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The map label for this gene is ycjG [H]

Identifier: 114798174

GI number: 114798174

Start: 1644949

End: 1646019

Strand: Reverse

Name: ycjG [H]

Synonym: HNE_1610

Alternate gene names: 114798174

Gene position: 1646019-1644949 (Counterclockwise)

Preceding gene: 114798992

Following gene: 114797864

Centisome position: 44.43

GC content: 60.32

Gene sequence:

>1071_bases
GTGCTGCCCGCGATCCTCCTTCCACGGAACCAGAATATGAATAGAAGTTTCTGCGCTTACAAAACATCATGGCCCCTGCG
CACGCCTATTCGCATTACGGGGCATGTCTTTGAAAAGATCGACATGGTGGTTGTTGAAATCGGGCAGGATGGTCACACCG
GCCGGGGCGAGGGCTGCGGCGTCTATTACACCAATGACTTCGCCGATGGCATGCTGGAACAGATCGAGCTCGTGCGCGGA
GACATCCTTGCGGGAATGACACGGGAAGAATTGCAGAGCGCCCTGCCCCCTGGCGGCGCCCGCAATGCGCTCGATTGCGC
TTTATGGGATCTGGACGCGCGCCTCGATGGACGGGGCGTCTGGGACATGCTTTCGATTTCCCCCGATCCGGTCCGTACTG
CATTCACGATCAGCATCAACAGCGCCGAGGAAATGGCCCGGCAGGCCGCCAAAGCTGCTGACTACTCCCTGATCAAGATC
AAGCTTGATGCCGAAACGCCCGTGGAGCGGGTCGCGGCCATTCGCGCGGCACGTCCCGATGCAACTTTGATCATCGACGC
CAATGAGGGCTTCAGTTTCGCGCAGCTGCGCAGAACGGCAGATGCGCTGGCAGATCTGGGCGTTGCCATGATCGAACAGC
CCCTGCCACGGGGCGGCGACAGCGAACTTGAAGGTTATAAATCTCCTGTCCCTCTGTGTGCAGACGAAAGCTGCCTTCAC
CGCGGCGAGCTTTCCCAGGCGCTTGAGCGGTATGATATGATCAATATCAAGCTGGACAAGACAGGCGGCCTCACCGAGGC
ACTTGCCCTGGCGGCCGAAGCCCGCAAGGCCGGCAAGGCACTTATGGTAGGCAATATGGTGGGAACATCCTTGTCTATGG
CTCCGGCATTTGCGATCGCGCAGCTCTGCGAGTTTGCAGACCTTGACGGTCCGCTTGCCCTCAGATCGGATCTGGTCAAC
GGACTTGTTTACCGCAAGGGTATGGTTGGACCGCCTGCGCCGGAGTTCTGGGGCGGCCCGCTCGCGCCCAGGCGCCTTGC
CGCCAGCCACCCGCCGGAGACGACACATTGA

Upstream 100 bases:

>100_bases
GGGACTTTGTCCGGCATCTGAACCCTCCGGATGCCGGACATTTTTTCATAGAGCATGTCCAGCTGACATGTTTGTCCGCC
CTGTCCGCGAACCACTTTGT

Downstream 100 bases:

>100_bases
TATCACCGGACAATGTATTCGGACTTCTGGCTGTTTTCCTGGTCCTGTCAGCCATAGCGGTGGCCCTGGAGAAAACACCG
GTCGGCCGGCGTGTTTCCGG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: (R)-mandelate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLPAILLPRNQNMNRSFCAYKTSWPLRTPIRITGHVFEKIDMVVVEIGQDGHTGRGEGCGVYYTNDFADGMLEQIELVRG
DILAGMTREELQSALPPGGARNALDCALWDLDARLDGRGVWDMLSISPDPVRTAFTISINSAEEMARQAAKAADYSLIKI
KLDAETPVERVAAIRAARPDATLIIDANEGFSFAQLRRTADALADLGVAMIEQPLPRGGDSELEGYKSPVPLCADESCLH
RGELSQALERYDMINIKLDKTGGLTEALALAAEARKAGKALMVGNMVGTSLSMAPAFAIAQLCEFADLDGPLALRSDLVN
GLVYRKGMVGPPAPEFWGGPLAPRRLAASHPPETTH

Sequences:

>Translated_356_residues
MLPAILLPRNQNMNRSFCAYKTSWPLRTPIRITGHVFEKIDMVVVEIGQDGHTGRGEGCGVYYTNDFADGMLEQIELVRG
DILAGMTREELQSALPPGGARNALDCALWDLDARLDGRGVWDMLSISPDPVRTAFTISINSAEEMARQAAKAADYSLIKI
KLDAETPVERVAAIRAARPDATLIIDANEGFSFAQLRRTADALADLGVAMIEQPLPRGGDSELEGYKSPVPLCADESCLH
RGELSQALERYDMINIKLDKTGGLTEALALAAEARKAGKALMVGNMVGTSLSMAPAFAIAQLCEFADLDGPLALRSDLVN
GLVYRKGMVGPPAPEFWGGPLAPRRLAASHPPETTH
>Mature_356_residues
MLPAILLPRNQNMNRSFCAYKTSWPLRTPIRITGHVFEKIDMVVVEIGQDGHTGRGEGCGVYYTNDFADGMLEQIELVRG
DILAGMTREELQSALPPGGARNALDCALWDLDARLDGRGVWDMLSISPDPVRTAFTISINSAEEMARQAAKAADYSLIKI
KLDAETPVERVAAIRAARPDATLIIDANEGFSFAQLRRTADALADLGVAMIEQPLPRGGDSELEGYKSPVPLCADESCLH
RGELSQALERYDMINIKLDKTGGLTEALALAAEARKAGKALMVGNMVGTSLSMAPAFAIAQLCEFADLDGPLALRSDLVN
GLVYRKGMVGPPAPEFWGGPLAPRRLAASHPPETTH

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=317, Percent_Identity=46.3722397476341, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.1.2.2

Molecular weight: Translated: 38383; Mature: 38383

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00501 SPASE_I_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAILLPRNQNMNRSFCAYKTSWPLRTPIRITGHVFEKIDMVVVEIGQDGHTGRGEGCG
CCCCEEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHEEEEEEECCCCCCCCCCCEE
VYYTNDFADGMLEQIELVRGDILAGMTREELQSALPPGGARNALDCALWDLDARLDGRGV
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
WDMLSISPDPVRTAFTISINSAEEMARQAAKAADYSLIKIKLDAETPVERVAAIRAARPD
CCEECCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCC
ATLIIDANEGFSFAQLRRTADALADLGVAMIEQPLPRGGDSELEGYKSPVPLCADESCLH
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHH
RGELSQALERYDMINIKLDKTGGLTEALALAAEARKAGKALMVGNMVGTSLSMAPAFAIA
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHH
QLCEFADLDGPLALRSDLVNGLVYRKGMVGPPAPEFWGGPLAPRRLAASHPPETTH
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MLPAILLPRNQNMNRSFCAYKTSWPLRTPIRITGHVFEKIDMVVVEIGQDGHTGRGEGCG
CCCCEEECCCCCCCCCEEEEECCCCCCCCCEEHHHHHHHHEEEEEEECCCCCCCCCCCEE
VYYTNDFADGMLEQIELVRGDILAGMTREELQSALPPGGARNALDCALWDLDARLDGRGV
EEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
WDMLSISPDPVRTAFTISINSAEEMARQAAKAADYSLIKIKLDAETPVERVAAIRAARPD
CCEECCCCCCCCEEEEEEECCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCC
ATLIIDANEGFSFAQLRRTADALADLGVAMIEQPLPRGGDSELEGYKSPVPLCADESCLH
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHH
RGELSQALERYDMINIKLDKTGGLTEALALAAEARKAGKALMVGNMVGTSLSMAPAFAIA
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCHHCCCHHHHHHHHHH
QLCEFADLDGPLALRSDLVNGLVYRKGMVGPPAPEFWGGPLAPRRLAASHPPETTH
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-mandelate

Specific reaction: (S)-mandelate = (R)-mandelate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]