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The map label for this gene is gcvA [H]

Identifier: 114798673

GI number: 114798673

Start: 316818

End: 317750

Strand: Reverse

Name: gcvA [H]

Synonym: HNE_0321

Alternate gene names: 114798673

Gene position: 317750-316818 (Counterclockwise)

Preceding gene: 114799577

Following gene: 114800541

Centisome position: 8.58

GC content: 65.92

Gene sequence:

>933_bases
ATGTCTGACTCCTCCGATCGCCTGCCCCCGCTCAACGCCCTGCGCGCCTTCGAGGCGGCTGCACGCCGCCTGTCGTTCAC
CCAGGCGGCCGAGGAGCTGAATGTCACACCCGGCGCAATTTCGCAGCAGATCCGCCAGCTGGAGGACTTTGCCGGGACGC
CGCTGTTCAAGCGTACGGGGCGTTCCGTTCTGCTGACCGACGCCGCGCAGGCGAGCCTGCCGCTGGTGCGCGAGGCGTTT
GAGCGGATCGCCGAGGCGGGCCGCGTGATGCAGGCCCCTGCCCGCAAGGGGCGGGTGATGATCTCGTCAGCGCCCTCGTT
TGCCGCAAAATGGCTGGCGCCGCGCCTGGAGCGGTTTCAGCAGCAGAATGAAGGCATCGAGGCCTGGATTTCGGCCGACA
TGTCGCTGACCGATTTCAACACCGCGGACGCCGACATCGCCATCCGCTATGGGCGGGGCAATTATGACGGGCTGAAATCG
GAAAAGCTGATGGATGAGACGGTACTGCCCGTCTGCGCGCCGCGCCTGCTGGAAGGGCCGAACGCCATCCTGAAGCCCGA
AGACCTGCGCCATCATACGCTGCTGCATGATGAAAGCTCGGAAAACGACCCCTCCTGCCCGGACTGGACAAGCTGGCTGC
GCGCACATGGCGTGACCGGCGTGGAGGGCAGCCGGGGGCCGCGCTTCAATCAGGGCATCCTGGTCATCGAGTCGGCCGCG
GCCGGGCGGGGTGTCGCGCTGGCCAAGCAGGCGATTGCGGCTGCCGATCTGGAAGCCGGGCGCCTCGTGGCGCCGTTTGC
CAATGGATCGATCCCGATTGATTTTGGTTATTGGCTCGTCTGGCCGAAGGGGCGTCACCTCTCGCCGGAAGTGCGCGCCT
TCATCAAATGGATCAAGGACGAAGCGGCCAGCAGCGAAGTGGTCGGCGTTTAG

Upstream 100 bases:

>100_bases
CTTTCTTATTAGAGACACTACAGGGACGCATATACAAAACCTCCTTGACGCCTCAGGCGCAACGGGCAACCCCTACAGCG
ATCATTTAGAGAAACTATCC

Downstream 100 bases:

>100_bases
ACCCGCAGCCTAGAGGGCAGCGTCCAGCAACAGGAAAGCAGCAAGGCGCGGGCTGGCATTGCGGCTGGTGCTCTGGGTCT
GCTCAAGCGCAGCCAGGGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSDSSDRLPPLNALRAFEAAARRLSFTQAAEELNVTPGAISQQIRQLEDFAGTPLFKRTGRSVLLTDAAQASLPLVREAF
ERIAEAGRVMQAPARKGRVMISSAPSFAAKWLAPRLERFQQQNEGIEAWISADMSLTDFNTADADIAIRYGRGNYDGLKS
EKLMDETVLPVCAPRLLEGPNAILKPEDLRHHTLLHDESSENDPSCPDWTSWLRAHGVTGVEGSRGPRFNQGILVIESAA
AGRGVALAKQAIAAADLEAGRLVAPFANGSIPIDFGYWLVWPKGRHLSPEVRAFIKWIKDEAASSEVVGV

Sequences:

>Translated_310_residues
MSDSSDRLPPLNALRAFEAAARRLSFTQAAEELNVTPGAISQQIRQLEDFAGTPLFKRTGRSVLLTDAAQASLPLVREAF
ERIAEAGRVMQAPARKGRVMISSAPSFAAKWLAPRLERFQQQNEGIEAWISADMSLTDFNTADADIAIRYGRGNYDGLKS
EKLMDETVLPVCAPRLLEGPNAILKPEDLRHHTLLHDESSENDPSCPDWTSWLRAHGVTGVEGSRGPRFNQGILVIESAA
AGRGVALAKQAIAAADLEAGRLVAPFANGSIPIDFGYWLVWPKGRHLSPEVRAFIKWIKDEAASSEVVGV
>Mature_309_residues
SDSSDRLPPLNALRAFEAAARRLSFTQAAEELNVTPGAISQQIRQLEDFAGTPLFKRTGRSVLLTDAAQASLPLVREAFE
RIAEAGRVMQAPARKGRVMISSAPSFAAKWLAPRLERFQQQNEGIEAWISADMSLTDFNTADADIAIRYGRGNYDGLKSE
KLMDETVLPVCAPRLLEGPNAILKPEDLRHHTLLHDESSENDPSCPDWTSWLRAHGVTGVEGSRGPRFNQGILVIESAAA
GRGVALAKQAIAAADLEAGRLVAPFANGSIPIDFGYWLVWPKGRHLSPEVRAFIKWIKDEAASSEVVGV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=45.0331125827815, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=33.1058020477816, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=30.0675675675676, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=25.3378378378378, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=21.830985915493, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=24.8299319727891, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33779; Mature: 33647

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSSDRLPPLNALRAFEAAARRLSFTQAAEELNVTPGAISQQIRQLEDFAGTPLFKRTG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCC
RSVLLTDAAQASLPLVREAFERIAEAGRVMQAPARKGRVMISSAPSFAAKWLAPRLERFQ
CEEEEECCHHHHHHHHHHHHHHHHHHCHHHHCHHHCCEEEEECCCHHHHHHHHHHHHHHH
QQNEGIEAWISADMSLTDFNTADADIAIRYGRGNYDGLKSEKLMDETVLPVCAPRLLEGP
HHCCCCEEEHCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NAILKPEDLRHHTLLHDESSENDPSCPDWTSWLRAHGVTGVEGSRGPRFNQGILVIESAA
CCCCCCCHHHHHHEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
AGRGVALAKQAIAAADLEAGRLVAPFANGSIPIDFGYWLVWPKGRHLSPEVRAFIKWIKD
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECEEEEECCCCCCCHHHHHHHHHHHH
EAASSEVVGV
HHCCCCCCCC
>Mature Secondary Structure 
SDSSDRLPPLNALRAFEAAARRLSFTQAAEELNVTPGAISQQIRQLEDFAGTPLFKRTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCC
RSVLLTDAAQASLPLVREAFERIAEAGRVMQAPARKGRVMISSAPSFAAKWLAPRLERFQ
CEEEEECCHHHHHHHHHHHHHHHHHHCHHHHCHHHCCEEEEECCCHHHHHHHHHHHHHHH
QQNEGIEAWISADMSLTDFNTADADIAIRYGRGNYDGLKSEKLMDETVLPVCAPRLLEGP
HHCCCCEEEHCCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NAILKPEDLRHHTLLHDESSENDPSCPDWTSWLRAHGVTGVEGSRGPRFNQGILVIESAA
CCCCCCCHHHHHHEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCC
AGRGVALAKQAIAAADLEAGRLVAPFANGSIPIDFGYWLVWPKGRHLSPEVRAFIKWIKD
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEECEEEEECCCCCCCHHHHHHHHHHHH
EAASSEVVGV
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]