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The map label for this gene is mcrA [H]

Identifier: 114798868

GI number: 114798868

Start: 2681872

End: 2682903

Strand: Reverse

Name: mcrA [H]

Synonym: HNE_2533

Alternate gene names: 114798868

Gene position: 2682903-2681872 (Counterclockwise)

Preceding gene: 114799606

Following gene: 114797384

Centisome position: 72.41

GC content: 54.65

Gene sequence:

>1032_bases
GTGGTGCATGAGGGGGAAGCGTTGCCGTCAAAGGCGATCCTCGCTGGCGCGTATTTTCATCAGCATGGAGCAGACATCGG
CAAGTTTGTCGGTGGCGCGGGTGTTGCGCGCCAATTACAAAAGCTAGGGTTTGAAATGATCATCCGACGGGGCGGTAAGG
ACGTTCCAATCGGAGAGATCTTCGAGAATGAAACGCCTCACGGACATTCCTTTCGCATCGGTGCGCACTATAGTCGGCGC
GCGGATATTCATGAAGTGTATGGCGGACAAATGCAGGGCGGGATTTCAACGCCGGCTGATGCGCCCTTTGTCTTTATCTT
TACAGGCGACGCCGGCGAACAGCATGGATACCGCGACGGCTGGCAGGAAGACCGTGAGACCTTCCTCTATACCGGGGAAG
GTCAACGGGGCGATATGACCTTTAAAAGAGGGAATCGCGCGATCCAAGAGCATGCCACTGACGGGAAGGCCATTTTGCTT
TTCGAAGCCCTCGGCAAAGGCAAACTATACGAATTTATGGGAGAGTTTGTCTGTGCGGGCTGGGAGATGATCGACTCTCA
CGACATCGACAAGCTGGAGCGTAAGGCCATTCAGTTTCATCTCGTGCGGGCCGATGCTGTCGCTGATTCTGAAACAGACG
AAGAAATTGAAGATCAACCTGATACGTCTATCGATGATCTGCGGACGTCTGCCTATGAGGCGGCGACCGCGGTCAGAAAT
TCTAATCCCAAGGAAGCGCGGCGAGTTTATCGTCAGCGCAGTGCAAAGATTAAAGCCTACATTCTCGCGCGAGCAGGCGG
CGTCTGTGAACTGACGGGTGAGAAAGCCCCGTTCCTCACAAAATCAGGCCATCCCTATCTGGAAGTCCATCATACACAGC
GGCTGTCAGACGATGGGCTCGACCACCCCCGCTGGGTCGCTGCCATTAGTCCGACGGCTCACCGAGAGATCCATTTCGGT
GAGCGTGGCGATGAGCTCAATGAACGTCTCAAAGAGATAATCGCGGAGAAAGAAAAGTCGATCGCGCGCTAA

Upstream 100 bases:

>100_bases
CACAGAGCCGCAGGCCGTCCTCGATGCGATCGCAGAATATCGCCGCGGTCCGGACGCTTTCCTCCAGAAATACAAACGCG
GGGAAGCGAGGGAGTACTAT

Downstream 100 bases:

>100_bases
GATTGCTATTCACGTTCGGTAATGGCTCCATCGACGCGTGCAGAAAGTCTGATTCGGCGTGAGCGTGCCGCCTGGTGGGA
AGATCAGTAGACAATGCAAG

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVHEGEALPSKAILAGAYFHQHGADIGKFVGGAGVARQLQKLGFEMIIRRGGKDVPIGEIFENETPHGHSFRIGAHYSRR
ADIHEVYGGQMQGGISTPADAPFVFIFTGDAGEQHGYRDGWQEDRETFLYTGEGQRGDMTFKRGNRAIQEHATDGKAILL
FEALGKGKLYEFMGEFVCAGWEMIDSHDIDKLERKAIQFHLVRADAVADSETDEEIEDQPDTSIDDLRTSAYEAATAVRN
SNPKEARRVYRQRSAKIKAYILARAGGVCELTGEKAPFLTKSGHPYLEVHHTQRLSDDGLDHPRWVAAISPTAHREIHFG
ERGDELNERLKEIIAEKEKSIAR

Sequences:

>Translated_343_residues
MVHEGEALPSKAILAGAYFHQHGADIGKFVGGAGVARQLQKLGFEMIIRRGGKDVPIGEIFENETPHGHSFRIGAHYSRR
ADIHEVYGGQMQGGISTPADAPFVFIFTGDAGEQHGYRDGWQEDRETFLYTGEGQRGDMTFKRGNRAIQEHATDGKAILL
FEALGKGKLYEFMGEFVCAGWEMIDSHDIDKLERKAIQFHLVRADAVADSETDEEIEDQPDTSIDDLRTSAYEAATAVRN
SNPKEARRVYRQRSAKIKAYILARAGGVCELTGEKAPFLTKSGHPYLEVHHTQRLSDDGLDHPRWVAAISPTAHREIHFG
ERGDELNERLKEIIAEKEKSIAR
>Mature_343_residues
MVHEGEALPSKAILAGAYFHQHGADIGKFVGGAGVARQLQKLGFEMIIRRGGKDVPIGEIFENETPHGHSFRIGAHYSRR
ADIHEVYGGQMQGGISTPADAPFVFIFTGDAGEQHGYRDGWQEDRETFLYTGEGQRGDMTFKRGNRAIQEHATDGKAILL
FEALGKGKLYEFMGEFVCAGWEMIDSHDIDKLERKAIQFHLVRADAVADSETDEEIEDQPDTSIDDLRTSAYEAATAVRN
SNPKEARRVYRQRSAKIKAYILARAGGVCELTGEKAPFLTKSGHPYLEVHHTQRLSDDGLDHPRWVAAISPTAHREIHFG
ERGDELNERLKEIIAEKEKSIAR

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=130, Percent_Identity=33.8461538461538, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 38241; Mature: 38241

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHEGEALPSKAILAGAYFHQHGADIGKFVGGAGVARQLQKLGFEMIIRRGGKDVPIGEI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
FENETPHGHSFRIGAHYSRRADIHEVYGGQMQGGISTPADAPFVFIFTGDAGEQHGYRDG
HCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
WQEDRETFLYTGEGQRGDMTFKRGNRAIQEHATDGKAILLFEALGKGKLYEFMGEFVCAG
CHHCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHC
WEMIDSHDIDKLERKAIQFHLVRADAVADSETDEEIEDQPDTSIDDLRTSAYEAATAVRN
HHHHCCCCHHHHHHHHHHHEEEEHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHC
SNPKEARRVYRQRSAKIKAYILARAGGVCELTGEKAPFLTKSGHPYLEVHHTQRLSDDGL
CCHHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCEECCCCCEEEEECCHHCCCCCC
DHPRWVAAISPTAHREIHFGERGDELNERLKEIIAEKEKSIAR
CCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVHEGEALPSKAILAGAYFHQHGADIGKFVGGAGVARQLQKLGFEMIIRRGGKDVPIGEI
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
FENETPHGHSFRIGAHYSRRADIHEVYGGQMQGGISTPADAPFVFIFTGDAGEQHGYRDG
HCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
WQEDRETFLYTGEGQRGDMTFKRGNRAIQEHATDGKAILLFEALGKGKLYEFMGEFVCAG
CHHCCCEEEEECCCCCCCCCHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHC
WEMIDSHDIDKLERKAIQFHLVRADAVADSETDEEIEDQPDTSIDDLRTSAYEAATAVRN
HHHHCCCCHHHHHHHHHHHEEEEHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHC
SNPKEARRVYRQRSAKIKAYILARAGGVCELTGEKAPFLTKSGHPYLEVHHTQRLSDDGL
CCHHHHHHHHHHHCCCEEEEEEEECCCEEEECCCCCCCEECCCCCEEEEECCHHCCCCCC
DHPRWVAAISPTAHREIHFGERGDELNERLKEIIAEKEKSIAR
CCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]