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The map label for this gene is gspE [C]

Identifier: 114799052

GI number: 114799052

Start: 1164266

End: 1165654

Strand: Direct

Name: gspE [C]

Synonym: HNE_1153

Alternate gene names: 114799052

Gene position: 1164266-1165654 (Clockwise)

Preceding gene: 114799365

Following gene: 114797844

Centisome position: 31.42

GC content: 61.7

Gene sequence:

>1389_bases
ATGAGCCGGTTTGGATTTACCAATGCGGCGCCGAAGGTGGCCGAGGCTGTTCCGGCGCCGGTCGCTCCGCAAGCAGCGCC
TGCGGTGTCTCCCAAACAGACGCAGGCCGAGCAGGCATCGTTTGACCTTCTGGACGCAAAGCTCCGCGTGCATGCAAAGC
TGATTGACGAGCTGGACCTCTCCGCGCTGGACAAGCTGGACGATGAGACCATGCGGCGGCGCGTGCGCGGCATCATCAGC
GAGATTATCCGCAAGGAGGAGATGGCGCTCTCCGCTGCCGAGGAAGCGTCATTTGCCGACGCCGTGATGGACGAGATGAC
CGGGCTGGGGCCGATTGAGCCGCTGCTGAAAGACGATTCCATCGCGGATATTCTGATCAATGGCTGCAACCAGGTTTACA
TTGAGCGGGGCGGAAAGCTGCAACTGGCGCCGGTGCGCTTTGCGGATAATGATCACCTGTTGCGGATCGTGCAGCGGATT
GTGGCGGCGGTCGGGCGGCGCGTGGATGAGAGCCAGCCGCTGGTGGACGCGCGTCTGCTGGACGGCAGCCGGGTGAACGC
CGCAGTCGCGCCCATCGCCATTGACGGGCCACTCGTCTCTATCCGGAAGTTTTCAAAGTCCCCGCTGACGATGGACAAGC
TGGTCGCCTTTGGCGCGATCCCCAAGCCGGTCGCCGAATTCATTCTGGGGGCCGTGAAATGCCGGGCATCGACGGTCATT
TCCGGGGGCACGGGCTCTGGCAAGACGACGCTGCTCAATGCGCTTTCGGCGGCGATTAATCCGGACGAGCGCCTCATCAC
CATTGAGGACGCCGCCGAACTTCAACTGCAGCAACCGCATGTGGCACGCATGGAAACGCGCCCGCCGAACATTGAGGGCA
AGGGCGAGATCCGCCAGCGCGAGCTGGTCAAAAACGCCCTGCGGATGCGTCCAGACCGGGTGATCCTCGGCGAGGTGCGC
TCCGAAGAAGCCTTCGACATGTTGCAGGCCATGAATACCGGCCATGAAGGCTCGATGGCGACGATCCACGCCAATAATCC
GCGCGATGCGATCACCCGCCTTGAGCAGATGGTGATGATGGGCGGCATGAAGATCTCTGAAGAGGCCATCCGGGGGCAGA
TAGCTTCGGCGGTGAACTTCATTGTTCAGGCGACACGCCTGTCAGACGGTTCGCGCAAGGTGATTTCGATTGCCGAGATT
ACCGGCATGGAAGGCTCGGTTGTCCAGTTGCAGGAAATCTTCAAGTTCGAGCGGACGGGCACCACGCCGGAAGGCAAGGT
GCAGGGGCATTTTGTGGCGACGGGGCTGCGCCCCAAATTCGTGGATGAAATGGAGCGCAAGGGCGTGTTCATGCCGGCCG
GGCTCTTTGATCCATCCAGCAAATTCTGA

Upstream 100 bases:

>100_bases
GCCTGCTGGTGGCAGATGTTGCCGGGCTGGTGCGCAAGTTTGCGCCGGAAGAAACGCTTGCCGCCTTTGAGGCGAGCCAT
AAAGCGAAGCGAGCGAAACG

Downstream 100 bases:

>100_bases
AGGAGCGGGCGACTGTGTTCGGGCTGCCGGATCTTGCAACTCTCGATATGCGGCTGGTGATCCCGGCGATCATGGCTGTG
GGCGCATTGTTTCTGGCTGT

Product: type II/IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSRFGFTNAAPKVAEAVPAPVAPQAAPAVSPKQTQAEQASFDLLDAKLRVHAKLIDELDLSALDKLDDETMRRRVRGIIS
EIIRKEEMALSAAEEASFADAVMDEMTGLGPIEPLLKDDSIADILINGCNQVYIERGGKLQLAPVRFADNDHLLRIVQRI
VAAVGRRVDESQPLVDARLLDGSRVNAAVAPIAIDGPLVSIRKFSKSPLTMDKLVAFGAIPKPVAEFILGAVKCRASTVI
SGGTGSGKTTLLNALSAAINPDERLITIEDAAELQLQQPHVARMETRPPNIEGKGEIRQRELVKNALRMRPDRVILGEVR
SEEAFDMLQAMNTGHEGSMATIHANNPRDAITRLEQMVMMGGMKISEEAIRGQIASAVNFIVQATRLSDGSRKVISIAEI
TGMEGSVVQLQEIFKFERTGTTPEGKVQGHFVATGLRPKFVDEMERKGVFMPAGLFDPSSKF

Sequences:

>Translated_462_residues
MSRFGFTNAAPKVAEAVPAPVAPQAAPAVSPKQTQAEQASFDLLDAKLRVHAKLIDELDLSALDKLDDETMRRRVRGIIS
EIIRKEEMALSAAEEASFADAVMDEMTGLGPIEPLLKDDSIADILINGCNQVYIERGGKLQLAPVRFADNDHLLRIVQRI
VAAVGRRVDESQPLVDARLLDGSRVNAAVAPIAIDGPLVSIRKFSKSPLTMDKLVAFGAIPKPVAEFILGAVKCRASTVI
SGGTGSGKTTLLNALSAAINPDERLITIEDAAELQLQQPHVARMETRPPNIEGKGEIRQRELVKNALRMRPDRVILGEVR
SEEAFDMLQAMNTGHEGSMATIHANNPRDAITRLEQMVMMGGMKISEEAIRGQIASAVNFIVQATRLSDGSRKVISIAEI
TGMEGSVVQLQEIFKFERTGTTPEGKVQGHFVATGLRPKFVDEMERKGVFMPAGLFDPSSKF
>Mature_461_residues
SRFGFTNAAPKVAEAVPAPVAPQAAPAVSPKQTQAEQASFDLLDAKLRVHAKLIDELDLSALDKLDDETMRRRVRGIISE
IIRKEEMALSAAEEASFADAVMDEMTGLGPIEPLLKDDSIADILINGCNQVYIERGGKLQLAPVRFADNDHLLRIVQRIV
AAVGRRVDESQPLVDARLLDGSRVNAAVAPIAIDGPLVSIRKFSKSPLTMDKLVAFGAIPKPVAEFILGAVKCRASTVIS
GGTGSGKTTLLNALSAAINPDERLITIEDAAELQLQQPHVARMETRPPNIEGKGEIRQRELVKNALRMRPDRVILGEVRS
EEAFDMLQAMNTGHEGSMATIHANNPRDAITRLEQMVMMGGMKISEEAIRGQIASAVNFIVQATRLSDGSRKVISIAEIT
GMEGSVVQLQEIFKFERTGTTPEGKVQGHFVATGLRPKFVDEMERKGVFMPAGLFDPSSKF

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=121, Percent_Identity=34.7107438016529, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50130; Mature: 49998

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFGFTNAAPKVAEAVPAPVAPQAAPAVSPKQTQAEQASFDLLDAKLRVHAKLIDELDL
CCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALDKLDDETMRRRVRGIISEIIRKEEMALSAAEEASFADAVMDEMTGLGPIEPLLKDDS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
IADILINGCNQVYIERGGKLQLAPVRFADNDHLLRIVQRIVAAVGRRVDESQPLVDARLL
HHHHHHHCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEC
DGSRVNAAVAPIAIDGPLVSIRKFSKSPLTMDKLVAFGAIPKPVAEFILGAVKCRASTVI
CCCCCCCEEEEEEECCCHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEE
SGGTGSGKTTLLNALSAAINPDERLITIEDAAELQLQQPHVARMETRPPNIEGKGEIRQR
ECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHEECCCCHHHCCCCCCCCCCCCHHHHH
ELVKNALRMRPDRVILGEVRSEEAFDMLQAMNTGHEGSMATIHANNPRDAITRLEQMVMM
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHH
GGMKISEEAIRGQIASAVNFIVQATRLSDGSRKVISIAEITGMEGSVVQLQEIFKFERTG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
TTPEGKVQGHFVATGLRPKFVDEMERKGVFMPAGLFDPSSKF
CCCCCCCCEEEEEECCCHHHHHHHHHCCEEEECCCCCCCCCC
>Mature Secondary Structure 
SRFGFTNAAPKVAEAVPAPVAPQAAPAVSPKQTQAEQASFDLLDAKLRVHAKLIDELDL
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALDKLDDETMRRRVRGIISEIIRKEEMALSAAEEASFADAVMDEMTGLGPIEPLLKDDS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCC
IADILINGCNQVYIERGGKLQLAPVRFADNDHLLRIVQRIVAAVGRRVDESQPLVDARLL
HHHHHHHCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEC
DGSRVNAAVAPIAIDGPLVSIRKFSKSPLTMDKLVAFGAIPKPVAEFILGAVKCRASTVI
CCCCCCCEEEEEEECCCHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHEEE
SGGTGSGKTTLLNALSAAINPDERLITIEDAAELQLQQPHVARMETRPPNIEGKGEIRQR
ECCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHEECCCCHHHCCCCCCCCCCCCHHHHH
ELVKNALRMRPDRVILGEVRSEEAFDMLQAMNTGHEGSMATIHANNPRDAITRLEQMVMM
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHH
GGMKISEEAIRGQIASAVNFIVQATRLSDGSRKVISIAEITGMEGSVVQLQEIFKFERTG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCC
TTPEGKVQGHFVATGLRPKFVDEMERKGVFMPAGLFDPSSKF
CCCCCCCCEEEEEECCCHHHHHHHHHCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]