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The map label for this gene is greA

Identifier: 114799104

GI number: 114799104

Start: 646081

End: 646554

Strand: Reverse

Name: greA

Synonym: HNE_0643

Alternate gene names: 114799104

Gene position: 646554-646081 (Counterclockwise)

Preceding gene: 114798818

Following gene: 114799325

Centisome position: 17.45

GC content: 59.49

Gene sequence:

>474_bases
ATGGAACGCATTCCGATGACCGCCGAAGGGCATGCCGCGCTTCAGGCTGAACTGAAGGTGCTCAAATCCGTTGAGCGCCC
AAGCATCATTGCGGCAATTTCCGAAGCCCGCTCGCATGGCGACCTGTCGGAGAACGCCGAATATCACGCGGCCAAGGAGA
AGCAGAGCTTCATCGAAGGCCGTATCAGCGAGCTGGACGACAAGCTGGCGCGCGCTGACGTGATCGATGTGTCCAAGCTG
GGCGGCTCGAAAGTGCGCTTTGGTGCAACTGTAACCATTGCGGATGTCGACACCGAAGACGAGCAGACCTACAAGATCGT
GGGCGAGGACGAAGCGGATGTGAAACAGGGCAAGATCTCTGTCACCTCCCCTATCGCCCGCGCACTGATCGGCAAGGAAG
AAGGCGACGAGGCCGAAGTAGCCGCGCCCGCCGGCGCCCGCGCCTATGAGGTTATCAAGGTCGTTTACAAGTAA

Upstream 100 bases:

>100_bases
AATCTCCGTGCGCGCCTTGCAAAGCGCCTGATCGGCCACCACTTGACGCCCTTGGTTTTTGCGACAACGCTTTTTGCCGC
TGTGAGTAAGGGAATTTTCG

Downstream 100 bases:

>100_bases
GGATCAAAGGCATGGGGTCGCTCGGACCGTCTGTCTGGGCGGTTTCGGATGGCCGCGCCGGAAATGCCGCGCAGGTGCGT
GCGCTGACGTCCGCTTTGGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MERIPMTAEGHAALQAELKVLKSVERPSIIAAISEARSHGDLSENAEYHAAKEKQSFIEGRISELDDKLARADVIDVSKL
GGSKVRFGATVTIADVDTEDEQTYKIVGEDEADVKQGKISVTSPIARALIGKEEGDEAEVAAPAGARAYEVIKVVYK

Sequences:

>Translated_157_residues
MERIPMTAEGHAALQAELKVLKSVERPSIIAAISEARSHGDLSENAEYHAAKEKQSFIEGRISELDDKLARADVIDVSKL
GGSKVRFGATVTIADVDTEDEQTYKIVGEDEADVKQGKISVTSPIARALIGKEEGDEAEVAAPAGARAYEVIKVVYK
>Mature_157_residues
MERIPMTAEGHAALQAELKVLKSVERPSIIAAISEARSHGDLSENAEYHAAKEKQSFIEGRISELDDKLARADVIDVSKL
GGSKVRFGATVTIADVDTEDEQTYKIVGEDEADVKQGKISVTSPIARALIGKEEGDEAEVAAPAGARAYEVIKVVYK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=59.8726114649682, Blast_Score=186, Evalue=6e-49,
Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=38.4105960264901, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_HYPNA (Q0C4H1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759372.1
- ProteinModelPortal:   Q0C4H1
- SMR:   Q0C4H1
- STRING:   Q0C4H1
- GeneID:   4289203
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0643
- NMPDR:   fig|228405.5.peg.631
- TIGR:   HNE_0643
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q0C4H1
- BioCyc:   HNEP81032:HNE_0643-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 16880; Mature: 16880

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIPMTAEGHAALQAELKVLKSVERPSIIAAISEARSHGDLSENAEYHAAKEKQSFIEG
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RISELDDKLARADVIDVSKLGGSKVRFGATVTIADVDTEDEQTYKIVGEDEADVKQGKIS
HHHHHHHHHHHHCHHHHHHCCCCEEEECEEEEEEECCCCCCCEEEEECCCCCCHHCCEEE
VTSPIARALIGKEEGDEAEVAAPAGARAYEVIKVVYK
EHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MERIPMTAEGHAALQAELKVLKSVERPSIIAAISEARSHGDLSENAEYHAAKEKQSFIEG
CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RISELDDKLARADVIDVSKLGGSKVRFGATVTIADVDTEDEQTYKIVGEDEADVKQGKIS
HHHHHHHHHHHHCHHHHHHCCCCEEEECEEEEEEECCCCCCCEEEEECCCCCCHHCCEEE
VTSPIARALIGKEEGDEAEVAAPAGARAYEVIKVVYK
EHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA