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The map label for this gene is rihA [H]

Identifier: 114798081

GI number: 114798081

Start: 193085

End: 194017

Strand: Reverse

Name: rihA [H]

Synonym: HNE_0208

Alternate gene names: 114798081

Gene position: 194017-193085 (Counterclockwise)

Preceding gene: 114797114

Following gene: 114799341

Centisome position: 5.24

GC content: 66.67

Gene sequence:

>933_bases
GTGACCGCCCCCCTGCCCCTCATCATCGATTGCGATCCCGGCATCGACGACGCCGTCATGCTGATGATGGCGCTCGGCTC
GCCCCGCTTTGATGTCCGCGCAATTACCACCGTGGCGGGCAACGTCCCCCTGCGCCTCACCAGCCGCAATGCCCGCATGA
TGGGCGAGCTGATGGGCCGGCCCGACATCCCCGTCTTTGCCGGCTGCCCACGCCCGATGCTGCGCCCGCCCGTCACGGCC
GAGGACTTCCACGGCGAAAGCGGCATCTACGGCATCGACGTGTTCGAACCAAAAGCCCCGCTCCAGCCATCCCACGCCGT
CGACGCCCTCATCCGTCTGCTGAAAGCCGCCCCGCATAAGGGCATGACCCTCGTCGTCACCGGCCCGATGACCAATCTCG
CCTGCGCCCTCGTCATGGCGCCGGAGATCGCCGCTCATATCCGCGAGATCGTCATCATGGGCGGCGCCGATACCGAAGGC
GGCAACATCACCCCCTTCGCCGAGTTCAACATCTTCGCCGATCCCCACGCGGCCGCCATCGTGCTCGAAACCGGCCTCCC
CGCCACCATCCTCAGCCTTGACGCCACCCACCAGGTCCGCGCGACGCAGGCCCGCATCGATCGCCTCGCCCAGATCCCCG
GCCCCCGCGCCGCGATGATGGCCTCGCTGCTGACGGCCGCCAACAACCTCGAAACCCGCGCCCGCCCCGGCACCAAAGTC
CCGATGCATGATCCCTCCACCATCGCCTGGCTGCTCGCCCCGGAAATGTTCGAGACGGTCAACGCCCGCGTCACGGTCAC
CACAAGACCCGGCAAGGAATTTGGCCGCACGAAAGTCCGCCCCATGAAGACGGGCCCCCATCGCTGGGTCACAAAAGCGA
ATGCCGATGCCTTCTTTGCACGGATCGAAGACATATTGAGGATCGCGCCATGA

Upstream 100 bases:

>100_bases
CGGCAGACATGGCGATGGAAGCGCTCGGCGTCACGCTGGACAGCCCGCCGCTGGCGCCGCATGTTCAGGCCCTCCTTGAA
GATCTGAGATCCGCCGCCAC

Downstream 100 bases:

>100_bases
TCACCGTCGTCGGCTCGGTCAATCTCGATTTTGTCGCCAGCGGAAAATCCCTCCCCCATGCGGGGGAAACCGTCACCGGC
GCCCGCCTCGCGCGCCATCC

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTAPLPLIIDCDPGIDDAVMLMMALGSPRFDVRAITTVAGNVPLRLTSRNARMMGELMGRPDIPVFAGCPRPMLRPPVTA
EDFHGESGIYGIDVFEPKAPLQPSHAVDALIRLLKAAPHKGMTLVVTGPMTNLACALVMAPEIAAHIREIVIMGGADTEG
GNITPFAEFNIFADPHAAAIVLETGLPATILSLDATHQVRATQARIDRLAQIPGPRAAMMASLLTAANNLETRARPGTKV
PMHDPSTIAWLLAPEMFETVNARVTVTTRPGKEFGRTKVRPMKTGPHRWVTKANADAFFARIEDILRIAP

Sequences:

>Translated_310_residues
MTAPLPLIIDCDPGIDDAVMLMMALGSPRFDVRAITTVAGNVPLRLTSRNARMMGELMGRPDIPVFAGCPRPMLRPPVTA
EDFHGESGIYGIDVFEPKAPLQPSHAVDALIRLLKAAPHKGMTLVVTGPMTNLACALVMAPEIAAHIREIVIMGGADTEG
GNITPFAEFNIFADPHAAAIVLETGLPATILSLDATHQVRATQARIDRLAQIPGPRAAMMASLLTAANNLETRARPGTKV
PMHDPSTIAWLLAPEMFETVNARVTVTTRPGKEFGRTKVRPMKTGPHRWVTKANADAFFARIEDILRIAP
>Mature_309_residues
TAPLPLIIDCDPGIDDAVMLMMALGSPRFDVRAITTVAGNVPLRLTSRNARMMGELMGRPDIPVFAGCPRPMLRPPVTAE
DFHGESGIYGIDVFEPKAPLQPSHAVDALIRLLKAAPHKGMTLVVTGPMTNLACALVMAPEIAAHIREIVIMGGADTEGG
NITPFAEFNIFADPHAAAIVLETGLPATILSLDATHQVRATQARIDRLAQIPGPRAAMMASLLTAANNLETRARPGTKVP
MHDPSTIAWLLAPEMFETVNARVTVTTRPGKEFGRTKVRPMKTGPHRWVTKANADAFFARIEDILRIAP

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=276, Percent_Identity=41.6666666666667, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=37.3376623376623, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1786213, Length=279, Percent_Identity=38.7096774193548, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32565474, Length=191, Percent_Identity=37.696335078534, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17565698, Length=258, Percent_Identity=31.0077519379845, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=211, Percent_Identity=33.175355450237, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583915, Length=234, Percent_Identity=34.1880341880342, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24641839, Length=199, Percent_Identity=37.1859296482412, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24641837, Length=208, Percent_Identity=35.5769230769231, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33336; Mature: 33204

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLPLIIDCDPGIDDAVMLMMALGSPRFDVRAITTVAGNVPLRLTSRNARMMGELMGR
CCCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHCC
PDIPVFAGCPRPMLRPPVTAEDFHGESGIYGIDVFEPKAPLQPSHAVDALIRLLKAAPHK
CCCCEEECCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
GMTLVVTGPMTNLACALVMAPEIAAHIREIVIMGGADTEGGNITPFAEFNIFADPHAAAI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEEEECCCCEEE
VLETGLPATILSLDATHQVRATQARIDRLAQIPGPRAAMMASLLTAANNLETRARPGTKV
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PMHDPSTIAWLLAPEMFETVNARVTVTTRPGKEFGRTKVRPMKTGPHRWVTKANADAFFA
CCCCCCCEEEEECHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCEEEECCHHHHHH
RIEDILRIAP
HHHHHHHCCC
>Mature Secondary Structure 
TAPLPLIIDCDPGIDDAVMLMMALGSPRFDVRAITTVAGNVPLRLTSRNARMMGELMGR
CCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCHHHHHHHHCC
PDIPVFAGCPRPMLRPPVTAEDFHGESGIYGIDVFEPKAPLQPSHAVDALIRLLKAAPHK
CCCCEEECCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCC
GMTLVVTGPMTNLACALVMAPEIAAHIREIVIMGGADTEGGNITPFAEFNIFADPHAAAI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEEEEEECCCCEEE
VLETGLPATILSLDATHQVRATQARIDRLAQIPGPRAAMMASLLTAANNLETRARPGTKV
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
PMHDPSTIAWLLAPEMFETVNARVTVTTRPGKEFGRTKVRPMKTGPHRWVTKANADAFFA
CCCCCCCEEEEECHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCEEEECCHHHHHH
RIEDILRIAP
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA