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The map label for this gene is gloB

Identifier: 114797885

GI number: 114797885

Start: 3549445

End: 3550215

Strand: Reverse

Name: gloB

Synonym: HNE_3406

Alternate gene names: 114797885

Gene position: 3550215-3549445 (Counterclockwise)

Preceding gene: 114798169

Following gene: 114798676

Centisome position: 95.82

GC content: 60.44

Gene sequence:

>771_bases
ATGAGTGTCCTGATTGAAATACACCAGTTTCCCTGCCTGAACGATAATTACGGCTATCTGGTCCATGAACCCGGTTCGGG
CCGCACGATTGCCATCGATACGCCGGACGCCGCTGTATATCTGGCAGAGGCGGAGAAGATGAGTTGGACCATCAGCGAAA
TCTGGAACACCCACTGGCATCCCGATCATGCGGGGGGAAACCTCAAGATCAAGGAAGCCACCGGCTGCAAGATCATCGGC
CCGGCCGGTGAGGCAGATAAAATCCCCGGCATCGACCGGCGCGTCAAAGGCGGCGATACCGTAGAGCTGGGCGGCGCAAC
GGCGACCGTGATCGATGTGCCGGGCCACACGCTCGGCCATATCGCCTTTCACTTTCCCGATCAGGAAGCCGCCTTCGTCG
GCGACGCTGTCTTCGCGCTGGGCTGTGGCCGCGTTTTCGAGGGCACGATGGAAATGATGTGGGAGAGCCTCAAGCGCATC
AAGAAGCTGCCCAAGAAGACGCAGCTCTATTGCGCGCATGAATACACCGCCTCGAATGCAAAATTCGCCGTCACCATCGA
GCCGGAAAACAAGGCGCTGAAGGAATATGTCGCCTGGATCGAGAAGCGCCGGGCCGAAGACAAGCCCACCGTGCCGGCGC
TGCTGGAGCGGGAGCTGGAGACCAATCCCTTCCTGCGCGCAGACCTTCCCGAAATGCAGCTCGCCATGGGCCATGCGGGC
AATGCGGCCGCCACGTTTGGCGAAATCCGCGGGCGGAAGGATCGCTTCTGA

Upstream 100 bases:

>100_bases
TCGGACGCATAAAAGCGTTCAAGGGTGACGGCGTCCTGTCGCATCGTTATGGGTGGCTCGCGCGTTCCTGTTTCTGGGGG
CCGGTAACTGGAGGTAAGAA

Downstream 100 bases:

>100_bases
TGCGATCTGCCATTTCCGGCGATCTTGGCCCGAATGAAATCATCCGCATGCTTGGCCTGAGGCCCCATCCGGAAGGGGGC
CATTATGGCGAGACATACAG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSVLIEIHQFPCLNDNYGYLVHEPGSGRTIAIDTPDAAVYLAEAEKMSWTISEIWNTHWHPDHAGGNLKIKEATGCKIIG
PAGEADKIPGIDRRVKGGDTVELGGATATVIDVPGHTLGHIAFHFPDQEAAFVGDAVFALGCGRVFEGTMEMMWESLKRI
KKLPKKTQLYCAHEYTASNAKFAVTIEPENKALKEYVAWIEKRRAEDKPTVPALLERELETNPFLRADLPEMQLAMGHAG
NAAATFGEIRGRKDRF

Sequences:

>Translated_256_residues
MSVLIEIHQFPCLNDNYGYLVHEPGSGRTIAIDTPDAAVYLAEAEKMSWTISEIWNTHWHPDHAGGNLKIKEATGCKIIG
PAGEADKIPGIDRRVKGGDTVELGGATATVIDVPGHTLGHIAFHFPDQEAAFVGDAVFALGCGRVFEGTMEMMWESLKRI
KKLPKKTQLYCAHEYTASNAKFAVTIEPENKALKEYVAWIEKRRAEDKPTVPALLERELETNPFLRADLPEMQLAMGHAG
NAAATFGEIRGRKDRF
>Mature_255_residues
SVLIEIHQFPCLNDNYGYLVHEPGSGRTIAIDTPDAAVYLAEAEKMSWTISEIWNTHWHPDHAGGNLKIKEATGCKIIGP
AGEADKIPGIDRRVKGGDTVELGGATATVIDVPGHTLGHIAFHFPDQEAAFVGDAVFALGCGRVFEGTMEMMWESLKRIK
KLPKKTQLYCAHEYTASNAKFAVTIEPENKALKEYVAWIEKRRAEDKPTVPALLERELETNPFLRADLPEMQLAMGHAGN
AAATFGEIRGRKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=33.7164750957854, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=33.7164750957854, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=33.7719298245614, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI116642887, Length=239, Percent_Identity=34.3096234309623, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI21703352, Length=239, Percent_Identity=34.3096234309623, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI46361987, Length=205, Percent_Identity=31.219512195122, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=35.546875, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17536925, Length=252, Percent_Identity=34.1269841269841, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=267, Percent_Identity=33.7078651685393, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324614, Length=243, Percent_Identity=32.0987654320988, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356335, Length=261, Percent_Identity=36.0153256704981, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667711, Length=261, Percent_Identity=36.0153256704981, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24667703, Length=261, Percent_Identity=36.0153256704981, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI221330176, Length=167, Percent_Identity=29.940119760479, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_HYPNA (Q0BWR4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762079.1
- ProteinModelPortal:   Q0BWR4
- SMR:   Q0BWR4
- STRING:   Q0BWR4
- GeneID:   4288675
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3406
- NMPDR:   fig|228405.5.peg.3261
- TIGR:   HNE_3406
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- PhylomeDB:   Q0BWR4
- BioCyc:   HNEP81032:HNE_3406-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28240; Mature: 28109

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLIEIHQFPCLNDNYGYLVHEPGSGRTIAIDTPDAAVYLAEAEKMSWTISEIWNTHWH
CEEEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCEEEEEECCHHEEEHHHHCCCCCC
PDHAGGNLKIKEATGCKIIGPAGEADKIPGIDRRVKGGDTVELGGATATVIDVPGHTLGH
CCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEE
IAFHFPDQEAAFVGDAVFALGCGRVFEGTMEMMWESLKRIKKLPKKTQLYCAHEYTASNA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCC
KFAVTIEPENKALKEYVAWIEKRRAEDKPTVPALLERELETNPFLRADLPEMQLAMGHAG
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHCCCC
NAAATFGEIRGRKDRF
CCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVLIEIHQFPCLNDNYGYLVHEPGSGRTIAIDTPDAAVYLAEAEKMSWTISEIWNTHWH
EEEEEEECCCCCCCCCCEEEECCCCCCEEEEECCCCEEEEEECCHHEEEHHHHCCCCCC
PDHAGGNLKIKEATGCKIIGPAGEADKIPGIDRRVKGGDTVELGGATATVIDVPGHTLGH
CCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCEEE
IAFHFPDQEAAFVGDAVFALGCGRVFEGTMEMMWESLKRIKKLPKKTQLYCAHEYTASNA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEEEEEECCCC
KFAVTIEPENKALKEYVAWIEKRRAEDKPTVPALLERELETNPFLRADLPEMQLAMGHAG
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHCCCC
NAAATFGEIRGRKDRF
CCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA