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The map label for this gene is gatA

Identifier: 114799311

GI number: 114799311

Start: 2393715

End: 2395187

Strand: Reverse

Name: gatA

Synonym: HNE_2293

Alternate gene names: 114799311

Gene position: 2395187-2393715 (Counterclockwise)

Preceding gene: 114798687

Following gene: 114800360

Centisome position: 64.65

GC content: 62.73

Gene sequence:

>1473_bases
ATGACCGACCTGACCGATCTGACCCTCGCCGCCGCTCTGGATGGGCTGAAATCAAAGCAGTTTTCCTCTGCCGAGATTAC
AGGGGCCTTTCTGCAGGCGATGGAGAAGTCGCGCGTGCTGAACGCGTATGTCGTCGAGACGCCGGAAAAGGCCATGGAAA
TGGCCAGGGCTTCGGACGCCCGCATCGCCAAGGGCGAGGGCGGACGGCTGGAAGGCGCGCCGCTGGGGATCAAGGATCTC
TACTGCACCAAGGGCGTGCGCACGACGGCCTGTTCCAATATCCTGGGCGAGTTCACCCCCACCTACGAATCGACCGTCAC
CCAGAACCTCTGGGACGAAGGCGCCGTCATGCTGGGCAAGCTCAACATGGACGAGTTCGCCATGGGCTCGTCCAACGAGA
CTTCGCGGTTTGGTCCGCCGATCAACCCCTGGCGCCGTAAGGGCGACAATGCGGGGCTGACACCGGGCGGGTCTTCGGGC
GGCTCTGCGGCTGCCGTATCTGGCAATCTCTGCCTTGCGGCGACGGCGTCCGACACCGGCGGCTCCATCCGCCAGCCTGC
CTCGTTCACCGGTACGGTCGGCATCAAGCCAACTTATGGCCGCGCCAGCCGGTATGGCATGGTGGCGTTTGCCTCCTCGC
TGGATCAGGCCGGGCCGATTGCAAAAACGGTAGAAGACAGCGCGCTGTTGCTGGAAATCATGTGCAGCCATGATCCCAAG
GATTCCACCTCGCTGAAGGTTGAGACGCCGGACTGGCGCAGCGATGTGCGCAAGGGCGTCAAAGGCATGAAGATCGGCAT
TCCGAAGGAATACCGCATCGAGGGTCTCTCCGAAGAGATTGAGGCGCTGTGGCAGCAGGGCATTGCCTGGCTGAAAGCCG
CCGGCGCCGAGATTGTCGAGATTTCCCTGCCGCACACTAAATATGCCCTTCCGGCGTATTACATCGTGGCCCCGGCCGAA
GCCTCGTCCAACCTCGCCCGCTATGACGGCATGCGCTATGGCGCGCGGGTGGAAGGCAACAACCTGACGCAGACCTATGA
AGCCTCCCGCGCCAGCGGGTTCGGCAAGGAAGTGCAGCGCCGCCTGATGATCGGCACCTATGTGCTCTCCTCGGGCTATT
ATGATGCCTATTACCTCCGGGCACAGAAGGTGCGTGCTAAGATCTTCCAGGATTTTGTGGGCGCATTCGAGCAGTGCGAC
GCGATCCTGACGCCGGCCTGTCCGTCAACGGCCTTCGCATTTGGCGAGAAGTCTGGCGACCCGCTTGAGATGTACCTTAT
GGACGTTTTCACGGTGACGGCAAACCTTGCCGGCCTTCCGGGCATCTCCGTTCCGGCCGGCCTTTCGGCGAGCGGCCTGC
CGCTTGGCCTGCAAGTCATCGGCAAGGCGCTGGACGAGAGCGCGTGCTTCCGGGTGGGCGGCGTGCTGGAAGATGCAGCC
AGCTTTGTGGCCAAGCCGGATCGCTGGTGGTAA

Upstream 100 bases:

>100_bases
TGCACCCGCCCCGCAACCGCAGCAAGCGGCCTCACGGACACTTGAACTTTCAGGCATTGGCGCCTAGCAGGCGTGGGTTC
TTTTCCATGGGATTTCCCCG

Downstream 100 bases:

>100_bases
TCTCCGGGCTATGGTCCGTTATTTTGTAATCTTTGCGGCGACGCTCGGCATCGGCCTCGTGATGATGGCCGGTATTCTCG
CGCAGAATGGCTACAGCCTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTDLTDLTLAAALDGLKSKQFSSAEITGAFLQAMEKSRVLNAYVVETPEKAMEMARASDARIAKGEGGRLEGAPLGIKDL
YCTKGVRTTACSNILGEFTPTYESTVTQNLWDEGAVMLGKLNMDEFAMGSSNETSRFGPPINPWRRKGDNAGLTPGGSSG
GSAAAVSGNLCLAATASDTGGSIRQPASFTGTVGIKPTYGRASRYGMVAFASSLDQAGPIAKTVEDSALLLEIMCSHDPK
DSTSLKVETPDWRSDVRKGVKGMKIGIPKEYRIEGLSEEIEALWQQGIAWLKAAGAEIVEISLPHTKYALPAYYIVAPAE
ASSNLARYDGMRYGARVEGNNLTQTYEASRASGFGKEVQRRLMIGTYVLSSGYYDAYYLRAQKVRAKIFQDFVGAFEQCD
AILTPACPSTAFAFGEKSGDPLEMYLMDVFTVTANLAGLPGISVPAGLSASGLPLGLQVIGKALDESACFRVGGVLEDAA
SFVAKPDRWW

Sequences:

>Translated_490_residues
MTDLTDLTLAAALDGLKSKQFSSAEITGAFLQAMEKSRVLNAYVVETPEKAMEMARASDARIAKGEGGRLEGAPLGIKDL
YCTKGVRTTACSNILGEFTPTYESTVTQNLWDEGAVMLGKLNMDEFAMGSSNETSRFGPPINPWRRKGDNAGLTPGGSSG
GSAAAVSGNLCLAATASDTGGSIRQPASFTGTVGIKPTYGRASRYGMVAFASSLDQAGPIAKTVEDSALLLEIMCSHDPK
DSTSLKVETPDWRSDVRKGVKGMKIGIPKEYRIEGLSEEIEALWQQGIAWLKAAGAEIVEISLPHTKYALPAYYIVAPAE
ASSNLARYDGMRYGARVEGNNLTQTYEASRASGFGKEVQRRLMIGTYVLSSGYYDAYYLRAQKVRAKIFQDFVGAFEQCD
AILTPACPSTAFAFGEKSGDPLEMYLMDVFTVTANLAGLPGISVPAGLSASGLPLGLQVIGKALDESACFRVGGVLEDAA
SFVAKPDRWW
>Mature_489_residues
TDLTDLTLAAALDGLKSKQFSSAEITGAFLQAMEKSRVLNAYVVETPEKAMEMARASDARIAKGEGGRLEGAPLGIKDLY
CTKGVRTTACSNILGEFTPTYESTVTQNLWDEGAVMLGKLNMDEFAMGSSNETSRFGPPINPWRRKGDNAGLTPGGSSGG
SAAAVSGNLCLAATASDTGGSIRQPASFTGTVGIKPTYGRASRYGMVAFASSLDQAGPIAKTVEDSALLLEIMCSHDPKD
STSLKVETPDWRSDVRKGVKGMKIGIPKEYRIEGLSEEIEALWQQGIAWLKAAGAEIVEISLPHTKYALPAYYIVAPAEA
SSNLARYDGMRYGARVEGNNLTQTYEASRASGFGKEVQRRLMIGTYVLSSGYYDAYYLRAQKVRAKIFQDFVGAFEQCDA
ILTPACPSTAFAFGEKSGDPLEMYLMDVFTVTANLAGLPGISVPAGLSASGLPLGLQVIGKALDESACFRVGGVLEDAAS
FVAKPDRWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=39.3877551020408, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI195972892, Length=483, Percent_Identity=23.1884057971014, Blast_Score=96, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=37.9157427937916, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=24.8508946322068, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=23.8853503184713, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=203, Percent_Identity=29.5566502463054, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=203, Percent_Identity=29.5566502463054, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71990152, Length=307, Percent_Identity=26.0586319218241, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=226, Percent_Identity=25.6637168141593, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=425, Percent_Identity=36.2352941176471, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=458, Percent_Identity=25.5458515283843, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648113, Length=478, Percent_Identity=38.0753138075314, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=25.5060728744939, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24652985, Length=518, Percent_Identity=23.9382239382239, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922090, Length=518, Percent_Identity=23.9382239382239, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24652981, Length=518, Percent_Identity=23.9382239382239, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24652983, Length=518, Percent_Identity=23.9382239382239, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI161078093, Length=499, Percent_Identity=23.8476953907816, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=28.8702928870293, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=28.8702928870293, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=28.8702928870293, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_HYPNA (Q0BZV5)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760988.1
- ProteinModelPortal:   Q0BZV5
- SMR:   Q0BZV5
- STRING:   Q0BZV5
- GeneID:   4287320
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2293
- NMPDR:   fig|228405.5.peg.2210
- TIGR:   HNE_2293
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q0BZV5
- ProtClustDB:   PRK00012
- BioCyc:   HNEP81032:HNE_2293-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52227; Mature: 52096

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTDLTLAAALDGLKSKQFSSAEITGAFLQAMEKSRVLNAYVVETPEKAMEMARASDA
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCC
RIAKGEGGRLEGAPLGIKDLYCTKGVRTTACSNILGEFTPTYESTVTQNLWDEGAVMLGK
EEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
LNMDEFAMGSSNETSRFGPPINPWRRKGDNAGLTPGGSSGGSAAAVSGNLCLAATASDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCC
GSIRQPASFTGTVGIKPTYGRASRYGMVAFASSLDQAGPIAKTVEDSALLLEIMCSHDPK
CCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
DSTSLKVETPDWRSDVRKGVKGMKIGIPKEYRIEGLSEEIEALWQQGIAWLKAAGAEIVE
CCCEEEEECCCHHHHHHHCCCCCEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYIVAPAEASSNLARYDGMRYGARVEGNNLTQTYEASRASGFGKEVQR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCEECCCCCCHHHHHHHHCCCHHHHHH
RLMIGTYVLSSGYYDAYYLRAQKVRAKIFQDFVGAFEQCDAILTPACPSTAFAFGEKSGD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCC
PLEMYLMDVFTVTANLAGLPGISVPAGLSASGLPLGLQVIGKALDESACFRVGGVLEDAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
SFVAKPDRWW
HHHCCCCCCC
>Mature Secondary Structure 
TDLTDLTLAAALDGLKSKQFSSAEITGAFLQAMEKSRVLNAYVVETPEKAMEMARASDA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCC
RIAKGEGGRLEGAPLGIKDLYCTKGVRTTACSNILGEFTPTYESTVTQNLWDEGAVMLGK
EEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEE
LNMDEFAMGSSNETSRFGPPINPWRRKGDNAGLTPGGSSGGSAAAVSGNLCLAATASDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCCC
GSIRQPASFTGTVGIKPTYGRASRYGMVAFASSLDQAGPIAKTVEDSALLLEIMCSHDPK
CCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
DSTSLKVETPDWRSDVRKGVKGMKIGIPKEYRIEGLSEEIEALWQQGIAWLKAAGAEIVE
CCCEEEEECCCHHHHHHHCCCCCEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYIVAPAEASSNLARYDGMRYGARVEGNNLTQTYEASRASGFGKEVQR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCEECCCCCCHHHHHHHHCCCHHHHHH
RLMIGTYVLSSGYYDAYYLRAQKVRAKIFQDFVGAFEQCDAILTPACPSTAFAFGEKSGD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHCCCCCCC
PLEMYLMDVFTVTANLAGLPGISVPAGLSASGLPLGLQVIGKALDESACFRVGGVLEDAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
SFVAKPDRWW
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA