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The map label for this gene is ispH

Identifier: 114799593

GI number: 114799593

Start: 2874605

End: 2875552

Strand: Reverse

Name: ispH

Synonym: HNE_2713

Alternate gene names: 114799593

Gene position: 2875552-2874605 (Counterclockwise)

Preceding gene: 114798303

Following gene: 114799136

Centisome position: 77.61

GC content: 64.98

Gene sequence:

>948_bases
ATGACGACGAAACCCCCGCTTACCCTCCGCCTGGCCGCCCCGCGCGGCTTCTGCGCAGGCGTGGACCGCGCCATCCAGAT
CGTCGAGGAGGCCCTGAAAAAGTGGGGCGCCCCGGTCTATGTGCGCCATGAAATCGTCCACAACCGGCATGTGGTGGAGC
GTCTGGAAGCCCTCGGCGCCATCTTCGTGGAGGAGCTGGAAGAATGCCCCGATGACCGCCCGGTCATCTTCTCCGCCCAT
GGCGTGCCCAAATCCGTCCCCGCCGAGGCCGGCCGCCGCAACATGATCTATGTCGACGCGACCTGCCCGCTGGTCTCCAA
GGTGCATGTCGAGGCCGAGCGCCATTTCAACGCCGCGCGCGAGATTGTCCTCATCGGCCATGCCGGCCACCCGGAAGTCA
TCGGAACAATGGGCCAGCTCCCCGAAGGCTCCGTCGCCCTTATCGAGACCGTGGAGGATGCGCGCGCGTTCCAGCCCCGC
GACCACGCCAACCTCGCCTTCATCACGCAGACAACCTTGAGCGTAGACGACACGGCAGACATAGTCGCCGCCCTCCAGTC
CCGCTTCCCCGGCATCGCCACGCCGCACAAGGAAGACATCTGCTACGCCACCACCAATCGTCAGGAGGCGGTCAAAGTCT
TTGCGCCGGGCGCAGAGCTTGTTCTGGTGATCGGTGCAAGGACGAGTTCAAACTCCGTCCGCCTCGTCGAAGTCGCCCTC
CGCGCCGGCGCCCAAGACGCCAAGCTCATCGCCAGCGCCGACGACATAGACTGGTCCTGGTTCAACGGCATCACCACCCT
CGGCCTCACCGCCGGCGCCTCCGCCCCCGAAGACCTCGTCCAAGGCGTCATCGAAGCCTGCCGCGACCGCTTCGAGGTAA
CGGTGGAAACCGTGAAGACGGCGGATGAAACCGTGACGTTTAAATTGCCAAGAGTGCTGGCGGGATAA

Upstream 100 bases:

>100_bases
TCTTGGCCTTGTGTAAAGGCCTGTCCATCTAATAAGCCTGCCCGTGTTAAAGGCCTTGCGGGCGCCGCGCAACGCATTCG
GCGCAGGAGAAATACCCCAG

Downstream 100 bases:

>100_bases
ATAAAAAATACCGCACACGCGGTGGCAGCAAGTTCAAGACTGTAAACATGACCGATAAAGACCCCAGCCCATCAGATGAG
GCAGAAACCATCTCCGCGGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTTKPPLTLRLAAPRGFCAGVDRAIQIVEEALKKWGAPVYVRHEIVHNRHVVERLEALGAIFVEELEECPDDRPVIFSAH
GVPKSVPAEAGRRNMIYVDATCPLVSKVHVEAERHFNAAREIVLIGHAGHPEVIGTMGQLPEGSVALIETVEDARAFQPR
DHANLAFITQTTLSVDDTADIVAALQSRFPGIATPHKEDICYATTNRQEAVKVFAPGAELVLVIGARTSSNSVRLVEVAL
RAGAQDAKLIASADDIDWSWFNGITTLGLTAGASAPEDLVQGVIEACRDRFEVTVETVKTADETVTFKLPRVLAG

Sequences:

>Translated_315_residues
MTTKPPLTLRLAAPRGFCAGVDRAIQIVEEALKKWGAPVYVRHEIVHNRHVVERLEALGAIFVEELEECPDDRPVIFSAH
GVPKSVPAEAGRRNMIYVDATCPLVSKVHVEAERHFNAAREIVLIGHAGHPEVIGTMGQLPEGSVALIETVEDARAFQPR
DHANLAFITQTTLSVDDTADIVAALQSRFPGIATPHKEDICYATTNRQEAVKVFAPGAELVLVIGARTSSNSVRLVEVAL
RAGAQDAKLIASADDIDWSWFNGITTLGLTAGASAPEDLVQGVIEACRDRFEVTVETVKTADETVTFKLPRVLAG
>Mature_314_residues
TTKPPLTLRLAAPRGFCAGVDRAIQIVEEALKKWGAPVYVRHEIVHNRHVVERLEALGAIFVEELEECPDDRPVIFSAHG
VPKSVPAEAGRRNMIYVDATCPLVSKVHVEAERHFNAAREIVLIGHAGHPEVIGTMGQLPEGSVALIETVEDARAFQPRD
HANLAFITQTTLSVDDTADIVAALQSRFPGIATPHKEDICYATTNRQEAVKVFAPGAELVLVIGARTSSNSVRLVEVALR
AGAQDAKLIASADDIDWSWFNGITTLGLTAGASAPEDLVQGVIEACRDRFEVTVETVKTADETVTFKLPRVLAG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=52.1035598705502, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_HYPNA (Q0BYQ0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761393.1
- STRING:   Q0BYQ0
- GeneID:   4289089
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2713
- NMPDR:   fig|228405.5.peg.2600
- TIGR:   HNE_2713
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- PhylomeDB:   Q0BYQ0
- BioCyc:   HNEP81032:HNE_2713-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34047; Mature: 33916

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKPPLTLRLAAPRGFCAGVDRAIQIVEEALKKWGAPVYVRHEIVHNRHVVERLEALGA
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
IFVEELEECPDDRPVIFSAHGVPKSVPAEAGRRNMIYVDATCPLVSKVHVEAERHFNAAR
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
EIVLIGHAGHPEVIGTMGQLPEGSVALIETVEDARAFQPRDHANLAFITQTTLSVDDTAD
EEEEEECCCCCHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEEECCCCHHH
IVAALQSRFPGIATPHKEDICYATTNRQEAVKVFAPGAELVLVIGARTSSNSVRLVEVAL
HHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEHH
RAGAQDAKLIASADDIDWSWFNGITTLGLTAGASAPEDLVQGVIEACRDRFEVTVETVKT
HCCCCCCEEEECCCCCCCHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHHHC
ADETVTFKLPRVLAG
CCCEEEEECCHHHCC
>Mature Secondary Structure 
TTKPPLTLRLAAPRGFCAGVDRAIQIVEEALKKWGAPVYVRHEIVHNRHVVERLEALGA
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHH
IFVEELEECPDDRPVIFSAHGVPKSVPAEAGRRNMIYVDATCPLVSKVHVEAERHFNAAR
HHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCC
EIVLIGHAGHPEVIGTMGQLPEGSVALIETVEDARAFQPRDHANLAFITQTTLSVDDTAD
EEEEEECCCCCHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEEECCCCHHH
IVAALQSRFPGIATPHKEDICYATTNRQEAVKVFAPGAELVLVIGARTSSNSVRLVEVAL
HHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEEEEEHH
RAGAQDAKLIASADDIDWSWFNGITTLGLTAGASAPEDLVQGVIEACRDRFEVTVETVKT
HCCCCCCEEEECCCCCCCHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHEEHHHHHC
ADETVTFKLPRVLAG
CCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA