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The map label for this gene is iaaA [H]

Identifier: 114799029

GI number: 114799029

Start: 1719404

End: 1720306

Strand: Reverse

Name: iaaA [H]

Synonym: HNE_1675

Alternate gene names: 114799029

Gene position: 1720306-1719404 (Counterclockwise)

Preceding gene: 114798244

Following gene: 114799811

Centisome position: 46.43

GC content: 65.56

Gene sequence:

>903_bases
GTGATCCATGGCGGGGCCGGCGTGATCCTGCCTGGCTCCATGACGCCTGAAATGGAGGCGGCCTATATTGCCGCGCTGAA
CGTCTCGCTTGAGGCGGGCGCCGCCGTGCTGCGCGAAGGCGGCAGCGCGCTTGACGCCGTTGAAGCCGCCGTGCTGACGA
TGGAAGACGACGCGCTGTTCAATGCCGGGCTAGGCGCCGTCTTCACGGCTGAAGGCACGCACGAACTTGACGCCTCCATC
ATGGATGGACGCGACCGGAATGCGGGCGCCGTGGCCGGCGTGATGCGCGTGAAAAATCCGATCAAAGCGGCCCGCGCCGT
CATGGAAAAATCCGAACACGTCATGTTTGCGGCCGAAGGCGCCGATGCGTTTGCGCAGGACCAGGGCCTCGAGATGGTGG
ACAACACATATTTCGATACCGACCGCCGCCGCGAAGCGCTGGACCGTGTCCTGCGCGAGCGGGCCCGCACCGCCGCAGAT
CGTCACGGTACGGTGGGCGCCGTCGCCATGGACCTTGAAGGCAACCTCGCCGCCGCCACCACCACGGGCGGCATGACCGC
CAAAGCCGCAGGCCGCGTGGGCGACGCGCCTTTGATTGGCGCAGCCACCTATGCCGAAAACGGCGTGTGCGCGGTCTCCG
CCACCGGGCATGGCGAATATTTCATCCGCGTCGGCGTTGCCAAAACGATCTGCGACCGCGTGAAACTTGGCGGGGAAGAC
GTGAAGACCGCCGCTGGCGTTGCGCTGGGTGAAGTGGCCGCGCTGGGCGGAGATGGCGGCGTCATCGTGATCGGCGGAAC
GGGCGATTATGGCTTCGTGTTCAACTCCGCCGGCATGTATCGCGGCTGGATCGATGCGTCAGGGTCGGGCACAGCTATCT
ATGGCCAAGACGATGCCGATTAA

Upstream 100 bases:

>100_bases
TGTTGCCCTCGCCCTCAGTGGACTGATCGTGAGCGCCTGCGCCAGTGCCTCTTCTGAGCCAATAGCCGCCCCTGCCTCTG
CGACGCCCGAGTGGCGCCTC

Downstream 100 bases:

>100_bases
TCTGACGTCCCTCAAGGTGCGCGATGCCGGGCCGGGAGACCTTGCCGCGCTCACCGCCCTCTCGCAGAAAACGTTCACGG
ACAAGTTCGGGCAGCTTTAC

Product: putative asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIHGGAGVILPGSMTPEMEAAYIAALNVSLEAGAAVLREGGSALDAVEAAVLTMEDDALFNAGLGAVFTAEGTHELDASI
MDGRDRNAGAVAGVMRVKNPIKAARAVMEKSEHVMFAAEGADAFAQDQGLEMVDNTYFDTDRRREALDRVLRERARTAAD
RHGTVGAVAMDLEGNLAAATTTGGMTAKAAGRVGDAPLIGAATYAENGVCAVSATGHGEYFIRVGVAKTICDRVKLGGED
VKTAAGVALGEVAALGGDGGVIVIGGTGDYGFVFNSAGMYRGWIDASGSGTAIYGQDDAD

Sequences:

>Translated_300_residues
MIHGGAGVILPGSMTPEMEAAYIAALNVSLEAGAAVLREGGSALDAVEAAVLTMEDDALFNAGLGAVFTAEGTHELDASI
MDGRDRNAGAVAGVMRVKNPIKAARAVMEKSEHVMFAAEGADAFAQDQGLEMVDNTYFDTDRRREALDRVLRERARTAAD
RHGTVGAVAMDLEGNLAAATTTGGMTAKAAGRVGDAPLIGAATYAENGVCAVSATGHGEYFIRVGVAKTICDRVKLGGED
VKTAAGVALGEVAALGGDGGVIVIGGTGDYGFVFNSAGMYRGWIDASGSGTAIYGQDDAD
>Mature_300_residues
MIHGGAGVILPGSMTPEMEAAYIAALNVSLEAGAAVLREGGSALDAVEAAVLTMEDDALFNAGLGAVFTAEGTHELDASI
MDGRDRNAGAVAGVMRVKNPIKAARAVMEKSEHVMFAAEGADAFAQDQGLEMVDNTYFDTDRRREALDRVLRERARTAAD
RHGTVGAVAMDLEGNLAAATTTGGMTAKAAGRVGDAPLIGAATYAENGVCAVSATGHGEYFIRVGVAKTICDRVKLGGED
VKTAAGVALGEVAALGGDGGVIVIGGTGDYGFVFNSAGMYRGWIDASGSGTAIYGQDDAD

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=272, Percent_Identity=43.75, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI145275200, Length=272, Percent_Identity=43.75, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI98991769, Length=265, Percent_Identity=30.9433962264151, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI285002251, Length=284, Percent_Identity=29.5774647887324, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI285002253, Length=284, Percent_Identity=27.8169014084507, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787050, Length=292, Percent_Identity=49.3150684931507, Blast_Score=245, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI32564649, Length=209, Percent_Identity=24.4019138755981, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18921183, Length=319, Percent_Identity=38.5579937304075, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21357893, Length=266, Percent_Identity=31.5789473684211, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI221330102, Length=237, Percent_Identity=32.0675105485232, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652135, Length=237, Percent_Identity=32.0675105485232, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 30494; Mature: 30494

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHGGAGVILPGSMTPEMEAAYIAALNVSLEAGAAVLREGGSALDAVEAAVLTMEDDALF
CEECCCCEEECCCCCCCCCEEEEEEEEEEHHHHHHHHHCCCCHHHHHHEEEEEECCCHHC
NAGLGAVFTAEGTHELDASIMDGRDRNAGAVAGVMRVKNPIKAARAVMEKSEHVMFAAEG
CCCCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECC
ADAFAQDQGLEMVDNTYFDTDRRREALDRVLRERARTAADRHGTVGAVAMDLEGNLAAAT
CCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
TTGGMTAKAAGRVGDAPLIGAATYAENGVCAVSATGHGEYFIRVGVAKTICDRVKLGGED
ECCCCEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEECHHHHHHHHHHCCCCH
VKTAAGVALGEVAALGGDGGVIVIGGTGDYGFVFNSAGMYRGWIDASGSGTAIYGQDDAD
HHHHHCHHHHHHEEECCCCCEEEEECCCCCCEEECCCCCEEEEEECCCCCCEEECCCCCC
>Mature Secondary Structure
MIHGGAGVILPGSMTPEMEAAYIAALNVSLEAGAAVLREGGSALDAVEAAVLTMEDDALF
CEECCCCEEECCCCCCCCCEEEEEEEEEEHHHHHHHHHCCCCHHHHHHEEEEEECCCHHC
NAGLGAVFTAEGTHELDASIMDGRDRNAGAVAGVMRVKNPIKAARAVMEKSEHVMFAAEG
CCCCCEEEECCCCCCCCCHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEECC
ADAFAQDQGLEMVDNTYFDTDRRREALDRVLRERARTAADRHGTVGAVAMDLEGNLAAAT
CCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
TTGGMTAKAAGRVGDAPLIGAATYAENGVCAVSATGHGEYFIRVGVAKTICDRVKLGGED
ECCCCEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCEEEEEECHHHHHHHHHHCCCCH
VKTAAGVALGEVAALGGDGGVIVIGGTGDYGFVFNSAGMYRGWIDASGSGTAIYGQDDAD
HHHHHCHHHHHHEEECCCCCEEEEECCCCCCEEECCCCCEEEEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]