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The map label for this gene is citE [H]

Identifier: 114799185

GI number: 114799185

Start: 3666591

End: 3667469

Strand: Reverse

Name: citE [H]

Synonym: HNE_3534

Alternate gene names: 114799185

Gene position: 3667469-3666591 (Counterclockwise)

Preceding gene: 114798108

Following gene: 114797794

Centisome position: 98.99

GC content: 63.48

Gene sequence:

>879_bases
ATGTCCACCCAGCACCGTCCCCGCCGCTCCTGCCTCTACATGCCCGGCGCCAACACGCGCGCCCTGGAAAAGGCCCGCGA
TCTGCCCGCCGATACGCTGATCCTGGATCTGGAAGACGCCGTTGCCCCGGACGCCAAGGAAGAAGCCCGCGCGGCAATCC
TCGCCGCGGTGAAGGCCGGCGGCTATGGCAAGCGCGAAATCGTCATCCGCATGAACGGTCTCGACACGCCCTGGGGCCAT
GACGATCTCAAAGCCATCGCCACCTCCGGCGCTCATGCCGTCCTTGCGCCCAAGGTCACCGGCCACCGCGACATCGAGCG
CCTCAGCACCCTGCTGAAGGAAGCGGGCGCCTCCGATACGATGGCCCTCTGGGTGATGATTGAAACGCCTCTGGCAATTC
TCAACATCAAGGACATCGCTGCCGCTTCCATCGGAACGCATCTCACCACCTTCGTTATGGGCACGAATGATCTGGCCAAG
GAATACCGTGCCCGGATGACCCCCGACCGGCTCGCCTTCCAGACCGCGCTCCAGCTCTCGATCGCAGCGGCCCGCGCCTA
CGGCCTCGTTGCGATTGATGGCGTCTACAATGACATCAAGAACGAAAACGGCTTCCGCGATGAGTGCGAGCAGGGCCGCG
ATCTCGGCTTTGACGGCAAAACGCTGATCCACCCGTCCCAGCTGGAAGAGGCCAACCGCATTTTCGCGCCCAGCCATCAT
GATGTGGAACAGGCCAAAGCCGTGATCGAAGCCTTCGCCGACCCCGCCAATGCGGGGAAGGGTGTGCTGAAGGTCAACGG
CAAGATGACTGAGCTGCTCCACCTAGAGGAAGCGCGGCGGACGGTAGAGATGGATGAGGCGATCCGGGCGCTTGCCTGA

Upstream 100 bases:

>100_bases
GCGAAATCGGCCGGGTTTGCCCGTCTGATCTGGACATCTCCGCCCATGCGTTGCATCGCTCCCCTCTGAAACCGCCTCAG
ATGTAAGGGTCACCCCAGCC

Downstream 100 bases:

>100_bases
CGCCTCTCGACATGCTGCCGGGTCATCCTAAGTCAGTCTCAGGAAACCTCAGGAGGCCCGACCATGACCTATATTCTCAT
CCACAATCCCGGCTGCGGCT

Product: citrate lyase subunit beta

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSTQHRPRRSCLYMPGANTRALEKARDLPADTLILDLEDAVAPDAKEEARAAILAAVKAGGYGKREIVIRMNGLDTPWGH
DDLKAIATSGAHAVLAPKVTGHRDIERLSTLLKEAGASDTMALWVMIETPLAILNIKDIAAASIGTHLTTFVMGTNDLAK
EYRARMTPDRLAFQTALQLSIAAARAYGLVAIDGVYNDIKNENGFRDECEQGRDLGFDGKTLIHPSQLEEANRIFAPSHH
DVEQAKAVIEAFADPANAGKGVLKVNGKMTELLHLEEARRTVEMDEAIRALA

Sequences:

>Translated_292_residues
MSTQHRPRRSCLYMPGANTRALEKARDLPADTLILDLEDAVAPDAKEEARAAILAAVKAGGYGKREIVIRMNGLDTPWGH
DDLKAIATSGAHAVLAPKVTGHRDIERLSTLLKEAGASDTMALWVMIETPLAILNIKDIAAASIGTHLTTFVMGTNDLAK
EYRARMTPDRLAFQTALQLSIAAARAYGLVAIDGVYNDIKNENGFRDECEQGRDLGFDGKTLIHPSQLEEANRIFAPSHH
DVEQAKAVIEAFADPANAGKGVLKVNGKMTELLHLEEARRTVEMDEAIRALA
>Mature_291_residues
STQHRPRRSCLYMPGANTRALEKARDLPADTLILDLEDAVAPDAKEEARAAILAAVKAGGYGKREIVIRMNGLDTPWGHD
DLKAIATSGAHAVLAPKVTGHRDIERLSTLLKEAGASDTMALWVMIETPLAILNIKDIAAASIGTHLTTFVMGTNDLAKE
YRARMTPDRLAFQTALQLSIAAARAYGLVAIDGVYNDIKNENGFRDECEQGRDLGFDGKTLIHPSQLEEANRIFAPSHHD
VEQAKAVIEAFADPANAGKGVLKVNGKMTELLHLEEARRTVEMDEAIRALA

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=302, Percent_Identity=29.8013245033113, Blast_Score=141, Evalue=6e-34,
Organism=Escherichia coli, GI87081764, Length=279, Percent_Identity=35.84229390681, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17557458, Length=303, Percent_Identity=28.0528052805281, Blast_Score=120, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31698; Mature: 31566

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQHRPRRSCLYMPGANTRALEKARDLPADTLILDLEDAVAPDAKEEARAAILAAVKAG
CCCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHCC
GYGKREIVIRMNGLDTPWGHDDLKAIATSGAHAVLAPKVTGHRDIERLSTLLKEAGASDT
CCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
MALWVMIETPLAILNIKDIAAASIGTHLTTFVMGTNDLAKEYRARMTPDRLAFQTALQLS
EEEEEEEECCEEEEEHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCHHHHHHHHHHHHH
IAAARAYGLVAIDGVYNDIKNENGFRDECEQGRDLGFDGKTLIHPSQLEEANRIFAPSHH
HHHHHHHCEEEECCHHHHHCCCCCCHHHHHCCCCCCCCCCEEECHHHHHHHHCCCCCCCC
DVEQAKAVIEAFADPANAGKGVLKVNGKMTELLHLEEARRTVEMDEAIRALA
CHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STQHRPRRSCLYMPGANTRALEKARDLPADTLILDLEDAVAPDAKEEARAAILAAVKAG
CCCCCCCCCEEECCCCCHHHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHHHHHHCC
GYGKREIVIRMNGLDTPWGHDDLKAIATSGAHAVLAPKVTGHRDIERLSTLLKEAGASDT
CCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCC
MALWVMIETPLAILNIKDIAAASIGTHLTTFVMGTNDLAKEYRARMTPDRLAFQTALQLS
EEEEEEEECCEEEEEHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCHHHHHHHHHHHHH
IAAARAYGLVAIDGVYNDIKNENGFRDECEQGRDLGFDGKTLIHPSQLEEANRIFAPSHH
HHHHHHHCEEEECCHHHHHCCCCCCHHHHHCCCCCCCCCCEEECHHHHHHHHCCCCCCCC
DVEQAKAVIEAFADPANAGKGVLKVNGKMTELLHLEEARRTVEMDEAIRALA
CHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA