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The map label for this gene is narH [H]

Identifier: 114797890

GI number: 114797890

Start: 1591335

End: 1592864

Strand: Direct

Name: narH [H]

Synonym: HNE_1561

Alternate gene names: 114797890

Gene position: 1591335-1592864 (Clockwise)

Preceding gene: 114798640

Following gene: 114800087

Centisome position: 42.95

GC content: 57.71

Gene sequence:

>1530_bases
ATGAAAATCCGTGCACAAATCGGTATGGTCCTGAACCTCGACAAATGTATTGGTTGCCATACCTGCTCAGTGACCTGCAA
GAATGTCTGGACCAGCCGGGAGGGCGTTGAATACGCGTGGTTCAACAATGTCGAAACCAAGCCGGGCATCGGGTATCCGA
AGGATTGGGAAAACCAGAAGCGCTGGAATGGTGGCTGGCGGCGCAAGAAGAATGGCCGCATAGAACCCAGAATGGGCGCC
AAATGGCGCATTCTGGCGAAGATCTTCGCCAATCCGGACCTGCCCGAGATCGACGACTATTATGAGCCGTTCGATTTCGA
CTATGCCCACCTTCAGACCGCGCCGGAGATGAAAGCGTTTCCGACCGCGCGGCCACGTTCAAAGATTACCGGCGAGCGGA
TGGAAAAGATCGAATGGGGTCCGAACTGGGAGGAAATCCTTGGCGGGGAATTCGAGCACCGTAGCGCAGACTATAATTTC
GAAGGCGTCGAGAAGGCGATTTATGGCGCCTTTGAAGAAACCTTCATGATGTACCTGCCGCGCCTGTGCGAGCATTGCCT
CAACCCGACTTGCGTTGCGGCGTGCCCGTCAGGCGCGATCTATAAGCGCGAGGAAGACGGCATCGTCCTGATTGATCAGG
ACAAGTGCCGCGGCTGGCGGATGTGCGTCTCGGCCTGCCCGTACAAGAAAGTCTATTACAATTGGGAGAGCGGGAAGTCG
GAAAAATGCACCTTCTGCTATCCGCGTATGGAAGTCGGCCAGCCGACGGTCTGCTCTGAAACCTGTGTGGGTCGCATTCG
CTATCTTGGCGTCCTCCTCTATGACGCCGACCGGATAGAAGCAGCCGCCAGCACCCCCAATGAGCAGGATCTCTATCAGG
CGCAGCTTGACGTGTTTCTTGACCCGAATGACCCGAAAGTGATTGAGCAAGCCCGCGCTGACGGTGTACCAGAAGCCTGG
ATCAAGGCGGCTCAGGCGAGCCCGGTCTACAAGATGGCCATGGAATGGAAGGTCGCCTTTCCGCTGCACCCGGAATACCG
GACCCTCCCCATGGTCTGGTATGTGCCACCGCTCTCTCCGATCCAGTCGGCAGTCGCAGCCGGGGCCCTCGAGACCGATG
CGGAAATGCCGGACGTCGCCTCGATGCGCATTCCGGTGCGCTACCTTGCCAATCTGCTGACCGCCGGCAAGGAGGAGCCC
GTTGTTGTGGCGCTCGAACGGATGATGGCAATGCGCAAGTATATGCGCGCCAAGACCGTGGAGGGCGTGATCGACCATCC
GACGGCGGAACGCGTCGGTCTGACAGCCCGTCAGATTGACGAGATGTATCGCTACATGGCGATCGCCAATTATGAGGACC
GCTTCGTTATTCCGACGACACACCGCGAAACAAGCGAAGATGCTTACGGCCTTCGGGGGGATTGTGGCTTTACATTCGGC
AATGATTGCTCAGGCGGGCGCACCGAACTCGGTCTGTTCGGTAAGGACAAGCGTCCGCGTGCCAAAACCAAAACACCTAT
GGAGATGTGA

Upstream 100 bases:

>100_bases
ACGGCACGGTCGGATCGAACCGGGACGAGTTCATCATTCTGAGGAAGATGAACAAGGTCGACTGGCTCGATACCCCCGCC
GCTCAAAAGGAGGGCGTACA

Downstream 100 bases:

>100_bases
ACGATGGCCATGACCTATCGGGCGCTTTCGCGCCTATTGTCGTATCCCGAACCGCAACTTCAGACCGAAGCGAGCCTTTG
TGTGGAAATTGTCCGGAAAG

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKDWENQKRWNGGWRRKKNGRIEPRMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYAHLQTAPEMKAFPTARPRSKITGERMEKIEWGPNWEEILGGEFEHRSADYNF
EGVEKAIYGAFEETFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKVYYNWESGKS
EKCTFCYPRMEVGQPTVCSETCVGRIRYLGVLLYDADRIEAAASTPNEQDLYQAQLDVFLDPNDPKVIEQARADGVPEAW
IKAAQASPVYKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAVAAGALETDAEMPDVASMRIPVRYLANLLTAGKEEP
VVVALERMMAMRKYMRAKTVEGVIDHPTAERVGLTARQIDEMYRYMAIANYEDRFVIPTTHRETSEDAYGLRGDCGFTFG
NDCSGGRTELGLFGKDKRPRAKTKTPMEM

Sequences:

>Translated_509_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKDWENQKRWNGGWRRKKNGRIEPRMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYAHLQTAPEMKAFPTARPRSKITGERMEKIEWGPNWEEILGGEFEHRSADYNF
EGVEKAIYGAFEETFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKVYYNWESGKS
EKCTFCYPRMEVGQPTVCSETCVGRIRYLGVLLYDADRIEAAASTPNEQDLYQAQLDVFLDPNDPKVIEQARADGVPEAW
IKAAQASPVYKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAVAAGALETDAEMPDVASMRIPVRYLANLLTAGKEEP
VVVALERMMAMRKYMRAKTVEGVIDHPTAERVGLTARQIDEMYRYMAIANYEDRFVIPTTHRETSEDAYGLRGDCGFTFG
NDCSGGRTELGLFGKDKRPRAKTKTPMEM
>Mature_509_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKDWENQKRWNGGWRRKKNGRIEPRMGA
KWRILAKIFANPDLPEIDDYYEPFDFDYAHLQTAPEMKAFPTARPRSKITGERMEKIEWGPNWEEILGGEFEHRSADYNF
EGVEKAIYGAFEETFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKVYYNWESGKS
EKCTFCYPRMEVGQPTVCSETCVGRIRYLGVLLYDADRIEAAASTPNEQDLYQAQLDVFLDPNDPKVIEQARADGVPEAW
IKAAQASPVYKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPIQSAVAAGALETDAEMPDVASMRIPVRYLANLLTAGKEEP
VVVALERMMAMRKYMRAKTVEGVIDHPTAERVGLTARQIDEMYRYMAIANYEDRFVIPTTHRETSEDAYGLRGDCGFTFG
NDCSGGRTELGLFGKDKRPRAKTKTPMEM

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=494, Percent_Identity=73.4817813765182, Blast_Score=782, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=70.8835341365462, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=43.4343434343434, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=39.1752577319588, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=35.8695652173913, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=36.7816091954023, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367345, Length=105, Percent_Identity=30.4761904761905, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=35.632183908046, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58125; Mature: 58125

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKDWENQK
CEEHEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RWNGGWRRKKNGRIEPRMGAKWRILAKIFANPDLPEIDDYYEPFDFDYAHLQTAPEMKAF
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSKITGERMEKIEWGPNWEEILGGEFEHRSADYNFEGVEKAIYGAFEETFMMYLP
CCCCCCHHCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKVYYNWESGKS
HHHHHHCCCEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCC
EKCTFCYPRMEVGQPTVCSETCVGRIRYLGVLLYDADRIEAAASTPNEQDLYQAQLDVFL
CCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECCHHHHHHCCCCCHHHHHEEEEEEEE
DPNDPKVIEQARADGVPEAWIKAAQASPVYKMAMEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAVAAGALETDAEMPDVASMRIPVRYLANLLTAGKEEPVVVALERMMAMRKYMRAKTV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGVIDHPTAERVGLTARQIDEMYRYMAIANYEDRFVIPTTHRETSEDAYGLRGDCGFTFG
HHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
NDCSGGRTELGLFGKDKRPRAKTKTPMEM
CCCCCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKDWENQK
CEEHEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RWNGGWRRKKNGRIEPRMGAKWRILAKIFANPDLPEIDDYYEPFDFDYAHLQTAPEMKAF
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSKITGERMEKIEWGPNWEEILGGEFEHRSADYNFEGVEKAIYGAFEETFMMYLP
CCCCCCHHCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKVYYNWESGKS
HHHHHHCCCEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCC
EKCTFCYPRMEVGQPTVCSETCVGRIRYLGVLLYDADRIEAAASTPNEQDLYQAQLDVFL
CCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEECCHHHHHHCCCCCHHHHHEEEEEEEE
DPNDPKVIEQARADGVPEAWIKAAQASPVYKMAMEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAVAAGALETDAEMPDVASMRIPVRYLANLLTAGKEEPVVVALERMMAMRKYMRAKTV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EGVIDHPTAERVGLTARQIDEMYRYMAIANYEDRFVIPTTHRETSEDAYGLRGDCGFTFG
HHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
NDCSGGRTELGLFGKDKRPRAKTKTPMEM
CCCCCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]