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The map label for this gene is nth [H]

Identifier: 114798206

GI number: 114798206

Start: 2116762

End: 2117526

Strand: Reverse

Name: nth [H]

Synonym: HNE_2040

Alternate gene names: 114798206

Gene position: 2117526-2116762 (Counterclockwise)

Preceding gene: 114798961

Following gene: 114797353

Centisome position: 57.15

GC content: 63.92

Gene sequence:

>765_bases
ATGCCTCAGCCTATGCCTGATGCTTCCAAACCTGCCCGCAAGCCCGCCAAAGCCGTCCCGCGCTCAAAGTTCACCCGCGC
GAGGGCGGCGGAGCTCTTTGCCGCGCTTGCCGCAGACCGCCCCGATCCAGCGACCGAGTTGGAGTTCGTAAACCCCTTCA
CCCTGCTCGTCGCCGTCGCCCTGTCCGCGCAGGCCACCGATGTCGGTGTGAATAAAGCCACGCGGAAGCTGTTCGCTGTC
GCCGACACTCCCGAAAAAATGCTCACCCTTGGTGAGGAAGGGGTGGCCAGCCACATCCGGACCATCGGCCTCTGGCGCAA
CAAGGCAAAGAACGTCATCGCGCTCAGCCGCAGGATCATCGAGGAATACGGCGGCGAGGTGCCGCGCACGCGGGATGAAC
TCACCACCCTGCCGGGCGTGGGCCGCAAGACCGCAAATGTCGTCATGAACGAAGCCTTCGGTGAGCCCACGATCGCCGTC
GACACTCACATCTTCCGTGTTTCCAACAGAACCGGGCTCGCGCCCGGCAAGACGCCCGACCATGTCGAGACGGGCCTCGA
GCGCATTACCCCGCCGGAATTCAAGAAGGGCGCCCATCACTGGCTGATCCTGCACGGGCGGTATGTGTGCAAGGCGCGTA
CCCCCGAATGCTGGCACTGTGCGATCAAGGATCTCTGTGATTTCAAGCCGAAAACGCCCGACCCGGCGGCCCAGGCCGCG
TTAGGGCCGAAAAAACCCCGCAAATCCGCTGGCGCCGCAAAGTGA

Upstream 100 bases:

>100_bases
ACCGGTTGGGGGTCAGCACGGCGTCCATGTAGACGGTTTCATGCGGGGGTGTCATGAACGGAAGAAATAGCACGCCCTTG
CGCTGCGGGAAGGGCGCTCT

Downstream 100 bases:

>100_bases
ATTGACCTTGCGGGCTGCCTCGGCAAACCTTTTTGAGGTGTGATGTGAAAAGGACTGGAACCATGCGCAGCAAGCTGTTC
GGGGCAGGGGCTGCCAAAGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPQPMPDASKPARKPAKAVPRSKFTRARAAELFAALAADRPDPATELEFVNPFTLLVAVALSAQATDVGVNKATRKLFAV
ADTPEKMLTLGEEGVASHIRTIGLWRNKAKNVIALSRRIIEEYGGEVPRTRDELTTLPGVGRKTANVVMNEAFGEPTIAV
DTHIFRVSNRTGLAPGKTPDHVETGLERITPPEFKKGAHHWLILHGRYVCKARTPECWHCAIKDLCDFKPKTPDPAAQAA
LGPKKPRKSAGAAK

Sequences:

>Translated_254_residues
MPQPMPDASKPARKPAKAVPRSKFTRARAAELFAALAADRPDPATELEFVNPFTLLVAVALSAQATDVGVNKATRKLFAV
ADTPEKMLTLGEEGVASHIRTIGLWRNKAKNVIALSRRIIEEYGGEVPRTRDELTTLPGVGRKTANVVMNEAFGEPTIAV
DTHIFRVSNRTGLAPGKTPDHVETGLERITPPEFKKGAHHWLILHGRYVCKARTPECWHCAIKDLCDFKPKTPDPAAQAA
LGPKKPRKSAGAAK
>Mature_253_residues
PQPMPDASKPARKPAKAVPRSKFTRARAAELFAALAADRPDPATELEFVNPFTLLVAVALSAQATDVGVNKATRKLFAVA
DTPEKMLTLGEEGVASHIRTIGLWRNKAKNVIALSRRIIEEYGGEVPRTRDELTTLPGVGRKTANVVMNEAFGEPTIAVD
THIFRVSNRTGLAPGKTPDHVETGLERITPPEFKKGAHHWLILHGRYVCKARTPECWHCAIKDLCDFKPKTPDPAAQAAL
GPKKPRKSAGAAK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=31.6062176165803, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=58.3732057416268, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=32.0224719101124, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27603; Mature: 27472

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPMPDASKPARKPAKAVPRSKFTRARAAELFAALAADRPDPATELEFVNPFTLLVAVA
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHH
LSAQATDVGVNKATRKLFAVADTPEKMLTLGEEGVASHIRTIGLWRNKAKNVIALSRRII
HHCCHHHCCHHHHHHHHEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYGGEVPRTRDELTTLPGVGRKTANVVMNEAFGEPTIAVDTHIFRVSNRTGLAPGKTPD
HHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCCCCCHH
HVETGLERITPPEFKKGAHHWLILHGRYVCKARTPECWHCAIKDLCDFKPKTPDPAAQAA
HHHHHHHHCCCHHHHCCCCEEEEEECEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHH
LGPKKPRKSAGAAK
CCCCCCCCCCCCCC
>Mature Secondary Structure 
PQPMPDASKPARKPAKAVPRSKFTRARAAELFAALAADRPDPATELEFVNPFTLLVAVA
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHH
LSAQATDVGVNKATRKLFAVADTPEKMLTLGEEGVASHIRTIGLWRNKAKNVIALSRRII
HHCCHHHCCHHHHHHHHEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEYGGEVPRTRDELTTLPGVGRKTANVVMNEAFGEPTIAVDTHIFRVSNRTGLAPGKTPD
HHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCCCCCHH
HVETGLERITPPEFKKGAHHWLILHGRYVCKARTPECWHCAIKDLCDFKPKTPDPAAQAA
HHHHHHHHCCCHHHHCCCCEEEEEECEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHH
LGPKKPRKSAGAAK
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]