTTTATGATCCCGGTTCATGACATTGCGCGCCCCATCGCCAGCCCGTCAGA
ACTGGATAAAGCCGCCGATGTGATCCTCGCCGCGGAACACCCTCTGGTAATGATCGGCGCGGCCGGAAACCGGCCCTGTC
TGGTGGAGCCGCTTTCGGCGTTCATCCGGCGCACCCGGATTCCCTTCTTCAACACCCAAATGGGCAAGGGTGCTGTTACG
GGGGGCTCCAGCCTTTTCATGGGAACAGCGGCGCTTTCGGAAGGCGACTATGTTCATGAGGCGGTCGACCGCGCCGACCT
GATCATCGCCATCGGCCATGACACGGTCGAGAAGCCACCATTCCTGATGAAGAACGCGGGGGGCCAGACGGTCCTCCATA
TTGGTTATCATTCGGCAGATGTAGAGGAGGTCTACCATCCTGACTTCGAAGTGATCGGCGATATCGGGGCAACGGTCGAA
GGGCTCGGGGAAAGGCTGGAAGGCCGGCTGCAGCCTGATCAGGGCATGCTGGATCTGCGCCAGAAAATCCTCGCGCATCT
AAACGACCGCGCCGAAGAAGACCGCTTTCCGATCACGCCGCAGCGTATCGTTCATGATGTCCGCAAGGTCATGCCGGAGG
ACGGCATCGTCTGCCTCGATAATGGCATGTACAAGATCTGGTTCGCCCGCAATTACCGCACGCACGTCGCCAACACGCTG
CTGCTCGACAACGCGCTGGCCACGATGGGCGCGGGGCTGCCATCTGCAATGATGGCCGCGATGCTCTATCCCGGCAGGCG
GGTCCTAGCGGTCTGTGGTGACGGCGGTTTCATGATGAACTCGCAGGAGATGGAAACGGCGGTGCGCCTTAGACTGAACC
TTGTCGTGATGATCCTCAATGACAGCGCCTATGGCATGATCCGCTGGAAGCAGGCCGTCGACGGCTTTGCCGATTTCGGC
ATGCGTTTTGGAAATCCTGACTTCGTACATTACGCAGAAGCCTATGGTGCCATGGGAACACGGGTAACCTCCGTGGCGGA
CCTGGCGCCGGCGCTCGAAGCGGCGTTCCTGGGAGGCGGCGTCCATCTAGTTGATGTGGCGATCGACTATTCCGAGAATA
CCCGGGTGCTCGTGGAGGAACTGCGCAACCGCAAGCCGGATGTGGATTGTGCGTGA

Upstream 100 bases:

>100_bases
AACGGGCCTGACCTGATCATGGTGGTTTAGCGTGGCGGGATATGTGTCTTCTCTTGATGAGCGGGCGCTCTCAAGAGGTC
CTCGATAAGGAGTTAATGCG

Downstream 100 bases:

>100_bases
GCGGATCTACTCTGAGAGGCCTTATACCTGGAGAGGTTGATGATGTTGAAAGTCCTGCAGGCTTATGACCGCGCCCTGAT
CACTGAAATTGAGACGGACG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MPKGSDLFVAALENEGVDYIFGVPGEENLDFLESLRTSRIRLILTRHEQAAAFMAATHGRLTGRPGVCLATLGPGALNMA
TGAAYAHLGAMPMILITGQKPVKSARQARFQIVDIVASMKPLTKMSRQIISAGSIPAIVRDAFRTAMEERPGPVHLELPE
DVASEETGGAFMIPVHDIARPIASPSELDKAADVILAAEHPLVMIGAAGNRPCLVEPLSAFIRRTRIPFFNTQMGKGAVT
GGSSLFMGTAALSEGDYVHEAVDRADLIIAIGHDTVEKPPFLMKNAGGQTVLHIGYHSADVEEVYHPDFEVIGDIGATVE
GLGERLEGRLQPDQGMLDLRQKILAHLNDRAEEDRFPITPQRIVHDVRKVMPEDGIVCLDNGMYKIWFARNYRTHVANTL
LLDNALATMGAGLPSAMMAAMLYPGRRVLAVCGDGGFMMNSQEMETAVRLRLNLVVMILNDSAYGMIRWKQAVDGFADFG
MRFGNPDFVHYAEAYGAMGTRVTSVADLAPALEAAFLGGGVHLVDVAIDYSENTRVLVEELRNRKPDVDCA

Sequences:

>Translated_551_residues
MPKGSDLFVAALENEGVDYIFGVPGEENLDFLESLRTSRIRLILTRHEQAAAFMAATHGRLTGRPGVCLATLGPGALNMA
TGAAYAHLGAMPMILITGQKPVKSARQARFQIVDIVASMKPLTKMSRQIISAGSIPAIVRDAFRTAMEERPGPVHLELPE
DVASEETGGAFMIPVHDIARPIASPSELDKAADVILAAEHPLVMIGAAGNRPCLVEPLSAFIRRTRIPFFNTQMGKGAVT
GGSSLFMGTAALSEGDYVHEAVDRADLIIAIGHDTVEKPPFLMKNAGGQTVLHIGYHSADVEEVYHPDFEVIGDIGATVE
GLGERLEGRLQPDQGMLDLRQKILAHLNDRAEEDRFPITPQRIVHDVRKVMPEDGIVCLDNGMYKIWFARNYRTHVANTL
LLDNALATMGAGLPSAMMAAMLYPGRRVLAVCGDGGFMMNSQEMETAVRLRLNLVVMILNDSAYGMIRWKQAVDGFADFG
MRFGNPDFVHYAEAYGAMGTRVTSVADLAPALEAAFLGGGVHLVDVAIDYSENTRVLVEELRNRKPDVDCA
>Mature_550_residues
PKGSDLFVAALENEGVDYIFGVPGEENLDFLESLRTSRIRLILTRHEQAAAFMAATHGRLTGRPGVCLATLGPGALNMAT
GAAYAHLGAMPMILITGQKPVKSARQARFQIVDIVASMKPLTKMSRQIISAGSIPAIVRDAFRTAMEERPGPVHLELPED
VASEETGGAFMIPVHDIARPIASPSELDKAADVILAAEHPLVMIGAAGNRPCLVEPLSAFIRRTRIPFFNTQMGKGAVTG
GSSLFMGTAALSEGDYVHEAVDRADLIIAIGHDTVEKPPFLMKNAGGQTVLHIGYHSADVEEVYHPDFEVIGDIGATVEG
LGERLEGRLQPDQGMLDLRQKILAHLNDRAEEDRFPITPQRIVHDVRKVMPEDGIVCLDNGMYKIWFARNYRTHVANTLL
LDNALATMGAGLPSAMMAAMLYPGRRVLAVCGDGGFMMNSQEMETAVRLRLNLVVMILNDSAYGMIRWKQAVDGFADFGM
RFGNPDFVHYAEAYGAMGTRVTSVADLAPALEAAFLGGGVHLVDVAIDYSENTRVLVEELRNRKPDVDCA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=541, Percent_Identity=24.7689463955638, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=24.0437158469945, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI87081685, Length=551, Percent_Identity=29.038112522686, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=28.6231884057971, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=27.7985074626866, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787096, Length=529, Percent_Identity=26.4650283553875, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=25.9786476868327, Blast_Score=129, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=541, Percent_Identity=27.5415896487985, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=541, Percent_Identity=27.5415896487985, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=546, Percent_Identity=21.978021978022, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=27.0650263620387, Blast_Score=191, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=535, Percent_Identity=23.9252336448598, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=24.4939271255061, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=23.6472945891784, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=492, Percent_Identity=23.9837398373984, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=27.6288659793814, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59700; Mature: 59568

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKGSDLFVAALENEGVDYIFGVPGEENLDFLESLRTSRIRLILTRHEQAAAFMAATHGR
CCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCEEEEEEEECHHHHHHHHHHCCC
LTGRPGVCLATLGPGALNMATGAAYAHLGAMPMILITGQKPVKSARQARFQIVDIVASMK
CCCCCCEEEEECCCCCHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
PLTKMSRQIISAGSIPAIVRDAFRTAMEERPGPVHLELPEDVASEETGGAFMIPVHDIAR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCEEEEEHHHHHH
PIASPSELDKAADVILAAEHPLVMIGAAGNRPCLVEPLSAFIRRTRIPFFNTQMGKGAVT
CCCCHHHHHHHHCEEEECCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEECCCCCCEEC
GGSSLFMGTAALSEGDYVHEAVDRADLIIAIGHDTVEKPPFLMKNAGGQTVLHIGYHSAD
CCCCEEEEEHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCEEEEEECCCCC
VEEVYHPDFEVIGDIGATVEGLGERLEGRLQPDQGMLDLRQKILAHLNDRAEEDRFPITP
HHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
QRIVHDVRKVMPEDGIVCLDNGMYKIWFARNYRTHVANTLLLDNALATMGAGLPSAMMAA
HHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
MLYPGRRVLAVCGDGGFMMNSQEMETAVRLRLNLVVMILNDSAYGMIRWKQAVDGFADFG
HHCCCCEEEEEECCCCEEECHHHHHHHHHEEEEEEEEEEECCCCEEEEEHHHHHHHHHHH
MRFGNPDFVHYAEAYGAMGTRVTSVADLAPALEAAFLGGGVHLVDVAIDYSENTRVLVEE
HCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHH
LRNRKPDVDCA
HHCCCCCCCCC
>Mature Secondary Structure 
PKGSDLFVAALENEGVDYIFGVPGEENLDFLESLRTSRIRLILTRHEQAAAFMAATHGR
CCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHCEEEEEEEECHHHHHHHHHHCCC
LTGRPGVCLATLGPGALNMATGAAYAHLGAMPMILITGQKPVKSARQARFQIVDIVASMK
CCCCCCEEEEECCCCCHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
PLTKMSRQIISAGSIPAIVRDAFRTAMEERPGPVHLELPEDVASEETGGAFMIPVHDIAR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHCCCCCCCEEEEEHHHHHH
PIASPSELDKAADVILAAEHPLVMIGAAGNRPCLVEPLSAFIRRTRIPFFNTQMGKGAVT
CCCCHHHHHHHHCEEEECCCCEEEEECCCCCCEEHHHHHHHHHHHCCCCEECCCCCCEEC
GGSSLFMGTAALSEGDYVHEAVDRADLIIAIGHDTVEKPPFLMKNAGGQTVLHIGYHSAD
CCCCEEEEEHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCEEEEEECCCCC
VEEVYHPDFEVIGDIGATVEGLGERLEGRLQPDQGMLDLRQKILAHLNDRAEEDRFPITP
HHHHHCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
QRIVHDVRKVMPEDGIVCLDNGMYKIWFARNYRTHVANTLLLDNALATMGAGLPSAMMAA
HHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
MLYPGRRVLAVCGDGGFMMNSQEMETAVRLRLNLVVMILNDSAYGMIRWKQAVDGFADFG
HHCCCCEEEEEECCCCEEECHHHHHHHHHEEEEEEEEEEECCCCEEEEEHHHHHHHHHHH
MRFGNPDFVHYAEAYGAMGTRVTSVADLAPALEAAFLGGGVHLVDVAIDYSENTRVLVEE
HCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHH
LRNRKPDVDCA
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]