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The map label for this gene is bcr [H]

Identifier: 114799340

GI number: 114799340

Start: 3293840

End: 3295039

Strand: Direct

Name: bcr [H]

Synonym: HNE_3113

Alternate gene names: 114799340

Gene position: 3293840-3295039 (Clockwise)

Preceding gene: 114798153

Following gene: 114797670

Centisome position: 88.9

GC content: 63.08

Gene sequence:

>1200_bases
ATGCGTAAAGTCGAATTCGTGGCCATCGTCGCCGGCCTGATGGCGCTCAATGCGATCGCCATCGACATCATGCTGCCCGC
GCTCAGCGCCATCGCGCGCGACACCGGCCTCACCGCCGAAGGCGGCGACCAGAACCGCCAGCAGCTGATCATCTTTGCCT
ATGTGCTCGGCTTCGGCGCCCCCCAGCTGATCTGGGGACCGATCACCGACCGGTTCGGCCGGCGCGCGCCGCTGTTCATC
GGCCTGATCGGCTATGTGCTGACAGCCCTCCTCTGCATCATGGCGCACAGCTTCTGGGCCTTGCTCGCCGCGCGCTTCCT
GCAAGGAGTGTTCTCTTCGGGCGCCCGCACAGTCGCCTCCGCCGTGGTGCGCGACCTGTTCGCCGGGCGCAGCATGGCGA
GCTTCATGTCGCTGGTGATGACCATCTTCATGATCGTGCCGATCATCGCCCCGACCATCGGCCAGATCATTCTCTATGTG
GCGCCCTGGGAAGGCATCTTTGTCGTGCTCGCCGTCTGGGGCGCGCTGATGCTGGGCTGGACCTGGTTCCGCCTGCCCGA
AAGCCTGCCGAAGGAGGCCCGCCGGTCTCTGGAGATCGGCAGCGTCATGACAGCGTATCTTCAGGTCATCCGCACGCGGG
TGAGCTTTGGTTACATGCTGGCCTCTGGCCTCGTCTTCGGCTCGCTGTTTGCGTTCATCGCCTCGTCCGAACAGGTGTTC
CGTGACGTGTTCGATCAGGGCGACACCTTCGCCTACTGGTTTGCCGGCATTGCCGCCGCGTTGGCCGTATCGAACTTTGC
CAACTCGCGCCTTGTCGAGAAAGTCGGTATGCGCCGTATCAGCCATATCGCGCTTCTCACCTTCATGCTGTCCTCTCTGG
TGCTCACCCTGGCTACGCTGGCCTGGGGCGAAAACCTGGCCGTATTCTTCCCGCTGTTTGCGCTGGGCTTTGCCTGTTTC
GGCATGATGGGGTCAAATTTCTCGGCGCTGGCCATGGAGCCACTGGGTAAGATCGCGGGCACGGCGTCCGCCGCGTATGG
TTTTGCCACCACCATGATCTCCAGCATCATCGGCATGATCATCGGCTCGCAGTTTAACGGCACGACCGTTCCGCTCACGC
TCGGCTTCGTGATCCTCGGCGCCGCTTCGCTGGTGGTGATCCTGGTGACGGAAAAGGGCCGCCTGTTCAGCCGCCATTGA

Upstream 100 bases:

>100_bases
GCTTTCATCCTGGCGCTCGCCGGAAACTGGAAAGACCGGACCCATGGACGGAAGTGAAGCGGGCAAGGCCGACGGCCTTC
ACGCGCCCCAGCCGACAGGC

Downstream 100 bases:

>100_bases
GGGCTGCGTCAGAGCGGCTCCCGATGGCGCGACAGCGGACGCACGACCTTCTCCAGATGCTCGACGATACGGGCGGCAAT
GTCCTTGCCGGTCGCCTTCT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRKVEFVAIVAGLMALNAIAIDIMLPALSAIARDTGLTAEGGDQNRQQLIIFAYVLGFGAPQLIWGPITDRFGRRAPLFI
GLIGYVLTALLCIMAHSFWALLAARFLQGVFSSGARTVASAVVRDLFAGRSMASFMSLVMTIFMIVPIIAPTIGQIILYV
APWEGIFVVLAVWGALMLGWTWFRLPESLPKEARRSLEIGSVMTAYLQVIRTRVSFGYMLASGLVFGSLFAFIASSEQVF
RDVFDQGDTFAYWFAGIAAALAVSNFANSRLVEKVGMRRISHIALLTFMLSSLVLTLATLAWGENLAVFFPLFALGFACF
GMMGSNFSALAMEPLGKIAGTASAAYGFATTMISSIIGMIIGSQFNGTTVPLTLGFVILGAASLVVILVTEKGRLFSRH

Sequences:

>Translated_399_residues
MRKVEFVAIVAGLMALNAIAIDIMLPALSAIARDTGLTAEGGDQNRQQLIIFAYVLGFGAPQLIWGPITDRFGRRAPLFI
GLIGYVLTALLCIMAHSFWALLAARFLQGVFSSGARTVASAVVRDLFAGRSMASFMSLVMTIFMIVPIIAPTIGQIILYV
APWEGIFVVLAVWGALMLGWTWFRLPESLPKEARRSLEIGSVMTAYLQVIRTRVSFGYMLASGLVFGSLFAFIASSEQVF
RDVFDQGDTFAYWFAGIAAALAVSNFANSRLVEKVGMRRISHIALLTFMLSSLVLTLATLAWGENLAVFFPLFALGFACF
GMMGSNFSALAMEPLGKIAGTASAAYGFATTMISSIIGMIIGSQFNGTTVPLTLGFVILGAASLVVILVTEKGRLFSRH
>Mature_399_residues
MRKVEFVAIVAGLMALNAIAIDIMLPALSAIARDTGLTAEGGDQNRQQLIIFAYVLGFGAPQLIWGPITDRFGRRAPLFI
GLIGYVLTALLCIMAHSFWALLAARFLQGVFSSGARTVASAVVRDLFAGRSMASFMSLVMTIFMIVPIIAPTIGQIILYV
APWEGIFVVLAVWGALMLGWTWFRLPESLPKEARRSLEIGSVMTAYLQVIRTRVSFGYMLASGLVFGSLFAFIASSEQVF
RDVFDQGDTFAYWFAGIAAALAVSNFANSRLVEKVGMRRISHIALLTFMLSSLVLTLATLAWGENLAVFFPLFALGFACF
GMMGSNFSALAMEPLGKIAGTASAAYGFATTMISSIIGMIIGSQFNGTTVPLTLGFVILGAASLVVILVTEKGRLFSRH

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=392, Percent_Identity=28.0612244897959, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994889, Length=240, Percent_Identity=28.3333333333333, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790146, Length=350, Percent_Identity=26.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787065, Length=316, Percent_Identity=25.6329113924051, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082312, Length=192, Percent_Identity=29.6875, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790794, Length=257, Percent_Identity=23.3463035019455, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6325414, Length=103, Percent_Identity=31.0679611650485, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42986; Mature: 42986

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVEFVAIVAGLMALNAIAIDIMLPALSAIARDTGLTAEGGDQNRQQLIIFAYVLGFGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
PQLIWGPITDRFGRRAPLFIGLIGYVLTALLCIMAHSFWALLAARFLQGVFSSGARTVAS
CHHEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVVRDLFAGRSMASFMSLVMTIFMIVPIIAPTIGQIILYVAPWEGIFVVLAVWGALMLGW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHH
TWFRLPESLPKEARRSLEIGSVMTAYLQVIRTRVSFGYMLASGLVFGSLFAFIASSEQVF
HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RDVFDQGDTFAYWFAGIAAALAVSNFANSRLVEKVGMRRISHIALLTFMLSSLVLTLATL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWGENLAVFFPLFALGFACFGMMGSNFSALAMEPLGKIAGTASAAYGFATTMISSIIGMI
HCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IGSQFNGTTVPLTLGFVILGAASLVVILVTEKGRLFSRH
HCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
>Mature Secondary Structure
MRKVEFVAIVAGLMALNAIAIDIMLPALSAIARDTGLTAEGGDQNRQQLIIFAYVLGFGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
PQLIWGPITDRFGRRAPLFIGLIGYVLTALLCIMAHSFWALLAARFLQGVFSSGARTVAS
CHHEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AVVRDLFAGRSMASFMSLVMTIFMIVPIIAPTIGQIILYVAPWEGIFVVLAVWGALMLGW
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHH
TWFRLPESLPKEARRSLEIGSVMTAYLQVIRTRVSFGYMLASGLVFGSLFAFIASSEQVF
HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RDVFDQGDTFAYWFAGIAAALAVSNFANSRLVEKVGMRRISHIALLTFMLSSLVLTLATL
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWGENLAVFFPLFALGFACFGMMGSNFSALAMEPLGKIAGTASAAYGFATTMISSIIGMI
HCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IGSQFNGTTVPLTLGFVILGAASLVVILVTEKGRLFSRH
HCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]