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The map label for this gene is hyfB [H]

Identifier: 114798610

GI number: 114798610

Start: 748881

End: 750656

Strand: Direct

Name: hyfB [H]

Synonym: HNE_0738

Alternate gene names: 114798610

Gene position: 748881-750656 (Clockwise)

Preceding gene: 114798330

Following gene: 114799831

Centisome position: 20.21

GC content: 60.47

Gene sequence:

>1776_bases
ATGCCGGCCTGGCTTGCAGCCCTCAATCCCGGCTGGCTGATGATCCTTGTGGGTCTCGTCGCCCTGGTTCTCCCATACCG
CAAGGTGCGTCAGACCCTGACGGTCGGAACGCCGATCCTCGCGATCGCCATGCTTGTAATGGCGCCGCATGAGGTGGACC
TGCTGACCGCAGAAGCGCTGGACATCACCTTCAGCTTCTACCGCGTCGACAGCCTGAACTTCATTTTCGGTCTGGCCTTC
CTGATCGCCTCGCTGCTGAACGCGATCTACGCCCTGCATACGAACAACCCGCGCCAGGACGGCATGGCGCTCGCCTATGC
TGGCGCAGCAGTCTCTGCGTCGTTCTCCGGCGACCTGATGACGCTGTTCGTTTTCTGGGAGCTTACCGCCATCACGTCGG
TCTTCCTGATCCTGCAGGCGGGCACCCGCGCGGCGTATTTTGCGTCCATGCGCTATCTGGTGATCCAGATCCTGTCAGGC
GTGATCCTGCTCGACGGCCTTTCTTATGTCTGGCGCTACAATGGAACGCTCTCGCTTGAGGCCTTCCAGAGCTTCAGCGA
TCCCGGCGCGATCTACATTTTCATCGCACTGGGCATCAAGGCAGCGTTTCCGTTCATGCATAACTGGCTGCAGGATTCCT
ATCCCAAGTCATCCGTGGTCGGCGCCGTGGTCCTCTCGGCCTTTACAACCAAGCTCGCGGTCTATGGCTTCGCGCGCCTG
TTTCCAGGTTTCGATGCGCTGATCTGGATCGGCGCGATCATGACTATCTTCCCGGTCTTCTTCGCCGTCATCGAGAATGA
TCTGCGCCGGGTTCTTGCGTATTCGCTGAATAACCAGGTCGGCTTCATGATCTGCGCCATCGGCGTCGGGGCAGTTGGTG
TGGGCAATCCTCTGGCGCTGAACGGCGCCAGCGCGCACGCCTTCGCGCACATCATCTACAAGGCGCTGCTATTCATGAGC
ATGGGCGCGGTCCTCTACCGGACTGGCACGACCAAAGCCTCTGAGCTGGGCGGGCTTTATCGCTCCATGCCGTGGACGAC
GCTGTTCTGCATCATTGGTGGTCTGTCGATTTCGGCCTTCCCGCTCTTCTCTGGTTTTGTCGCCAAATCCCTGACGATGA
GCGCGGTTGCCTATGAAGGCGTGTCCGATGCCGGCATGGTCGTGGTCTGGTTGATGTTGCTGTTTGCCTCTGCGGGCGTG
CTCGAACACTCGGGCATCAAGATCCCGTACTTTGCCTTCTTCGGCCACGACAGCGGCAAGCGGGTGAAGGAAGCGCCCTT
CAACATGCTGCTTGCTATGGGCATGGCGGCGTTCCTGTGTATTGCGGTCGGCATGCCTGCGGTGATCCCCGGCTTTGGCT
ATCAATGGCTCTACAGCCTGCTGCCGTATCCGATAGAGGCGTCGAGTTATCAGCCCTTCACGCTGGAACACATTCTCACG
CAGATGCAGCTCCTCGTGCTCGCCACGTTCGCCTTCATCCTACTCAAACGCTTCGCGCTGTACCCGGCGGAGAAGCCCGG
CACCATTCTCGATACAGACTGGCTCTACCGGAAGGCTGGCTACGGGTTCGTTCAATGGTCGGGCACGGTTTGGGGCAAGA
TGGGGCCTGCGATCTCGGCGATGTTCGGGCGCGTCTCTGACAGGGCCTACAAACGGCTGGAATCGGCTTTCAGTCCGCGC
GGCGACCTGGCGCGTGGCGGCCTGTCCGGCGGCGCAGCGGTGTGGACTGCGGTTCTTCTGGCTGCGGTTCTGCTGCTTTC
CTTTCTGTCGAGCTGA

Upstream 100 bases:

>100_bases
ACTATTACGGTGACCTGAGTGGACCGCCGGCAGACATTGATCCGGCGATTGCTGACCGTCTGAACGCCGGCGAGCCTAAA
GCCTTTGTGGGAGAAGAATG

Downstream 100 bases:

>100_bases
CAATCCGGCCGGCCAGCGCGATTTGCTGGCCGGTTTCCGGGGACCGGCGCTGATGCAGTGATGCAATAAAGCCTCAATGT
TGAGGGCCGGACGGCCAGTC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MPAWLAALNPGWLMILVGLVALVLPYRKVRQTLTVGTPILAIAMLVMAPHEVDLLTAEALDITFSFYRVDSLNFIFGLAF
LIASLLNAIYALHTNNPRQDGMALAYAGAAVSASFSGDLMTLFVFWELTAITSVFLILQAGTRAAYFASMRYLVIQILSG
VILLDGLSYVWRYNGTLSLEAFQSFSDPGAIYIFIALGIKAAFPFMHNWLQDSYPKSSVVGAVVLSAFTTKLAVYGFARL
FPGFDALIWIGAIMTIFPVFFAVIENDLRRVLAYSLNNQVGFMICAIGVGAVGVGNPLALNGASAHAFAHIIYKALLFMS
MGAVLYRTGTTKASELGGLYRSMPWTTLFCIIGGLSISAFPLFSGFVAKSLTMSAVAYEGVSDAGMVVVWLMLLFASAGV
LEHSGIKIPYFAFFGHDSGKRVKEAPFNMLLAMGMAAFLCIAVGMPAVIPGFGYQWLYSLLPYPIEASSYQPFTLEHILT
QMQLLVLATFAFILLKRFALYPAEKPGTILDTDWLYRKAGYGFVQWSGTVWGKMGPAISAMFGRVSDRAYKRLESAFSPR
GDLARGGLSGGAAVWTAVLLAAVLLLSFLSS

Sequences:

>Translated_591_residues
MPAWLAALNPGWLMILVGLVALVLPYRKVRQTLTVGTPILAIAMLVMAPHEVDLLTAEALDITFSFYRVDSLNFIFGLAF
LIASLLNAIYALHTNNPRQDGMALAYAGAAVSASFSGDLMTLFVFWELTAITSVFLILQAGTRAAYFASMRYLVIQILSG
VILLDGLSYVWRYNGTLSLEAFQSFSDPGAIYIFIALGIKAAFPFMHNWLQDSYPKSSVVGAVVLSAFTTKLAVYGFARL
FPGFDALIWIGAIMTIFPVFFAVIENDLRRVLAYSLNNQVGFMICAIGVGAVGVGNPLALNGASAHAFAHIIYKALLFMS
MGAVLYRTGTTKASELGGLYRSMPWTTLFCIIGGLSISAFPLFSGFVAKSLTMSAVAYEGVSDAGMVVVWLMLLFASAGV
LEHSGIKIPYFAFFGHDSGKRVKEAPFNMLLAMGMAAFLCIAVGMPAVIPGFGYQWLYSLLPYPIEASSYQPFTLEHILT
QMQLLVLATFAFILLKRFALYPAEKPGTILDTDWLYRKAGYGFVQWSGTVWGKMGPAISAMFGRVSDRAYKRLESAFSPR
GDLARGGLSGGAAVWTAVLLAAVLLLSFLSS
>Mature_590_residues
PAWLAALNPGWLMILVGLVALVLPYRKVRQTLTVGTPILAIAMLVMAPHEVDLLTAEALDITFSFYRVDSLNFIFGLAFL
IASLLNAIYALHTNNPRQDGMALAYAGAAVSASFSGDLMTLFVFWELTAITSVFLILQAGTRAAYFASMRYLVIQILSGV
ILLDGLSYVWRYNGTLSLEAFQSFSDPGAIYIFIALGIKAAFPFMHNWLQDSYPKSSVVGAVVLSAFTTKLAVYGFARLF
PGFDALIWIGAIMTIFPVFFAVIENDLRRVLAYSLNNQVGFMICAIGVGAVGVGNPLALNGASAHAFAHIIYKALLFMSM
GAVLYRTGTTKASELGGLYRSMPWTTLFCIIGGLSISAFPLFSGFVAKSLTMSAVAYEGVSDAGMVVVWLMLLFASAGVL
EHSGIKIPYFAFFGHDSGKRVKEAPFNMLLAMGMAAFLCIAVGMPAVIPGFGYQWLYSLLPYPIEASSYQPFTLEHILTQ
MQLLVLATFAFILLKRFALYPAEKPGTILDTDWLYRKAGYGFVQWSGTVWGKMGPAISAMFGRVSDRAYKRLESAFSPRG
DLARGGLSGGAAVWTAVLLAAVLLLSFLSS

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=173, Percent_Identity=26.0115606936416, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=28.2122905027933, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI2367154, Length=475, Percent_Identity=25.0526315789474, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788613, Length=267, Percent_Identity=26.5917602996255, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788831, Length=287, Percent_Identity=26.1324041811847, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788829, Length=290, Percent_Identity=26.2068965517241, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788614, Length=285, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 64062; Mature: 63931

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAWLAALNPGWLMILVGLVALVLPYRKVRQTLTVGTPILAIAMLVMAPHEVDLLTAEAL
CCCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCHHEEEHHHH
DITFSFYRVDSLNFIFGLAFLIASLLNAIYALHTNNPRQDGMALAYAGAAVSASFSGDLM
HHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHCCCCCCCHH
TLFVFWELTAITSVFLILQAGTRAAYFASMRYLVIQILSGVILLDGLSYVWRYNGTLSLE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEHH
AFQSFSDPGAIYIFIALGIKAAFPFMHNWLQDSYPKSSVVGAVVLSAFTTKLAVYGFARL
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPGFDALIWIGAIMTIFPVFFAVIENDLRRVLAYSLNNQVGFMICAIGVGAVGVGNPLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEHHCCCCCCCCCCEE
NGASAHAFAHIIYKALLFMSMGAVLYRTGTTKASELGGLYRSMPWTTLFCIIGGLSISAF
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHH
PLFSGFVAKSLTMSAVAYEGVSDAGMVVVWLMLLFASAGVLEHSGIKIPYFAFFGHDSGK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCC
RVKEAPFNMLLAMGMAAFLCIAVGMPAVIPGFGYQWLYSLLPYPIEASSYQPFTLEHILT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
QMQLLVLATFAFILLKRFALYPAEKPGTILDTDWLYRKAGYGFVQWSGTVWGKMGPAISA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHCCCCEEEECCEECCCCCHHHHH
MFGRVSDRAYKRLESAFSPRGDLARGGLSGGAAVWTAVLLAAVLLLSFLSS
HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAWLAALNPGWLMILVGLVALVLPYRKVRQTLTVGTPILAIAMLVMAPHEVDLLTAEAL
CCCEEECCCCHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCHHEEEHHHH
DITFSFYRVDSLNFIFGLAFLIASLLNAIYALHTNNPRQDGMALAYAGAAVSASFSGDLM
HHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHCCCCCCCHH
TLFVFWELTAITSVFLILQAGTRAAYFASMRYLVIQILSGVILLDGLSYVWRYNGTLSLE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEHH
AFQSFSDPGAIYIFIALGIKAAFPFMHNWLQDSYPKSSVVGAVVLSAFTTKLAVYGFARL
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FPGFDALIWIGAIMTIFPVFFAVIENDLRRVLAYSLNNQVGFMICAIGVGAVGVGNPLAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEHHCCCCCCCCCCEE
NGASAHAFAHIIYKALLFMSMGAVLYRTGTTKASELGGLYRSMPWTTLFCIIGGLSISAF
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCHHHH
PLFSGFVAKSLTMSAVAYEGVSDAGMVVVWLMLLFASAGVLEHSGIKIPYFAFFGHDSGK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCC
RVKEAPFNMLLAMGMAAFLCIAVGMPAVIPGFGYQWLYSLLPYPIEASSYQPFTLEHILT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHH
QMQLLVLATFAFILLKRFALYPAEKPGTILDTDWLYRKAGYGFVQWSGTVWGKMGPAISA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHCCCCEEEECCEECCCCCHHHHH
MFGRVSDRAYKRLESAFSPRGDLARGGLSGGAAVWTAVLLAAVLLLSFLSS
HHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]