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The map label for this gene is paaF [C]

Identifier: 114798546

GI number: 114798546

Start: 1311874

End: 1312674

Strand: Direct

Name: paaF [C]

Synonym: HNE_1282

Alternate gene names: 114798546

Gene position: 1311874-1312674 (Clockwise)

Preceding gene: 114798548

Following gene: 114797387

Centisome position: 35.41

GC content: 64.04

Gene sequence:

>801_bases
ATGCGTGACGCGGTATATGATCTGATCCGGTTTGAAGCGACCGAAGAAGGGCTGGCGGTTGTGACGCTGCACCGGCCCGA
TGTGCACAACGCCTTCAATGCCGAGCTGATTGCCGAGCTGACAGACGTGTTCACGATGATTTCGGAACAGCCGAGCATCC
GCATGATGATCCTGCGCGGGGAGGGAAAATCCTTCTCGGCGGGCGCCGATCTCAACTGGATGGAGCGCGCGTCGACCCAT
TCGCGCGAGGACAACGAGACCGACGCGATGCGCCTGGCCGATATGCTGCGCCGCCTTTACGAGATGCCGCAGATGACGCT
GGCGCTGGTGCAGGGCGCGGCGATGGGCGGGGGCGCGGGGCTGGTGGCGGCGTGCGATGTGGCGATCGCCAAGGCAGGCA
CGCAGTTCCGCTTTTCAGAGGTTCGTCTGGGCCTCACCCCGGCAACGATCAGCCCGTTCGTGATCCAGGCCATTGGCCCG
CGCTGGGCGAAGGCCCTGTTCGTGACCGCGGATGGCTTCGATGCGGCGTATGCGGAGAAAATCGGCCTGGTGCATTATGT
GGCCGAGACCGATGAGCAGATGGGCGAGTTGCAGGAGCATATCGCGCGGCTGGTCTTTTCTGCGGCGCCCGGCGCGATTG
CCGATGCCAAACAGCTGGTCGCCGATGTGACCGGGATGGAGATCGACAATTCCCTGATGCACACGACCGCCAAGCGCATC
GCGGCGCGGCGTGTGTCCGATGAGGGCAAGGAGGGCATTGCCGCTTTCCTCGAAAAGCGCACGCCCTCCTGGAAGGCGTA
G

Upstream 100 bases:

>100_bases
GGCCAGCCTTGCGCTGTGGCACCTTGTGGAGAAGCCCGCCTTGCGCCCATCGAGCCACTATCGCAGGGTGGCGGCAGAGA
CTTGAGAACAGGAAATTCCC

Downstream 100 bases:

>100_bases
AGCCGCGCAGGCCTCGTCAGTTTCTGGCGAGGTCTACGCTCATGCCATACTTGGCCAATGTGACTTCGAGCGCGGTGACG
GGGTTTACCCCGGTCAATTG

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRDAVYDLIRFEATEEGLAVVTLHRPDVHNAFNAELIAELTDVFTMISEQPSIRMMILRGEGKSFSAGADLNWMERASTH
SREDNETDAMRLADMLRRLYEMPQMTLALVQGAAMGGGAGLVAACDVAIAKAGTQFRFSEVRLGLTPATISPFVIQAIGP
RWAKALFVTADGFDAAYAEKIGLVHYVAETDEQMGELQEHIARLVFSAAPGAIADAKQLVADVTGMEIDNSLMHTTAKRI
AARRVSDEGKEGIAAFLEKRTPSWKA

Sequences:

>Translated_266_residues
MRDAVYDLIRFEATEEGLAVVTLHRPDVHNAFNAELIAELTDVFTMISEQPSIRMMILRGEGKSFSAGADLNWMERASTH
SREDNETDAMRLADMLRRLYEMPQMTLALVQGAAMGGGAGLVAACDVAIAKAGTQFRFSEVRLGLTPATISPFVIQAIGP
RWAKALFVTADGFDAAYAEKIGLVHYVAETDEQMGELQEHIARLVFSAAPGAIADAKQLVADVTGMEIDNSLMHTTAKRI
AARRVSDEGKEGIAAFLEKRTPSWKA
>Mature_266_residues
MRDAVYDLIRFEATEEGLAVVTLHRPDVHNAFNAELIAELTDVFTMISEQPSIRMMILRGEGKSFSAGADLNWMERASTH
SREDNETDAMRLADMLRRLYEMPQMTLALVQGAAMGGGAGLVAACDVAIAKAGTQFRFSEVRLGLTPATISPFVIQAIGP
RWAKALFVTADGFDAAYAEKIGLVHYVAETDEQMGELQEHIARLVFSAAPGAIADAKQLVADVTGMEIDNSLMHTTAKRI
AARRVSDEGKEGIAAFLEKRTPSWKA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI157694516, Length=192, Percent_Identity=28.6458333333333, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI213417737, Length=192, Percent_Identity=28.6458333333333, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=27.0588235294118, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI157694520, Length=145, Percent_Identity=31.7241379310345, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=25.296442687747, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI70995211, Length=154, Percent_Identity=30.5194805194805, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=27.9693486590038, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=29.5275590551181, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=28.4584980237154, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=25.2830188679245, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=29.2, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=27.3809523809524, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=269, Percent_Identity=27.1375464684015, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=269, Percent_Identity=24.5353159851301, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=267, Percent_Identity=27.7153558052434, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=28.9719626168224, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=24, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=27.8008298755187, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=29.3893129770992, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129971, Length=267, Percent_Identity=28.4644194756554, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653477, Length=267, Percent_Identity=28.4644194756554, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922422, Length=275, Percent_Identity=22.5454545454545, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28974; Mature: 28974

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAVYDLIRFEATEEGLAVVTLHRPDVHNAFNAELIAELTDVFTMISEQPSIRMMILRG
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EGKSFSAGADLNWMERASTHSREDNETDAMRLADMLRRLYEMPQMTLALVQGAAMGGGAG
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
LVAACDVAIAKAGTQFRFSEVRLGLTPATISPFVIQAIGPRWAKALFVTADGFDAAYAEK
HHHHHHHHHHHCCCCEEHHHEEECCCCCCCCHHHHHHCCCHHHEEEEEEECCCCHHHHHH
IGLVHYVAETDEQMGELQEHIARLVFSAAPGAIADAKQLVADVTGMEIDNSLMHTTAKRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AARRVSDEGKEGIAAFLEKRTPSWKA
HHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDAVYDLIRFEATEEGLAVVTLHRPDVHNAFNAELIAELTDVFTMISEQPSIRMMILRG
CCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
EGKSFSAGADLNWMERASTHSREDNETDAMRLADMLRRLYEMPQMTLALVQGAAMGGGAG
CCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
LVAACDVAIAKAGTQFRFSEVRLGLTPATISPFVIQAIGPRWAKALFVTADGFDAAYAEK
HHHHHHHHHHHCCCCEEHHHEEECCCCCCCCHHHHHHCCCHHHEEEEEEECCCCHHHHHH
IGLVHYVAETDEQMGELQEHIARLVFSAAPGAIADAKQLVADVTGMEIDNSLMHTTAKRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AARRVSDEGKEGIAAFLEKRTPSWKA
HHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA