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The map label for this gene is dapA

Identifier: 114798403

GI number: 114798403

Start: 2961828

End: 2962709

Strand: Reverse

Name: dapA

Synonym: HNE_2800

Alternate gene names: 114798403

Gene position: 2962709-2961828 (Counterclockwise)

Preceding gene: 114797304

Following gene: 114797634

Centisome position: 79.96

GC content: 65.08

Gene sequence:

>882_bases
ATGTTTCACGGCTCAATCCCAGCCCTTGTAACACCCTTCAAGAATGGCGCTGTCGACGAGGCGGCCTTTGCGGCGCTGGT
CGAGCGGCAGATTGCGGCTGGATCGGCCGCGCTGGTGCCGGTCGGCACGACGGGGGAAACCTCGACGCTGTCGACCGAGG
AGCACAAGCGCGTTGTCTCGCTTTGTGTAGAGGTGGCTGCAGGACGCGTGCCGGTGATCGCCGGGGCGGGATCGAATTCC
ACCGATGAAGCGATTGACCTGGTGGCCCATGCCAAGGCGGCAGGCGCAGACGCGGCGCTGGTGGTGAGCCCCTATTACAA
TAATCCCAATCAGGATGGCCTGTTCGAACACTTTCGCGCGATCAATGACGCCGTCGCCCTGCCCGTGGTGCTTTATAATG
TGCCCAGCCGCACGGTGGTGGACCTGAAACCGGAGACCGTGGCGCGCCTGGCGCGGCTGCCCAATATCGTCGGCATCAAG
GACGCGACCGGCGACATGGACCGGGTGAGCTATCATTCGGCGCTGATCGGGGACGAGGAACAGTTCGTGCAGCTTTCGGG
CGATGACCCTTCGGCGCTGGGTTTTCTGGCGATGGGCGGGGCGGGATGTATCTCGGTGACCGCGAATGTGGCGCCGGAGC
TCTGCGCGGCGATGCATCTGGCGTTTGAGGACGGCGATCTGGAAAGCGCGCGGGCAATCGAGCGGCGGCTGATTAACCTG
CACCGGGCGATGTTCTGCTCACCTTCGCCGGGGCCGGCGAAATATGCGCTCTCGCGGATGGGGCTGTGCGGGCCGGAGGT
TCGCCTGCCGCTGACGGCGCCAGATGCGGCCGCTGAAGCGATGATCGACGCGGCCATGGCGCTGGCCGGTCTGAACACCT
AG

Upstream 100 bases:

>100_bases
GTTCGGCTCACCCAAGTTTATTTCTGCGCCAAAGCGTGACAAGTCCAGAGACAGAGCCTAGCACGCAATCCCAACCAATC
CCTGAATACGGCACGTTAAA

Downstream 100 bases:

>100_bases
ATTGGGCCGATGGCTACGAAGAAGAACGAACAGATCAAAGGGCGCACCGACGGGCTGGTGGCGGAAAACCGCCGCTCCCG
GCGCGAGTATTCCGTGGAGG

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MFHGSIPALVTPFKNGAVDEAAFAALVERQIAAGSAALVPVGTTGETSTLSTEEHKRVVSLCVEVAAGRVPVIAGAGSNS
TDEAIDLVAHAKAAGADAALVVSPYYNNPNQDGLFEHFRAINDAVALPVVLYNVPSRTVVDLKPETVARLARLPNIVGIK
DATGDMDRVSYHSALIGDEEQFVQLSGDDPSALGFLAMGGAGCISVTANVAPELCAAMHLAFEDGDLESARAIERRLINL
HRAMFCSPSPGPAKYALSRMGLCGPEVRLPLTAPDAAAEAMIDAAMALAGLNT

Sequences:

>Translated_293_residues
MFHGSIPALVTPFKNGAVDEAAFAALVERQIAAGSAALVPVGTTGETSTLSTEEHKRVVSLCVEVAAGRVPVIAGAGSNS
TDEAIDLVAHAKAAGADAALVVSPYYNNPNQDGLFEHFRAINDAVALPVVLYNVPSRTVVDLKPETVARLARLPNIVGIK
DATGDMDRVSYHSALIGDEEQFVQLSGDDPSALGFLAMGGAGCISVTANVAPELCAAMHLAFEDGDLESARAIERRLINL
HRAMFCSPSPGPAKYALSRMGLCGPEVRLPLTAPDAAAEAMIDAAMALAGLNT
>Mature_293_residues
MFHGSIPALVTPFKNGAVDEAAFAALVERQIAAGSAALVPVGTTGETSTLSTEEHKRVVSLCVEVAAGRVPVIAGAGSNS
TDEAIDLVAHAKAAGADAALVVSPYYNNPNQDGLFEHFRAINDAVALPVVLYNVPSRTVVDLKPETVARLARLPNIVGIK
DATGDMDRVSYHSALIGDEEQFVQLSGDDPSALGFLAMGGAGCISVTANVAPELCAAMHLAFEDGDLESARAIERRLINL
HRAMFCSPSPGPAKYALSRMGLCGPEVRLPLTAPDAAAEAMIDAAMALAGLNT

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=287, Percent_Identity=30.3135888501742, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI13540533, Length=249, Percent_Identity=28.1124497991968, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1788823, Length=293, Percent_Identity=47.4402730375427, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI87082415, Length=269, Percent_Identity=36.4312267657993, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786463, Length=246, Percent_Identity=36.1788617886179, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789620, Length=237, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_HYPNA (Q0BYG4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761479.1
- ProteinModelPortal:   Q0BYG4
- SMR:   Q0BYG4
- STRING:   Q0BYG4
- GeneID:   4289744
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2800
- NMPDR:   fig|228405.5.peg.2685
- TIGR:   HNE_2800
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   ELLCYPM
- PhylomeDB:   Q0BYG4
- ProtClustDB:   PRK03170
- BioCyc:   HNEP81032:HNE_2800-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 30329; Mature: 30329

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 160-160 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHGSIPALVTPFKNGAVDEAAFAALVERQIAAGSAALVPVGTTGETSTLSTEEHKRVVS
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
LCVEVAAGRVPVIAGAGSNSTDEAIDLVAHAKAAGADAALVVSPYYNNPNQDGLFEHFRA
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
INDAVALPVVLYNVPSRTVVDLKPETVARLARLPNIVGIKDATGDMDRVSYHSALIGDEE
HHHHHHHEEEEEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCHH
QFVQLSGDDPSALGFLAMGGAGCISVTANVAPELCAAMHLAFEDGDLESARAIERRLINL
HEEEECCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HRAMFCSPSPGPAKYALSRMGLCGPEVRLPLTAPDAAAEAMIDAAMALAGLNT
HHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFHGSIPALVTPFKNGAVDEAAFAALVERQIAAGSAALVPVGTTGETSTLSTEEHKRVVS
CCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHH
LCVEVAAGRVPVIAGAGSNSTDEAIDLVAHAKAAGADAALVVSPYYNNPNQDGLFEHFRA
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHH
INDAVALPVVLYNVPSRTVVDLKPETVARLARLPNIVGIKDATGDMDRVSYHSALIGDEE
HHHHHHHEEEEEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCHH
QFVQLSGDDPSALGFLAMGGAGCISVTANVAPELCAAMHLAFEDGDLESARAIERRLINL
HEEEECCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
HRAMFCSPSPGPAKYALSRMGLCGPEVRLPLTAPDAAAEAMIDAAMALAGLNT
HHHHCCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA