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The map label for this gene is alkB [H]

Identifier: 114799223

GI number: 114799223

Start: 1556123

End: 1556773

Strand: Reverse

Name: alkB [H]

Synonym: HNE_1522

Alternate gene names: 114799223

Gene position: 1556773-1556123 (Counterclockwise)

Preceding gene: 114799953

Following gene: 114798312

Centisome position: 42.02

GC content: 63.13

Gene sequence:

>651_bases
ATGACGGACCTGTTCGGCGCAGAGGAACCTCGCCAGACTGCACGGGAAAAACTGGCTGAAGGTGCTGTCCTGCTACGCGG
CTTCGCGCTGGATTGCGAGGTGGATCTGCTCGCCGCGATCCAGGCAATCACCACAGTGTCACCCTTTCGCAGAATGGCCA
CGCCCGGGGGTCACGTGATGTCCGTCGCGATGACGAACTGCGGTCAGGCCGGTTGGGTGACCGATCGCACCGGCTATCAC
TATGATGCCACGGACCCCGAAACCGGAAAGCCCTGGCACCCGATGCCCGAGAGCTTTATGGCACTGGCGGTCTTCGCCGC
GACCGAGGCAGGATATTGCAGGTTCCGCCCCGATACATGCCTCATCAACCGCTATGAACCTGGCGCGAAGCTGTCGCTTC
ATCAGGACAGGAACGAACGCGAGTTCGCCCATCCGATTGTTTCCGTTTCGCTCGGCCTGCCCGCGACTTTCCAGTTCGGT
GGCCTGAGGCGCGCAGACCCGATCAGGAAGTATGCGCTGCGCCATGGCGATGTCGCCGTGTGGGGCGGCCCGTCGCGCCT
CTGCCATCACGGCGTCCTGGCCCTGAAGGAGGGCGCGCATCCGAAACTGGGGCGGATGCGTCTAAATCTGACCTTTCGCG
GTGCACTCTAG

Upstream 100 bases:

>100_bases
CCAGGGCTCAGGCCAGTGAATACGTATTTCCTGCACTCCGGCCCGCTTCTGGCGTGATACAGACCATTCCTGTATCCTTC
TGCTCAGGGAGACACAGTTT

Downstream 100 bases:

>100_bases
GGCGTCAAAATTGAATATAGTTTTCAATGTTTTAAACCCCCTAAATCAGTCCCTCAATCAATTTGTATCCCATTTTGAAC
ATTGATCAGGATACACCCGG

Product: alkylated DNA repair protein AlkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTDLFGAEEPRQTAREKLAEGAVLLRGFALDCEVDLLAAIQAITTVSPFRRMATPGGHVMSVAMTNCGQAGWVTDRTGYH
YDATDPETGKPWHPMPESFMALAVFAATEAGYCRFRPDTCLINRYEPGAKLSLHQDRNEREFAHPIVSVSLGLPATFQFG
GLRRADPIRKYALRHGDVAVWGGPSRLCHHGVLALKEGAHPKLGRMRLNLTFRGAL

Sequences:

>Translated_216_residues
MTDLFGAEEPRQTAREKLAEGAVLLRGFALDCEVDLLAAIQAITTVSPFRRMATPGGHVMSVAMTNCGQAGWVTDRTGYH
YDATDPETGKPWHPMPESFMALAVFAATEAGYCRFRPDTCLINRYEPGAKLSLHQDRNEREFAHPIVSVSLGLPATFQFG
GLRRADPIRKYALRHGDVAVWGGPSRLCHHGVLALKEGAHPKLGRMRLNLTFRGAL
>Mature_215_residues
TDLFGAEEPRQTAREKLAEGAVLLRGFALDCEVDLLAAIQAITTVSPFRRMATPGGHVMSVAMTNCGQAGWVTDRTGYHY
DATDPETGKPWHPMPESFMALAVFAATEAGYCRFRPDTCLINRYEPGAKLSLHQDRNEREFAHPIVSVSLGLPATFQFGG
LRRADPIRKYALRHGDVAVWGGPSRLCHHGVLALKEGAHPKLGRMRLNLTFRGAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=122, Percent_Identity=31.9672131147541, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788541, Length=215, Percent_Identity=59.0697674418605, Blast_Score=253, Evalue=6e-69,
Organism=Drosophila melanogaster, GI45555401, Length=122, Percent_Identity=34.4262295081967, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23743; Mature: 23611

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLFGAEEPRQTAREKLAEGAVLLRGFALDCEVDLLAAIQAITTVSPFRRMATPGGHVM
CCCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
SVAMTNCGQAGWVTDRTGYHYDATDPETGKPWHPMPESFMALAVFAATEAGYCRFRPDTC
EEEECCCCCCCCEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEE
LINRYEPGAKLSLHQDRNEREFAHPIVSVSLGLPATFQFGGLRRADPIRKYALRHGDVAV
EEECCCCCCEEEECCCCCCHHHHCCEEEEECCCCCEEECCCCCCCCHHHHHHHHCCCEEE
WGGPSRLCHHGVLALKEGAHPKLGRMRLNLTFRGAL
ECCHHHHHHHCCEEECCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
TDLFGAEEPRQTAREKLAEGAVLLRGFALDCEVDLLAAIQAITTVSPFRRMATPGGHVM
CCCCCCCCHHHHHHHHHHCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
SVAMTNCGQAGWVTDRTGYHYDATDPETGKPWHPMPESFMALAVFAATEAGYCRFRPDTC
EEEECCCCCCCCEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEE
LINRYEPGAKLSLHQDRNEREFAHPIVSVSLGLPATFQFGGLRRADPIRKYALRHGDVAV
EEECCCCCCEEEECCCCCCHHHHCCEEEEECCCCCEEECCCCCCCCHHHHHHHHCCCEEE
WGGPSRLCHHGVLALKEGAHPKLGRMRLNLTFRGAL
ECCHHHHHHHCCEEECCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]