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The map label for this gene is camA [H]

Identifier: 114799596

GI number: 114799596

Start: 1954986

End: 1956230

Strand: Direct

Name: camA [H]

Synonym: HNE_1888

Alternate gene names: 114799596

Gene position: 1954986-1956230 (Clockwise)

Preceding gene: 114797217

Following gene: 114800377

Centisome position: 52.77

GC content: 61.93

Gene sequence:

>1245_bases
GTGGACCTTTCGGGCGCAAAACGGATTGTAATTGTCGGCGCTGGCCAGGCGGCTGCGCAGGCTGTTCAATCCTTGCGCCT
GGGTGGTTATGCTGGCGAGCTCACAATCGTCGGTGAAGAGACGGCGCTTCCCTATCAGCGTCCCCCACTCTCGAAGGCTT
ACATGAAGGGCGAGATGGCGGAGGAGCGCCTCTATTTCCGCCCCGCGGCCTGGTACGAAGACAACAAGATCGAAGTGATG
CTGGGCTCCCGCGTCACCTCCATTGATCGCGCCGCGCGCGTCGCCCATCTCGAACATGGCGCCGAACTGCCTTACGACGC
GCTGGTCATCGCCACAGGTTCCCGCCCCCGCACGCTTCCCTGCCAGGGCGCTGATTTGACCGGCGTTCACGATCTGCGCA
GCCTGTCGGATGTCGAACGCATCCGCCCGCAAATGGTCGAAGGCCGCCGCATGGTCATAATCGGCGCCGGCTATATCGGC
CTTGAAGCGGCAGCTGTCGCACGCACCATGGGTCTGGATGTCACTGTTCTGGAAATGGCACCGCGCGTTCTGGCGCGCGT
TACAAGCCCTGTAATGAGCGAATTCTACGCCGCCGAGCACATCGCCAAAGGCGTCAAGATCCTCACCAGCACGGCCCTGT
CGCATCTCGAAGGCAAGGACGGCCATATCAACGCCGCCGCGCTGGCCGATGGCACGAAGCTCCCGGCCGACATCGTCCTC
GTCGGCATCGGCATCCTGCCCAATGAAGAGCTTGCAAAAGACGCCGGCATCGCCTGTTCCAACGGCATCCTGACCGACCG
GGACGGCCGCACATCCGACCCGCACGTGTTTGCGGCCGGTGACTGCGCCAGCCGTCCGCTGGTTCACTATGGCCGTACAG
GGCGCCTCGAAAGCGTCCACAACGCCATCGAGCAGGGCAAGCTCGTCGCCGCTGCCATTCTTGGACAAAATCGGCCGGCA
GAGGACTGCCCCTGGTTCTGGTCGGACCAATATGACCTCAAACTCCAGATCGCCGGACTCAGTACGGACTATGACACGAT
CGTCCTGCGCGGCGATCCTGAGGACCGCAAGTTTGCCGCTTTCTATCTGCGCAATGGCACCCTGATCGCCGTTGACGCCG
TCAACAGCCCGCCAGAGTTCCTGGCATCCAAGAAACTCATCATGACAGGCGCAAAAATTGCCCCTGAAGTGCTCAGCGAC
ACCTCAATCCCAATGAAAGACATCGCCGCAAAAGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
ACGGAGTGACCCTGCTTGCACTGCTTGCGGGCTGCGCTAGAAGTGCTTAGAACCAGACAAAAGTTGACGCTTGCGTCATA
AATCATATCAAGGGGGCGTC

Downstream 100 bases:

>100_bases
AGGAGACGGAAATCCACATGGCAAAGATCACCTATGTTTCGCATGACGGCACCGAACGCACGGTGGAAGCGAAGAACGGC
GAATCTGTAATGGAAGCGGC

Product: pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDLSGAKRIVIVGAGQAAAQAVQSLRLGGYAGELTIVGEETALPYQRPPLSKAYMKGEMAEERLYFRPAAWYEDNKIEVM
LGSRVTSIDRAARVAHLEHGAELPYDALVIATGSRPRTLPCQGADLTGVHDLRSLSDVERIRPQMVEGRRMVIIGAGYIG
LEAAAVARTMGLDVTVLEMAPRVLARVTSPVMSEFYAAEHIAKGVKILTSTALSHLEGKDGHINAAALADGTKLPADIVL
VGIGILPNEELAKDAGIACSNGILTDRDGRTSDPHVFAAGDCASRPLVHYGRTGRLESVHNAIEQGKLVAAAILGQNRPA
EDCPWFWSDQYDLKLQIAGLSTDYDTIVLRGDPEDRKFAAFYLRNGTLIAVDAVNSPPEFLASKKLIMTGAKIAPEVLSD
TSIPMKDIAAKAAA

Sequences:

>Translated_414_residues
MDLSGAKRIVIVGAGQAAAQAVQSLRLGGYAGELTIVGEETALPYQRPPLSKAYMKGEMAEERLYFRPAAWYEDNKIEVM
LGSRVTSIDRAARVAHLEHGAELPYDALVIATGSRPRTLPCQGADLTGVHDLRSLSDVERIRPQMVEGRRMVIIGAGYIG
LEAAAVARTMGLDVTVLEMAPRVLARVTSPVMSEFYAAEHIAKGVKILTSTALSHLEGKDGHINAAALADGTKLPADIVL
VGIGILPNEELAKDAGIACSNGILTDRDGRTSDPHVFAAGDCASRPLVHYGRTGRLESVHNAIEQGKLVAAAILGQNRPA
EDCPWFWSDQYDLKLQIAGLSTDYDTIVLRGDPEDRKFAAFYLRNGTLIAVDAVNSPPEFLASKKLIMTGAKIAPEVLSD
TSIPMKDIAAKAAA
>Mature_414_residues
MDLSGAKRIVIVGAGQAAAQAVQSLRLGGYAGELTIVGEETALPYQRPPLSKAYMKGEMAEERLYFRPAAWYEDNKIEVM
LGSRVTSIDRAARVAHLEHGAELPYDALVIATGSRPRTLPCQGADLTGVHDLRSLSDVERIRPQMVEGRRMVIIGAGYIG
LEAAAVARTMGLDVTVLEMAPRVLARVTSPVMSEFYAAEHIAKGVKILTSTALSHLEGKDGHINAAALADGTKLPADIVL
VGIGILPNEELAKDAGIACSNGILTDRDGRTSDPHVFAAGDCASRPLVHYGRTGRLESVHNAIEQGKLVAAAILGQNRPA
EDCPWFWSDQYDLKLQIAGLSTDYDTIVLRGDPEDRKFAAFYLRNGTLIAVDAVNSPPEFLASKKLIMTGAKIAPEVLSD
TSIPMKDIAAKAAA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=393, Percent_Identity=29.7709923664122, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI226437568, Length=393, Percent_Identity=29.7709923664122, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI65787454, Length=393, Percent_Identity=29.7709923664122, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI22202629, Length=346, Percent_Identity=26.0115606936416, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI4757732, Length=346, Percent_Identity=26.0115606936416, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI195927006, Length=176, Percent_Identity=27.8409090909091, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=39.5522388059701, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789065, Length=312, Percent_Identity=29.1666666666667, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789765, Length=283, Percent_Identity=30.7420494699647, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786307, Length=225, Percent_Identity=28, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082354, Length=255, Percent_Identity=28.6274509803922, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=378, Percent_Identity=26.984126984127, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24639257, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281359715, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281359713, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639250, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI18543267, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639252, Length=389, Percent_Identity=28.2776349614396, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24585130, Length=372, Percent_Identity=26.3440860215054, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28573993, Length=372, Percent_Identity=26.0752688172043, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24581020, Length=372, Percent_Identity=26.0752688172043, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 44260; Mature: 44260

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSGAKRIVIVGAGQAAAQAVQSLRLGGYAGELTIVGEETALPYQRPPLSKAYMKGEMA
CCCCCCCEEEEEECCHHHHHHHHHHHCCCEECEEEEEECCCCCCCCCCCCHHHHHHCHHH
EERLYFRPAAWYEDNKIEVMLGSRVTSIDRAARVAHLEHGAELPYDALVIATGSRPRTLP
HHHEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
CQGADLTGVHDLRSLSDVERIRPQMVEGRRMVIIGAGYIGLEAAAVARTMGLDVTVLEMA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEHHHHH
PRVLARVTSPVMSEFYAAEHIAKGVKILTSTALSHLEGKDGHINAAALADGTKLPADIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
VGIGILPNEELAKDAGIACSNGILTDRDGRTSDPHVFAAGDCASRPLVHYGRTGRLESVH
EEECCCCCHHHHHHCCCEECCCEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCHHHHH
NAIEQGKLVAAAILGQNRPAEDCPWFWSDQYDLKLQIAGLSTDYDTIVLRGDPEDRKFAA
HHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEE
FYLRNGTLIAVDAVNSPPEFLASKKLIMTGAKIAPEVLSDTSIPMKDIAAKAAA
EEEECCCEEEEECCCCCHHHHHCCHHEEECCHHCHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MDLSGAKRIVIVGAGQAAAQAVQSLRLGGYAGELTIVGEETALPYQRPPLSKAYMKGEMA
CCCCCCCEEEEEECCHHHHHHHHHHHCCCEECEEEEEECCCCCCCCCCCCHHHHHHCHHH
EERLYFRPAAWYEDNKIEVMLGSRVTSIDRAARVAHLEHGAELPYDALVIATGSRPRTLP
HHHEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
CQGADLTGVHDLRSLSDVERIRPQMVEGRRMVIIGAGYIGLEAAAVARTMGLDVTVLEMA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEHHHHH
PRVLARVTSPVMSEFYAAEHIAKGVKILTSTALSHLEGKDGHINAAALADGTKLPADIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEE
VGIGILPNEELAKDAGIACSNGILTDRDGRTSDPHVFAAGDCASRPLVHYGRTGRLESVH
EEECCCCCHHHHHHCCCEECCCEEECCCCCCCCCEEEEECCCCCCCCEECCCCCCHHHHH
NAIEQGKLVAAAILGQNRPAEDCPWFWSDQYDLKLQIAGLSTDYDTIVLRGDPEDRKFAA
HHHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEECCCCCCEEEE
FYLRNGTLIAVDAVNSPPEFLASKKLIMTGAKIAPEVLSDTSIPMKDIAAKAAA
EEEECCCEEEEECCCCCHHHHHCCHHEEECCHHCHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]