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The map label for this gene is argH

Identifier: 114798021

GI number: 114798021

Start: 3601452

End: 3602849

Strand: Reverse

Name: argH

Synonym: HNE_3465

Alternate gene names: 114798021

Gene position: 3602849-3601452 (Counterclockwise)

Preceding gene: 114799034

Following gene: 114800174

Centisome position: 97.24

GC content: 62.52

Gene sequence:

>1398_bases
ATGGCAGATGACAAAGGCCAGATGATGTGGGGCGGGCGTTTTGCCGCGACACCTTCAGCCATCATGGAAGAGATCAATGC
CTCGCTGGATATTGACCGGCGGATGGCGGAAGAAGATGTTGCCGGTAGCCGCGCCCATGCCGACATGCTGGCCGAAATGG
GCATTCTGAGCGTCGCCGATAATGAGGCGATCCAGGGTGGCCTCGACGCCGTGATCGAGGAGATGCGGGCAGGGACATTC
CCTTTCCGCCGAGAGCTGGAAGACATTCACATGAATGTCGAAGCCCGCCTCAAGGAGCTGATCGGCGAGCCGGCCGGGCG
CCTGCATACGGCGCGGTCGCGCAATGATCAGGTGATCACAGACTTCCGTCTCTGGACGCGGCGGGCCTTGGACGAGACCG
GCCAACTGGTGGCGGGCCTTCAGGCAATGCTGGTGCGCCGGGCTGACGAGAATACGTCCACCGTGATGCCGGGCTTTACC
CATTTGCAGACCGCGCAGCCGGTGACGCTCGGGCATCACTTGCTGGCCTATGTCGAGATGCTGGAACGCGACCGCTCCCG
CCTGCTCGATTGCGCGGTGCGGCTGAATGAGTGTCCGCTGGGCGCTGCCGCGCTGGCCGGCACTGGCTTTCCGATCGACC
GCGACATGACGGCTGAAGCGCTCGGCTTTGCCCGGCCGATGGCAAACTCGCTGGACGCGGTTTCTGCGCGGGATTTCGCG
CTGGAAGCGCTCTCCAGCCTTTCGATTGCCGCCACGCACCTTTCGCGTCTGGCTGAAGAGATCGTGCTGTGGACGAGCCC
GCAATTCGGCTTTGCGCGGCTGAGCGATCAATGGTCGACGGGGTCTTCGATCATGCCTCAGAAACGCAATCCGGATGCGG
CTGAGCTGATCCGGGCGAAGGCATCACTGATCACGGGGCATTTCTCGGCATTGCAGGGGGCCATCAAGGCGCTGCCGCTG
GCCTATGCGAAGGATCTGCAGGACGACAAACGCCTCACTTTTGACGCCTTCGACACGTTTAATCTGTGTGTCCGGGCAAT
GACCGGCATGATCGAAACCATCAGCTTCAAGCCCGACGCCATGCGGGCAGCCGCGGCCAAAGGGTTCTCTACGGCGACGG
ACCTGGCGGACTGGCTGGTTCGGGAACTGGGAATGCCGTTCCGGGATGCCCACCATGTTACGGGCCGCATTGTTGCGCGG
GCCGAGGCCAAGGGGGTTGATCTGGCTGATCTGCCCCTGAAGGAGATGCAGGCCGTGCACAGCGCCATCACACAGGAGGT
ATATGGCGTGCTCAGCGTTGAAGCGTCAGCTGCATCGCGTACCTCATATGGCGCCACGAGCCCTGTTCGCGTGGCCGAAC
AGGTGGCACAGTGGAAACAGAGACTTCGAGTGGAGTAG

Upstream 100 bases:

>100_bases
GACAATCGCACCAAGGCCGATCAAGAAGAGGCCGAGATAGGCGTAGCGGGTCATGGGACGGCCCTTCACTGACGTCGGTT
ACGAATAAGAGGATATGGCA

Downstream 100 bases:

>100_bases
TCCCATGAAACGCACCCTCGCCCTTGCGGCGGTTATTCTTTTCACCACCACACTGTCTGCCTGCGGCCTGCAAGGTCCGC
TGCAGCGCCCCGGTCCCCTG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MADDKGQMMWGGRFAATPSAIMEEINASLDIDRRMAEEDVAGSRAHADMLAEMGILSVADNEAIQGGLDAVIEEMRAGTF
PFRRELEDIHMNVEARLKELIGEPAGRLHTARSRNDQVITDFRLWTRRALDETGQLVAGLQAMLVRRADENTSTVMPGFT
HLQTAQPVTLGHHLLAYVEMLERDRSRLLDCAVRLNECPLGAAALAGTGFPIDRDMTAEALGFARPMANSLDAVSARDFA
LEALSSLSIAATHLSRLAEEIVLWTSPQFGFARLSDQWSTGSSIMPQKRNPDAAELIRAKASLITGHFSALQGAIKALPL
AYAKDLQDDKRLTFDAFDTFNLCVRAMTGMIETISFKPDAMRAAAAKGFSTATDLADWLVRELGMPFRDAHHVTGRIVAR
AEAKGVDLADLPLKEMQAVHSAITQEVYGVLSVEASAASRTSYGATSPVRVAEQVAQWKQRLRVE

Sequences:

>Translated_465_residues
MADDKGQMMWGGRFAATPSAIMEEINASLDIDRRMAEEDVAGSRAHADMLAEMGILSVADNEAIQGGLDAVIEEMRAGTF
PFRRELEDIHMNVEARLKELIGEPAGRLHTARSRNDQVITDFRLWTRRALDETGQLVAGLQAMLVRRADENTSTVMPGFT
HLQTAQPVTLGHHLLAYVEMLERDRSRLLDCAVRLNECPLGAAALAGTGFPIDRDMTAEALGFARPMANSLDAVSARDFA
LEALSSLSIAATHLSRLAEEIVLWTSPQFGFARLSDQWSTGSSIMPQKRNPDAAELIRAKASLITGHFSALQGAIKALPL
AYAKDLQDDKRLTFDAFDTFNLCVRAMTGMIETISFKPDAMRAAAAKGFSTATDLADWLVRELGMPFRDAHHVTGRIVAR
AEAKGVDLADLPLKEMQAVHSAITQEVYGVLSVEASAASRTSYGATSPVRVAEQVAQWKQRLRVE
>Mature_464_residues
ADDKGQMMWGGRFAATPSAIMEEINASLDIDRRMAEEDVAGSRAHADMLAEMGILSVADNEAIQGGLDAVIEEMRAGTFP
FRRELEDIHMNVEARLKELIGEPAGRLHTARSRNDQVITDFRLWTRRALDETGQLVAGLQAMLVRRADENTSTVMPGFTH
LQTAQPVTLGHHLLAYVEMLERDRSRLLDCAVRLNECPLGAAALAGTGFPIDRDMTAEALGFARPMANSLDAVSARDFAL
EALSSLSIAATHLSRLAEEIVLWTSPQFGFARLSDQWSTGSSIMPQKRNPDAAELIRAKASLITGHFSALQGAIKALPLA
YAKDLQDDKRLTFDAFDTFNLCVRAMTGMIETISFKPDAMRAAAAKGFSTATDLADWLVRELGMPFRDAHHVTGRIVARA
EAKGVDLADLPLKEMQAVHSAITQEVYGVLSVEASAASRTSYGATSPVRVAEQVAQWKQRLRVE

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=457, Percent_Identity=43.3260393873085, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303542, Length=457, Percent_Identity=43.3260393873085, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303547, Length=457, Percent_Identity=41.3566739606127, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI68303549, Length=457, Percent_Identity=40.9190371991247, Blast_Score=328, Evalue=8e-90,
Organism=Escherichia coli, GI1790398, Length=455, Percent_Identity=43.7362637362637, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787896, Length=336, Percent_Identity=26.4880952380952, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=281, Percent_Identity=25.6227758007117, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32565146, Length=217, Percent_Identity=28.110599078341, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321806, Length=463, Percent_Identity=39.524838012959, Blast_Score=334, Evalue=1e-92,
Organism=Drosophila melanogaster, GI221473854, Length=455, Percent_Identity=39.3406593406593, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI78706858, Length=455, Percent_Identity=39.3406593406593, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI78710009, Length=222, Percent_Identity=28.3783783783784, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583245, Length=224, Percent_Identity=28.125, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640179, Length=224, Percent_Identity=27.2321428571429, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640177, Length=224, Percent_Identity=27.2321428571429, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_HYPNA (Q0BWK6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762137.1
- ProteinModelPortal:   Q0BWK6
- SMR:   Q0BWK6
- STRING:   Q0BWK6
- GeneID:   4290077
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3465
- NMPDR:   fig|228405.5.peg.3318
- TIGR:   HNE_3465
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- PhylomeDB:   Q0BWK6
- BioCyc:   HNEP81032:HNE_3465-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 50791; Mature: 50660

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDKGQMMWGGRFAATPSAIMEEINASLDIDRRMAEEDVAGSRAHADMLAEMGILSVAD
CCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHEECC
NEAIQGGLDAVIEEMRAGTFPFRRELEDIHMNVEARLKELIGEPAGRLHTARSRNDQVIT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCCCEEH
DFRLWTRRALDETGQLVAGLQAMLVRRADENTSTVMPGFTHLQTAQPVTLGHHLLAYVEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHH
LERDRSRLLDCAVRLNECPLGAAALAGTGFPIDRDMTAEALGFARPMANSLDAVSARDFA
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEALSSLSIAATHLSRLAEEIVLWTSPQFGFARLSDQWSTGSSIMPQKRNPDAAELIRAK
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
ASLITGHFSALQGAIKALPLAYAKDLQDDKRLTFDAFDTFNLCVRAMTGMIETISFKPDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHH
MRAAAAKGFSTATDLADWLVRELGMPFRDAHHVTGRIVARAEAKGVDLADLPLKEMQAVH
HHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHCCCCCCCCCCHHHHHHHH
SAITQEVYGVLSVEASAASRTSYGATSPVRVAEQVAQWKQRLRVE
HHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADDKGQMMWGGRFAATPSAIMEEINASLDIDRRMAEEDVAGSRAHADMLAEMGILSVAD
CCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHEECC
NEAIQGGLDAVIEEMRAGTFPFRRELEDIHMNVEARLKELIGEPAGRLHTARSRNDQVIT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCCCEEH
DFRLWTRRALDETGQLVAGLQAMLVRRADENTSTVMPGFTHLQTAQPVTLGHHLLAYVEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHH
LERDRSRLLDCAVRLNECPLGAAALAGTGFPIDRDMTAEALGFARPMANSLDAVSARDFA
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEALSSLSIAATHLSRLAEEIVLWTSPQFGFARLSDQWSTGSSIMPQKRNPDAAELIRAK
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
ASLITGHFSALQGAIKALPLAYAKDLQDDKRLTFDAFDTFNLCVRAMTGMIETISFKPDA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHH
MRAAAAKGFSTATDLADWLVRELGMPFRDAHHVTGRIVARAEAKGVDLADLPLKEMQAVH
HHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEHHCCCCCCCCCCHHHHHHHH
SAITQEVYGVLSVEASAASRTSYGATSPVRVAEQVAQWKQRLRVE
HHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA