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The map label for this gene is metZ [H]

Identifier: 114797978

GI number: 114797978

Start: 2832562

End: 2833761

Strand: Reverse

Name: metZ [H]

Synonym: HNE_2672

Alternate gene names: 114797978

Gene position: 2833761-2832562 (Counterclockwise)

Preceding gene: 114800013

Following gene: 114799279

Centisome position: 76.48

GC content: 64.5

Gene sequence:

>1200_bases
ATGGCGGATGCACCCGGCGGCGACAAGAAGGGCTGGAAGCCTGCGACCCAGGCGGTACGCGGCGGCCTGATGCGGTCCCA
GCATGGGGAGATTTCCGAGGCGCTGTATCTGACCTCCGGCTACGCTTACGACTCGGCCGAGCAGGCGATGCGCCGGATGG
CGGGCGAGGAAGAAGGCTTCGTCTATTCCCGCTATGGCAGCCCGACCAATGAGATGCTGCAACAGCGCCTCGCGCTGATT
GAAGGCGCCGAAGCGTGCCGGGTGACGGGCTCTGGCATGGGCGCGATTTCGTCGGCCATCCTGGCGCCGCTTAAAGCGGG
CGACCGGGTGGTGGCGGCGACCGCGCTGTTTGGCTCGTGCCGCTGGATCATTGCCAACCAGATGCCGAAGTTTGGCATCG
AGGCAGTGTTCGTGGACGGGGCCGATCTTGATGCTTGGAAGCGCGAGATCGACAAGGGCTGCCAGCTGGTGCTGATCGAA
AGCCCGGCCAATCCGTTGCTCGACGGCGTGGACATCGAAGCGGTCGCCAGGCTCGCCAAGGCGGCGGGCGCGCTGCTGGT
GGTGGACAATGTGTTTGCCACGCCGGTGCTTCAGCGGCCGCTGGAAATGGGCGCCGATGTGATCGCCTATTCGGCCACCA
AACATATGGACGGGCAGGGCCGCGTTCTGCTGGGCGCGATCCTGACGGACGCCAAGCGGATGAGTGATGTGTATGATCCG
TGGCTGCGCCATATGGGGCCGGCGGCCTCGCCGTTTAACGCCTGGGTAGTGCTGAAGGGCCTTGAGACGATGCAGCTGCG
CGTGGAAGCGCAGAGCCGCACGGCGGCGCGGCTGGCGGATGTTCTGGCCGATCATCCGGCGGTCAATGCCGTGCGCTATC
CCCACCGCAAGGATCACCCGCATTATGAGGTGCACAAGCGCCAGATGAAATCGGGCGGCACGCTGCTCGCGCTGTCGCTC
AAGGGCGGGCAGGACGCGGCGTTCCGCTTCCTCAACGGGCTGCAGCTGGTCGACATCTGCAACAACCTTGGCGATACGAA
ATCGCTGGCCTGTCATCCCTCCACCACGACGCACCGCGCGTTGAGTGATGAGGATCAGGCGGCGATGGGGCTTGACCGCA
GCTGGGTCCGGCTCTCTGTTGGTCTTGAAGACGCAGATGATCTGGAAGCTGATCTTCTCGCTTCGCTTAACAGCTTGTGA

Upstream 100 bases:

>100_bases
AATCTCGAAATCGGGGATAACCCCGCTCATATCAGCCATCACTTGCACCCTGTGGGTCTCAGGCATAAGACCCGCTGCTG
GAAACACAAGGATCAGAGCC

Downstream 100 bases:

>100_bases
ACTTATGCCGGGGAAGGCTTGGCCCATGGCAAGACGCCCCGCTCCTCCACAGTATGAGGCCATCACCGATGGCGTGCGCA
TTCGTGTGCGCCCCAAATTC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MADAPGGDKKGWKPATQAVRGGLMRSQHGEISEALYLTSGYAYDSAEQAMRRMAGEEEGFVYSRYGSPTNEMLQQRLALI
EGAEACRVTGSGMGAISSAILAPLKAGDRVVAATALFGSCRWIIANQMPKFGIEAVFVDGADLDAWKREIDKGCQLVLIE
SPANPLLDGVDIEAVARLAKAAGALLVVDNVFATPVLQRPLEMGADVIAYSATKHMDGQGRVLLGAILTDAKRMSDVYDP
WLRHMGPAASPFNAWVVLKGLETMQLRVEAQSRTAARLADVLADHPAVNAVRYPHRKDHPHYEVHKRQMKSGGTLLALSL
KGGQDAAFRFLNGLQLVDICNNLGDTKSLACHPSTTTHRALSDEDQAAMGLDRSWVRLSVGLEDADDLEADLLASLNSL

Sequences:

>Translated_399_residues
MADAPGGDKKGWKPATQAVRGGLMRSQHGEISEALYLTSGYAYDSAEQAMRRMAGEEEGFVYSRYGSPTNEMLQQRLALI
EGAEACRVTGSGMGAISSAILAPLKAGDRVVAATALFGSCRWIIANQMPKFGIEAVFVDGADLDAWKREIDKGCQLVLIE
SPANPLLDGVDIEAVARLAKAAGALLVVDNVFATPVLQRPLEMGADVIAYSATKHMDGQGRVLLGAILTDAKRMSDVYDP
WLRHMGPAASPFNAWVVLKGLETMQLRVEAQSRTAARLADVLADHPAVNAVRYPHRKDHPHYEVHKRQMKSGGTLLALSL
KGGQDAAFRFLNGLQLVDICNNLGDTKSLACHPSTTTHRALSDEDQAAMGLDRSWVRLSVGLEDADDLEADLLASLNSL
>Mature_398_residues
ADAPGGDKKGWKPATQAVRGGLMRSQHGEISEALYLTSGYAYDSAEQAMRRMAGEEEGFVYSRYGSPTNEMLQQRLALIE
GAEACRVTGSGMGAISSAILAPLKAGDRVVAATALFGSCRWIIANQMPKFGIEAVFVDGADLDAWKREIDKGCQLVLIES
PANPLLDGVDIEAVARLAKAAGALLVVDNVFATPVLQRPLEMGADVIAYSATKHMDGQGRVLLGAILTDAKRMSDVYDPW
LRHMGPAASPFNAWVVLKGLETMQLRVEAQSRTAARLADVLADHPAVNAVRYPHRKDHPHYEVHKRQMKSGGTLLALSLK
GGQDAAFRFLNGLQLVDICNNLGDTKSLACHPSTTTHRALSDEDQAAMGLDRSWVRLSVGLEDADDLEADLLASLNSL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=329, Percent_Identity=33.1306990881459, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI299473759, Length=329, Percent_Identity=30.6990881458967, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI25453487, Length=328, Percent_Identity=28.3536585365854, Blast_Score=146, Evalue=4e-35,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=33.8582677165354, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=25.8883248730964, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=362, Percent_Identity=35.3591160220994, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=35.5421686746988, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI71980708, Length=380, Percent_Identity=29.7368421052632, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=33.5038363171356, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=411, Percent_Identity=29.683698296837, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=25.3694581280788, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=346, Percent_Identity=28.3236994219653, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321170, Length=286, Percent_Identity=26.5734265734266, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=36.046511627907, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42934; Mature: 42803

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAPGGDKKGWKPATQAVRGGLMRSQHGEISEALYLTSGYAYDSAEQAMRRMAGEEEGF
CCCCCCCCCCCCCHHHHHHHCCHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCCCCE
VYSRYGSPTNEMLQQRLALIEGAEACRVTGSGMGAISSAILAPLKAGDRVVAATALFGSC
EEEECCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCH
RWIIANQMPKFGIEAVFVDGADLDAWKREIDKGCQLVLIESPANPLLDGVDIEAVARLAK
HEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
AAGALLVVDNVFATPVLQRPLEMGADVIAYSATKHMDGQGRVLLGAILTDAKRMSDVYDP
HCCCEEEECCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
WLRHMGPAASPFNAWVVLKGLETMQLRVEAQSRTAARLADVLADHPAVNAVRYPHRKDHP
HHHHCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
HYEVHKRQMKSGGTLLALSLKGGQDAAFRFLNGLQLVDICNNLGDTKSLACHPSTTTHRA
CHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCHHHH
LSDEDQAAMGLDRSWVRLSVGLEDADDLEADLLASLNSL
CCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADAPGGDKKGWKPATQAVRGGLMRSQHGEISEALYLTSGYAYDSAEQAMRRMAGEEEGF
CCCCCCCCCCCCHHHHHHHCCHHHCCCCCHHHEEEEECCCCCCHHHHHHHHHCCCCCCE
VYSRYGSPTNEMLQQRLALIEGAEACRVTGSGMGAISSAILAPLKAGDRVVAATALFGSC
EEEECCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHCH
RWIIANQMPKFGIEAVFVDGADLDAWKREIDKGCQLVLIESPANPLLDGVDIEAVARLAK
HEEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
AAGALLVVDNVFATPVLQRPLEMGADVIAYSATKHMDGQGRVLLGAILTDAKRMSDVYDP
HCCCEEEECCHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHHH
WLRHMGPAASPFNAWVVLKGLETMQLRVEAQSRTAARLADVLADHPAVNAVRYPHRKDHP
HHHHCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
HYEVHKRQMKSGGTLLALSLKGGQDAAFRFLNGLQLVDICNNLGDTKSLACHPSTTTHRA
CHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCCHHHH
LSDEDQAAMGLDRSWVRLSVGLEDADDLEADLLASLNSL
CCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]