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The map label for this gene is tuf2 [H]

Identifier: 114799428

GI number: 114799428

Start: 3004398

End: 3005588

Strand: Reverse

Name: tuf2 [H]

Synonym: HNE_2853

Alternate gene names: 114799428

Gene position: 3005588-3004398 (Counterclockwise)

Preceding gene: 114800238

Following gene: 114798629

Centisome position: 81.12

GC content: 62.05

Gene sequence:

>1191_bases
ATGGGCAAGGCAAAGTTTGAGCGCAACAAGCCGCACGTAAACATTGGCACGATTGGCCACGTTGACCACGGCAAGACGAC
GCTGACAGCGGCGATCACGATCACGCTGGCGAAGACCGGTGGCGCGACGGCGAAGAATTACGCCGACATTGACGCGGCGC
CGGAAGAGAAGGCACGCGGGATCACGATCAACACGGCGCACGTCGAGTATGAGACGCCTGCCCGTCACTATGCCCACGTC
GACTGCCCCGGCCACGCTGACTATGTGAAGAACATGATCACCGGCGCTGCGCAGATGGACGGCGCGATCCTGGTGTGTTC
GGCCGCTGACGGCCCGATGCCGCAGACGCGTGAGCACATCCTTCTGGCCCGCCAGGTTGGCGTGCCGGCGCTGGTGGTGT
TCCTCAACAAGGTCGACATGGTCGACGATGAGGAGCTTCTGGAGCTGGTCGAGATGGAAGTGCGCGAGCTGCTTTCGTCC
TACAACTTCCCTGGCGACGACATTCCGATCATCAAGGGCTCGGCTCTGGCCGCTGTTGAAGACCGCAATCCTGAAATCGG
CCAGGACCGTATCCTGGAGCTGATGAAGGCTGTGGACGAGTACATCCCGACGCCGGAGCGTCCGCTGGACAAGCCGTTCC
TGATGCCGGTTGAAGACGTGTTCTCGATCTCGGGCCGCGGTACGGTTGTGACCGGCCGGGTTGAGCAGGGCATCGTCAAG
GTTGGCGAAGAAATCGAGATCGTCGGCATCCGCCCGACGGTGAAGACGACCTGCACGGGCGTTGAGATGTTCCGCAAGCT
GCTCGACCAGGGCCAGGCTGGCGACAACATCGGCGCGCTGCTGCGCGGTGTTGACCGTGAAGGCGTTGAGCGCGGGCAGG
TTCTCTGCAAGCCGGGCTCGATCACGCCGCACACGCTGTTCGAAGCCGAAGCCTACATCCTGACGAAGGAAGAGGGTGGC
CGTCACACGCCGTTCTTCACCAACTACCGTCCGCAGTTCTACTTCCGCACCACGGACGTCACAGGCATCGTCAAGCTGCC
TGAGGACAAAGAGATGGTTCTGCCGGGCGACAACGTGAAAATGGATGTCGAGCTGATCAACCCGATCGCCATGGACAAGG
GCCTGCGCTTCGCCATCCGCGAAGGCGGCCGCACCGTCGGCGCCGGCGTCGTCTCCGAAATCAAGAAATAA

Upstream 100 bases:

>100_bases
TCACTATGATGAAGTTCCGCGCGCTGAAGCACAGAAGATCATTCAGGAAGTCTCGGGCTCCTAATCGGGCCCCAAGTATA
AGAGCTAGAGGAGAGGCCCG

Downstream 100 bases:

>100_bases
GCTTCCCTCACAGGAAGTCCGAATCGAAAAGGCCGCCCCTCAAGGGCGGCCTTTTTGCTGTTATGGCGGGCATATCCGCC
CACTGCGCCCGCGCGGGGGA

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGKAKFERNKPHVNIGTIGHVDHGKTTLTAAITITLAKTGGATAKNYADIDAAPEEKARGITINTAHVEYETPARHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVEMEVRELLSS
YNFPGDDIPIIKGSALAAVEDRNPEIGQDRILELMKAVDEYIPTPERPLDKPFLMPVEDVFSISGRGTVVTGRVEQGIVK
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLCKPGSITPHTLFEAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIVKLPEDKEMVLPGDNVKMDVELINPIAMDKGLRFAIREGGRTVGAGVVSEIKK

Sequences:

>Translated_396_residues
MGKAKFERNKPHVNIGTIGHVDHGKTTLTAAITITLAKTGGATAKNYADIDAAPEEKARGITINTAHVEYETPARHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVEMEVRELLSS
YNFPGDDIPIIKGSALAAVEDRNPEIGQDRILELMKAVDEYIPTPERPLDKPFLMPVEDVFSISGRGTVVTGRVEQGIVK
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLCKPGSITPHTLFEAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGIVKLPEDKEMVLPGDNVKMDVELINPIAMDKGLRFAIREGGRTVGAGVVSEIKK
>Mature_395_residues
GKAKFERNKPHVNIGTIGHVDHGKTTLTAAITITLAKTGGATAKNYADIDAAPEEKARGITINTAHVEYETPARHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPALVVFLNKVDMVDDEELLELVEMEVRELLSSY
NFPGDDIPIIKGSALAAVEDRNPEIGQDRILELMKAVDEYIPTPERPLDKPFLMPVEDVFSISGRGTVVTGRVEQGIVKV
GEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLCKPGSITPHTLFEAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGIVKLPEDKEMVLPGDNVKMDVELINPIAMDKGLRFAIREGGRTVGAGVVSEIKK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=58.0562659846547, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=29.8876404494382, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=29.8876404494382, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=28.4444444444444, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=27.1111111111111, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI54607086, Length=326, Percent_Identity=28.5276073619632, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=28.955223880597, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=29.5031055900621, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=28.955223880597, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=28.955223880597, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=28.6245353159851, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157426893, Length=288, Percent_Identity=26.0416666666667, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=30.2816901408451, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=76.3959390862944, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=76.3959390862944, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=32.4483775811209, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=31.6666666666667, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=27.8787878787879, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.3809523809524, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=43.8461538461538, Blast_Score=330, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=31.1258278145695, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=31.1258278145695, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=29.0322580645161, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=353, Percent_Identity=28.8951841359773, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=430, Percent_Identity=26.2790697674419, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=430, Percent_Identity=26.2790697674419, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=276, Percent_Identity=30.7971014492754, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.0952380952381, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=300, Percent_Identity=28.6666666666667, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.0386266094421, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=67.9292929292929, Blast_Score=547, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=29.1196388261851, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=29.1196388261851, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=25.1101321585903, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=27.7286135693215, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=289, Percent_Identity=28.719723183391, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=60.2564102564103, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=60.2564102564103, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.7046632124352, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=468, Percent_Identity=29.7008547008547, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553816, Length=468, Percent_Identity=29.7008547008547, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24651721, Length=468, Percent_Identity=29.7008547008547, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17864154, Length=468, Percent_Identity=29.7008547008547, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.0623608017817, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.0623608017817, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137380, Length=336, Percent_Identity=30.952380952381, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45550900, Length=335, Percent_Identity=28.6567164179104, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=29.0322580645161, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.3658536585366, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582462, Length=160, Percent_Identity=32.5, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43208; Mature: 43077

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAKFERNKPHVNIGTIGHVDHGKTTLTAAITITLAKTGGATAKNYADIDAAPEEKARG
CCCCCCCCCCCEEEECEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKVDMVDDEELLELVEMEVRELLSSYNFPGDDIPIIKGSALAAVE
HHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEE
DRNPEIGQDRILELMKAVDEYIPTPERPLDKPFLMPVEDVFSISGRGTVVTGRVEQGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCC
ITPHTLFEAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVKLPEDKEMVLPGDNVK
CCCCEEEECEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVELINPIAMDKGLRFAIREGGRTVGAGVVSEIKK
EEEEECCCHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
GKAKFERNKPHVNIGTIGHVDHGKTTLTAAITITLAKTGGATAKNYADIDAAPEEKARG
CCCCCCCCCCEEEECEEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKVDMVDDEELLELVEMEVRELLSSYNFPGDDIPIIKGSALAAVE
HHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEE
DRNPEIGQDRILELMKAVDEYIPTPERPLDKPFLMPVEDVFSISGRGTVVTGRVEQGIVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHHHHH
VGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNIGALLRGVDREGVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCC
ITPHTLFEAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVKLPEDKEMVLPGDNVK
CCCCEEEECEEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MDVELINPIAMDKGLRFAIREGGRTVGAGVVSEIKK
EEEEECCCHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA