Click here to switch to the map view.

The map label for this gene is pks16 [H]

Identifier: 114797857

GI number: 114797857

Start: 1624512

End: 1626287

Strand: Reverse

Name: pks16 [H]

Synonym: HNE_1593

Alternate gene names: 114797857

Gene position: 1626287-1624512 (Counterclockwise)

Preceding gene: 114798863

Following gene: 114797677

Centisome position: 43.89

GC content: 61.37

Gene sequence:

>1776_bases
ATGTCTGCATATGTAGACGTTCAGGGGTCTTTCGAATTGAAACCAACTGCTACAGAGGCGGGGATACCCGCGCGTCTCGG
AACTTTCCAGTCGGCTTGCGACGCGTTGGATTACGCGGCTGAGGGGCAGAGCGGTATCAATTTTTATAGCTCTGAAGGCA
AGCTGACGTGCAGCTTGTCTTATCGCGAATTGAAGGATCAGGCGCTAGGCGTGGCCGCCCGGATGGCCGCTCGTTTTCCC
AGAGGTGCGCGAATCGGCGTTATCGCCGAGACCTCTCCTGAGTTCGTCATCACATTCATGGCGTGCCAGTATTCCGGCCT
CGTCCCTGCCCCGCTGAGCCTGCCAGCCGCCTTTGGCGGGCGTCAGACCTATGAGTGGCAGGTCAGCAGCATGGTGCGCA
CAGCTGGCCTCTCGGCCATTTTCGCCCCACCTGCGCTCCACGAAATTCTCAGCTCGGCAACCGCCGGGCAAGACATCGAG
CTGTTCGACCTGAGCGGCGCCACCCTTCCCGATGAAACAGTAGGGCCTGTGCCCCACGGCGCCGGCGATCTGTCCTATAT
CCAGTTTTCATCCGGCAGCACCAGCGAACCGAAAGGCATTGTCGCAACTCAGGCAAGCCTCTCGGCCAACTGCAAGGCCA
TCATTCAGGAAGGCTTGCAGGTGCGCGCCGGCGACCGGGCGGTCTCTTGGTTGCCGCTTTATCACGACATGGGGCTTGTC
GGTTTTTTCATCGCGCCAATGTATTCCCAGTTGTCGATCGACTTCCTGTCGCCCACCGATTTCGCGCGCCGCCCCGGTAC
CTGGTTGAAATTGATCAGCGCCAACAAAGGCACCCTCTCCTACAGCCCGAGCTTTGGTTATGAGCTTTGTGTGCGCCGGT
TCCGCGGCGAACCGCTCGACCTCTCAAGCTGGCGCGCCGCTGGCATTGGCGGCGATATGGTGCGGGCCGATGCGCTCGAT
CAGTTCAGCGAAACGTTTGCCGCATCGGGCTTCCGCAAAGAGGCATTCGTGGCAAGTTATGGCCTGGCGGAATCGACGCT
TGCGGTCAGCTTTGCGCCGCTTAACACCGGCCTGAACATCGACCGGGTCGACGTTGCCCGGATGCAGGCGTCTGAACGCG
CTGTGCCGGCATCGGATTTGACACGCGAGGGCGCCGACCGGGCCTTCGTCAGCTGCGGCAAGCCGCTGGAGTCTACCGAA
TTGAAGATCGTCGATCCCGAAGGCTCAGAGCTTGGCCTGCGTCAGGTCGGCCGGATCTTCATTCGAAGCGCTTCGCTGGC
GTCCGGGTATTTCAGGGCCGACCAGGAACTTCAACCGTTGACGGACAAAGATGGCTGGCTGGATACGGGCGACCTCGGCT
ATTGGCTGGCCGACGAGATCGTCGTGACCGGACGATCGAAGGACATGATCCTCTCGAACGGCCGGAACATCTGGCCGCAG
GATATTGAATGGGTCGCGCAAAAGATCGGTGGACGGACGGTTGCCCGGTCCGCCGCATTTGAAATGCGCGATACAGCCGA
CACTTCGCGCATTGTCCTGCTCGTGGAATGCCGGGCGCAGGAGGCGGAGTTGCGCGAAACGCTGGCGCGGGATATTGCCG
CCGCTGCGCGCTCAATGGCGGGCGCGCCCGTCAGCGTGGAGCTTGTGAGCATAAAGTCGCTTCCTGTAACCAGTTCCGGC
AAGCTGAGCCGCGCGGCTTCCCGCACTGCCTACATATCGGGGCTGATGCATCAGGCTGTGGGTGACAGTGCCACCCGTGC
GGCAGCTCTTCCCTGA

Upstream 100 bases:

>100_bases
AAGATGCAGCAGGATTACCACAAAAGCAGCCTATTGATCTTGACGATAAATGTCAGACGCGTGAATTCATAGCTGAGCCT
GCGCCCATTTCCGGGAGCAG

Downstream 100 bases:

>100_bases
TAAAGCGGCGAGCACCAGATGGTCGACCGGCCACTCATTGCTCTGACAGGCGCGACAGGATTCGTGGGCAGGCAACTTCT
GCGGGATCGGACACAGAACT

Product: putative AMP binding protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSAYVDVQGSFELKPTATEAGIPARLGTFQSACDALDYAAEGQSGINFYSSEGKLTCSLSYRELKDQALGVAARMAARFP
RGARIGVIAETSPEFVITFMACQYSGLVPAPLSLPAAFGGRQTYEWQVSSMVRTAGLSAIFAPPALHEILSSATAGQDIE
LFDLSGATLPDETVGPVPHGAGDLSYIQFSSGSTSEPKGIVATQASLSANCKAIIQEGLQVRAGDRAVSWLPLYHDMGLV
GFFIAPMYSQLSIDFLSPTDFARRPGTWLKLISANKGTLSYSPSFGYELCVRRFRGEPLDLSSWRAAGIGGDMVRADALD
QFSETFAASGFRKEAFVASYGLAESTLAVSFAPLNTGLNIDRVDVARMQASERAVPASDLTREGADRAFVSCGKPLESTE
LKIVDPEGSELGLRQVGRIFIRSASLASGYFRADQELQPLTDKDGWLDTGDLGYWLADEIVVTGRSKDMILSNGRNIWPQ
DIEWVAQKIGGRTVARSAAFEMRDTADTSRIVLLVECRAQEAELRETLARDIAAAARSMAGAPVSVELVSIKSLPVTSSG
KLSRAASRTAYISGLMHQAVGDSATRAAALP

Sequences:

>Translated_591_residues
MSAYVDVQGSFELKPTATEAGIPARLGTFQSACDALDYAAEGQSGINFYSSEGKLTCSLSYRELKDQALGVAARMAARFP
RGARIGVIAETSPEFVITFMACQYSGLVPAPLSLPAAFGGRQTYEWQVSSMVRTAGLSAIFAPPALHEILSSATAGQDIE
LFDLSGATLPDETVGPVPHGAGDLSYIQFSSGSTSEPKGIVATQASLSANCKAIIQEGLQVRAGDRAVSWLPLYHDMGLV
GFFIAPMYSQLSIDFLSPTDFARRPGTWLKLISANKGTLSYSPSFGYELCVRRFRGEPLDLSSWRAAGIGGDMVRADALD
QFSETFAASGFRKEAFVASYGLAESTLAVSFAPLNTGLNIDRVDVARMQASERAVPASDLTREGADRAFVSCGKPLESTE
LKIVDPEGSELGLRQVGRIFIRSASLASGYFRADQELQPLTDKDGWLDTGDLGYWLADEIVVTGRSKDMILSNGRNIWPQ
DIEWVAQKIGGRTVARSAAFEMRDTADTSRIVLLVECRAQEAELRETLARDIAAAARSMAGAPVSVELVSIKSLPVTSSG
KLSRAASRTAYISGLMHQAVGDSATRAAALP
>Mature_590_residues
SAYVDVQGSFELKPTATEAGIPARLGTFQSACDALDYAAEGQSGINFYSSEGKLTCSLSYRELKDQALGVAARMAARFPR
GARIGVIAETSPEFVITFMACQYSGLVPAPLSLPAAFGGRQTYEWQVSSMVRTAGLSAIFAPPALHEILSSATAGQDIEL
FDLSGATLPDETVGPVPHGAGDLSYIQFSSGSTSEPKGIVATQASLSANCKAIIQEGLQVRAGDRAVSWLPLYHDMGLVG
FFIAPMYSQLSIDFLSPTDFARRPGTWLKLISANKGTLSYSPSFGYELCVRRFRGEPLDLSSWRAAGIGGDMVRADALDQ
FSETFAASGFRKEAFVASYGLAESTLAVSFAPLNTGLNIDRVDVARMQASERAVPASDLTREGADRAFVSCGKPLESTEL
KIVDPEGSELGLRQVGRIFIRSASLASGYFRADQELQPLTDKDGWLDTGDLGYWLADEIVVTGRSKDMILSNGRNIWPQD
IEWVAQKIGGRTVARSAAFEMRDTADTSRIVLLVECRAQEAELRETLARDIAAAARSMAGAPVSVELVSIKSLPVTSSGK
LSRAASRTAYISGLMHQAVGDSATRAAALP

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=522, Percent_Identity=23.3716475095785, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI55749758, Length=525, Percent_Identity=23.047619047619, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=393, Percent_Identity=23.9185750636132, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=431, Percent_Identity=22.2737819025522, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653035, Length=189, Percent_Identity=27.5132275132275, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654656, Length=607, Percent_Identity=21.5815485996705, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161076582, Length=98, Percent_Identity=36.734693877551, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356947, Length=98, Percent_Identity=33.6734693877551, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI18859661, Length=437, Percent_Identity=23.1121281464531, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=183, Percent_Identity=24.5901639344262, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63105; Mature: 62974

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYVDVQGSFELKPTATEAGIPARLGTFQSACDALDYAAEGQSGINFYSSEGKLTCSLS
CCEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEC
YRELKDQALGVAARMAARFPRGARIGVIAETSPEFVITFMACQYSGLVPAPLSLPAAFGG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCCCCCHHCCC
RQTYEWQVSSMVRTAGLSAIFAPPALHEILSSATAGQDIELFDLSGATLPDETVGPVPHG
CEEEEHHHHHHHHHHCCHHEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
AGDLSYIQFSSGSTSEPKGIVATQASLSANCKAIIQEGLQVRAGDRAVSWLPLYHDMGLV
CCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHCCCEEECCCCEEEEEEHHHHHHHH
GFFIAPMYSQLSIDFLSPTDFARRPGTWLKLISANKGTLSYSPSFGYELCVRRFRGEPLD
HHHHHHHHHHCEEEECCCCCHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCC
LSSWRAAGIGGDMVRADALDQFSETFAASGFRKEAFVASYGLAESTLAVSFAPLNTGLNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCC
DRVDVARMQASERAVPASDLTREGADRAFVSCGKPLESTELKIVDPEGSELGLRQVGRIF
CHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
IRSASLASGYFRADQELQPLTDKDGWLDTGDLGYWLADEIVVTGRSKDMILSNGRNIWPQ
HHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCEEEECEEEEECCCCCEEEECCCCCCHH
DIEWVAQKIGGRTVARSAAFEMRDTADTSRIVLLVECRAQEAELRETLARDIAAAARSMA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
GAPVSVELVSIKSLPVTSSGKLSRAASRTAYISGLMHQAVGDSATRAAALP
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
SAYVDVQGSFELKPTATEAGIPARLGTFQSACDALDYAAEGQSGINFYSSEGKLTCSLS
CEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEC
YRELKDQALGVAARMAARFPRGARIGVIAETSPEFVITFMACQYSGLVPAPLSLPAAFGG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCCCCCHHCCC
RQTYEWQVSSMVRTAGLSAIFAPPALHEILSSATAGQDIELFDLSGATLPDETVGPVPHG
CEEEEHHHHHHHHHHCCHHEECCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
AGDLSYIQFSSGSTSEPKGIVATQASLSANCKAIIQEGLQVRAGDRAVSWLPLYHDMGLV
CCCCEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHCCCEEECCCCEEEEEEHHHHHHHH
GFFIAPMYSQLSIDFLSPTDFARRPGTWLKLISANKGTLSYSPSFGYELCVRRFRGEPLD
HHHHHHHHHHCEEEECCCCCHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCC
LSSWRAAGIGGDMVRADALDQFSETFAASGFRKEAFVASYGLAESTLAVSFAPLNTGLNI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCC
DRVDVARMQASERAVPASDLTREGADRAFVSCGKPLESTELKIVDPEGSELGLRQVGRIF
CHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
IRSASLASGYFRADQELQPLTDKDGWLDTGDLGYWLADEIVVTGRSKDMILSNGRNIWPQ
HHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCEEEECEEEEECCCCCEEEECCCCCCHH
DIEWVAQKIGGRTVARSAAFEMRDTADTSRIVLLVECRAQEAELRETLARDIAAAARSMA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
GAPVSVELVSIKSLPVTSSGKLSRAASRTAYISGLMHQAVGDSATRAAALP
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]