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The map label for this gene is gatB [H]

Identifier: 114798220

GI number: 114798220

Start: 2391615

End: 2393123

Strand: Reverse

Name: gatB [H]

Synonym: HNE_2291

Alternate gene names: 114798220

Gene position: 2393123-2391615 (Counterclockwise)

Preceding gene: 114800360

Following gene: 114799980

Centisome position: 64.59

GC content: 60.97

Gene sequence:

>1509_bases
ATGACCGAGCGCACCCCCTTCCGCATCAAAGGCGAAACCGGCGACTGGGAAGTCGTCATGGGGCTCGAAATCCATGCGCA
GGTGGCCTCCAACGCCAAGCTGTTCTCCGGCGCGTCCACCACCTTTGGCGCAGACCCCAACTGCAACGTCTCGCTCGTGG
ACGCCGCGATGCCGGGCATGCTGCCGGTCATCAACAAATTCTGCGTGGAGCAGGCGATCCGCACCGGGCTTGGCCTGAAG
GCGCAGATCAATACCTACAGCCGTTTCGACCGGAAGAACTATTTCTATCCTGACCTGCCGCAGGGCTATCAGATCAGCCA
GTTTGCCCACCCTATCGTGGGCGAGGGCGAGATTGAGGTGGATGTCGAGGTGCCGGGCAAGGAGGATTATGCCTTCCCTT
GCCGTATCGAGCGCCTGCACCTGGAACAGGACGCGGGCAAGTCGATCCACGACATGGACCCCAACTCGACCTATGTGGAC
CTCAACCGCTCGGGCGTGGCGCTGATGGAGATCGTCTCCAAGCCCGACATCCGTTCGCCGGCGGAAGCGGCTGCGTATGT
CAAGAAGCTGCGCGCCATCGTGATCGCGCTGGGTACCTGTGACGGCGACATGGAGAAGGGCAACCTGCGCGCCGACGTGA
ACGTTTCGGTGTGCAAGCCGGGCCAGTATGAGAAGTTCCGCGAGACCGGCGATTTCAAACATCTGGGCACGCGCTGCGAG
CTCAAGAACATGAACTCCTACCGCTTCATCCAGCAGGCCATCGAATATGAGGCCCACCGCCAGATCGAGATCATCGAGGG
CGGTGGCAAGGTGGATCAGGAGACCCGGCTGTTCGATCCGGTGAAGGGCGAGACCCGCTCCATGCGCTCGAAGGAAGACG
CGCATGACTACCGCTATTTTCCGGACCCGGATCTTCTGCCTTTGGAAGTCGAGCAGGCCTGGATCGAAGAGATCCGTGCC
TCGCTGCCCGAGCTGCCAGACCAGAAGCGCGCGCGGTTCGAAAAGGATTACGGTCTCTCCCGCTATGACGCAGGCGTGCT
GACCTCTGACGCCGACAAGGCGGCCTTCTTTGAGGAAGCTGCCAAGGGCCGCGACGCGAAGCTCACCGCCAACTGGGTGA
CGCAGGAGCTGTTCGGCTATCTCAACAAGGAAGGCCTAGAGCTTTCAGAGAGCCCGGTTTCGGCCGAAGCGCTCGGTGGG
CTGATCGAGCTGATCTCGAACGACACGATCAGCGGCAAGATCGCCAAGGATGTCTTTGCCCGGATGATTGCGGGCGAGGG
CGAGGCGGCTGCCATCGTGGAGAAGCACGGGCTGAAACAGGTCACCGATACCGGCGCGATCGAGAAGGTCGTCGATGAAA
TCATCGCGGCCAATCCCGATCAGGTCGCCAAGGTGAAAGACAAGCCGCAGACGCTTGGCTGGTTTGTCGGTCAGGTGATG
AAAGCTTCGGGCGGCAAGGCCAATCCGAAGGCTGTGAACGATATTCTGAAGGCTAAGCTGGGCATTTGA

Upstream 100 bases:

>100_bases
AGATCTGATCCGCCCGGCGGCGCGCCAGTTCGCCGTCTCGACAAGCCTGCCCGGCTGCCCTTAGAAGGGCGCCACAAACC
TTATAATCCTGAGAGAAGCG

Downstream 100 bases:

>100_bases
CCCGTCTCCCCCCTTAAGCCCCCTCTCCCCGGAGGGGCGAGGGGGCTTAAGACAGCCTCAAACCGCTGCCCGTATCTTTG
CTGCAATCGGCTCCAGCTCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTERTPFRIKGETGDWEVVMGLEIHAQVASNAKLFSGASTTFGADPNCNVSLVDAAMPGMLPVINKFCVEQAIRTGLGLK
AQINTYSRFDRKNYFYPDLPQGYQISQFAHPIVGEGEIEVDVEVPGKEDYAFPCRIERLHLEQDAGKSIHDMDPNSTYVD
LNRSGVALMEIVSKPDIRSPAEAAAYVKKLRAIVIALGTCDGDMEKGNLRADVNVSVCKPGQYEKFRETGDFKHLGTRCE
LKNMNSYRFIQQAIEYEAHRQIEIIEGGGKVDQETRLFDPVKGETRSMRSKEDAHDYRYFPDPDLLPLEVEQAWIEEIRA
SLPELPDQKRARFEKDYGLSRYDAGVLTSDADKAAFFEEAAKGRDAKLTANWVTQELFGYLNKEGLELSESPVSAEALGG
LIELISNDTISGKIAKDVFARMIAGEGEAAAIVEKHGLKQVTDTGAIEKVVDEIIAANPDQVAKVKDKPQTLGWFVGQVM
KASGGKANPKAVNDILKAKLGI

Sequences:

>Translated_502_residues
MTERTPFRIKGETGDWEVVMGLEIHAQVASNAKLFSGASTTFGADPNCNVSLVDAAMPGMLPVINKFCVEQAIRTGLGLK
AQINTYSRFDRKNYFYPDLPQGYQISQFAHPIVGEGEIEVDVEVPGKEDYAFPCRIERLHLEQDAGKSIHDMDPNSTYVD
LNRSGVALMEIVSKPDIRSPAEAAAYVKKLRAIVIALGTCDGDMEKGNLRADVNVSVCKPGQYEKFRETGDFKHLGTRCE
LKNMNSYRFIQQAIEYEAHRQIEIIEGGGKVDQETRLFDPVKGETRSMRSKEDAHDYRYFPDPDLLPLEVEQAWIEEIRA
SLPELPDQKRARFEKDYGLSRYDAGVLTSDADKAAFFEEAAKGRDAKLTANWVTQELFGYLNKEGLELSESPVSAEALGG
LIELISNDTISGKIAKDVFARMIAGEGEAAAIVEKHGLKQVTDTGAIEKVVDEIIAANPDQVAKVKDKPQTLGWFVGQVM
KASGGKANPKAVNDILKAKLGI
>Mature_501_residues
TERTPFRIKGETGDWEVVMGLEIHAQVASNAKLFSGASTTFGADPNCNVSLVDAAMPGMLPVINKFCVEQAIRTGLGLKA
QINTYSRFDRKNYFYPDLPQGYQISQFAHPIVGEGEIEVDVEVPGKEDYAFPCRIERLHLEQDAGKSIHDMDPNSTYVDL
NRSGVALMEIVSKPDIRSPAEAAAYVKKLRAIVIALGTCDGDMEKGNLRADVNVSVCKPGQYEKFRETGDFKHLGTRCEL
KNMNSYRFIQQAIEYEAHRQIEIIEGGGKVDQETRLFDPVKGETRSMRSKEDAHDYRYFPDPDLLPLEVEQAWIEEIRAS
LPELPDQKRARFEKDYGLSRYDAGVLTSDADKAAFFEEAAKGRDAKLTANWVTQELFGYLNKEGLELSESPVSAEALGGL
IELISNDTISGKIAKDVFARMIAGEGEAAAIVEKHGLKQVTDTGAIEKVVDEIIAANPDQVAKVKDKPQTLGWFVGQVMK
ASGGKANPKAVNDILKAKLGI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=512, Percent_Identity=41.40625, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI115534094, Length=363, Percent_Identity=38.0165289256198, Blast_Score=227, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=543, Percent_Identity=30.939226519337, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=36.5853658536585, Blast_Score=311, Evalue=8e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 55414; Mature: 55283

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERTPFRIKGETGDWEVVMGLEIHAQVASNAKLFSGASTTFGADPNCNVSLVDAAMPGM
CCCCCCEEEECCCCCEEEEEEEEEEEEHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCH
LPVINKFCVEQAIRTGLGLKAQINTYSRFDRKNYFYPDLPQGYQISQFAHPIVGEGEIEV
HHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEE
DVEVPGKEDYAFPCRIERLHLEQDAGKSIHDMDPNSTYVDLNRSGVALMEIVSKPDIRSP
EEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCH
AEAAAYVKKLRAIVIALGTCDGDMEKGNLRADVNVSVCKPGQYEKFRETGDFKHLGTRCE
HHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCCEEE
LKNMNSYRFIQQAIEYEAHRQIEIIEGGGKVDQETRLFDPVKGETRSMRSKEDAHDYRYF
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCCHHHHCCCCHHHCCCCC
PDPDLLPLEVEQAWIEEIRASLPELPDQKRARFEKDYGLSRYDAGVLTSDADKAAFFEEA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCEECCCHHHHHHHHH
AKGRDAKLTANWVTQELFGYLNKEGLELSESPVSAEALGGLIELISNDTISGKIAKDVFA
HCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RMIAGEGEAAAIVEKHGLKQVTDTGAIEKVVDEIIAANPDQVAKVKDKPQTLGWFVGQVM
HHHCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHH
KASGGKANPKAVNDILKAKLGI
HHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TERTPFRIKGETGDWEVVMGLEIHAQVASNAKLFSGASTTFGADPNCNVSLVDAAMPGM
CCCCCEEEECCCCCEEEEEEEEEEEEHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCH
LPVINKFCVEQAIRTGLGLKAQINTYSRFDRKNYFYPDLPQGYQISQFAHPIVGEGEIEV
HHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEE
DVEVPGKEDYAFPCRIERLHLEQDAGKSIHDMDPNSTYVDLNRSGVALMEIVSKPDIRSP
EEECCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCH
AEAAAYVKKLRAIVIALGTCDGDMEKGNLRADVNVSVCKPGQYEKFRETGDFKHLGTRCE
HHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCCEEE
LKNMNSYRFIQQAIEYEAHRQIEIIEGGGKVDQETRLFDPVKGETRSMRSKEDAHDYRYF
ECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCCHHHHCCCCHHHCCCCC
PDPDLLPLEVEQAWIEEIRASLPELPDQKRARFEKDYGLSRYDAGVLTSDADKAAFFEEA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHCCCCEECCCHHHHHHHHH
AKGRDAKLTANWVTQELFGYLNKEGLELSESPVSAEALGGLIELISNDTISGKIAKDVFA
HCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RMIAGEGEAAAIVEKHGLKQVTDTGAIEKVVDEIIAANPDQVAKVKDKPQTLGWFVGQVM
HHHCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHH
KASGGKANPKAVNDILKAKLGI
HHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]