Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 114799161

GI number: 114799161

Start: 732490

End: 733872

Strand: Reverse

Name: radA [H]

Synonym: HNE_0720

Alternate gene names: 114799161

Gene position: 733872-732490 (Counterclockwise)

Preceding gene: 114798614

Following gene: 114798180

Centisome position: 19.81

GC content: 67.03

Gene sequence:

>1383_bases
ATGGCGAAAACGCCCACGTCCGCTTTTGTCTGCCAGTCCTGCGGCGCGGTTGCGGCCAAATGGGCCGGGCGCTGCGAGGC
GTGCGGGGAATGGAATACGCTGGTTGAAGAGGCCGTCTCTGCCCCCCCCGGCGCGTTGAAGGCTGAGAAAACGGGGACCA
AGCGCGGGCCGAAGGCGGAGTTTGTCGCGCTGGACGCGGAGACAGAGACGCCGGACCGGATCGTCATCGGCGTGGAAGAA
CTCGACCGGGTGTTCGGCGGCGGCATCGTGCCTGCCTCTGCCACGCTGATCGGGGGCGATCCGGGTATCGGAAAGTCCAC
GCTTCTTTTGCAGGCGGCCGCGCGTCTCGCCCGCAATGGCGTGAAGACGGTTTATGTGTCGGGCGAGGAAGCCGCTGCGC
AGATCCAGGAACGGGCCAAGCGGCTGAAGGTGGCCAATTCGCCGGTCCAGCTCGCCACCGAGACAGACCTGCGCAAGATC
CTCGCCGCGCTGAAGGAAGGCGCGCCCGATTTTGTGGTGATCGATTCCATTCAGACGCTCTGGTCGGACAGCCTGGAGGC
TGCGCCGGGCTCCGTCGCGCAGGTGCGGGCGTGCGCGCAGGAGCTGGTGCGCTGGGCGAAGAAGTCCGGCGCCGCGCTGG
TGCTGGTCGGCCACGTCACCAAGGAGGGCACCATCGCCGGGCCGCGCGTGGTGGAGCATATGGTCGACGCTGTGTTCTAT
TTTGAAGGCGAGCGCGGGCACCAGTTCCGCATCTTGCGGGCCGTGAAGAACCGCTTCGGACCCACTGACGAAATTGGCAT
TTTCGAGATGCACACCTACGGCCTCGCCCCGGCCAAGGAGCCTTCGGCGCTGTTCCTCTCCACCGAGGATGAAGGCGGGG
GCGGCACGGCGGTGTTTGCCGCCATGGAAGGCTCGCGCCCGGTGCTGGCCGAGGTGCAGGCGCTGGTGGCCAAGAGCCCG
TTCGGCACGCCCCGGCGCAGCGTGATCGGCTTTGATGCCGGGCGGCTCGCCATGCTGATGGCGGTTCTGGAGACCCGCTG
CGGGGTGAACTTTGCAGGTATGGATGTCTATCTGTCGGTCGCGGGGGGGTACAAGATTACCGAGCCGGCAGGCGATCTGG
CGGCGGCGGCAGCCCTCCTCTCATCGCTGGCCGGAAACCCGGTGCCCAAGCGCTCTGTGTTCTTCGGCGAGATCGCGCTG
TCTGGCGCCGTCAGGCCAGCCCCGCGTATGGACCAGCGCCTGAAGGAGGCGGCAAGGCTCGGTTTCCACACGGCATTCGT
TCCCGAGGGCAGCCCAGCAGCAGAGAGCGGCTTGACGGTGACCCCGATTGCGCGGCTTATCGGCCTCGTCGATTTACTCG
CGCCGGACGCGCCTGATGCTTGA

Upstream 100 bases:

>100_bases
CGGTCTGGAAACCACCGGTTCAGGTGGGGTTGCCGTTTGCGCTCGCCCGGCCCCGCGCCTAGACTCCTGACCTTGCAATT
CCAATGGAAACCGATTCCCC

Downstream 100 bases:

>100_bases
AAACATTTCAGCTTTTGACGGAATCGTCGTGGGCGTGATCATCGTTTCGGCGATCATGGCCTTTGCGCGCGGGTTCCTGC
GAGAACTCGCCACGCTGGGC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKTPTSAFVCQSCGAVAAKWAGRCEACGEWNTLVEEAVSAPPGALKAEKTGTKRGPKAEFVALDAETETPDRIVIGVEE
LDRVFGGGIVPASATLIGGDPGIGKSTLLLQAAARLARNGVKTVYVSGEEAAAQIQERAKRLKVANSPVQLATETDLRKI
LAALKEGAPDFVVIDSIQTLWSDSLEAAPGSVAQVRACAQELVRWAKKSGAALVLVGHVTKEGTIAGPRVVEHMVDAVFY
FEGERGHQFRILRAVKNRFGPTDEIGIFEMHTYGLAPAKEPSALFLSTEDEGGGGTAVFAAMEGSRPVLAEVQALVAKSP
FGTPRRSVIGFDAGRLAMLMAVLETRCGVNFAGMDVYLSVAGGYKITEPAGDLAAAAALLSSLAGNPVPKRSVFFGEIAL
SGAVRPAPRMDQRLKEAARLGFHTAFVPEGSPAAESGLTVTPIARLIGLVDLLAPDAPDA

Sequences:

>Translated_460_residues
MAKTPTSAFVCQSCGAVAAKWAGRCEACGEWNTLVEEAVSAPPGALKAEKTGTKRGPKAEFVALDAETETPDRIVIGVEE
LDRVFGGGIVPASATLIGGDPGIGKSTLLLQAAARLARNGVKTVYVSGEEAAAQIQERAKRLKVANSPVQLATETDLRKI
LAALKEGAPDFVVIDSIQTLWSDSLEAAPGSVAQVRACAQELVRWAKKSGAALVLVGHVTKEGTIAGPRVVEHMVDAVFY
FEGERGHQFRILRAVKNRFGPTDEIGIFEMHTYGLAPAKEPSALFLSTEDEGGGGTAVFAAMEGSRPVLAEVQALVAKSP
FGTPRRSVIGFDAGRLAMLMAVLETRCGVNFAGMDVYLSVAGGYKITEPAGDLAAAAALLSSLAGNPVPKRSVFFGEIAL
SGAVRPAPRMDQRLKEAARLGFHTAFVPEGSPAAESGLTVTPIARLIGLVDLLAPDAPDA
>Mature_459_residues
AKTPTSAFVCQSCGAVAAKWAGRCEACGEWNTLVEEAVSAPPGALKAEKTGTKRGPKAEFVALDAETETPDRIVIGVEEL
DRVFGGGIVPASATLIGGDPGIGKSTLLLQAAARLARNGVKTVYVSGEEAAAQIQERAKRLKVANSPVQLATETDLRKIL
AALKEGAPDFVVIDSIQTLWSDSLEAAPGSVAQVRACAQELVRWAKKSGAALVLVGHVTKEGTIAGPRVVEHMVDAVFYF
EGERGHQFRILRAVKNRFGPTDEIGIFEMHTYGLAPAKEPSALFLSTEDEGGGGTAVFAAMEGSRPVLAEVQALVAKSPF
GTPRRSVIGFDAGRLAMLMAVLETRCGVNFAGMDVYLSVAGGYKITEPAGDLAAAAALLSSLAGNPVPKRSVFFGEIALS
GAVRPAPRMDQRLKEAARLGFHTAFVPEGSPAAESGLTVTPIARLIGLVDLLAPDAPDA

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=432, Percent_Identity=44.9074074074074, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47997; Mature: 47865

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTPTSAFVCQSCGAVAAKWAGRCEACGEWNTLVEEAVSAPPGALKAEKTGTKRGPKAE
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
FVALDAETETPDRIVIGVEELDRVFGGGIVPASATLIGGDPGIGKSTLLLQAAARLARNG
EEEEECCCCCCCEEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
VKTVYVSGEEAAAQIQERAKRLKVANSPVQLATETDLRKILAALKEGAPDFVVIDSIQTL
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCEEEECCHHHH
WSDSLEAAPGSVAQVRACAQELVRWAKKSGAALVLVGHVTKEGTIAGPRVVEHMVDAVFY
HCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEE
FEGERGHQFRILRAVKNRFGPTDEIGIFEMHTYGLAPAKEPSALFLSTEDEGGGGTAVFA
EECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEE
AMEGSRPVLAEVQALVAKSPFGTPRRSVIGFDAGRLAMLMAVLETRCGVNFAGMDVYLSV
EECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEECCEEEEEE
AGGYKITEPAGDLAAAAALLSSLAGNPVPKRSVFFGEIALSGAVRPAPRMDQRLKEAARL
ECCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHH
GFHTAFVPEGSPAAESGLTVTPIARLIGLVDLLAPDAPDA
CCCEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKTPTSAFVCQSCGAVAAKWAGRCEACGEWNTLVEEAVSAPPGALKAEKTGTKRGPKAE
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCE
FVALDAETETPDRIVIGVEELDRVFGGGIVPASATLIGGDPGIGKSTLLLQAAARLARNG
EEEEECCCCCCCEEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
VKTVYVSGEEAAAQIQERAKRLKVANSPVQLATETDLRKILAALKEGAPDFVVIDSIQTL
CEEEEECCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHCCCCCEEEECCHHHH
WSDSLEAAPGSVAQVRACAQELVRWAKKSGAALVLVGHVTKEGTIAGPRVVEHMVDAVFY
HCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHEE
FEGERGHQFRILRAVKNRFGPTDEIGIFEMHTYGLAPAKEPSALFLSTEDEGGGGTAVFA
EECCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEEE
AMEGSRPVLAEVQALVAKSPFGTPRRSVIGFDAGRLAMLMAVLETRCGVNFAGMDVYLSV
EECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEECCEEEEEE
AGGYKITEPAGDLAAAAALLSSLAGNPVPKRSVFFGEIALSGAVRPAPRMDQRLKEAARL
ECCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHH
GFHTAFVPEGSPAAESGLTVTPIARLIGLVDLLAPDAPDA
CCCEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]