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The map label for this gene is amtB [C]

Identifier: 114799360

GI number: 114799360

Start: 3679369

End: 3680730

Strand: Reverse

Name: amtB [C]

Synonym: HNE_3542

Alternate gene names: 114799360

Gene position: 3680730-3679369 (Counterclockwise)

Preceding gene: 114797817

Following gene: 114797355

Centisome position: 99.34

GC content: 61.75

Gene sequence:

>1362_bases
ATGGGCCAATTGATTGGACGATGGATCCGCGGGGCGACACTCGCGCCGCTGGCACTGCTCACAGCAGGTATGGCATTTGC
GCAGGAAGACGATGTGGCGACACGTCTGGCAGCGCTGGAAGAAGCGGTCGGCGGAAGCGCCAGCGCCTATGTGGATAACA
GCTACCTGTTCCTCATCGGCGGCTTGATCGTCATGTTCATGGCGGCAGGTTTCTGCATGCTGGAAGCAGGCCTGGTGCGC
TCCAAGAACGCAGCAATGCAATCTATGAAGAACGTCACCCTGTTCTCCGTTGCCGGCCTGATGTTCTGGATCACGGGCTA
CAATCTTGCCTATCCGGGTGACGCGACCCTCTTCAACGGTCTGCTGCCGACCAATTTCGGTCCCTGGAGCGGCGGCGATC
TGTCCGTCGAGTCCGATGGCGGCTACGCGCCGGCCTCTGACTGGTTCTTCCAGATGGTGTTCGTTGCCACCGCAGCCTCG
ATTGTCTCGGGCACCGTGGCAGAACGCGTCAAAATCTGGCCGTTCCTTATCTTCACCGCAGTCCTCACCGCATTCATCTA
TCCGGTTGTGGCCAGCTGGGAGTGGGGCGGCGGCTATCTCGACAGCGTCTGGAGCTTCTCCGACTTTGCCGGCTCGACCC
TGGTTCACTCGACCGGCGGCTGGGCAGCCCTGACAGGTGCCATCATCATCGGCGCACGCAGCGGCAAATATTCCGGCAAG
ACGGTCAACCCGATGCCGGGCTCCAATATCCCGCTCGCGGCTCTTGGCACCTTCATCCTGTGGTTCGGCTGGTTCGGCTT
CAACGGCGCCTCGCAGCTTGCTGCCGGCACCGCAGCTGACATCAACTCGGTCTCGCAGATCTTCGCCAACACCAATATGG
CCGCAGTCGGCGGTGTCCTCGCAGCGATGGTCACCACGGCCGTCCTCTACAAGGGCAAGCTCGACGCAACGCTGATCTTC
AACGGTGCCATCGGTGGCCTCGTTTCGATCACCGCTGAACCGCTGGCCCCGTCCATGGGCATGTCGGTCCTCATCGGCGC
AGTCGGCGGTGTCCTGGTGGTTGTCTCGGTTCCGCTCCTCGACAAGTTCAAGATCGATGACGTCGTCGGCGCCATCCCGG
CTCACCTTATCTGCGGCATCTGGGGCACGATGATCGTCCCCCTCACCAACAGCGGCGCCTCCTATGCAGGCCAGGCTGTC
GGTGTGGCCCTGACCGGTGTGTTCGTGGTCATCATCTCCTCGATCATCTGGTTCGCCCTCAAATTCACGATCGGCGTCCG
CCCGAGCGAGGAAGACGAGGAAATGGGTCTCGACCGGGCAGAACTCGGCCTGGAAGCCTACCCGGAATTCTCGAACCGGT
AA

Upstream 100 bases:

>100_bases
CATGTGCGACTTTTCCGGGCAGGCGCAATCATATCGCCATGGGAGAGCGCTGGGGGAATACCGGGCTCTGCGTGACGACA
AGGCAAGTAAGGAGGGGCCA

Downstream 100 bases:

>100_bases
TTGGCTGCCATCAGCCGCTAATCAGAGTAGAAATCCACAGACCATGGGCCCCGGCGGAGCGATCCGCCGGGGCTTTTCAC
ATTCCACGCTGGCGCGAAAC

Product: ammonium transporter family protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MGQLIGRWIRGATLAPLALLTAGMAFAQEDDVATRLAALEEAVGGSASAYVDNSYLFLIGGLIVMFMAAGFCMLEAGLVR
SKNAAMQSMKNVTLFSVAGLMFWITGYNLAYPGDATLFNGLLPTNFGPWSGGDLSVESDGGYAPASDWFFQMVFVATAAS
IVSGTVAERVKIWPFLIFTAVLTAFIYPVVASWEWGGGYLDSVWSFSDFAGSTLVHSTGGWAALTGAIIIGARSGKYSGK
TVNPMPGSNIPLAALGTFILWFGWFGFNGASQLAAGTAADINSVSQIFANTNMAAVGGVLAAMVTTAVLYKGKLDATLIF
NGAIGGLVSITAEPLAPSMGMSVLIGAVGGVLVVVSVPLLDKFKIDDVVGAIPAHLICGIWGTMIVPLTNSGASYAGQAV
GVALTGVFVVIISSIIWFALKFTIGVRPSEEDEEMGLDRAELGLEAYPEFSNR

Sequences:

>Translated_453_residues
MGQLIGRWIRGATLAPLALLTAGMAFAQEDDVATRLAALEEAVGGSASAYVDNSYLFLIGGLIVMFMAAGFCMLEAGLVR
SKNAAMQSMKNVTLFSVAGLMFWITGYNLAYPGDATLFNGLLPTNFGPWSGGDLSVESDGGYAPASDWFFQMVFVATAAS
IVSGTVAERVKIWPFLIFTAVLTAFIYPVVASWEWGGGYLDSVWSFSDFAGSTLVHSTGGWAALTGAIIIGARSGKYSGK
TVNPMPGSNIPLAALGTFILWFGWFGFNGASQLAAGTAADINSVSQIFANTNMAAVGGVLAAMVTTAVLYKGKLDATLIF
NGAIGGLVSITAEPLAPSMGMSVLIGAVGGVLVVVSVPLLDKFKIDDVVGAIPAHLICGIWGTMIVPLTNSGASYAGQAV
GVALTGVFVVIISSIIWFALKFTIGVRPSEEDEEMGLDRAELGLEAYPEFSNR
>Mature_452_residues
GQLIGRWIRGATLAPLALLTAGMAFAQEDDVATRLAALEEAVGGSASAYVDNSYLFLIGGLIVMFMAAGFCMLEAGLVRS
KNAAMQSMKNVTLFSVAGLMFWITGYNLAYPGDATLFNGLLPTNFGPWSGGDLSVESDGGYAPASDWFFQMVFVATAASI
VSGTVAERVKIWPFLIFTAVLTAFIYPVVASWEWGGGYLDSVWSFSDFAGSTLVHSTGGWAALTGAIIIGARSGKYSGKT
VNPMPGSNIPLAALGTFILWFGWFGFNGASQLAAGTAADINSVSQIFANTNMAAVGGVLAAMVTTAVLYKGKLDATLIFN
GAIGGLVSITAEPLAPSMGMSVLIGAVGGVLVVVSVPLLDKFKIDDVVGAIPAHLICGIWGTMIVPLTNSGASYAGQAVG
VALTGVFVVIISSIIWFALKFTIGVRPSEEDEEMGLDRAELGLEAYPEFSNR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=28.9855072463768, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=444, Percent_Identity=32.4324324324324, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17550182, Length=440, Percent_Identity=30.4545454545455, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=435, Percent_Identity=28.0459770114943, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=26.3403263403263, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=26.9503546099291, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=423, Percent_Identity=27.8959810874704, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=28.537170263789, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=31.0843373493976, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47184; Mature: 47053

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQLIGRWIRGATLAPLALLTAGMAFAQEDDVATRLAALEEAVGGSASAYVDNSYLFLIG
CCHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
GLIVMFMAAGFCMLEAGLVRSKNAAMQSMKNVTLFSVAGLMFWITGYNLAYPGDATLFNG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCEECCCCHHHHCC
LLPTNFGPWSGGDLSVESDGGYAPASDWFFQMVFVATAASIVSGTVAERVKIWPFLIFTA
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLTAFIYPVVASWEWGGGYLDSVWSFSDFAGSTLVHSTGGWAALTGAIIIGARSGKYSGK
HHHHHHHHHHHCCCCCCCHHHHHHCHHHHCCCEEEECCCCHHHEECEEEEEECCCCCCCC
TVNPMPGSNIPLAALGTFILWFGWFGFNGASQLAAGTAADINSVSQIFANTNMAAVGGVL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
AAMVTTAVLYKGKLDATLIFNGAIGGLVSITAEPLAPSMGMSVLIGAVGGVLVVVSVPLL
HHHHHHHHHHCCCCCEEEEECCCCCCHHEEECCCCCCCCHHHHHHHHHHHHEEHHCCCCC
DKFKIDDVVGAIPAHLICGIWGTMIVPLTNSGASYAGQAVGVALTGVFVVIISSIIWFAL
CCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFTIGVRPSEEDEEMGLDRAELGLEAYPEFSNR
HHHCCCCCCCCHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GQLIGRWIRGATLAPLALLTAGMAFAQEDDVATRLAALEEAVGGSASAYVDNSYLFLIG
CHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
GLIVMFMAAGFCMLEAGLVRSKNAAMQSMKNVTLFSVAGLMFWITGYNLAYPGDATLFNG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCEECCCCHHHHCC
LLPTNFGPWSGGDLSVESDGGYAPASDWFFQMVFVATAASIVSGTVAERVKIWPFLIFTA
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLTAFIYPVVASWEWGGGYLDSVWSFSDFAGSTLVHSTGGWAALTGAIIIGARSGKYSGK
HHHHHHHHHHHCCCCCCCHHHHHHCHHHHCCCEEEECCCCHHHEECEEEEEECCCCCCCC
TVNPMPGSNIPLAALGTFILWFGWFGFNGASQLAAGTAADINSVSQIFANTNMAAVGGVL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
AAMVTTAVLYKGKLDATLIFNGAIGGLVSITAEPLAPSMGMSVLIGAVGGVLVVVSVPLL
HHHHHHHHHHCCCCCEEEEECCCCCCHHEEECCCCCCCCHHHHHHHHHHHHEEHHCCCCC
DKFKIDDVVGAIPAHLICGIWGTMIVPLTNSGASYAGQAVGVALTGVFVVIISSIIWFAL
CCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFTIGVRPSEEDEEMGLDRAELGLEAYPEFSNR
HHHCCCCCCCCHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]