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The map label for this gene is xth [H]

Identifier: 114798068

GI number: 114798068

Start: 2003988

End: 2004764

Strand: Direct

Name: xth [H]

Synonym: HNE_1935

Alternate gene names: 114798068

Gene position: 2003988-2004764 (Clockwise)

Preceding gene: 114797679

Following gene: 114799389

Centisome position: 54.09

GC content: 60.23

Gene sequence:

>777_bases
ATGCGTATAGCGACCTGGAATGTCAATTCGATGAAGGCCCGCCTGCAGACTGTTCTGCAGGTGCTGACCGATATTAATGC
AGATGTTGTCTGCCTGCAGGAACTGAAGTGCGAGACAGACGCCTTTCCCTATCTCGAAATAGAGGAGCTGGGCTGGAACT
GCGCGGTTCACGGCCAGAAAAGCTATAACGGCGTCGCCCTGCTCTCGAAATTCCCCTTGCAGGATGTCACGCGGGAGATG
TCCACCCTGAAGGACGATCAGGCCCGCTACATCGAGGCCACCGTGCTGACCGACAAGCCGGTCCGCGTCGGCGGTCTCTA
TCTGCCCAACGGCAACCCGGCGCCCGGCCCGAAATATGACTACAAGCTCGAATGGGTCGCCGCGCTGGAAGACCACGCCC
GCAGTCTGCTCAAGGCCGAGGAGCCTTTCGTACTTTGCGGCGATTACAACGCCATCCCTGCGCCGGAAGATTGCTGGGAC
GATACGGTCTGGAACACCGACGCCCTCGGCCTGCCCGAAACACGCGCCGCCTGGCGCCAGCTCAAGCATCTCGGCCTCAC
CGATGCCTTCGAGGTCTGTGATGGGCGCGCCCATCAATACACGTTCTGGGATTATCAGGGCGGCGCCTTCCAGAAAGATC
ATGGCATCCGCATCGACCATATCCTCGCCTGCCCCCGCATGACCGACCGGCTCACCGCCATCGAAATCTACCGCAAGGCC
CGCGCGATGGACAAACCTTCCGACCATGTGCCGATGATCGCGATATTTGAGGACTGA

Upstream 100 bases:

>100_bases
ATCCAGCTTGGCGCCATGAACGCGACTCCTCAGGTTTGGGTGAATATAGAGCCCGACTGCTGCTGGAACAATGGGACGAA
GCTGCTTAAGAGGTTACCGC

Downstream 100 bases:

>100_bases
CCCCGTTTAGCTTCCGGAGCCCCGCATGCCCCCGCATGTTTTGCCAATTGTCCTTCTGTCGATCTCCAACCTCTTCATGA
CCATGGCCTGGTATGGTCAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRIATWNVNSMKARLQTVLQVLTDINADVVCLQELKCETDAFPYLEIEELGWNCAVHGQKSYNGVALLSKFPLQDVTREM
STLKDDQARYIEATVLTDKPVRVGGLYLPNGNPAPGPKYDYKLEWVAALEDHARSLLKAEEPFVLCGDYNAIPAPEDCWD
DTVWNTDALGLPETRAAWRQLKHLGLTDAFEVCDGRAHQYTFWDYQGGAFQKDHGIRIDHILACPRMTDRLTAIEIYRKA
RAMDKPSDHVPMIAIFED

Sequences:

>Translated_258_residues
MRIATWNVNSMKARLQTVLQVLTDINADVVCLQELKCETDAFPYLEIEELGWNCAVHGQKSYNGVALLSKFPLQDVTREM
STLKDDQARYIEATVLTDKPVRVGGLYLPNGNPAPGPKYDYKLEWVAALEDHARSLLKAEEPFVLCGDYNAIPAPEDCWD
DTVWNTDALGLPETRAAWRQLKHLGLTDAFEVCDGRAHQYTFWDYQGGAFQKDHGIRIDHILACPRMTDRLTAIEIYRKA
RAMDKPSDHVPMIAIFED
>Mature_258_residues
MRIATWNVNSMKARLQTVLQVLTDINADVVCLQELKCETDAFPYLEIEELGWNCAVHGQKSYNGVALLSKFPLQDVTREM
STLKDDQARYIEATVLTDKPVRVGGLYLPNGNPAPGPKYDYKLEWVAALEDHARSLLKAEEPFVLCGDYNAIPAPEDCWD
DTVWNTDALGLPETRAAWRQLKHLGLTDAFEVCDGRAHQYTFWDYQGGAFQKDHGIRIDHILACPRMTDRLTAIEIYRKA
RAMDKPSDHVPMIAIFED

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=28.6259541984733, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=28.6259541984733, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=28.6259541984733, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=29.7397769516729, Blast_Score=117, Evalue=6e-28,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29258; Mature: 29258

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSMKARLQTVLQVLTDINADVVCLQELKCETDAFPYLEIEELGWNCAVHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCEEHHHCCCEEEECCCC
SYNGVALLSKFPLQDVTREMSTLKDDQARYIEATVLTDKPVRVGGLYLPNGNPAPGPKYD
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECEEECCCCCCCCCCCCC
YKLEWVAALEDHARSLLKAEEPFVLCGDYNAIPAPEDCWDDTVWNTDALGLPETRAAWRQ
EEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
LKHLGLTDAFEVCDGRAHQYTFWDYQGGAFQKDHGIRIDHILACPRMTDRLTAIEIYRKA
HHHCCCCHHHHHHCCCCCEEEEEECCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHH
RAMDKPSDHVPMIAIFED
HHCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRIATWNVNSMKARLQTVLQVLTDINADVVCLQELKCETDAFPYLEIEELGWNCAVHGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCCEEHHHCCCEEEECCCC
SYNGVALLSKFPLQDVTREMSTLKDDQARYIEATVLTDKPVRVGGLYLPNGNPAPGPKYD
CCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECEEECCCCCCCCCCCCC
YKLEWVAALEDHARSLLKAEEPFVLCGDYNAIPAPEDCWDDTVWNTDALGLPETRAAWRQ
EEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
LKHLGLTDAFEVCDGRAHQYTFWDYQGGAFQKDHGIRIDHILACPRMTDRLTAIEIYRKA
HHHCCCCHHHHHHCCCCCEEEEEECCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHH
RAMDKPSDHVPMIAIFED
HHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]