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The map label for this gene is metG

Identifier: 114798101

GI number: 114798101

Start: 1888945

End: 1890684

Strand: Direct

Name: metG

Synonym: HNE_1831

Alternate gene names: 114798101

Gene position: 1888945-1890684 (Clockwise)

Preceding gene: 114800488

Following gene: 114797573

Centisome position: 50.98

GC content: 59.08

Gene sequence:

>1740_bases
ATGACCCAGCAACGGCGCATCCTCATCACCTCGGCCCTGCCCTACATCAACGGCGTGAAGCATCTCGGCAATCTCGCCGG
CTCAATGCTGCCAGCCGATGTTTACGCCCGTGTGATGCGCCTTCAGGGCCATGACGTAACCTACATCTGCGCCACCGATG
AACACGGCACCCCGGCCGAACTGGCCGCTCAGGCGACGGGCCAAAGCGTACAGGCCTATTGCGACGAACAGTATGAGGTA
CAGCGCAAAGCTGGCGAAGGCTTTAACCTGTCTTTCGATTGGTTTGGCCGCACATCCCGCCCGGCCAACCACACGCTGAC
CCAGCACCTCGCCCAGCAGCTCGAGAAGAACGGCCTGATCGAAGTGCGCACCTCAAAACAGGTTTACGCTGTCGATGACG
GCCGGTTCCTGCCGGACCGTTATGTTGAGGGCACTTGCCCCCATTGTGGCTATGAAAAAGCCCGCGGCGACCAGTGCGAC
AATTGCGGGCGGCTGCTTGATCCCGTTGACCTCATCAATCCCTATTCGGCCGTCTCGGGCAGCCGTAACATTGAAATCCG
CGACACGGACCACCTCTACCTACTGCAAACAGGTATGCAGGACCGTATCCGCGACTGGGTAAACGCCAAGGGCAAGAACT
GGCCGTCTCTGGCGGTCTCCATTGCCAACAAATGGCTGGATGAGGGCCTTATCGCCCGGTCCATCTCCCGCGACCTCAGC
TGGGGCGTCAAAGTTACGGACGCTGACGGCAATCCCCGGCCGGGCTTCGAGAACAAGGTCTTTTATGTCTGGTTCGATGC
GCCGATCGGTTACATCTCCGCCACGCAGGAATGGGCTGAAGCGACCGGCAATGACTGGGAAAAACTCTGGCTGACCGACA
AGGGCGCCGATCAGACCGAATACGTCCAGTTCATGGGCAAGGACAACGTCGCCTTCCACACCGTCAGCTTCCCCGTGACG
CTCCTCGGCTCAGGCGAACCCTGGAAAACCGTGGACAAGCTGAAAGCCTTCAACTGGGTTACCTGGTATGGCGGCAAGTT
CTCCACCAGCCAGAAGCGCGGCGTGTTCATGGACCAGGCCTTAAGCCTTCTGCCGTCTGACTATTGGCGCTGGTATCTCA
TCTCCAATGCGCCGGAAGGCTCTGACGCGGCGTTCACCTGGGAAGGTTTCCAGGCAGCCGTGAACTCCGATCTCGCCAAT
GTTCTCGGCAACTTCATCAACCGGATCACCAAATACTGTGCTTCTAAATTCGAAGGCAAAATTCCCGAAACCGGCACATC
CGGCGACGCGGAAGCCTGGATGGCCAACGAACTCGCAGAGCGCCTGCCCCGCCTCATTGAATTCTATGAAGCCATGGAGT
TCCGCAAGGCTGCTGCCGAAACCCGCGCCATCTGGGCGGCGGGCAACGAATACCTCACTAAGGCCGAGCCTTGGGTGAAG
TACAAAAATGATGTTGACGGCGCCGCTGTCGGCGTTCGCGCGGGCATCAATCTTGCGGCCATCTTCGGCATCATCGCCCA
GCCAATCATTCCGGATGCGGCAAAGAAGATTCTCGATGCCCTGGGCATCCCGGAAGAAAATCGCAAGATGCCGGCAGGCT
CAAAGCCCGAAGACTTCGCCGCCCTGCTCGATGCAATCCCGCGCGGCCATGCAATCTCGCCACCAGACGTTCTCTTCCAG
AAGATCGAGGATAGCCAGGTTGAAGAATGGACCGCCCAGTTCGGCGGTGGTAAGGAGTAA

Upstream 100 bases:

>100_bases
TTGCGCAGGTTCACATATTGGGGCCGCGCCTAGAGCTTTCCTTTAGCGGTGAGGGCGTTAAAAGGCGCGTTCTCCAACAG
AAAGGCTCTGAAAAGGCCCC

Downstream 100 bases:

>100_bases
GCCCATGCCCAAAGCGCGATCAGGAGGCTGCCAGTGCGGGCGCATCCGGTTTCGCGCCGAAAGCCTGAGGGACAACTCGC
ATGTCTGTCATTGCCGGATG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTQQRRILITSALPYINGVKHLGNLAGSMLPADVYARVMRLQGHDVTYICATDEHGTPAELAAQATGQSVQAYCDEQYEV
QRKAGEGFNLSFDWFGRTSRPANHTLTQHLAQQLEKNGLIEVRTSKQVYAVDDGRFLPDRYVEGTCPHCGYEKARGDQCD
NCGRLLDPVDLINPYSAVSGSRNIEIRDTDHLYLLQTGMQDRIRDWVNAKGKNWPSLAVSIANKWLDEGLIARSISRDLS
WGVKVTDADGNPRPGFENKVFYVWFDAPIGYISATQEWAEATGNDWEKLWLTDKGADQTEYVQFMGKDNVAFHTVSFPVT
LLGSGEPWKTVDKLKAFNWVTWYGGKFSTSQKRGVFMDQALSLLPSDYWRWYLISNAPEGSDAAFTWEGFQAAVNSDLAN
VLGNFINRITKYCASKFEGKIPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVK
YKNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFAALLDAIPRGHAISPPDVLFQ
KIEDSQVEEWTAQFGGGKE

Sequences:

>Translated_579_residues
MTQQRRILITSALPYINGVKHLGNLAGSMLPADVYARVMRLQGHDVTYICATDEHGTPAELAAQATGQSVQAYCDEQYEV
QRKAGEGFNLSFDWFGRTSRPANHTLTQHLAQQLEKNGLIEVRTSKQVYAVDDGRFLPDRYVEGTCPHCGYEKARGDQCD
NCGRLLDPVDLINPYSAVSGSRNIEIRDTDHLYLLQTGMQDRIRDWVNAKGKNWPSLAVSIANKWLDEGLIARSISRDLS
WGVKVTDADGNPRPGFENKVFYVWFDAPIGYISATQEWAEATGNDWEKLWLTDKGADQTEYVQFMGKDNVAFHTVSFPVT
LLGSGEPWKTVDKLKAFNWVTWYGGKFSTSQKRGVFMDQALSLLPSDYWRWYLISNAPEGSDAAFTWEGFQAAVNSDLAN
VLGNFINRITKYCASKFEGKIPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVK
YKNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFAALLDAIPRGHAISPPDVLFQ
KIEDSQVEEWTAQFGGGKE
>Mature_578_residues
TQQRRILITSALPYINGVKHLGNLAGSMLPADVYARVMRLQGHDVTYICATDEHGTPAELAAQATGQSVQAYCDEQYEVQ
RKAGEGFNLSFDWFGRTSRPANHTLTQHLAQQLEKNGLIEVRTSKQVYAVDDGRFLPDRYVEGTCPHCGYEKARGDQCDN
CGRLLDPVDLINPYSAVSGSRNIEIRDTDHLYLLQTGMQDRIRDWVNAKGKNWPSLAVSIANKWLDEGLIARSISRDLSW
GVKVTDADGNPRPGFENKVFYVWFDAPIGYISATQEWAEATGNDWEKLWLTDKGADQTEYVQFMGKDNVAFHTVSFPVTL
LGSGEPWKTVDKLKAFNWVTWYGGKFSTSQKRGVFMDQALSLLPSDYWRWYLISNAPEGSDAAFTWEGFQAAVNSDLANV
LGNFINRITKYCASKFEGKIPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVKY
KNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFAALLDAIPRGHAISPPDVLFQK
IEDSQVEEWTAQFGGGKE

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=581, Percent_Identity=39.4148020654045, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI24308436, Length=549, Percent_Identity=23.8615664845173, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI1788432, Length=581, Percent_Identity=31.6695352839931, Blast_Score=262, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17540794, Length=584, Percent_Identity=36.6438356164384, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645096, Length=553, Percent_Identity=23.869801084991, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=436, Percent_Identity=22.2477064220184, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321704, Length=578, Percent_Identity=35.8131487889273, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655311, Length=581, Percent_Identity=37.1772805507745, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI21358483, Length=539, Percent_Identity=26.3450834879406, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_HYPNA (Q0C161)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760532.1
- STRING:   Q0C161
- GeneID:   4289596
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1831
- NMPDR:   fig|228405.5.peg.1762
- TIGR:   HNE_1831
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   GSREPWK
- PhylomeDB:   Q0C161
- ProtClustDB:   PRK12268
- BioCyc:   HNEP81032:HNE_1831-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 64436; Mature: 64305

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS00300 SRP54

Important sites: BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQRRILITSALPYINGVKHLGNLAGSMLPADVYARVMRLQGHDVTYICATDEHGTPAE
CCCCCEEEEEHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LAAQATGQSVQAYCDEQYEVQRKAGEGFNLSFDWFGRTSRPANHTLTQHLAQQLEKNGLI
HHHHHCCCHHHHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCE
EVRTSKQVYAVDDGRFLPDRYVEGTCPHCGYEKARGDQCDNCGRLLDPVDLINPYSAVSG
EEECCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCCHHHCCCHHHCCC
SRNIEIRDTDHLYLLQTGMQDRIRDWVNAKGKNWPSLAVSIANKWLDEGLIARSISRDLS
CCCEEEECCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
WGVKVTDADGNPRPGFENKVFYVWFDAPIGYISATQEWAEATGNDWEKLWLTDKGADQTE
CCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
YVQFMGKDNVAFHTVSFPVTLLGSGEPWKTVDKLKAFNWVTWYGGKFSTSQKRGVFMDQA
HHHHHCCCCEEEEEEECCEEEECCCCCCHHHHHHHHCCEEEEECCEECCCCCCCCHHHHH
LSLLPSDYWRWYLISNAPEGSDAAFTWEGFQAAVNSDLANVLGNFINRITKYCASKFEGK
HHHCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCCCCCCCE
YKNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFA
ECCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
ALLDAIPRGHAISPPDVLFQKIEDSQVEEWTAQFGGGKE
HHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQQRRILITSALPYINGVKHLGNLAGSMLPADVYARVMRLQGHDVTYICATDEHGTPAE
CCCCEEEEEHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LAAQATGQSVQAYCDEQYEVQRKAGEGFNLSFDWFGRTSRPANHTLTQHLAQQLEKNGLI
HHHHHCCCHHHHHHCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCE
EVRTSKQVYAVDDGRFLPDRYVEGTCPHCGYEKARGDQCDNCGRLLDPVDLINPYSAVSG
EEECCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCCHHHCCCHHHCCC
SRNIEIRDTDHLYLLQTGMQDRIRDWVNAKGKNWPSLAVSIANKWLDEGLIARSISRDLS
CCCEEEECCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
WGVKVTDADGNPRPGFENKVFYVWFDAPIGYISATQEWAEATGNDWEKLWLTDKGADQTE
CCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
YVQFMGKDNVAFHTVSFPVTLLGSGEPWKTVDKLKAFNWVTWYGGKFSTSQKRGVFMDQA
HHHHHCCCCEEEEEEECCEEEECCCCCCHHHHHHHHCCEEEEECCEECCCCCCCCHHHHH
LSLLPSDYWRWYLISNAPEGSDAAFTWEGFQAAVNSDLANVLGNFINRITKYCASKFEGK
HHHCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPETGTSGDAEAWMANELAERLPRLIEFYEAMEFRKAAAETRAIWAAGNEYLTKAEPWVK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHCCCCCCCE
YKNDVDGAAVGVRAGINLAAIFGIIAQPIIPDAAKKILDALGIPEENRKMPAGSKPEDFA
ECCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
ALLDAIPRGHAISPPDVLFQKIEDSQVEEWTAQFGGGKE
HHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA